LOCUS       MR211928                8850 bp    DNA     circular  SYN 
DEFINITION  Kdm3a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 2
ACCESSION   NM_001038695
VERSION     NM_001038695.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5007..5098 
                     /label="Myc-DDK"  
     misc_feature    5128..5713 
                     /label="PolyA signal"  
     misc_feature    5185..5204 
                     /label="Restriction Enzyme"  
     misc_feature    5989..6661 
                     /label="ColE1"  
     misc_feature    7091..7885 
                     /label="Kan/Neo"  
     misc_feature    7920..8222 
                     /label="SV40 ori"  
     misc_feature    8308..8785 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4995 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="Kdm3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTCACG CTCGGAGAAA GTTGGCCAGT ATTGGTGGGG AAGCGATTCC 
     1081 TCAGTCTGTC CGCAGCCGAA GGCAACGAAG GCGGCCAGGA CAACTGGGAC TTGGAGCGCG 
     1141 TTGCCGAGTG GCCCTGGCTG TCGGGGACCA TTCGAGCTGT TTCCCACACC GACGTTACTA 
     1201 AGAAAGACTT GAAGGTGTGT GTGGAGTTTG ATGGGGAGTC TTGGAGAAAG AGAAGATGGA 
     1261 TAGATGTCTA CAGCCTTCAG AGAAAAGCAT TTTTAGTAGA GCATAACCTG GTTTTGGCAG 
     1321 AACGAAAATC ACCTGAAGTT CCTGAGCAAG TTATTCAGTG GCCTGCAATA ATGTACAAAT 
     1381 CTCTTCTAGA CAAAGCTGGC TTGGGAGCCA TAACTTCTGT TCGGTTTCTT GGAGATCAAC 
     1441 AAAGTGTATT TGTTTCCAAA GACCTTTTGA AACCTATACA GGATGTTAAC AGTCTTCGGC 
     1501 TTTCCCTTAC TGATAATCAG ACAGTCAGTA AGGAATTTCA AGCTTTGATT GTAAAACATT 
     1561 TGGATGAAAG CCATCTTTTA CAAGGTGACA AGAACCTTGT TGGTTCAGAA GTAAAAATTT 
     1621 ATAGCTTGGA CCCATCTACT CAGTGGTTTT CAGCAACTGT TGTACATGGA AACCCATCAT 
     1681 CCAAAACTCT TCAAGTCAAC TGTGAGGAGA TTCCAGCACT GAAAATTGTC GACCCAGCAC 
     1741 TGATTCATGT TGAAGTTGTA CATGACAACT TTGTGACATG TGGTAATTCT ACAAGAACTG 
     1801 GAGCTGTAAA ACGCAAGTCT TCTGAGAATA ACGGAAGTTC GGTTTCTAAA CAAGCAAAAT 
     1861 CTTGTTCTGA GGCCTCTCCC AGTATGTGTC CTGTACAGTC TGTTCCCACA ACAGTGTTTA 
     1921 AGGAGATCCT GCTTGGCTGT ACTGCAGCAA CTCCATCTAG CAAGGACCCA AGACAGCAAA 
     1981 ATACTCCCCA GGCAGCCAAT TCTCCACCTA ACATTGGAGC AAAACTTCCT CAAGGATGTC 
     2041 ATAAGCAGAA CTTACCAGAA GAACTTTCTT CCTGTCTAAA CACAAAACCT GAAGTACCGA 
     2101 GAACAAAACC AGATGTCTGC AAAGAAGGAT TACTTTCTTC AAAATCTTCT CAGGTTGGAG 
     2161 CTGGAGACTT GAAAATTCTG AGTGAGCCCA AAGGTAGCTG TATCCAGCCT AAAACAAACA 
     2221 CTGATCAGGA GAGCAGACTG GAGTCTGCTC CACAGCCAGT CACTGGCCTT CCAAAGGAGT 
     2281 GCTTGCCTGC AAAGACTTCC TCTAAGGCAG AACTGGACAT TGCCACCACT CCTGAACTGC 
     2341 AGAAGCATCT AGAACATGCA GCTTCCACAT CCGATGACCT TTCAGATAAG CCAGAAGTGA 
     2401 AAGCAGGTGT CACTAGCCTT AATAGTTGTG CAGAAAAGAA GGTCGAACCT TCACATTTAG 
     2461 GTTCCCAGTC ACAGAATTTA AAGGAAACTT CAGTAAAAGT AGATAATGAA AGCTGTTGTA 
     2521 CAAGAAGCAG TAATAAAACC CAGACTCCCC CAGCCCGGAA GTCAGTTTTG ACAGACCCAG 
     2581 ATAAAGTCAG GAAGCTGCAG CAGAGCGGAG AGGCCTTTGT TCAGGATGAC TCCTGTGTTA 
