LOCUS       MR211966                9084 bp    DNA     circular  SYN 
DEFINITION  Wrn (Myc-DDK-tagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 2
ACCESSION   NM_001122822
VERSION     NM_001122822.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5241..5332 
                     /label="Myc-DDK"  
     misc_feature    5362..5947 
                     /label="PolyA signal"  
     misc_feature    5419..5438 
                     /label="Restriction Enzyme"  
     misc_feature    6223..6895 
                     /label="ColE1"  
     misc_feature    7325..8119 
                     /label="Kan/Neo"  
     misc_feature    8154..8456 
                     /label="SV40 ori"  
     misc_feature    8542..9019 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5223..5229 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5223 
                     /label="Wrn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAACCACT TCACTACAGC GGAAATTTCC AGAATGGATG TCTATGCAGA 
     1081 GTCAAAGATG TGCTACAGAA GAAAAGGCCT GCGTTCAGAA GAGTGTTCTT GAAGATAACC 
     1141 TCCCATTCTT AGAATTCCCT GGATCCATTG TTTACAGTTA TGAAGCTAGT GATTGCTCCT 
     1201 TCCTGTCTGA AGACATTAGC ATGCGTCTGT CTGATGGCGA TGTGGTGGGA TTTGACATGG 
     1261 AATGGCCGCC CATATACAAG CCAGGGAAAA GAAGCAGAGT CGCAGTGATC CAGTTGTGTG 
     1321 TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCCAT GTCAGTTTTC CCCCAGGGAT 
     1381 TAAAAATGTT ACTAGAAAAC AAATCAATTA AGAAGGCAGG GGTTGGGATT GAAGGGGACC 
     1441 AGTGGAAACT TCTGCGTGAT TTTGACGTCA AGTTGGAGAG TTTTGTGGAG CTGACGGATG 
     1501 TTGCCAATGA AAAGTTGAAG TGCGCAGAGA CCTGGAGCCT CAATGGTCTG GTTAAACACG 
     1561 TCTTAGGGAA ACAACTTTTG AAAGACAAGT CCATCCGCTG CAGCAATTGG AGTAATTTCC 
     1621 CCCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT CTTATCATCT 
     1681 ATCAAAAATT AGGAAATTTG GGTGATACTG CGCAAGTGTT TGCTCTAAAT AAAGCAGAGG 
     1741 AAAACCTACC TCTGGAGATG AAGAAACAGT TGAATTCAAT CTCCGAAGAA ATGAGGGACC 
     1801 TAGCCAATCG TTTTCCTGTC ACTTGCAGAA ATTTGGAAAC TCTCCAGAGG GTTCCTGTAA 
     1861 TATTGAAGAG TATTTCAGAA AATCTCTGTT CATTGAGAAA AGTGATCTGT GGTCCTACAA 
     1921 ACACTGAGAC TAGACTGAAG CCGGGCAGTA GTTTTAATTT ACTGTCATCA GAGGATTCAG 
     1981 CTGCTGCTGG AGAAAAAGAG AAACAGATTG GAAAACATAG TACTTTTGCT AAAATTAAAG 
     2041 AAGAACCATG GGACCCAGAA CTTGACAGTT TAGTGAAGCA AGAGGAGGTT GATGTATTTA 
     2101 GAAATCAAGT GAAGCAAGAA AAAGGTGAAT CTGAAAATGA AATAGAAGAC AATCTGTTGA 
     2161 GAGAAGATAT GGAAAGAACT TGTGTGATTC CTAGTATTTC AGAAAATGAA CTCCAAGATT 
     2221 TGGAACAGCA AGCTAAAGAA GAAAAATATA ATGATGTTTC TCACCAACTT TCTGAGCATT 
     2281 TATCTCCCAA TGATGATGAG AATGACTCCT CCTATATAAT TGAAAGTGAT GAAGATTTGG 
     2341 AAATGGAGAT GCTGAAGTCT TTAGAAAACC TAAATAGTGA CGTGGTGGAA CCCACTCACT 
     2401 CTACATGGTT GGAAATGGGA ACCAATGGGC GTCTTCCTCC TGAGGAGGAA GATGGACACG 
     2461 GAAATGAAGC CATCAAAGAG GAGCAGGAAG AAGAGGACCA TTTATTGCCG GAACCCAACG 
     2521 CAAAGCAAAT TAATTGCCTC AAGACCTATT TCGGACACAG CAGTTTTAAA CCGGTTCAGT 
     2581 GGAAAGTCAT CCATTCTGTA TTAGAAGAGA GAAGAGATAA TGTTGTTGTC ATGGCAACTG 
     2641 GATATGGGAA GAGTCTGTGC TTCCAGTATC CGCCTGTTTA TACAGGCAAG ATTGGCATTG 
     2701 TCATTTCACC TCTCATTTCC TTAATGGAAG ACCAAGTCCT CCAGCTTGAG CTGTCCAATG 
     2761 TTCCAGCCTG TTTACTTGGA TCTGCACAGT CAAAAAATAT TCTAGGAGAT GTTAAATTAG 
     2821 GCAAATATAG GGTCATCTAC ATAACTCCAG AGTTCTGTTC TGGTAACTTG GATCTACTCC 
     2881 AGCAACTTGA CTCTAGTATT GGCATCACTC TCATTGCTGT GGATGAGGCT CACTGCATTT 
