LOCUS       MR211939                8922 bp    DNA     circular  SYN 
DEFINITION  Gprasp1 (Myc-DDK-tagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 2
ACCESSION   NM_001004359
VERSION     NM_001004359.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5079..5170 
                     /label="Myc-DDK"  
     misc_feature    5200..5785 
                     /label="PolyA signal"  
     misc_feature    5257..5276 
                     /label="Restriction Enzyme"  
     misc_feature    6061..6733 
                     /label="ColE1"  
     misc_feature    7163..7957 
                     /label="Kan/Neo"  
     misc_feature    7992..8294 
                     /label="SV40 ori"  
     misc_feature    8380..8857 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5061..5067 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5061 
                     /label="Gprasp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTAGGGCA GAGGTTGAGC CTGGTGCCCA GGCAAAGGCT GAAAATAAGC 
     1081 CTGGGGATGA GAATGCTAAT GCTGCTGAAG TAGAGCCTGA AGCCCCATTG GTGGTCAGAC 
     1141 CCAAGGTTAG GACACAAATC ATGACTGGAG CAAGGCCCAA AGTGAAGCCT AAGGGTACCC 
     1201 CTGGAGCAAG ACCTAAAGGT GAAACCAGTA CACCAGGTGG GGCATATGCC AAATGTAAAC 
     1261 CTAAGGCAAT CCCTATTGCT CGATCCAAGC ATGATGCCCA AGTGTGGGCC CCAAATAAGT 
     1321 TTCGTGGCGA GTCCATGTCA AAGATGGGAA AGCAATGTCA GATCAGTGCT GCAGACCCTC 
     1381 CACTGCTCAG TAATGATTCT GGGATGGTTG CTCAAGCTAA GTGCCTGCCT GTAGATAGAG 
     1441 AACTTGCTAA TATGGACACT GAGAGTATTC CCAAAAAGGC TAATTCTCCA GCTGGATTCC 
     1501 AGCCTTCCTA TGGCTCAGAG GAGGGAACCA ATATGGGGTC CTGGTACCGT GCCAGGCCTG 
     1561 TCCCCAAAGG AGAGGCCTAT GAGAATTCCG ATTTCAAATG GGCAGATAAA CCCTCAGGAA 
     1621 GTCCCTCCTT TTGGAATAGA GATGAAGCCA GTACAAGATT TCGTCCTAGG AAAAGCATGA 
     1681 AGGCCAATAA CAGGTTCAGG CATATGGCCA AACAAGAGGC AAATACCATG CCTAGGCACA 
     1741 AAAACAAACA AGAGTTCTAT AATATTTCCA GTTCTGATTC TGAGGATGAG TCTGGTAAAA 
     1801 CTCCCTGGTT CTGGCCCAAA GACAAGACCA AAGTCTGGTC TAAGCCCAAA GAAGAGCCCA 
     1861 ATAGTAGGTC CTGGTTTAGG TCTAAGAAAG AAGTCCGTGT TGAATCCACT TCTGGGTCTG 
     1921 AATGTGAAAA TCCTACAAAA TCTTTGTTTT GGTCTGGAGA AGAGGCTAAA TCTAGATCCA 
     1981 AACCCAGAGC CAGGAAAGGG GTCAATATGA GGGCCAGGCA GCAGGCCAAG CGAGAAGCTT 
     2041 GCAGTGATGC CATGTCTGGA GCTATAGATA CAAATAAGAA AGAGTCCTGG TTCTTGCCTG 
     2101 AAGAGAAAGC TAATGTTTTT TCAAAGTCTA AGACAAAGAA AGAGCCCAGA ACCAGAGCAG 
     2161 TGCCAAAGGA AGAAGTCAAA ACTAAGGCCA GAGCAAGTAC CAAGCAAGAA GCCAGGCCAG 
     2221 AGGAGGAAGT CCTCGTTGGG GCTTGGTTCT GGGACACCCA GGAGTCCACT ATGGCAGATA 
     2281 GGATAAGCAT CAAGACCACT TTTGTAGAGG AGGAGCCCAT TGTTGGAGAT TGGTTCTGGA 
     2341 GTGAAGAAGA AGCCAGTGTG GACTCTGAGA CCTGTCATAC ATCCAGACCC AGGGCTAAGG 
     2401 AGGAGCAAGT TAGTAGTTTC TGTTTAGGGT CTGGAAAGAA GAGCAGTATG GAAAGTGGGC 
     2461 CAAAGGCTAC TTCTAAATCT ATGCCAGTAG CTAAGGAGGA TGAGGTCGTT ATTGGGTCCT 
     2521 GGTTCTGGGC AGATGATGAA GAGATCAACT TACAGGCTGA TGACGAGTCT ATTTTTGGAT 
     2581 CTTGGTTCTG GGGCACTGGT GAGAACAGCC TTAGATCTGT TGGAGTCAAT TGTGAGAAGA 
     2641 TGCCGAAGGC TGGAGAAAAA GAAGTTACTG ATTCCTGGTT CTGGGCTGGA GATGTCAATA 
     2701 CAGAGGCTGA GGTTGAAGAA CAGGCCAGGT CGGCATCTAC AAAAGCAACA ATCTTTGTGC 
     2761 CTTGGTTTTG GTCTGAAAAG CAGCCGAACA TGGATTTAGG GTCTGAACCT TGCTCAGATA 
     2821 TCATGGCAGG AGCTGAGGAG GAGCCCATAA TTGGGCCCTG GTTCTGGGCT AAAGTAGATA 
