LOCUS       MR211968                9090 bp    DNA     circular  SYN 
DEFINITION  Nid2 (Myc-DDK-tagged) - Mouse nidogen 2 (Nid2)
ACCESSION   NM_008695
VERSION     NM_008695.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5247..5338 
                     /label="Myc-DDK"  
     misc_feature    5368..5953 
                     /label="PolyA signal"  
     misc_feature    5425..5444 
                     /label="Restriction Enzyme"  
     misc_feature    6229..6901 
                     /label="ColE1"  
     misc_feature    7331..8125 
                     /label="Kan/Neo"  
     misc_feature    8160..8462 
                     /label="SV40 ori"  
     misc_feature    8548..9025 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5229..5235 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5229 
                     /label="Nid2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCGGGAC CCGACAGCCG GGTGGCTAAC ACCGCCATCA CCGCTATCGC 
     1081 TGCTGGTGAT GCTGTTGCTG CTGTCGCGAG TCGGGGCTCT GCGTCCCGAC GAGCTCTTTC 
     1141 CTTACGGGGA GTCGTGGGGA GACCAGCTGC TGCCGGAGGG CGACGACGAA AGCTCAGCAG 
     1201 CTGTGAAGCT GGCAATACCC CTGCGTTTCT ACGATGCCCA GTTCAGCAGC CTCTACGTGG 
     1261 GCACCAACGG CATCATCTCC ACCCAGGACT TCCCCAGGGA GACTCAATAT GTAGATGATG 
     1321 ATTTTCCTAC TGACTTCCCA GCCATCGCCC CTTTCCTGGC TGACATCGAC ACTAGTCACA 
     1381 GCAGGGGCCG GATCCTGTAC CGCGAGGACA CCTCCGGAGC TGTGCTGAGC CTGGCTGCCC 
     1441 GCTACGTGCG CACTGGCTTC CCGCTGTCGG GGTCCAGCTT CACCCCCACC CACGCCTTCC 
     1501 TGGCCACCTG GGAGCACGTG GGCGCCTACG AGGAGGTCAG TCGCGGGGCT GCGCCGTCAG 
     1561 GAGAGCTGAA CACTTTCCAG GCTGTTCTGG CATCCGATGA ATCTGATACC TACGCCCTCT 
     1621 TTCTCTACCC TGCCAATGGC CTTCAGTTCT TTGGAACCCG CCCCAAAGAG TCCTACAATG 
     1681 TCCAGCTGCA GCTTCCCGCT AGGGTGGGCT TCTGCCGAGG GGAAGCGGAC GACCTGAAGA 
     1741 GAGAAGCACT GTATTTCAGC CTGACTAACA CCGAGCAGTC TGTGAAGAAT CTCTACCAGC 
     1801 TAAGCAACCT GGGGATTCCT GGAGTGTGGG CTTTTCACAT CGGCAGCAGG TTTGCCCTGG 
     1861 ACAATGTCCG TCCAGCCACA GTTGGAGGTG ACCCTTCCAC AGCCCGTTCC TCAGCACTGG 
     1921 AGCACCCCTT CAGCCATGCT GCAGCCCTGG AGAGCTACAC CGAGGACAGT TTCCATTACT 
     1981 ACGATGAGAA TGAAGAGGAT GTTGAGTACC CACCAGTTGA ACCGGGGGAG GCCCCGGAAG 
     2041 GCCACAGCAG AATTGATGTG TCTTTCAATT CAAAGGCTGA TCCAGGCCTT GTGGACGTCG 
     2101 GCACCTCATC TCCAGGTTCC GACCGCGCTT CTCCTTGGCC ATATCCAGCA CCTGGTAACT 
     2161 GGCCATCCTA CCGGGAAACA GAATCGGCTT CTTTGGACCC TCAAACCAAA CAGGGGCGGC 
     2221 CGGTGGGAGA GGGAGAGGTT CTGGATTTCA GGGACCCAGC GGAGCTTTTG GACCAGATGG 
     2281 GTACCAGAGC CCCAGCTCCT CCAGAGGCAG ATGCAGCTCT CCTAACTCCA GTCAACGAAG 
     2341 ACCTTGGGGG CAGAAACACC CAGTCCTATC CAGAGGCAGG GCCAGTGCCT TCAGAGCCAG 
     2401 ATGTTCCTGT CCCTCCTCTG GAAGGAGAAG TCCTTCCTCA TTACCCCGAG TCTGGTCACG 
     2461 TTCCGCCCCT GAGAGGAGGG AAGTATGTGA TAGGACTGGA GGACCACGTG GGATCTAATG 
     2521 ATCAAGTCTT CACGTATAAT GGCGCCAACC TGGAAACCTG CGAACACAGC CACGGCCGGT 
     2581 GCTCCCAGCA TGCCTTCTGC ACCGACTACA CCACTGGCTT TTGCTGCCAC TGCCAGTCGA 
     2641 GGTTTTACGG AAACGGGAAG CACTGCCTGC CAGAAGGGGC ACCTCACCGA GTGAATGGGA 
     2701 AAGTGAGTGG TCGCCTCCGA GTGGGTCACA TTCCTGTGCA CTTCACTGAC GTGGATCTAC 
