LOCUS       MR211931                8883 bp    DNA     circular  SYN 
DEFINITION  Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893)
ACCESSION   BC052474
VERSION     BC052474
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5040..5131 
                     /label="Myc-DDK"  
     misc_feature    5161..5746 
                     /label="PolyA signal"  
     misc_feature    5218..5237 
                     /label="Restriction Enzyme"  
     misc_feature    6022..6694 
                     /label="ColE1"  
     misc_feature    7124..7918 
                     /label="Kan/Neo"  
     misc_feature    7953..8255 
                     /label="SV40 ori"  
     misc_feature    8341..8818 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5022..5028 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5022 
                     /label="Mink1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GCAGCCTGGA CGACATCGAC CTGTCTGCCC 
     1081 TGCGGGACCC TGCAGGAATC TTTGAGCTGG TGGAGGTGGT TGGCAATGGA ACCTATGGAC 
     1141 AGGTATACAA GGGGCGGCAC GTCAAGACTG GGCAGCTGGC TGCCATTAAG GTCATGGATG 
     1201 TCACAGAGGA TGAGGAGGAA GAGATCAAAC AGGAAATCAA CATGTTAAAG AAGTACTCTC 
     1261 ACCATCGCAA TATTGCCACC TACTATGGGG CCTTTATCAA GAAGAGCCCT CCTGGGAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GCGGTGCTGG TTCAGTGACC GACCTGGTAA 
     1381 AGAACACAAA AGGGAACGCA CTGAAGGAGG ATTGCATTGC TTACATCTGC AGGGAGATTC 
     1441 TCAGGGGTCT TGCCCATCTC CATGCCCACA AGGTGATCCA CAGAGATATC AAGGGACAAA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAAGTCA AGCTAGTGGA TTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC TGTGGGCAGG CGGAACACTT TCATTGGAAC CCCATACTGG ATGGCTCCAG 
     1621 AAGTCATTGC CTGTGACGAG AACCCCGATG CCACCTATGA CTACAGGAGT GACATTTGGT 
     1681 CTCTAGGAAT CACAGCCATT GAAATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CTATGCGGGC CCTCTTCCTC ATCCCTCGGA ACCCTCCCCC CAGGCTCAAG TCAAAGAAAT 
     1801 GGTCTAAGAA GTTCACTGAC TTCATCGACA CGTGTCTCAT CAAGACTTAC CTGAGCCGCC 
     1861 CACCCACCGA ACAGTTACTC AAATTCCCCT TCATCCGAGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTCAAGGAC CACATCGACC GCTCGCGGAA GAAGCGGGAG GAGACAGAGT 
     1981 ATGAGTACAG CGGCAGTGAG GAGGAAGACG ACAGCCATGG AGAGGAAGGC GAGCCAAGCT 
     2041 CCATCATGAA TGTGCCCGGT GAGTCCACAC TGCGCAGAGA ATTCCTCAGA CTCCAGCAGG 
     2101 AGAATAAGAG CAACTCTGAG GCTTTAAAGC AGCAGCAGCA GCTGCAGCAA CAGCAGCAGC 
     2161 GGGACCCGGA GGCACACATC AAACACCTGC TGCACCAGCG GCAGCGTCGC ATAGAGGAGC 
     2221 AGAAGGAGGA GCGGCGACGT GTGGAGGAGC AACAGCGGCG AGAGCGAGAA CAGCGTAAGC 
     2281 TACAAGAGAA GGAGCAGCAG CGGCGATTGG AAGACATGCA AGCCCTACGA CGAGAGGAAG 
     2341 AGAGGCGGCA AGCAGAGCGG GAACAGGAAT ACAAGCGGAA GCAGCTGGAG GAGCAGCGGC 
     2401 AGTCAGAGCG GCTGCAGAGA CAGCTGCAGC AGGAGCACGC CTACCTCAAG TCCCTGCAGC 
     2461 AGCAGCAGCA GCAGCAGCAG CTCCAGAAGC AGCAGCAGCA GCAGCAGCAG ATCCTGCCTG 
     2521 GAGACAGGAA GCCCCTGTAT CATTACGGTC GGGGCATTAA TCCTGCTGAC AAGCCAGCAT 
     2581 GGGCCCGCGA GGTGGAAGAG AGAGCACGGA TGAACAAGCA GCAGAACTCT CCCTTGGCGA 
     2641 AGGCGAAGCC AAGCAGTGCG GGGCCAGAGC CCCCCATCTC CCAGGCCTCT CCTAGCCCCC 
     2701 CAGGACCTCT TTCCCAGACT CCTCCTATGC AGAGGCCTGT GGAGCCCCAG GAAGGACCGC 
     2761 ACAAGAGCCT GGTGGCACAC CGGGTCCCAC TGAAGCCATA TGCAGCACCT GTACCCCGAT 
     2821 CCCAGTCCCT GCAGGACCAG CCGACTCGAA ACCTGGCTGC CTTCCCAGCC TCCCACGACC 
     2881 CTGACCCTGC TGCTGTCCCT ACACCCACTG CCACACCCAG TGCCCGAGGA GCTGTCATCC 
     2941 GCCAGAATTC AGACCCCACC TCTGAAGGGC CAGGGCCTAG CCCAAACCCT CCATCCTGGG 
     3001 TTCGGCCTGA TAATGAGGCT CCACCTAAGG TTCCACAGAG GACCTCTTCT ATCGCCACTG 
     3061 CCCTTAACAC CAGTGGGGCC GGAGGGTCCC GGCCAGCTCA GGCTGTCCGT GCCAGACCTC 
     3121 GCAGTAACTC CGCCTGGCAA ATCTATCTGC AGAGGCGGGC AGAGCGGGGC ACCCCCAAGC 
     3181 CTCCCGGGCC CCCAGCTCAG CCCCCTGGCC CGCCCAACGC CTCTAGTAAC CCTGACCTCA 
     3241 GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC CACGGCCACC 
     3301 TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC ACAAAACTGG 
     3361 ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC CGCTCAAGGC 
     3421 CAGGCCGGCC CGCAGACTTT GTGTTGCTCA AAGAGCGGAC TCTGGATGAG GCCCCTAAGC 
     3481 CTCCCAAGAA GGCCATGGAC TACTCCTCAT CCAGTGAGGA GGTGGAAAGC AGTGAAGAGG 
     3541 AGGAGGAGGA AGGCGATGGG GAGCCGTCAG AGGGGAGCAG AGACACTCCC GGGGGCCGCA 
     3601 GTGATGGTGA TACAGACAGC GTCAGCACCA TGGTGGTTCA TGATGTTGAG GAGATATCCG 
     3661 GGACCCAGCC CTCATATGGC GGCGGCACCA TGGTGGTCCA GCGTACTCCT GAAGAGGAAC 
     3721 GAAGCCTGCT GCTTGCTGAT AGCAATGGCT ACACAAACCT GCCTGATGTG GTCCAGCCCA 
     3781 GCCACTCACC TACTGAGAAC AGCAAAGGTC AAAGCCCTCC AACAAAGGAT GGAGGCAGTG 
     3841 ATTACCAGTC TCGTGGGCTG GTAAAGGCCC CAGGAAAGAG CTCATTCACC ATGTTTGTGG 
     3901 ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACACCAT CCCTATCACA GCCCTAGTGG 
     3961 GTGGAGAAGG TGGTCGCCTT GATCAACTGC AGTTCGATGT GAGGAAGGGC TCTGTGGTCA 
     4021 ACGTCAATCC CACCAACACC CGAGCTCATA GTGAAACTCC TGAAATTCGC AAGTACAAGA 
     4081 AGCGATTCAA CTCAGAGATC CTATGTGCAG CTCTCTGGGG GGTCAACCTC CTAGTGGGCA 
