LOCUS       MR211949                9000 bp    DNA     circular  SYN 
DEFINITION  Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2
ACCESSION   NM_027347
VERSION     NM_027347.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5155..5246 
                     /label="Myc-DDK"  
     misc_feature    5276..5861 
                     /label="PolyA signal"  
     misc_feature    5333..5352 
                     /label="Restriction Enzyme"  
     misc_feature    6137..6809 
                     /label="ColE1"  
     misc_feature    7239..8033 
                     /label="Kan/Neo"  
     misc_feature    8068..8370 
                     /label="SV40 ori"  
     misc_feature    8456..8933 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5130..5137 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5130 
                     /label="Med23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTACCGATG GAGACGCAAC TGCAGAGCAT TTTCGAGGAG GTGGTGAAAA 
     1081 CAGAAATTAT AGAAGAGGCC TTCCCAGGAA TGTTTATGGA TACCCCCGAG GATGAAAAAA 
     1141 CAAAACTAAT TAGCTGCTTG GCGGCCTTCC GGCAGTTTTG GAGTGGACTT TCTCAGGAGT 
     1201 CTCATGAACA GTGTGTTCAG TGGATTGTTA AATTTATTCA TGGCCAACAC AGCCCCAAAA 
     1261 GAATTTCTTT CCTCTATGAC TGCTTAGCAA TGGCAGTTGA GACTGGTCTC CTTCCACCCA 
     1321 GGATGGTTTG TGAATCTTTG ATAAACTCTG ACTCTCTTGA ATGGGAAAGA ACACAGCTTT 
     1381 GGGCCTTAAC ATTTAAGCTG GTTCGGAAAA TCATTGGAGG AGTGGATTAC AAGGGTGTTC 
     1441 GAGACCTTCT CAAAGCGATT TTGGAGAAAA TCTTGACAAT CCCAAATACA GTGAGCTCTG 
     1501 CTGTTGTCCA GCAGCTTCTG GCAGCAAGAG AGGTTATAGC TTATATCTTA GAACGAAATG 
     1561 CCTGCCTATT GCCGGCCTAC TTTGCAGTCA CAGAGATCAG AAAACTATAT CCTGAGGGAA 
     1621 AACTTCCACA CTGGTTACTA GGAAACTTGG TATCAGACTT TGTGGATACC TTCAGGCCCA 
     1681 CAGCAAGGAT TAACTCCATT TGTGGCCGAT GCAGTCTTCT GCCAGTTGTG AACAACTCGG 
     1741 GAGCCATCTG TAACTCATGG AAACTGGACC CTGCAACTCT GCGATTTCCT TTGAAAGGCC 
     1801 TTTTGCCATA TGATAAGGAT CTGTTTGAAC CGCAGACTGC TTTGTTGAGA TATGTACTGG 
     1861 AGCAACCTTA TTCCAGGGAT ATGGTCTGCA ATATGCTAGG TTTAAATAAG CAGCACAAGC 
     1921 AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGACCTGGT GGTGTACGCC ATGGAGAGGT 
     1981 CAGAGACCGA GGAGAAGTTT GACGATGGGG GAACCAGCCA GCTCCTGTGG CAGCACCTTT 
     2041 CCAGCCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCCAG TTTCCCACAT ATGGTCCTCT 
     2101 CTCTCCACCA GAAGTTAGCA GGGCGAGGAC TGATTAAAGG GCGAGACCAT CTGATGTGGG 
     2161 TGCTGCTGCA GTTCATTTCT GGAAGTATTC AGAAAAATGC CCTCGCTGAC TTCCTCCCTG 
     2221 TCATGAAGCT CTTTGACCTG CTGTACCCGG AGAAGGAGTG TATCCCAGTT CCTGATATTA 
     2281 ACAAACCCCA GTCAACGCAC GCCTTTGCAA TGACTTGTAT TTGGATTCAT CTTAATAGAA 
     2341 AAGCTCAAAA TGGCGACTCC ACGCTCCAGA TCCCAATACC CCATTCCCTG AAGCTGCACC 
     2401 ATGAGTTCCT CCAGCAGAGT CTGAGAAATA AAAGCTTACA GATGAATGAC TATAAGATTG 
     2461 CCCTGCTATG TAACGCGTAT TCCACAAACT CAGAGTGTTT TACGTTACCT ATGGGAGCTC 
     2521 TGGTAGAAAC TATTTATGGA AATGGAATTA TGAGAGTGCC TCTCCCTGGA ACGAGCTGTT 
     2581 TGGCCTCAGC ATCAGTGACT CCCTTACCGA TGAACCTCCT GGATTCACTG ACGGTGCATG 
     2641 CCAAGATGAG CCTTATTCAC AGCATTGCAA CCAGAGTGAT AAAACTGGCT CATACGAAAT 
     2701 CCAGTGTTGC GCTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTACTG GTCTACATGG 
     2761 AAATTGAGTC TTTGGGCATC AAAGGATTTA TCAGTCAGCT CCTGCCCACT GTCTTCAAGT 
     2821 CCCACGCCTG GGGCATCCTG CACACACTGC TGGAGATGTT CAGCCACCGA ATGCACCACA 
