LOCUS       MR211970                9093 bp    DNA     circular  SYN 
DEFINITION  Akna (Myc-DDK-tagged) - Mouse AT-hook transcription factor (Akna)
ACCESSION   NM_001045514
VERSION     NM_001045514.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5250..5341 
                     /label="Myc-DDK"  
     misc_feature    5371..5956 
                     /label="PolyA signal"  
     misc_feature    5428..5447 
                     /label="Restriction Enzyme"  
     misc_feature    6232..6904 
                     /label="ColE1"  
     misc_feature    7334..8128 
                     /label="Kan/Neo"  
     misc_feature    8163..8465 
                     /label="SV40 ori"  
     misc_feature    8551..9028 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5232..5238 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5232 
                     /label="Akna"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGCTCA GGGGCAAAGG CACAATGGGT TGGGCCAAGC CTGGGGCAAG 
     1081 GGCCCCGAAG GCGGCGCTGG GCTTGGGCTG AGGAACAAGA TACAGATGGA AGAAGAGACC 
     1141 AGGGCTGGGG AAACAGCCAG TCTCTTCCCG AAGCCCCCAG TCCTGAGCTC CTAGAGGACT 
     1201 TCCGTCGGGC ACAGGAGCAC CTGCCACCCC TGGAGTGGGA CCCAGACATG CAGGACTCCG 
     1261 AAGAGTCTTC TGGAGAAGAG ACCGAAGCTG ACGATGCAAG CAGCCCAGAG GGTTCCACCG 
     1321 TGCCTCTGCC CTGGCTCTCC AGGAGCAACC AACAGCTGGA CATGTCTGAA GAGGAGCTGG 
     1381 ATGAGGCCTC TGGGAGGCCT GAGGTAGACT TAGCTGGAGA GAGTTGTACG GAGCTTGAGT 
     1441 GTGAAGACCA GGGAGATTCC AGCCCTCCAC CTCCAGGACA GGGGCCAGCC AAAGGCTGGG 
     1501 TCACCTTTAT CAAACAAGGC AGTAACTACA GACCTTCAGA GCATCTCGAG GCCCAGCCAT 
     1561 CTGTTGAACA TAGCCGCACA AAGTCTTGGA GCAGTGGAAC TGTGAGCCTC CGTCAACCTA 
     1621 GTGACAGCCT TGGTTCTACC TGGGAAGGAG ATACCGAAGT CCCTCAGCCT TCCATCTTGC 
     1681 CCAAAGCCCT GCCACAGAGT CCGTGCCACA ACTTTCCACA CCCAGGTGAC AGAAATGGAG 
     1741 GTGACGTTGC TCCGGCAACA CCCACAGAAT TCCGGGACTC CTTAGCAGCG CCAGCCCAGA 
     1801 ATGCCGAGTG CTCTGCAGGC ACGTGGGGGG AAGAAACCAC CAGCCTACCC AGCTCTCGGC 
     1861 CTGAGGACCA AACCTGGAAG AGGACTAAGA CATCACCTAA GCCACTGCCC TCCCGGTTTA 
     1921 CTGGTTCTGT CAGCCCCCTG AGTACTCGGC TTGGGGCAAT AAAGAAAGTT GTGCCCCAGC 
     1981 ACAAGCAGGG AGCCACTCTG GCTGGTCACT CTTCTTCACA AGCTCCCAAG TATGGCAGGG 
     2041 GGCGCCGCCT GAACTACCCA CTCCCCGACT TCTCCAAGGT GGGGCCCCGG GTGAGGTTTC 
     2101 CCAAAGATGA GAACTACCGA CCCCCGAAGT CCAGAGGCCA CAACAGGCAA CAAGGCTCTA 
     2161 CCAGACCCCT CATCTTCAAG TCGCCAGCTG AGATTGTTCG GGATGTACTG TTGAGTAGTG 
     2221 GAGAGGCGTC CCTGGCCAAG GAATCTTCTC TTGCTCACAC CATCACCAGG GTGCCCCAGG 
     2281 AGTTCCAGAC ACCCGAACAA GCCACTGAGC TAGTTCATCA GCTCCAGGAG GACTATCACA 
     2341 AGCTCCTCAC CAAGTATGCG GAGGCTGAGA ACACTATCGA CCAACTTCGC CTTGGGGCCA 
     2401 AGGTGCACCT GTATTCAGAC CCACCACAGC CCAGTCAGAG CTTCTGCTCG GGGTCAATGC 
     2461 CACAAGGAAG CAAGGTCTTA TCCTTCTCTA TCCCGCAACC CAGAGTAGCA GAGTGGTGGC 
     2521 CTGACCCTGC CCAGGATCCA CAGGCCTCTG AGGCCACAGG GTGGCCGTTC CCACGAACAG 
     2581 ACCTGAGTCC CTCCTCGTCC CCCGGCGTGG CCACTCCAGG GCGGCTTCCT CAGAGCCAGG 
     2641 GCATTGCCAC AGACCAGCCC TCCACAGGAC AGACCCAGGC ACTGACCTCT CAGGCCAGCC 
     2701 GGCTCCTGGC CAAGGTGCAG TCCTTCGAGG AACTGGTACT GGCTGGACAC CTCCCACCTC 
     2761 AGGACCAAAT CAAGAGCTTG GAGCAACTGA GGGCAGCCCA CATGGCCCTA GAGGCAGAGT 
     2821 ACCTGCAGGC CTGCAGAGAA GAGCTCCTGG ACCCTCAGCT TGATGCCTCT CAGGGGAGTC 
     2881 CCAGGACCCT CAACCTCTGC AGGGAGCTAG AAGCAGAGAT ATACCACCTG GGACAGCGCC 
