LOCUS       MR211941                8937 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged) - Mouse synaptojanin 2 (cDNA clone MGC:77971 IMAGE:6838657)
ACCESSION   BC060214
VERSION     BC060214.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5092..5183 
                     /label="Myc-DDK"  
     misc_feature    5213..5798 
                     /label="PolyA signal"  
     misc_feature    5270..5289 
                     /label="Restriction Enzyme"  
     misc_feature    6074..6746 
                     /label="ColE1"  
     misc_feature    7176..7970 
                     /label="Kan/Neo"  
     misc_feature    8005..8307 
                     /label="SV40 ori"  
     misc_feature    8393..8870 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5067..5074 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5067 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGTGGGC AGAATTCCAG ATGCAGAGAT CTACAAAATC ACTGCCACTG 
     1081 AGTTGTACCC CCTGCAGGAA GAGGCCAAGG AAGAGGACCG CCTGCCCACC TTAAAGAAAA 
     1141 TCCTGAGCTC AGGGGTGTTC TATTTCGCAT GGCCCAATGA TGGCGCCTGC TTCGATCTGA 
     1201 CCATCAGGGC TCAGAAACAG GGTGATGACG GCTCTGAATG GGGGACCTCT TTCTTCTGGA 
     1261 ACCAGCTATT GCATGTGCCT CTGCGGCAGC ACCAGGTGAA CTGTCATAAC TGGTTGCTGA 
     1321 AAGTCATCTG TGGGGTGGTG ACCATCCGCA CAGTATATGC CTCCCACAAG CAGGCCAAGG 
     1381 CCTGTCTCAT CTCTCGCATC AGCTGTGAAC GCGCAGGTGC TCGCTTCCTC ACCCGTGGTG 
     1441 TGAACGATGA TGGCCACGTG TCCAACTTTG TGGAGACAGA GCAGACGATT TACATGGATG 
     1501 ATGGAGTATC GTCCTTTGTC CAGATCCGAG GCTCCGTTCC GCTGTTCTGG GAGCAACCAG 
     1561 GACTTCAGGT TGGCTCCCAT CATCTGAGAC TGCACAGAGG CCTAGAGGCC AACGCTCCTG 
     1621 CTTTTGAAAG GCACATGGTG CTTCTGAAGG AGCAATACGG TAAGCAGGTG GTTGTGAACC 
     1681 TGCTGGGTAG CAGAGGCGGT GAAGAGGTGC TCAACAGAGC CTTCAAGAAG TTGCTCTGGG 
     1741 CTTCTTGCCA CGCGGGTGAC ACACCTATGA TAAATTTTGA CTTCCATCAG TTTGCCAAAG 
     1801 GTAGGAAGCT AGAGAAATTG GAGAACCTGT TGAGACCTCA GTTACAGCTA CACTGGGAAG 
     1861 ACTTCGGCGT GTTTGCGAAG GGCGAGAATG TAAGTCCACG GTTTCAGAAA GGCACTCTGC 
     1921 GGATGAACTG TCTCGACTGT CTGGATAGAA CCAACACTGT GCAGTGCTTC ATTGCTCTTG 
     1981 AGGTCCTTCA TCTGCAGCTT GAGAGCTTGG GGCTAAATTC AAAGCCCATC ATTGACCGTT 
     2041 TTGTGGAGTC CTTCAAAGCC ATGTGGTCTC TGAATGGGCA CAGCCTGAGC AAGGTGTTCA 
     2101 CAGGGAGCAG GGCCCTGGAA GGAAAGGCCA AGGTGGGAAA GCTGAAGGAT GGGGCCCGAT 
     2161 CCATGTCTCG CACCATCCAG TCCAACTTCT TCGACGGGGT GAAGCAGGAG GCCATCAAGC 
     2221 TACTGCTAGT CGGAGATGTC TACAATGAAG AGTCTACAGA CAAAGGACGG ATGCTGCTGG 
     2281 ACAACACGGC CCTTCTGGCG ACCCCCAGGA TCTTGAAGGC CATGACAGAA CGCCAGTCGG 
     2341 AATTCACGAA TTTCAAGCGC ATCCAGATTG CTGTGGGGAC CTGGAATGTG AACGGAGGAA 
     2401 AGCAATTCCG TAGCAATCTC CTGGGGACGG CTGAGCTCAC GGACTGGCTC CTAGATGCTC 
     2461 CTCAGCTGTC AGGAGCAGTG GACTCCCAGG ATGATGGCAG TCCTGCTGAC GTATTTGCCA 
     2521 TCGGGTTTGA GGAGATGGTG GAACTGAGTG CGGGAAATAT TGTCAATGCC AGCACCACCA 
     2581 ACAGGAAGAT GTGGGGCGAG CAGCTTCAGA AAGCCATCTC CCGCTCCCAT CGGTACATCC 
     2641 TCTTGACCTC CGCACAGCTG GTGGGCGTCT GTCTTTACAT CTTTGTACGT CCGTACCACG 
     2701 TCCCGTTCAT CAGAGACGTG GCCATCGACA CCGTGAAGAC CGGCATGGGG GGAAAGGCGG 
     2761 GGAATAAGGG TGCTGTGGGC ATCCGCTTCC AGCTCCACAG CACCAGTTTC TGCTTCGTCT 
     2821 GTAGCCACCT GACGGCTGGG CAGTCTCAGG TGAAGGAGAG GAATGAAGAC TACCGGGAGA 
