LOCUS       MR211938                8922 bp    DNA     circular  SYN 
DEFINITION  Cenpj (Myc-DDK-tagged) - Mouse centromere protein J (Cenpj)
ACCESSION   NM_001014996
VERSION     NM_001014996.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5077..5168 
                     /label="Myc-DDK"  
     misc_feature    5198..5783 
                     /label="PolyA signal"  
     misc_feature    5255..5274 
                     /label="Restriction Enzyme"  
     misc_feature    6059..6731 
                     /label="ColE1"  
     misc_feature    7161..7955 
                     /label="Kan/Neo"  
     misc_feature    7990..8292 
                     /label="SV40 ori"  
     misc_feature    8378..8855 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5052..5059 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5052 
                     /label="Cenpj"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCTGATG CCAACCTCTT CAGAATTAAA CAGTGGGCAG AATTTCCTAA 
     1081 CCCAGTGGAT GACCAGTCCT TCTCGGGCTG GGGTCATACT AAATCGAGGA TTTCCTATTT 
     1141 TGGAAGCAGA TGATAAGCAA GCAGCTACAA ATGTTTCTAC GAGCTTCCCT GCTAAAGCCA 
     1201 CCCATTTTTC AAATAGCTTC AGTATTAGTA GTGAAGAGGA TTCATTTCAT GAAGAACAGA 
     1261 AGTTGGAGGC TGGAGGACCT TATAAACCAT GGTCAGAAAA CCCTGAAGCT CCTCCGGTCT 
     1321 TTCCTTCAGT TAGAAAGGAG CCAATTGCAT CACGTCAGGA TGCCCCGGGA TGCCAGGAGG 
     1381 ATAACAACAA TGATCTTACG CCTCATCTCG AGAGTGAATT CAAAGAAGTG GCTAATAAAA 
     1441 ACCCACTTTT TAAAAAACTG GAACAGCTGA AAGAGATCCA GCAGAAGAAG CAGGAGCAGC 
     1501 TGAAGAGGCA GCAGCTGGAA CAGCTGCAGA GGCTCATGGA GGAGCAAGAG AAGCTGCTCA 
     1561 CCATGGTGTC TGCACAGCAC GCGTTTCCAG GTACATTACT GCCTGATGAT CAGAGCCAGA 
     1621 AGCATCGCTC TCCCGGAGAT TTAACTCTGC CACCACACAG CTACTCAAAT CCAACCCAAG 
     1681 AAAACTCCTG TGCTTCCAAC GTTTTACCCG ATGAACAGAG TAATTTCTGT AGAGCCACTC 
     1741 AAGATTCCGT ATTAACTTCA AAAAATGCTT CGGATTTGTT TTACGAGTCA CAGTATCAAG 
     1801 AAGCACATGT GAAAAGAAAT GATTTAAAGG AGGAAAGTCC TGCCCACCCT TCAGGGGAAG 
     1861 GTGCTTTGCC ACGTTGGGAA AAGAAGATGG GGAGGAGTCA GGAAGGGAAG GATGTGAACT 
     1921 TACAAAAATG TGGAGATTCT TCAGAAGTGG TTAATATTGA TGAAAGGCCC ATCAAAGCTG 
     1981 CTGTTAGAGA GAAGCAGCAG ACATTTGAGG ACTACTTAGA AGAGCAGATC CAACTGGAAG 
     2041 AGCGGGAGCT TAGACAGAAG CAGCTGCAGG AAGCAGAAGG ACCATTGCTA GCGAAAACGA 
     2101 AACCCAAGCA ACCATTTTTA AAACGAGGAG AAGGTTTAGC TAGGTTTACT AATGCTAAAT 
     2161 CTAAATTTCA AAAGGGGAAA GAAAGTAAGC TAGCAAGTAC GCAGAGCCCA TCAGAGGACC 
     2221 AGCCTGGGTC TAAAGTAGAC AGACAGCATT TGCAGAGGAA AACTGCTCTC ATAAATAAAG 
     2281 ACCTGTGTGC AGAAACCCCC ACTGTTAAAA AGGACAGTAA AGCTAGACCC AAGGCTGGCT 
     2341 TTGCCTCACT CAGGCAGAAA CCCAAAGTGA CCAAGACCAA TATGAGAGAA AGTCTCTCCC 
     2401 CACCAGGACT GAAAGTCCAG ACGGGGAAGA AACGTGATGG ACAGTTTAGA CACCAGGTCA 
     2461 AAGGAGAACG AAATGCACAT GCTAGTAATA AGGAAAATGT GCCTGCATGC ATAAAGCCCT 
     2521 GGGATGCTGG TTGCAAGATG TGGAGTAAGA CACAGGGGAG AGAGAGACTT CCTCTTTCCA 
     2581 CTGGACCAGT TGGCTGTGTG GTCTCTAGGA GCCCGATACG TGAGACAGAC AGAGAAACAG 
     2641 AATCTTCCCT TGATTTTTCT CTTCAGAAAA AATTAGAGAT TTGGGAACGA GAAAAAGAAA 
     2701 AGGAAAACTT GGAATTAGAT GAATTTTTAT TTTTGGAACG GGCTGCTGAT GAAATATCAT 
     2761 TTTCTAGTAA TTCCTCATTT GTACTGAGAA TCTTAGAAAG GGACCAGCAG ATCTGCGACG 
     2821 GTCACAGGCT GTCTTCTACT CCTGTCAAAG CTGTACAGCA GCGTGAGGCA CAGCAGGCAG 
