LOCUS       MR211946                8961 bp    DNA     circular  SYN 
DEFINITION  Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 1
ACCESSION   NM_027804
VERSION     NM_027804.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5118..5209 
                     /label="Myc-DDK"  
     misc_feature    5239..5824 
                     /label="PolyA signal"  
     misc_feature    5296..5315 
                     /label="Restriction Enzyme"  
     misc_feature    6100..6772 
                     /label="ColE1"  
     misc_feature    7202..7996 
                     /label="Kan/Neo"  
     misc_feature    8031..8333 
                     /label="SV40 ori"  
     misc_feature    8419..8896 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5100..5106 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5100 
                     /label="Usp19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCAGGG GCCAGTGCTA CAGGGCCCAG GAGGGGGCCG CCAGGACTGG 
     1081 AAGAGGCCAC TAGTAAGAAG AAACAGAAGG ATCGAGCAAA CCTGGAAAGT AAAGATGGAG 
     1141 ATGCTAGGAG AGTGTCCCTT CCTCGAAAGG AACCAACCAA AGATGAATTG TTGCTCGATT 
     1201 GGAGGCAGAG TGCAGATGAG GTGATTGTTA AGCTGCGCGT GGGAACAGGT CCCGTACGTC 
     1261 TGGAGGATGT AGATGCTGCG TTCACAGACA CGGACTGTGT GGTGAGGCTT CCAGATGGTC 
     1321 GGCAGTGGGG TGGTGTCTTC TTTGCTGAAA TACAAAGTTC TTGCACCAAA GTGCAGGCTC 
     1381 GCAAGGGTGG TCTTCTACAG CTAGTACTAC CCAAGAAGGT GCCTCTGCTC ACGTGGCCCT 
     1441 CTCTCCTGAA ACCTCTGGGA ACCCAAGAGC TGGTGCCAGG TTTGCAGTGC CAGGAGAACG 
     1501 GGCAAGAGCT GTCTCCCATT GCCCTGGAGC CAGGCTCTGA GCCCCGCAGA GCTAAACAGG 
     1561 AAGCCCGAAA CCAGAAGCGG GCCCAGGGCC GTGGTGAGGT AGGCTCGGGG GCTGGCCCTG 
     1621 GGACACAGGC AGGGCCCAGC GCCAAGAGGG CTGTTCACCT CCGCAGAGGG CCAGAAGGGG 
     1681 AAGGGTCCAT GGATGGCCCC GGCCCCCAGG GTGATGCCCC GTCTTTCCTG TCTGACTCAG 
     1741 CTACCCAGGT TGAGGCTGAG GAGAAGCTCT GTGCTCCACC AATGAACACT CAAACAAGTC 
     1801 TCTTGAGCTC AGAGAAGAGT TTAGCCCTTC TGACAGTAGA GAAGACAGTG TCCCCCAGGA 
     1861 ATGACCCAGT CGCCCCGGTT ATGGTCCAGG ACAGAGACCC TGAGCCTGAG CAAGAAGACC 
     1921 AAGTCAAAGA GGAGATGGCA CTTGGGGCTG ATCCTACAGC CTTGGTGGAG GAACCAGAGT 
     1981 CTATGGTGAA CCTGGCATTT GTCAAGAACG ACTCGTATGA GAAGGGCCCG GATTCGGTGG 
     2041 TGGTGCACGT GTACGTGAAG GAGATCCGCA GGGACAGCTC CCGAGTGCTC TTCCGAGAGC 
     2101 AGGACTTCAC ACTGATCTTC CAGACCAGGG ACGGAAACTT TCTGAGGCTG CATCCGGGCT 
     2161 GTGGGCCCCA CACCATCTTC CGATGGCAGG TGAAGCTCAG AAACTTGATT GAACCAGAGC 
     2221 AGTGTACGTT CTGTTTCACG GCCTCTCGAA TCGATATCTG CCTCCGGAAG CGGCAGAGTC 
     2281 AGCGCTGGGG GGGACTGGAG GCCCCTGCTA CACGAGGTGC AGTGGGTGGT GCAAAGGTTG 
     2341 CCGTGCCGAC AGGCCCAACC CCTTTGGATT CAACCCCTCC AGGAGGTGGC CCCCACCCCC 
     2401 TGACAGGCCA GGAGGAAGCC AGGGCTGTGG AGAAGGAAAA ACCCAAGGCT CGATCAGAGG 
     2461 ACTCAGGGCT GGATGGTGTG GTGGCCCGCA CCCCCTTGGA GCATGTAGCC CCAAAGCCAG 
     2521 ACCCACACTT GGCCTCGCCC AAACCCACGT GTATGGTGCC TCCAATGCCG CACAGTCCAG 
     2581 TTAGTGGGGA TAGTGTGGAG GAGGACGAAG AGGAAGAGAA GAAGGTGTGC CTGCCAGGCT 
     2641 TCACTGGCCT TGTCAACTTA GGGAACACCT GCTTCATGAA TAGCGTCATT CAGTCTTTGT 
     2701 CCAACACTCG GGAACTTCGT GACTTCTTTC ACGACCGATC CTTTGAAGCT GAGATTAACT 
     2761 ACAATAACCC ATTGGGGACT GGTGGGCGCC TCGCCATTGG CTTTGCTGTG CTGCTCCGGG 
     2821 CCCTATGGAA GGGTACTCAC CAAGCCTTTC AGCCCTCCAA GCTAAAGGCC ATTGTGGCAA 