     4141 CAGAGAATGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT GGACTTATTG 
     4201 GGCGACGACG CTTCCAGCAA ATGGATGTCT TAGAAGGGCT CAACTTGCTC ATCACCATCT 
     4261 CAGGGAAAAG GAACAAACTG CGGGTATATT ACCTGTCCTG GCTTCGGAAC AAGATCCTAC 
     4321 ACAATGACCC AGAGGTGGAA AAGAAGCAGG GGTGGACCAC CGTGGGGGAC ATGGAGGGCT 
     4381 GCGGCCACTA CCGTGTTGTG AAATATGAAC GGATTAAGTT CCTGGTCATT GCCCTGAAGA 
     4441 ACTACGTGGA GGTTTATGCC TGGGCTCCCA AACCCTACCA CAAATTCATG GCCTTCAAGT 
     4501 CCTTTGCTGA CCTCCCTCAC CGCCCTCTAC TGGTGGACCT GACAGTAGAG GAGGGACAGC 
     4561 GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTT GATTCTGGGA 
     4621 ACAGCTATGA CATCTACATC CCTGTACATA TCCAGAGCCA GATCACACCC CACGCCATCA 
     4681 TCTTCCTCCC CAACACTGAT GGCATGGAGA TGCTGCTGTG CTATGAAGAT GAGGGTGTCT 
     4741 ATGTCAACAC TTACGGGCGG ATCATCAAGG ATGTGGTGCT GCAGTGGGGA GAGATGCCCA 
     4801 CCTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAG GCCATAGAGA 
     4861 TCCGCTCTGT GGAGACAGGC CACCTAGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC 
     4921 TCAAGTTCCT GTGTGAGCGC AATGACAAGG TGTTTTTTGC CTCTGTCCGC TCTGGAGGAA 
     4981 GCAGCCAAGT TTACTTTATG ACTCTGAACC GTAACTGCAT CATGAACTGG ACGCGTACGC 
     5041 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5101 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5161 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5221 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5281 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5341 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5401 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5461 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5521 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5581 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5641 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5701 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5761 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5821 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5881 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5941 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6001 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6061 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6121 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6181 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6241 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6301 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6361 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6421 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6481 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6541 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6601 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6661 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6721 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6781 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6841 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6901 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6961 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7021 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7081 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7141 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7201 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7261 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7321 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7381 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7441 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7501 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7561 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7621 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7681 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7741 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7801 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7861 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7921 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7981 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8041 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8101 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8161 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8221 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8281 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8341 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8401 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8461 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8521 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8581 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8641 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8701 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8761 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8821 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8881 TTT
//