     2761 ACGCTTACAT CGTGGGCAAT GATGGCAGAG CCTACACTGC CATCAGCCAT GTCCCACAGC 
     2821 CGGCAGCCCA GGCCCTGCTC CCTGTCCTGC CCATTGGAGG TCTGTTTGGC TGGCTCTTTG 
     2881 CTTTGGAGAA GCCAGGCTCT GAGAATGGCT TCAGCCTCAC AGGTGCTACC TTTGTCCATG 
     2941 ACGTGGAAGT CACTTTTCAC CCTGGAGAGG AGAGGGTTCG TATCACTCAA ACTGCCGAGG 
     3001 GGCTTGACCC AGAAAACTAT CTGAGCATTA AGACCAACAT TGAAGGCCAG GTGCCCTTCA 
     3061 TCCCAGCGAA TTTCACAGCC CACATTACTC CGTACAAAGA GTTTTATCAT TACAGGGACT 
     3121 CAGTCGTGAC GTCCTCCAGT TCCCGAAGTT TCTCTCTCAC TTCTGGTTCT ATTAACCAAA 
     3181 CGTGGTCCTA CCACATTGAC CAGAACATCA CTTACCAGGC GTGCAGGCAC GCCCCCAGAC 
     3241 ACCTGGCCAT CCCTGCCACC CAGCAGCTGA CTGTCGACCG GGCCTTTGCT TTGTACAGTG 
     3301 AGGATGAGGG CGTGCTGAGG TTTGCTGTGA CCAATCAGAT TGGCCCTGTT GAAGTGGACT 
     3361 CAGCCCCTGT TGGAGTGAAT CCTTGCTACG ACGGAAGCCA CACCTGTGAC ACAACAGCAA 
     3421 GGTGCCACCC GGGAACAGGT GTAGACTACA CCTGCGAGTG CACGCCTGGG TTCCAGGGTG 
     3481 ACGGACGGAG CTGTGTGGAT GTCAATGAGT GTGCCACTGG CTTCCACCGC TGTGGCCCTA 
     3541 ATTCTGTGTG TGTCAACTTG GTGGGAAGCT ACAGGTGTGA ATGCCGCAGT GGCTATGAAT 
     3601 TTGCAGATGA CCAGCACACT TGTATCTTGA TCGCCCCACC TCCCAACCCT TGCCTCGATG 
     3661 GCAGTCACAC CTGTGCCCCT GAGGGGCAAG CCAGGTGCAT TCACCATGGA GGCAGCTCAT 
     3721 TCAGTTGTGC CTGCCTGCCA GGCTTCATTG GCACTGGGCA TCAGTGTTCT GATGTTGATG 
     3781 AATGTGCGGA AAACCGATGT CATGAGGCAG CTATCTGCTA CAATACCCCC GGGTCCTTCT 
     3841 CCTGCCGTTG CCAGCCTGGG TATCGAGGGG ACGGGTTTCA CTGCACCTCT GACACGGTTC 
     3901 CGGAAGATTC CATCTCAGGA CTGAAGCCCT GTGAATACCA GCAGCGCTAT GCTCAGACAC 
     3961 AGCACGCCTA CCCTGGGTCA CGGATCCACA TCCCCCAGTG TGATGACCAG GGAAACTTCG 
     4021 TGCCACTGCA GTGCCACGGC AGCACTGGCT TCTGCTGGTG TGTGGACCGA AATGGCCACG 
     4081 AAGTCCCTGG CACGCAGACT CCACCCGGCT CCACCCCGCC CCACTGTGGA CCACCTCCAG 
     4141 AGCCCACCCA GAGGCCTCGG ACTGTCTGTG AGCGTTGGCG GGAAAGCCTG CTGGAACACT 
     4201 ACGGAGGCAC ACCCAGGGAT GACCAGTATG TGCCCCAGTG TGATGACCTG GGCCACTTCA 
     4261 TCCCCCTGCA GTGCCATGGA AAGAGTGATT TCTGTTGGTG TGTGGACAAG GATGGCAGAG 
     4321 AGCTACAGGG CACGCGCTCA CAGCCAGGCA CCAGGCCTGC ATGCATACCC ACCGTCGCTC 
     4381 CACCCGTGGT CCGGCCCACA CCCCGGCCCG ATGTGACTCC TCCATCTGTG GGCACCTTCC 
     4441 TGCTCTATGC CCAGGGCCAA CAGATTGGCC ACTTGCCCCT CAATGGCAGC AGGCTTCAGA 
     4501 AGGACGCAGC CCGGACCCTG CTGTCACTGC ATGGCTCCAT AGTTGTGGGG ATTGACTATG 
     4561 ACTGCCGGGA GAGGATGGTC TACTGGACAG ACGTTGCTGG TCGGACCATC AGCCGTGCCA 
     4621 GCTTGGAGGC AGGAGCCGAA CCCGAGACCA TCATTACCTC AGGTTTGATA AGCCCGGAAG 
     4681 GACTTGCCAT CGACCACTTC CGTAGAACAA TGTACTGGAC AGATAGTGGC CTGGATAAGA 
     4741 TAGAGCGGGC CGAACTGGAT GGTTCCGAGC GGAAAGTCCT CTTCCACACA GATCTGGTGA 
     4801 ATCCACGAGC CATCACTGTG GATCCAATCC GAGGCAACTT GTACTGGACA GACTGGAATA 
     4861 GAGAAGCTCC TAAAATTGAA ACATCATCTT TAGATGGCGA AAACAGAAGG ATTCTGATCA 
     4921 ACAAAGATAT TGGATTACCC AATGGATTGA CCTTTGACCC CTTCTCCAAA CTGCTCTGCT 
     4981 GGGCAGATGC AGGAACCAAA AAACTGGAGT GTACACTACC CGATGGAACT GGACGACGTG 
     5041 TCATCCAAAA CCACCTCAAT TACCCCTTCA GCATCGTCAG CTATGCAGAT CACTTCTACC 
     5101 ACACGGACTG GCGGAGGGAT GGTGTTATAT CAGTGAATAA GGACAGTGGC CAGTTTACTG 
     5161 ATGAGTTTCT CCCTGAGCAG CGGTCTCATC TCTATGGGAT CACTGCAGTC TACCCGTACT 
     5221 GTCCAACAGG AAGAAAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5281 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5341 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5401 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5461 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5521 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5581 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5641 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5701 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5761 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5821 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5881 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5941 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6001 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6061 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6121 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6181 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6241 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6361 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6421 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6481 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6541 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6661 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6721 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6781 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6841 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6901 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6961 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7021 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7081 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7141 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7201 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7261 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7321 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7381 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7441 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7501 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7561 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7621 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7681 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7741 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7801 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7861 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7921 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7981 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8041 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8101 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8161 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8221 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8281 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8341 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8521 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//