     2881 ATAGTGTGGA GGCTGAAGTT AACAGTAAGT CTAGCCTTGA GGATGAGGAA GAACCCATTA 
     2941 GATCCCCTTG GTTTGGGGCC AGAGAACAAA CCGATATGAA GTATGCAGCT GGTATCAGAT 
     3001 ACAAGCCTAT GGCAGAAGCT GAGGATGCTA ACAAAAAGTC TTGTGTCTGG GCAAAAGAAC 
     3061 CCTGTTTGTA TCCTACCAAT AGAGAATGCT TGAAATCTAC TCTGGGAGAA AAAGAAGACA 
     3121 CTGTTGATCC ATGGCTCTGG TCCAATAATT ATCCAAGGAC CAAGACCATC ACAGGGTCCT 
     3181 GGCTATGGGC TGCAGAAGAG GGAAATATAG ATGATGAGAC TGGAGAAAAG ATTAAACTAC 
     3241 CAACTTTAGA GGACAATGCA TTCAATTCTT GGTTCTGGAA AGAAAATGAA GAAAGCATTG 
     3301 TAGAGGCTCC CAAGAGAGAA GAATTCAGGC CAGAAGCGGA AGAGGAAGAC ATTATTGGTT 
     3361 CTTGGTTTTG GGCTGGGGAT GAAGACAGGT TTGAGCCAGC TGCTAAGATT AATGAAGAGA 
     3421 ACAAGATAGC ATCTGAAGAT GAAGATACAG TTGGATCCTG GTTCTGGGGC AATGAAGAGG 
     3481 CCAGTCTAGA GGCAGTGAGA AGGGGTACTT TTGAGTCTGC TCCTGGGATT AAGGAAGAGA 
     3541 AAGTTACTGG GTCATGGTTC TGGACTGATA AGGCCAAAGT AGGGGCTGGG TCCCAGACTG 
     3601 TCGAAACTGG GTCAGAAACT GAAGAGGAAG CAATTTTTGA GTCCTTGATC TGGGCTGCAA 
     3661 AAAAGGACAG TATTCAAGCA GGAGTAAAGC GTGTGTCCAA GCCAAAAGAT GACGGTAACA 
     3721 TAGCTGTTGG GTCCTGGCTC TGGTCTAGTG ACAAGGCCAC AAAAGAAGCT AAAACTCTGA 
     3781 TTGTCAGTGA GGCCAGTCCA GAAAATGGGA AAGAGTCAGT TGTTAAGTTT GGGTCAAGAG 
     3841 CAAAAGATGA GGTGATTAAC AAGACTGGCA GTGGTGACAA TTGTAAACAT AGTACAGAAG 
     3901 CTGAAACCAT AGTGGGAGCC TGGTTCTGGG AAGGAGATGA AGCTAGCTTT GAATCAAATC 
     3961 CTGTACCTGT ATGCAAGGCC GTTTGTGAAC CCGAGTCTTC AGCTGAGCAC GAACCTGACC 
     4021 CTTCCCGCAG GCCTCAGAGC TGGGATGAGG TCACTGTTCA GTTTAAGGCT GGTCCGTGGG 
     4081 GAAAAGCTGG CTTCCCACCC ATGAACCCCT TCAGATTCCC CAAAGAAGCG GCATCTCTGT 
     4141 TTGCAGAAAT GTTTGGGGGA AAGCCTAAGC TTGTGGAAGT TGGTCCAGAA AGGGAGCCTG 
     4201 AACCCCAGTT CCCATTTCAG TATGATCCCT CTTACCGCTC AGTCCGAGAA ATTCGCGAGC 
     4261 ATCTTAAGGC CAGGGAAAGT GCACAGCCAG AGAATTGGTC TTGCAACTGC ATCCAGTGTG 
     4321 AGCTTAGAAT TGGTTCTGAG GAGTTTGAGG AGCTTCTTTT ACTGATGGAC AGAAATCGTG 
     4381 ATCCTTTTAT CCATGAGATA TCTAAGATTG CAATGGGCAT GCGAGGTGCT TCTCAGTTTA 
     4441 CCCGGGATTT CATTCGAAAC TCGGGCGTTG TCTCCCTGAT TGAAGCCTTA CTTAACTATC 
     4501 CGTCCTCCCG AGTGAGGACA AGGTTTTTGG AAAATATGGT CCGCATGGCT CCCCCTTATC 
     4561 CAGACCTGAA CATGATTGAG ACATACGTGT GTCAGATTTG CGAGGACACC TTTGACTATG 
     4621 ACTTGGATTC CCCTGATCAG TTGTCCGGAT TGACAATGAT TACACACCTC ACTGCTACTT 
     4681 CTGACTATCA CAAGGTGGTT GTCAATTACT TGGCTGGGTT TTTCTACTTA CTGAATTCAG 
     4741 GCAATACCAA AACAAGGTTT CATGTTCTGA AACTGCTACT GAATTTGTCT GAAAACCTTG 
     4801 TCATGACAAA ACGCCTACTT GTTACTGATT CGGTGTCAGA ATTTATGGAC CTTATTAATA 
     4861 GGGAGGAGTC AGATGAAAAC ATTCAAATTG TTCTTGCAAT ATTTGAGACT ATCAGTAAAC 
     4921 ATATTCAAAA AGAGGCACTG TTTTCTGATG ATGATGATGA TGATGAAGAA GAAGATGCAG 
     4981 TGAATCTTGA ACCATTTATT TCTGCATTCC GTGAGGCTGA AAAAATTGCC AAGGAACTAA 
     5041 AACGCAAACC TGGCAATCAA AAAGCACCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5101 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5161 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5221 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5281 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5341 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5401 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5461 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5521 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5581 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5641 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5701 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5761 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5821 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5881 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5941 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6001 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6061 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6121 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6181 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6241 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6301 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6361 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6421 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6481 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6541 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6601 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6661 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6721 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6781 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6841 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6901 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6961 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7021 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7081 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7141 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7201 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7261 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7321 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7381 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7441 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7501 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7561 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7621 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7681 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7741 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7801 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7861 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7921 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7981 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8041 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8101 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8161 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8281 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8341 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8401 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8461 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8521 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8581 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8641 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8701 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8761 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8821 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8881 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//