     2881 GCAAGGCCAG CCAGTTCACA GGCTATGCAC AGCATGATGC TCAAGAGTTC ATGGCTTTCT 
     2941 TGTTGGATGG GCTACATGAA GACCTCAATC GAATCCAAAA CAAACCCTAC ACAGAGACTG 
     3001 TGGACTCGGA CGGGCGGCCC GATGAGGTGG TAGCCGAGGA AGCATGGCAG CGGCACAAGA 
     3061 TGAGAAATGA TTCATTCATT GTGGACCTGT TTCAGGGCCA GTACAAGTCA AAGCTGGTGT 
     3121 GCCCTGTGTG TGCCAAGGTC TCCATCACTT TTGACCCGTT CCTTTATCTG CCGGTACCCT 
     3181 TGCCACAAAA GCAAAAGGTT CTCCCCATAT TTTATTTTGC CAGGGAGCCC CACAGCAAGC 
     3241 CCATCAAGTT CCTGGTGAGT GTCAGCAAGG AGAACTCCAG CGCGAGTGAA GTGTTGGACT 
     3301 CCCTCTCTCA GAGTGTCCAC GTGAAGCCTG AGAACCTGCG CCTAGCCGAG GTAATTAAGA 
     3361 ACCGTTTCCA CCGTGTTTTC TTGCCCTCCC ACTCACTGGA CGCTGTGTCC CCCACGGACG 
     3421 TGCTCCTCTG CTTTGAGCTG CTCTCCCCAG AACTGGCTAA GGAGCGGGTA GTAGTGCTGG 
     3481 AGGTGCAGCA GCGCCCCCAG GTACCCAGCA TCCCTATCTC CAAGTGCGCA GCCTGCCAGC 
     3541 GGAAGCAGCA ATCAGAAGAA GAAAAGCTGA AGCGCTGTAC CCGTTGCTAC CGTGTGGGCT 
     3601 ACTGCAACCA GTTCTGCCAG AAAACCCATT GGCCTGACCA CAAAGGCCTC TGCCGCCCTG 
     3661 AGAACATTGG CTACCCCTTC CTGGTCAGTG TGCCTGCTTC ACGCCTCACT TATGCCCGTC 
     3721 TTGCTCAGCT ACTAGAAGGT TATGCCCGGT ACTCTGTGAG TGTATTCCAA CCGCCCTTCC 
     3781 AGCCTGGCCG CATGGCTTTG GAATCGCAGA GCCCTGGCTG TACCACGTTG CTTTCAACCA 
     3841 GCTCTCTGGA GGCTGGGGAC AGTGAAAGAG AACCCATTCA GCCTTCTGAG CTCCAGCTGG 
     3901 TGACCCCTGT GGCTGAAGGG GATACAGGGG CTCACCGAGT ATGGCCGCCT GCTGATAGGG 
     3961 GTCCTGTGCC TAGCACCAGT GGACTCTCTT CTGAGATGCT GGCCAGTGGG CCTATCGAAG 
     4021 GTTGTCCCTT GCTTGCTGGT GAGAGGGTAT CTCGGCCTGA AGCTGCTGTG CCTGGGTACC 
     4081 AACACTCAAG TGAATCTGTG AATACCCACA CGCCCCAGTT CTTCATCTAT AAAATTGATG 
     4141 CATCAAACCG TGAGCAGCGG CTGGAGGACA AAGGGGAGAC ACCATTGGAG CTAGGTGATG 
     4201 ACTGTAGCCT GGCTCTGGTG TGGCGGAACA ATGAACGCCT GCAGGAGTTT GTGTTGGTAG 
     4261 CCTCCAAGGA GCTGGAATGT GCTGAAGATC CAGGCTCTGC TGGTGAGGCT GCCCGTGCTG 
     4321 GCCACTTCAC CCTGGACCAG TGCCTCAACC TCTTTACACG GCCTGAAGTG CTGGCACCTG 
     4381 AGGAGGCCTG GTACTGCCCA CAGTGCAAAC AGCATCGTGA GGCCTCCAAA CAGCTGCTGT 
     4441 TGTGGCGCCT ACCGAACGTG CTGATTGTGC AGCTCAAGCG CTTCTCCTTT CGTAGTTTCA 
     4501 TTTGGCGAGA CAAGATCAAT GACTTGGTGG AGTTTCCTGT TCGGAACCTG GACTTGAGCA 
     4561 AGTTCTGTAT CGGTCAGAAA GAGGAGCAGC TGCCTAGCTA TGACCTGTAT GCTGTCATCA 
     4621 ACCACTACGG AGGCATGATC GGTGGCCACT ATACTGCTTG TGCACGGCTG CCCAATGATC 
     4681 GCAGTAGCCA GCGCAGTGAC GTGGGCTGGC GCTTGTTTGA TGACAGCACG GTGACAACAG 
     4741 TAGACGAAAG CCAGGTCGTG ACGCGCTATG CCTATGTTCT CTTCTACCGT CGTCGGAACT 
     4801 CTCCTGTGGA GAGACCCCCC AGGGCAAGTC ACTCTGAACA CCACCCAGAC CTAGGCCCTG 
     4861 CAGCTGAGGC TGCTGCCAGC CAGGCTTCCC GGATTTGGCA GGAGCTCGAG GCCGAGGAGG 
     4921 AGATGGTACC TGAGGGGCCT GGGCCTCTGG GTCCTTGGGG GCCCCAAGAC TGGGTGGGGC 
     4981 CCCCGCCACG TGGCCCTACC ACACCAGACG AGGGTTGCCT CCGATACTTT GTCCTGGGTA 
     5041 CCGTGGCGGC TTTGGTGGCC CTTGTGCTCA ACGTATTCTA TCCTCTGGTA TCTCAGAGTC 
     5101 GCTGGAGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5161 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5221 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5281 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5341 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5401 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5461 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5521 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5581 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5641 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5701 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5761 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5821 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5881 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5941 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6001 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6061 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6121 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6181 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6241 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6301 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6361 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6421 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6481 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6541 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6601 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6661 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6721 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6781 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6841 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6901 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6961 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7021 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7081 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7141 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7201 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7261 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7321 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7381 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7441 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7501 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7561 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7621 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7681 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7741 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7801 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7861 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7921 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7981 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8041 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8101 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8161 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8341 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8401 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8461 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8521 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8581 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8641 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8701 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8761 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8821 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8881 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8941 CAAAAATTTA ACGCGAATTT T
//