     2881 TCACGCACAA ACTCTCCTTC CCTTCGGGGA GAAACATATT TTCACATGAT TACGTGTTTT 
     2941 GGTGTGGCGA TTTCAACTAC CGTATTGATC TTACTTACGA AGAAGTCTTC TATTTTGTTA 
     3001 AACGCCAAGA CTGGAAGAAA CTTATGGAAT TCGATCAGTT ACAGTTACAG AAATCAAGTG 
     3061 GAAAAATTTT TAAAGACTTT CATGAAGGAG CCGTTAACTT CGGACCCACC TACAAGTATG 
     3121 ACGTTGGATC AGCTGCCTAC GACACAAGTG ACAAGTGCCG TACCCCAGCC TGGACAGACA 
     3181 GGGTGCTGTG GTGGAGGAAG AAGCATCCAT ATGATAAAAC AGCTGGTGAA CTCAACCTTC 
     3241 TAGACAGCGA TCTAGACGGC GATCCCCAAA TCAGACACAC CTGGTCTCCA GGCACTCTGA 
     3301 AATACTACGG CCGTGCAGAG CTGCAGGCGT CTGATCACAG ACCTGTGCTG GCCATTGTGG 
     3361 AGGTGGAGGT TCAAGAGGTG GATGTAGGAG CCCGGGAGAG GGTCTTCCAG GAAGTGTCCT 
     3421 CTGTCCAAGG CCCGCTGGAT GCCACCGTTG TTGTAAACCT CCAGTCTCCA ACCCTAGAAG 
     3481 AGAAAAATGA ATTTCCAGAG GACCTGCGCA CAGAACTTAT GCAGACTTTG GGGAATTATG 
     3541 GGACGATTAT TCTAGTCAGG ATCAACCAAG GGCAGATGCT GGTGACGTTT GCAGACAGCC 
     3601 ACTCGGCTCT CAGTGTGCTG GATGTGGATG GTATGAAGGT GAAAGGCAGG GCCGTGAAGA 
     3661 TTCGACCAAA GACCAAAGAT TGGCTAGAAG GCCTGAGAGA GGAGCTTCTC CGGAAGCGAG 
     3721 ACAGCATGGC CCCTGTGTCT CCCACCGCCA ACTCCTGCTT GTTGGAGGAG AACTTTGACT 
     3781 TCTCGAGTCT GGACTATGAG TCCGAAGGGG ATGTTCTTGA AGAGGATGAA GACTATTTAG 
     3841 TGGATGGGTT TGGCCAGCCT GTAGTCTCAG ACAGTGAGCT CGGTGGAGAC AACTCTTCCG 
     3901 ACACCATGAG CTCCTTGACA CCCGCCAGCA AGTCTCCCGC CCTGGCTAAA AAGAAGCAAC 
     3961 ATCCAACATA CAAAGCTGGC TTAATGGTGA AGAAGTCAGC CTCAGACGCG TCCATCTCTT 
     4021 CTGGCACTCA TGGACAATAT TCCATCTTGC AGACAGCGAA ACTTCTCCCA GGAGCACCCC 
     4081 AGCAACCACC CAAGGCTAGA ACTGGAATAA GTAAACCTTA CAACGTCAAA CAGATCAAAA 
     4141 CCACCAACGC TCAGGAGGCA GAAGCAGCTA TCCGGTGTCT CCTGGAAGCT AGCGGAGGGG 
     4201 TCCCGGAATC AGCCCCAGGT GCCATACCCC TGAGAAACCA AGGGTCTTCT AAGCCAGAGG 
     4261 CCACCCTGGG GCCCCCAGCC CTGCCCCGCC GGCCTGCTCC AAGGGTTCCC ACTATGAAGA 
     4321 AACCAACTTT GAGGAGGACA GGAAAGCCCA TGTTACCAGA AGAAAACTTT GAGCCACAGC 
     4381 CTGTCCATTT TACAATGGCT TCCCAGGAAA TGAACCTTGA GACCCCTCCT CCAATAACAG 
     4441 CTACTCCCAT CCCGCCTGTT CCCAAACCAA GAACACTTCA GCCTGGGAAA GGTGTTGAGG 
     4501 GGAGGCCAAG CAGCGGTAAG CCAGAACCAG ATGAAGCTCC TTCCGTGACA GGCACTGTGG 
     4561 AGTCACCACC TCCAGAGGCC CAGGAGGCCC CGTCTCTGGC TCCCAAGGTG CCTCCGAGGA 
     4621 GGAAGAAGTC TGCACCAGCG GCCTTCCACC TGCAGGTCCT GCAGAACAAC AGCCAAGTTC 
     4681 TCCAGGGCCT CACGTGCTCC AGCAGTTCTC CACCCTCACT GAAGCCCGAC ACCCACCCGC 
     4741 TCTGTCTACA AGTGGCACTT GGCACTTCAT CTGCCAGAAG CCCTGAAACC CATGGCCCCA 
     4801 GAGTGACAGA GCCCGAGGCG GCCTCTTTTC ATGGCAACTA TCCAGATCCC TTCTGGAGCC 
     4861 TTCTCCACCA CCCTAAGCTG TTGAACAATA CCTGGCTGTC CAAGAGCTCC GAGCCTTTAG 
     4921 ACGTGGGGTC CAGGAACCCT GAGAGGACAC ACACAGAGCC GGCACAGGTC AATGCATCGT 
     4981 TGGCTGAGAG GGGGCTTCCA CCAGACCATG GGGGAAAAGA CTTAAGTCAC TGGGTGACAG 
     5041 CTAGTAACAA AGACAAGAGG ACAACATTAG GTGTTAGCGG ACCGACGCGT ACGCGGCCGC 
     5101 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5161 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5221 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5281 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5341 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5401 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5461 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5521 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5581 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5641 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5701 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5761 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5821 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5881 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5941 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6001 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6061 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6121 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6181 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6241 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6301 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6361 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6421 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6481 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6541 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6601 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6661 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6721 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6781 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6841 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6901 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6961 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7021 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7081 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7141 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7201 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7261 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7321 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7381 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7441 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7501 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7561 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7621 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7681 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7741 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7801 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7861 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7921 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7981 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8041 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8101 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8161 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8281 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8341 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8401 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8461 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8521 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8581 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8641 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8701 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8761 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8821 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8881 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//