     2881 ATCCTAGGGG TCAGAGTAAC TGCAGTGAGA TTCCCCGTTA CGGTGTCGCA CATGAGAACG 
     2941 AGAGTGAATG CGAGGCCATG CTCTTGTCTT GGGGTTCAGG GTCACCTGAT GGTTTGAGGG 
     3001 AACTGTCCTG TAAACGTAGT ATGAAAGCCT TCCAAACCAG CACTTCTGAG ATCCAGAGCC 
     3061 AATGGGATGC AAGGGATGAT GGCGTTGCCA ACAGCGACAG TAGCACTGAG TCTGAGGAGC 
     3121 AGCATGACAT TACCATCAAA CCGTCCACCG AGGTTGGGGA CAGGGTCTTC AGCAACAGAG 
     3181 AGGATAGTCC ACAAGTCTGT GATGCCAAAG GACCTATTAG GGACACCGGG GCTCAGGAAG 
     3241 ATAAATGGAG AGACGCAGAC CTGGATCTGT CTGATAAAGA GTGTAGCAGT GACGAGTCTG 
     3301 TCATAGTTGA AAGCCTGAAC AATAAAGTAT TGGAACCACT GAGACTGCCC TCCTCTCAAG 
     3361 CTGGGAGTAA AATTGACTTC GATGATGAAA GATCGTGGAC AGACCTTGAG GAGAATCCAT 
     3421 ACGAACATGG TGTTATCCAC AGAGAGGAAG CCATATATGG TACACCCCAG ACACAGTGCC 
     3481 ACAGTAAGAG TGAAGGGTGT GTTCTGGACA AAACAATAAA AAGGAAGATT GCACCAGTCA 
     3541 AGAAGGGAGA AGACTTCAAG TGTGACAGGC GCATAAGCCC TCCTCCTCCA TCTGACTTGA 
     3601 TGGTAAAATT CTTCCCTTCT TTGAAACCAA AGCCAAAATT AGATTCACAC TTGGAAAATG 
     3661 AATCCAAGTT AAACCTGAGT CAAGATCAAC CACCAGAATT TATGGTCTGT TTTATAGGTG 
     3721 ACAGTGTTCG ATCTCAGGTT TTAAGAGAGA AAGTTACTGA ATTGGAATCA GAAATAGAAA 
     3781 AATTTAAAGC TGAGAACACA TCTTTAGCTA AACTTCGTAT TGAACGAGAA AGTGCCTTGG 
     3841 AAAAACTGAG GAAAGAAATT GCTGACTTTG AGCAACAAAA AGCAAGAGAG CTGGCTCGAA 
     3901 TAGAAGAGTA TAGAAAGGAG GAGACAAGGA AACTACAAAA GGAGCGCAAA GTCTTTGAGA 
     3961 AGTACACAGC AGCTGCAAGA ACATTCCCAG ATAAAAAGGA GCGCGAAGAA ATACAGGCCT 
     4021 TGAAACAGCA AATAGCAGAT TTACAGGAAG ACTTGAAAAG GAAGGAAACA AAGTGGTCAA 
     4081 GCACACAGAG TCGCCTCAGA AGCCAGATAG AGATGCTCGT CAAAGAGAAC ACAGACCTCA 
     4141 GGGAAGAGAT AAAAGTAATG GAAAGGTTCC GACTGGATGC CTGGAAGAGA GCAGAAGCTA 
     4201 TGGAGAACAG TCCCAAGGCC TGCCAGTACA TGATGGCCAC AAAAAAGGAT GAGTCCATGA 
     4261 ACTCATCATT TCAATTTCAA AAGAGTCATG TTTCTTCAGG AGTCCAGGTA GAAAAATATA 
     4321 AGAAAAAGTA TTTGCCAGCA CAAGGAAATC TATCTAGAAG AATCAAGTCT GCGCCTCCTC 
     4381 GTGATTTAGG CAGCTCCGAT AAAGGACAAG CTGCCTTGCC CAGAGAGCCT CTTCAACAAG 
     4441 TGAACTTCCC AGACCTTGAA TATAAAAACA AAGAGGAAAA AGAGGAAGAA ATTCAGGGAG 
     4501 AAATCAGTCA TCCTGATGGA AAGGTAGAAA AAATTTATAA AAATGGGCGC CGGGTTGTTC 
     4561 TGTTTCCCAA TGGAACTCGG AAAGAAGTGA GTGCGGATGG GAAGAGCGTC ACCGTTACAT 
     4621 TTTTCAATGG TGATGTGAAG CAGGTCATGC CAGATGAGAG AGTGGTCTAC TACTATGCTG 
     4681 CTGCCCAGAC CACACACACC ACGTACCCAG AAGGACTTGA AGTTTTACAT TTCTCAAGTG 
     4741 GACAAATAGA AAAGCATTTC CCAGATGGAA GAAAAGAAAT CACATTTCCT GACCAGACCA 
     4801 TTAAGACTTT ATTTGCTGAT GGTCAAGAAG AGAGCATCTT TCCAGATGGC ACAATTGTCC 
     4861 GAGTACAACG TGATGGCAAC AAGATCATAG AATTTAATAA TGGCCAAAGG GAGCTCCATA 
     4921 CTGCCCAGTT CAAGAGACGA GAGTATCCCG ATGGGACTGT TAAGACTGTC TATGCAAATG 
     4981 GTCATCAAGA GACAAAGTAC ACATCTGGCC GAGTTAGAGT GAAGGACAAA GACGGTAATG 
     5041 TGCTAATGGA CACAGAAATG AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5101 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5161 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5221 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5281 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5341 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5401 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5461 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5521 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5581 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5641 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5701 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5761 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5821 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5881 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5941 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6001 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6061 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6121 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6181 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6241 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6301 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6361 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6421 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6481 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6541 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6601 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6661 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6721 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6781 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6841 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6901 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6961 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7021 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7081 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7141 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7201 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7261 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7321 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7381 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7441 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7501 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7561 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7621 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7681 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7741 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7801 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7861 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7921 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7981 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8041 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8101 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8161 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8281 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8341 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8401 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8461 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8521 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8581 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8641 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8701 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8761 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8821 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8881 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//