     2881 TTCAGCCCCA CTACCGAGTT CAGCTCCTGA GCCATCTCCA CACACTGGCT GCAGTCGCAC 
     2941 AGACCAACCA GAACCAGCTC CATCTGTGTG TGGAGAGCAC TGCACTGAGG CTCATCACAG 
     3001 CCCTGGGAAG CTCAGAGGTC CAGCCGCAGT TCACGCGTTT CCTCAATGAT CCCAAAACAG 
     3061 TGCTGTCCGC CGAATCTGAA GAGCTGAACC GAGCCTTGAT CCTGACCTTA GCCAGAGCAA 
     3121 CCCACGTCAC AGATTTTTTT ACAGGATCTG ATTCAATACA GGGAACTTGG TGTAAAGATA 
     3181 TCCTTCAGAC CATCATGAAC TTTACTCCTC ATAACTGGGC TTCACATACC CTTAGCTGTT 
     3241 TTCCAGCCCC TCTGCAGGCT TTCTTCAAGC AAAATAACGT GCCCCAGGAA AGCCGTTTTA 
     3301 ATCTGAAGAA GAATGTGGAA GAAGAGTACA GGAAGTGGAA GTCAATGACT GATGAAAACG 
     3361 AGATCATCAC CCAGTTCTCT GTGCAGGGCT TCCCTCCACT CTTCCTCTGT CTCCTCTGGA 
     3421 AGATGCTCCT GGAGACGGAT CATATTAGTC AGATCGGCTA CAAGGTCCTG GAGAGGATTG 
     3481 GGGCCAGGGC CCTGGTGGCC CATGTGAGAA CATTTGCAGA TTTCCTGGTG TATGAGTTCT 
     3541 CTACGTCAGC AGGAGGTCAG CAACTCAATA AGTGCATTGA AATTCTCAAC GACATGGTGT 
     3601 GGAAGTACAA CATTGTCACG CTGGACAGAC TCATTCTCTG CCTGGCTATG CGTAGTCATG 
     3661 AAGGAAATGA AGCCCAGGTT TGTTATTTCA TAATTCAGTT GCTGTTGCTC AAACCAAATG 
     3721 ACTTCAGAAA CCGAGTAAGT GACTTTGTGA AGGAGAATTC CCCAGAGCAC TGGCTCCAGA 
     3781 GTGACTGGCA CACCAAGCAC ATGAGCTACC ACAAGAAATA TCCCGAGAAG CTGTATTTTG 
     3841 AAGGCCTGGC AGAGCAGGTT GACCCTCCTG TACCAATCCA GTCCCCTTAT CTGCCCATCT 
     3901 ACTTTGGAAA TGTGTGTCTC CGGTTCCTTC CAGTATTTGA TATAGTAATC CACAGGTTTT 
     3961 TAGAGTTGCT TCCAGTGTCC AAATCACTAG AGACTCTCCT GGATCACCTA GGGGGCTTAT 
     4021 ATAAATTCCA TGACCGGCCA GTGACTTATC TGTATAATAC TTTGCACTAT TATGAAATGT 
     4081 GCCTGAGAAA CCGTGACCAT CTCAAACGAA AACTTGTCCA TGCAATCATT GGCTCCCTCA 
     4141 AAGATAACCG GCCACAGGGC TGGTGTCTCA GTGATACCTA CCTGAAGCAT GCTATGAATG 
     4201 CACGGGAGGA CAACCCCTGG GTTCCGGAGG ACTCCTACTA CTGCAAGCTG ATTGGCCGAC 
     4261 TGGTAGACAC CATGGCGGGC AAGTCTCCAG GACCCTTCCC AAACTGTGAC TGGAGATTCA 
     4321 ATGAGTTTCC CAACCCCGCT GCCCACGCTC TGCATGTGAC GTGTGTGGAA CTCATGGCCT 
     4381 TGGCGGTTCC AGGCAAAGAT GTGGGGAATG CCCTTCTCAA TGTCGTCCTG AAAAGCCAGC 
     4441 CCTTAGTGCC AAGGGAGAAC ATTACAGCGT GGATGAATGC AATTGGTCTG ATCATCACGG 
     4501 CCCTCCCAGA GCCGTACTGG ATTGTGCTTC ATGACCGAAT TGTGAATGTG ATCAGCAGTT 
     4561 CTAGCCTGAC GTCAGAGACA GAGTGGGTCG GTTACCCGTT CCGCCTCTTC GACTTCACCG 
     4621 CTTGTCACCA GTCCTACTCG GAGATGAGCT GCAGCTATAC GTTAGCTCTA GCCCATGCCG 
     4681 TGTGGCACCA CTCCAGCATC GGGCAGCTCT CGCTTATCCC AAAGTTTCTC ACTGAGGCGC 
     4741 TTCTCCCGGT GGTGAAGACT GAGTTCCAGT TGCTCTACGT GTACCATCTT GTGGGGCCAT 
     4801 TTCTACAGAG GTTCCAACAG GAGAGAACCC GGTGCATGAT TGAGATTGGC GTGGCATTTT 
     4861 ATGACATGCT GTTGAATGTA GACCAGTGCA GCACGCACTT AAATTACATG GACCCCATCT 
     4921 GCGACTTCCT GTATCATATG AAGTATATGT TTACTGGTGA CAGTGTGAAA GAGCAAGTAG 
     4981 AGAAGATTAT CTGTAATTTA AAGCCGGCTT TAAAGCTTCG TCTTCGATTC ATCACGCACA 
     5041 TTAGCAAGAT GGAGCCGGCA GTGCCACCCC AGGCCCTCAA CAGCGGGTCT CCAGCACCTC 
     5101 AGTCTAACCA GGTGCCAGCA TCTCTGCCGG TGACTCAGAG CGGACCGACG CGTACGCGGC 
     5161 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5221 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5281 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5341 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5401 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5461 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5521 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5581 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5641 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5701 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5761 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5821 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5881 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5941 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6001 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6061 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6121 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6181 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6241 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6301 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6361 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6421 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6481 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6541 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6601 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6661 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6721 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6781 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6841 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6901 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6961 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7021 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7081 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7141 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7201 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7261 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7321 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7381 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7441 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7501 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7561 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7621 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7681 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7741 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7801 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7861 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7921 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7981 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8041 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8101 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8161 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8221 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8281 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8341 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8401 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8461 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8521 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8581 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8641 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8701 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8761 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8821 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8881 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8941 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//