LOCUS       MR211958                9051 bp    DNA     circular  SYN 
DEFINITION  Edc4 (Myc-DDK-tagged) - Mouse enhancer of mRNA decapping 4 (Edc4)
ACCESSION   NM_181594
VERSION     NM_181594.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5208..5299 
                     /label="Myc-DDK"  
     misc_feature    5329..5914 
                     /label="PolyA signal"  
     misc_feature    5386..5405 
                     /label="Restriction Enzyme"  
     misc_feature    6190..6862 
                     /label="ColE1"  
     misc_feature    7292..8086 
                     /label="Kan/Neo"  
     misc_feature    8121..8423 
                     /label="SV40 ori"  
     misc_feature    8509..8986 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5190..5196 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5190 
                     /label="Edc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCCTGC GCGAGCATCG ACATTGAGGA CGCCACGCAG CATCTGCGGG 
     1081 ACATACTCAA ACTGGACCGG CCCGCCGGAG GTTCCAATGC CGAAAGCCAA AGGCCATCCA 
     1141 GTGCCTACAA TGGAGACCTC AATGGGCTCC TGGTCCCCGA CCCGCTCAGT TCAGGTGATG 
     1201 GTAACTCAAC GAACAAACCT GGTATACGGA CCATGCCACC CATTAACCTG CAGGAAAAGC 
     1261 AGGTCATTTG CCTCTCTGGA GATGACAGCT CCACCTGCAT TGGGATTTTA GCCAAGGAAG 
     1321 TGGAAATTGT GGCCAGCAGT GATTCTAGCA TTTCCAGCAA GGCTCGGGGG AGCAACAAGG 
     1381 TGAAAATCCA GCCTGTCGCC AAGTATGACT GGGAGCAGAA ATACTACTAT GGCAACCTGA 
     1441 TTGCAGTGTC TAACTCCTTC TTGGCATACG CCATTAGGGC TGCCAACAAT GGCTCAGCAA 
     1501 TGGTCCGTGT GATCAGTGTC AGCACTTCGG AGCGGACCCT GCTCAAAGGT TTCACAGGCA 
     1561 GTGTGGCTGA TCTTGCTTTT GCACACCTCA ACTCTCCACA GCTCGCCTGC CTGGATGAGG 
     1621 CTGGCAATCT TTTTGTGTGG CGCTTGGCTC TTGTTAAGGG TAAAATTCAA GAAGAGATTT 
     1681 TAGTCCATAT CCGGCAGCCA GAGGGTACAG CGCTGAACCA CTTCCGAAGG ATCATCTGGT 
     1741 GCCCTTTCAT CCCCGAGGAG AGTGAGGACT GTTGTGAGGA GAGCAGCCCG ACAGTGGCCC 
     1801 TGCTGCATGA GGATCGGGCT GAGGTGTGGG ACTTGGATAT GCTTCGCTCC AGCCACAACA 
     1861 CCTGGCCTGT GGATGTCAGC CAAATCAAGC AAGGCTTTAT TGTGGTGAAA GGCCATAGCA 
     1921 CGTGCCTAAG TGAAGGAGCC CTCTCCCCTG ATGGGACTGT CCTGGCTACC GCAAGCCATG 
     1981 ATGGCTTTGT CAAGTTCTGG CAGATCTATA TTGAAGGTCA GGATGAGCCA AGGTGTCTGC 
     2041 ATGAATGGAA GCCTCATGAT GGGAGGCCTC TTTCTTGCCT CCTGTTCTGT GATAACCATA 
     2101 AGAAACAGGA TCCTGAGGTC CCGTTCTGGA GGTTCCTCAT AACCGGCGCC GACCAGAATC 
     2161 GAGAGCTGAA GATGTGGTGC ACAGTGTCCT GGACCTGCCT GCAAACCATT CGCTTCTCCC 
     2221 CAGATATCTT CAGCTCAGTG AGTGTGCCTC CCAGCCTCAA AGTTTGTTTG GACCTCTCAG 
     2281 CAGAGTATTT GATTCTTAGT GATGTGCAAC GAAAGGTCCT CTATGTGATG GAGCTGCTGC 
     2341 AGAACCAGGA CGAGGGCCGC GCGTGCTTTA GTTCTATCTC TGAGTTCCTG CTCACCCACC 
     2401 CTGTGCTGAG CTTTGGCATC CAGGTTGTCA GTCGCTGCCG GCTACGGCAC ACTGAGGTCC 
     2461 TGCCTGCTGA GGAGGAGAAT GACAGTCTGG GGACCGAGAG TTCCCATGGA GCTGGAGCCT 
     2521 TGGAATCCGC AGCTGGTGTG CTTATCAAGC TCTTTTGTGT GCACACTAAG GCACTACAAG 
     2581 ATGTGCAGAT CCGCTTCCAG CCACAGCTGA ACCCTGATGT GGTGGCCCCA CTCTCTACCC 
     2641 ACACTGCCCA CGAGGACTTC ACATTTGGAG AGTCTCGACC TGAACTAGGC TCCGAGGGGC 
     2701 TAGCTTCAGC TGCTCATGGT TCCCAGCCTG ACCTGCGGCG CATTGTGGAA CTGCCTGCTC 
     2761 CTGCAGACTT CCTCAGTCTG AGCAGTGAGA CCAAGCCCAA GTTGATGACA CCTGATGCTT 
     2821 TCATGACACC TACTGCCTCC CTGCAGCAGA TCTCTGCATC CCCTAGTAGT AGCAGCAGCA 
     2881 GTAGCAGCAG CAGCAGTAGC AGCAGCAGCA GCAGCAGCAG CTCTCTAACA GCTGTGTCTG 