     2941 CAGAGTGGGG CCATGATTTC AGAAGTTCAT TCAGGATGCT GGGCTCTCTT AAAACAGCGC 
     3001 TCCCATTGGT TCCAGTCATT GCACTCTCCG CTACTGCAAG CTCTTCCATC CGGGAAGACA 
     3061 TTATAAGCTG CTTAAACCTG AAAGACCCTC AGATCACCTG CACTGGATTT GATCGGCCAA 
     3121 ATCTGTACTT AGAAGTTGGA CGGAAAACAG GGAACATCCT TCAGGATCTA AAGCCGTTTC 
     3181 TCGTCCGAAA GGCAAGTTCT GCCTGGGAAT TTGAAGGTCC AACCATCATC TATTGTCCTT 
     3241 CGAGAAAAAT GACAGAACAA GTTACTGCTG AACTTGGGAA ACTGAACTTA GCCTGCAGAA 
     3301 CATACCACGC TGGCATGAAA ATTAGCGAAA GGAAGGACGT TCATCATAGG TTCCTGAGAG 
     3361 ATGAAATTCA GTGTGTTGTA GCTACTGTAG CTTTTGGAAT GGGCATTAAT AAAGCTGACA 
     3421 TTCGCAAAGT TATTCATTAT GGTGCGCCTA AGGAAATGGA ATCCTATTAC CAGGAAATTG 
     3481 GTAGAGCTGG CCGGGATGGA CTTCAGAGTT CCTGTCACTT GCTCTGGGCT CCAGCAGACT 
     3541 TTAACACATC CAGGAATCTC CTTATTGAGA TTCACGATGA AAAGTTCCGG TTATATAAAT 
     3601 TAAAGATGAT GGTAAAGATG GAAAAATACC TTCACTCCAG TCAGTGTAGG CGACGAATCA 
     3661 TCTTGTCCCA TTTTGAGGAC AAATGTCTGC AGAAGGCCTC CTTGGACATT ATGGGAACTG 
     3721 AAAAATGCTG TGATAATTGC AGGCCCAGGC TGAATCATTG CCTTACTGCT AACAACTCAG 
     3781 AGGACGCATC CCAAGACTTT GGGCCACAAG CATTCCAGCT ACTGTCTGCT GTGGACATCC 
     3841 TGCAGGAGAA ATTTGGAATT GGGATTCCGA TCTTATTTCT CCGAGGATCT AATTCTCAGC 
     3901 GTCTTCCTGA TAAATATCGG GGTCACAGGC TCTTTGGTGC TGGAAAGGAG CAAGCAGAAA 
     3961 GTTGGTGGAA GACCCTTTCT CACCATCTCA TAGCTGAAGG ATTCTTGGTA GAAGTTCCCA 
     4021 AGGAAAACAA ATATATAAAG ACATGTTCCC TCACAAAAAA GGGTAGAAAG TGGCTTGGAG 
     4081 AAGCCAGTTC GCAGTCTCCT CCGAGCCTTC TCCTTCAAGC TAATGAAGAG ATGTTTCCAA 
     4141 GGAAAGTTCT GCTACCAAGT TCTAATCCTG TATCTCCAGA AACGACGCAA CATTCCTCTA 
     4201 ATCAAAACCC AGCTGGATTA ACTACCAAGC AGTCTAATTT GGAGAGAACG CATTCTTACA 
     4261 AAGTGCCTGA GAAAGTTTCT TCTGGGACTA ACATTCCTAA AAAAAGTGCC GTGATGCCGT 
     4321 CACCAGGAAC ATCTTCCAGC CCCTTAGAAC CTGCCATCTC AGCCCAAGAG CTGGACGCTC 
     4381 GGACTGGGCT ATATGCCAGG CTGGTGGAAG CAAGGCAGAA ACACGCTAAT AAGATGGATG 
     4441 TACCTCCAGC TATTTTAGCA ACAAACAAGG TTCTGCTGGA CATGGCTAAA ATGAGACCGA 
     4501 CTACTGTTGA AAACATGAAA CAGATCGACG GTGTCTCTGA AGGCAAAGCT GCTCTGTTGG 
     4561 CCCCTCTGTT GGAAGTCATC AAACATTTCT GTCAAGTAAC TAGTGTTCAG ACAGACCTCC 
     4621 TTTCCAGTGC CAAACCTCAC AAGGAACAGG AGAAAAGTCA GGAGATGGAA AAGAAAGACT 
     4681 GCTCACTCCC CCAGTCTGTG GCCGTCACAT ACACTCTATT CCAGGAAAAG AAAATGCCCT 
     4741 TACACAGCAT AGCTGAGAAC AGGCTCCTGC CTCTCACAGC AGCCGGCATG CACTTAGCCC 
     4801 AGGCGGTGAA AGCCGGCTAC CCCCTGGATA TGGAGCGAGC TGGCCTGACC CCAGAGACTT 
     4861 GGAAGATTAT TATGGATGTC ATCCGAAACC CTCCCATCAA CTCAGATATG TATAAAGTTA 
     4921 AACTCATCAG AATGTTAGTT CCTGAAAACT TAGACACGTA CCTCATCCAC ATGGCGATTG 
     4981 AGATTCTTCA GAGTGGTTCC GACAGCAGAA CCCAGCCTCC TTGTGATTCC AGCAGGAAGA 
     5041 GGCGTTTCCC CAGCTCTGCA GAGAGTTGTG AGAGCTGTAA GGAGAGCAAA GAGGCGGTCA 
     5101 CCGAGACCAA GGCATCATCT TCAGAGTCAA AGAGAAAATT ACCCGAGTGG TTTGCCAAAG 
     5161 GAAATGTGCC CTCAGCTGAT ACCGGCAGCT CATCATCAAT GGCCAAGACC AAAAAGAAAG 
     5221 GTCTCTTTAG TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5281 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5341 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5401 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5461 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5521 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5581 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5641 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5701 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5761 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5821 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5881 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5941 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6001 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6061 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6121 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6181 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6241 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6301 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6361 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6421 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6481 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6541 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6601 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6661 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6721 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6781 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6841 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6901 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6961 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7021 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7081 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7141 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7201 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7261 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7321 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7381 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7441 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7501 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7561 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7621 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7681 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7741 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7801 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7861 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7921 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7981 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8041 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8101 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8161 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8221 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8281 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8341 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8401 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8461 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8521 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8581 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8641 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8701 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8761 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8821 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8881 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8941 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9001 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9061 TAACAAAAAT TTAACGCGAA TTTT
//