     2941 TGGAAGAGCT ACAGGACCAT ATGGACCAGA CCCAAAGAGA GACAGAGCCA TGTAGGCCAG 
     3001 ACCTGCAAGA CAGCACCCCA ACCATGTCCT TTCTGCCCCA GTCAGCTCAT CTGTCCATGC 
     3061 CTTCAGGACC TGTCTCCTTG CCAGATGGCC AAACCTACCA GGAGCCTGCC ACCACCACCA 
     3121 CCACCTCCCC TGGAAGCTCT TGCACGCTCC CCATCAACAA GAAGTTAAGC CTGAGCATCA 
     3181 AAACTGAGGA AAGCCCAAGA GGTCTCCCGG TCCCACTCAG GGACAGGACG CTTCAGGTGG 
     3241 AGCAGGACTT CCATGGCCTT CTGGAGAGAT ACCTCAGCGT TAAATCCCTC CCTGAAGCCT 
     3301 TGAGGGATGA AGATGAGGAT GACCTGGAGG AGGAGGAAGA GGAGCAGGAC CATCAAGGCC 
     3361 CCTTGGAAGT TGATAGTCCA GCCACAGCTC CAGGGAAAAC AGAAGCCGTG AGGGTACCAC 
     3421 CAGGAGAGCG CCCAACACAG GCTGAGGAAA GCCATAGGGA TGCTACCCAG GAGGATGAGG 
     3481 AACAGATGGG ACCTATGAAG TCACCAGACT TCCGGCCATC CATGGCCAGA GATACGTACA 
     3541 CACCAGTCCT GGATACAGCT GAAGTGGCTC AACGGGGCAC CAAAGCCATG GTGTCACATC 
     3601 AGAGCAGTCT GACCAGCCTG GAAGAGAGCA GGCCCTCAGA GCTCCTTCCT CGAAAAGCCC 
     3661 TGCTTCGGGC TGGTGGGCCC CACACTGAGG AGCCCTGGAT GGTGTCACCA GAGACAGACA 
     3721 GTGGCTTCGT GGGCTCAGAA ACCAGTATAG TGTCACCCTT CACCCAGACC CCAGAGCACC 
     3781 GGCTCTCCCA TGTCAGCACT TCAGGACCAT CAGCTCAACA CCTCACTGCC TCTGTGCCTG 
     3841 GTGATAGAAC CTCCCATCCC AAGGCCAGGG GTCTCATGGT TCCCAGAAGA GCCACTGAGA 
     3901 CTGGCATACC CAGAAGCCGA ACCCAGCAGC ATTTCTCCAG CCTGAGCTCT CCCGGGAGAG 
     3961 GGGCACAGAG CTGCCACCTA GAGGAGACAT CAGTGGCTAA AATAGCGGTT CCCAGGTCTG 
     4021 AGTTCAAGAG ACAGAAGCAG ATTTCCAAAC AGCTCCTTCC CAGTGGAAGA ACCAGCCCCG 
     4081 ACTCTGCCCC AGCTCCCACA GCTGCCTCCA CGCCCCATGG ATCAGCAGAG AGCACTGCCA 
     4141 ACCTCCTCCT CAACAGGACA GAGCGCGACC AGGCCATCAA GGATCTGCAG GCGGAGGTGT 
     4201 CACGGCTCCG CCTTCAACTG GAGGACAGCC TGCACCGGCC ACACCCAGAC GGCCCAGCCT 
     4261 GTGTAGCTTC TGCTTTCAAC CACTCCACCC AGACCCAGGA AAAGCTGGGT TCCTCACCCT 
     4321 CCTGGGGCCC CCACTATGGC AGTAAATCCA CAGAGAGGCT GTCTAGGGAG CCTAATGGTG 
     4381 TAGAGCCAGC TGAGCCAATG GGAAGACGTC GAGCCAGGTC CTCTTCGGTG CCTCGGGATG 
     4441 TACCCAGACT GTACCTAAGT TCTGAATCGG AGTCGCCTGC CCCTCGGCTC TCATCTGAGA 
     4501 AGAGCAGGAC CTTTGAGGAG CACCCCGAGG CTGCACAGTG GGGGACAAGA CCACAGAGCA 
     4561 GCTCGAAGCG GAGGGAAAGA GTTTCCTTCC GGGGTCAATA CACAGGCCAG GAGTACCACA 
     4621 TTCTATCCCC AAAGGCCATC CTGAAGGACA GTGGCACACC GTCCTGTCCC CACTGCCATC 
     4681 CCATTAGGAC CCAGGACACA GGCAGTGCTG TCTCTAGAGA CACCACACGG GGCTCATCTG 
     4741 CCGCTGACAC CCTCCGCTGT GCCCTGTGTG GTGAAGTCAA GTCCTCTGCA GAAGCAGATG 
     4801 GCTCCAGCTC AGGTCCCTCT GAGAAGAACA CCCCCAAAAA GCCTTCCACC CCCATCCTCA 
     4861 AACGGAAGAA CAGGCAGACG GGCTCGCCAG TACGAATGGC CCCTGGACTC TGGTACCTGG 
     4921 CTGCTGCGCC CCCAGCCCCA GCCCCACCGG CTTTGGCCTA CATCTCCTCA GCTCCCATTA 
     4981 TGCCTTATCT ACCACCCACT GTGTACTATG CAGCCCCAGC ACCTACCTCA GCCCAGACAG 
     5041 CTTCCCCGCA GCCTGCTCGG GGACCTCGGA GAACCCGGCA CTCAGTCCAA CTGGGTCTGA 
     5101 ATGACCTGGA GGAGCTGCAG GCCGCACTCA GAGAGGCTGC ACAGGCCGCC GAGAACGTTC 
     5161 GCTCCACCAC CCGCCAGCTG AGCCGCTCCC TGTCTGCGGA CCTGCGCCAT GCCCGTAGCC 
     5221 TGCGAGGCTC CTGCCTTTTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5281 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5341 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5401 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5461 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5521 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5581 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5641 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5701 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5761 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5821 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5881 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5941 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6001 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6061 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6121 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6181 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6241 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6301 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6361 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6421 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6481 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6541 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6601 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6661 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6721 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6781 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6841 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6901 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6961 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7021 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7081 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7141 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7201 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7261 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7321 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7381 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7441 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7501 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7561 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7621 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7681 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7741 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7801 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7861 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7921 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7981 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8041 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8101 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8161 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8221 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8281 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8341 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8401 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8461 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8521 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8581 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8641 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8701 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8761 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8821 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8881 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8941 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9001 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9061 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//