     2941 CTGTGAGCAG CTCCTCAGCC ATGGACCCTT CCTTGCCCAG ACCACCTGAG GAGCTGACCT 
     3001 TGAGCCCCAA GCTGCAGCTA GATGGCAGCC TGACGCTAAA CAGCAGCAGC AGCAGCCTGC 
     3061 AGGCAAGCCC TCGAAGCCTC CTTCCGGGCC TGCTCCCAGG CCCAGCTGAC AAGTTGATTT 
     3121 CCAAGGGACC TGGTCAGGTA TCCACTGCTG CCTCTGCACT GTCCTTGGAT TTGCAGGAAG 
     3181 TGGAGCCATT AGGGCTACCC CAGGCCTCCC CAAGTCGAAC CCGTTCTCCT GATGTGATCT 
     3241 CCTCAGCATC CACTGCCCTG TCCCAGGACA TTCCTGAAAT CGCATCTGAG GCCCTGTCCC 
     3301 GTGGCTTTGG CTCCTCTGTT CCTGAGGGCC TCATTGAGCC AGACAGCATG GCCTCAGCTG 
     3361 CCTCAGCACT ACACCTACTC TCTCCTCGGC CCAGGCAAGG CCCTGAGCTT GGCTCTCAGC 
     3421 TGGGTCTGGA TGGAGGCCCT GGAGATGGGG ATAGGCATAG TACCCCTTCC CTGCTGGAAG 
     3481 CAGCCCTGAC CCAGGAAGTT GCAACCCCTG ACAGTCAAGT CTGGCCTACG GCACCTGACA 
     3541 TTACTCGTGA GACCTGTAGC ACACTGACAG AAAGGCCACC TTATCACTTG CTGCAGCAAC 
     3601 GTGACAGTCA GGACACAAGT GCTGAGCAAA GTGACCATGA TGATGAGGTT GCCAGCCTTG 
     3661 CCTCTGCCTC AGGAGGTTTT GGCAGCAAAA TTCCTACTCC ACGGCTGCCT TCCAAGGATT 
     3721 GGAAGACGAA GGGATCCCCT CGGACGTCAC CTAAGCTCAA GAGAAAAAGC AAGAAGGATG 
     3781 ATGGGGATTC AGCTGTGGGA TCCCGGCTCA CAGAGCACCA GGTGGCAGAG CCCCCTGAAG 
     3841 ACTGGCCAGC ACTAATTTGG CAGCAGCAAA GAGAGCTGGC AGAGCTATGG CACAACCAAG 
     3901 AAGAACTGCT ACAGCGTCTC TGTGCCCAGC TTGAAGGTCT TCAGAGTACT GTCACAGACC 
     3961 ATGTAGAGCG TGCCCTGGAG ACGCGGCATG AGCAGGAGCA ACGGCGGCTG GAACGGGCAC 
     4021 TGGCTGAGGG GCAGCAACGG GGTGGGCAGC TGCAGGAGCA GCTGACACAG CAGCTATCCC 
     4081 AGGCATTGTC TTCAGCTGTG GCTGGGCGGC TAGAGCGCAG CGTAAGGGAT GAAATCAAGA 
     4141 AAACGGTTCC TCCATGTGTC TCCAGAAGTC TGGAGCCTGT TGCAGGTCAA CTAAGCAACT 
     4201 CAGTGGCTAC CAAGCTTACA GCTGTGGAAG GCAGCATGAA AGAGAATATC TCTAAGCTAC 
     4261 TCAAGTCCAA GAACTTAACA GATGCCATTG CCCGAGCAGC TGCAGACACG TTACAGGGAC 
     4321 CAATGCAGGC TGCCTACCGG GAAGCCTTCC AGAGTGTGGT ACTGCCAGCT TTTGAGAAGA 
     4381 GTTGCCAGGC TATGTTCCAG CAAATCAATG ACAGCTTTAG GCTGGGCACA CAGGAATATT 
     4441 TGCAGCAGCT GGAGAGTCAC ATGAAGAGCC GGAAGGCCCG TGAACAAGAA GCTAGGGAGC 
     4501 CTGTGCTGGC CCAGCTTCGG GGCCTGGTCA GCACACTGCA GAGTGCCACC GAGCAGATGG 
     4561 CGGCCACTGT ATCTAGCAGT GTTCGGGCCG AGGTGCAACA CCAGCTGCAT GTGGCTGTGG 
     4621 GCAGCTTGCA GGAGTCAATC TTAGCACAAG TACAGCGCAT TGTCAAGGGT GAAGTGAGTG 
     4681 TGGCACTTAA GGAGCAGCAG GCCACCGTCA CTTCCAGCAT CATGCAGGCT ATGCGTTCAG 
     4741 CTGCTGGCAC ACCTGTCCCC TCTGCCCACC TTGACTGCCA GGCCCAACAA GCCCATATCT 
     4801 TGCAGTTACT GCAGCAGGGC CACCTCAATC AGGCCTTCCA GCAGGCACTG ACTGCTGCTG 
     4861 ATCTCAATCT GGTGCTGTAT GTGTGTGAAA CTGTCGACCC AGCCCAGGTT TTTGGGCAGC 
     4921 CGCCCTGTCC ACTATCCCAG CCTGTTCTCC TTTCCCTAAT CCAGCAGCTA GCATCTGACC 
     4981 TTGGAACTAG AAGTGACCTC AAGCTCAGCT ACTTGGAAGA AGCTGTGATG CACCTGGACC 
     5041 ACAGCGATCC CATCACTCGA GACCACATGG GCTCTGTCAT GGCTCAGGTG CGCCAGAAGC 
     5101 TCTTCCAGTT CCTGCAGGCT GATCCACACA ACTCACTTAG CAAAGCTGCC CGCCGCCTCA 
     5161 GCCTAATGTT ACACGGCCTT GTGACCCCTA GCCTCCCTAC GCGTACGCGG CCGCTCGAGC 
     5221 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5281 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5341 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5401 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5461 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5521 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5581 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5641 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5701 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5761 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5821 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5881 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5941 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6001 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6061 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6121 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6181 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6241 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6301 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6361 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6421 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6481 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6541 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6601 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6661 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6721 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6781 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6841 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6901 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6961 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7021 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7081 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7141 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7201 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7261 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7321 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7381 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7441 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7501 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7561 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7621 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7681 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7741 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7801 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7861 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7921 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7981 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8041 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8101 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8161 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8221 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8281 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8341 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8401 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8461 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8521 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8581 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8641 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8701 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8761 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8821 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8881 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8941 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9001 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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