     2641 ACATCGTGGC ACAGCTGCCC AAGTGTCGGG AGTGTCGACT AGACAGCCTG CGCAAGGATA 
     2701 AGGACCAGCA GAAGGACTCT CCTGTGTTTT GTCGCTTTTT CCACTTCAGG AGATTACAAT 
     2761 TCAACAAGCA TGGTGTGTTG CGGGTAGAAG GCTTCTTAAC ACCAAACAAG TATGACAGTG 
     2821 AAGCGATTGG CTTGTGGCTG CCTTTGACCA AAAATGTTGT GGGGACTGAT TTGGACACAG 
     2881 CAAAATATAT CCTGGCCAAT ATTGGAGACC ACTTCTGTCA AATGGTGATT TCTGAGAAGG 
     2941 AAGCTATGTC GACTATTGAG CCACACAGAC AGGTTGCTTG GAAACGAGCT GTCAAAGGAG 
     3001 TTAGAGAAAT GTGTGATGTG TGTGACACAA CCATTTTCAA CCTGCACTGG GTGTGCCCTC 
     3061 GGTGTGGGTT TGGAGTATGT GTAGATTGCT ACCGGATGAA GAGGAAGAAC TGCCAACAGG 
     3121 GTGCTGCCTA CAAGACTTTC TCTTGGATAA GGTGTGTGAA GAGTCAGATA CATGAGCCTG 
     3181 AGAACCTGAT GCCCACACAG ATTATTCCTG GCAAAGCCCT CTACGATGTT GGAGACATTG 
     3241 TGCATTCTGT CAGAGCAAAA TGGGGCATAA AGGCCAATTG TCCCTGCTCC AACAGGCAGT 
     3301 TCAAGCTCTT CTCAAAGCCA GCCTTAAAGG AAGACCTGAA ACAGACATCC TTGTCTGGAG 
     3361 AAAAACCAAC TCTTGGGACC ATGGTCCAGC AAAGTTCCCC TGTTTTGGAG CCAGTGGCTG 
     3421 TGTGCGGGGA AGCAGCCTCC AAGCCAGCCA GCAGCGTGAA GCCCACCTGT CCCACCAGCA 
     3481 CTTCACCTTT AAACTGGCTA GCTGACCTTA CCAGTGGGAA TGTCAACAAG GAGAATAAGG 
     3541 AAAAACAGCT GACTATGCCA ATTTTAAAGA ATGAAATCAA ATGCCTTCCA CCCTTGCCCC 
     3601 CTCTGAACAA GCCCAGCACA GTCCTCCATA CTTTTAACAG CACCATCTTG ACACCTGTGA 
     3661 GCAACAATAA TTCAGGTTTC CTTAGAAATC TTTTGAATTC ATCCACAGCA AAGACAGAAA 
     3721 ATGGATTGAA AAACACACCC AAAATTCTTG ATGACATCTT TGCCTCTTTG GTGCAAAACA 
     3781 AGACTTCTTC TGATTCATCC AAGAGGCCTC AAGGACTGAC AATCAAGCCT AGCATTCTTG 
     3841 GCTTTGACAC TCCTCACTAC TGGCTGTGTG ACAACCGCCT GCTGTGCTTG CAAGACCCCA 
     3901 ACAATAAGAG CAATTGGAAT GTTTTTAGGG AATGCTGGAA ACAAGGGCAG CCAGTGATGG 
     3961 TGTCGGGCGT GCATCATAAA TTAAACACTG AACTCTGGAA ACCCGAGTCC TTCAGAAAAG 
     4021 AGTTTGGTGA GCAGGAAGTA GACCTAGTCA ATTGTAGGAC CAATGAAATC ATCACTGGAG 
     4081 CCACAGTCGG AGACTTCTGG GATGGATTTG AAGATGTTCC AAACCGTTTG AAAAACGACA 
     4141 AAGAAAAAGA ACCAATGGTG TTGAAACTTA AGGACTGGCC GCCAGGAGAA GACTTCAGAG 
     4201 ACATGATGCC TTCCAGGTTT GATGATCTGA TGGCCAACAT TCCACTGCCT GAGTACACCA 
     4261 GGCGAGATGG CAAACTGAAC CTGGCTTCCA GACTGCCAAA CTACTTTGTA CGGCCAGACC 
     4321 TGGGCCCCAA GATGTACAAT GCTTATGGAT TGATCACTCC AGAGGATCGG AAATATGGGA 
     4381 CCACAAATCT TCACTTAGAT GTATCTGATG CAGCCAATGT CATGGTTTAT GTGGGAATTC 
     4441 CCAAAGGACA GTGTGAACAA GAAGAAGAAG TCCTTAGAAC CATCCAAGAT GGAGATTCTG 
     4501 ATGAACTCAC AATCAAGAGA TTTATTGAAG GAAAAGAGAA GCCAGGAGCC CTTTGGCACA 
     4561 TATATGCTGC TAAAGACACA GAGAAGATAA GAGAATTCCT TAAAAAGGTA TCAGAGGAGC 
     4621 AGGGTCAAGA CAACCCTGCA GACCATGACC CTATCCACGA TCAGAGCTGG TATTTAGACC 
     4681 GATCGCTGAG AAAGCGCCTC TATCAAGAGT ACGGCGTGCA AGGCTGGGCT ATTGTACAGT 
     4741 TTCTTGGGGA TGTGGTGTTT ATCCCAGCAG GAGCGCCACA TCAGGTTCAT AACTTATACA 
     4801 GCTGTATCAA AGTGGCTGAA GACTTTGTGT CTCCAGAGCA TGTTAAACAC TGCTTCTGGC 
     4861 TTACTCAGGA ATTCCGTTAC TTGTCACAGA CTCATACCAA CCATGAAGAT AAATTGCAGG 
     4921 TGAAAAATGT TATCTACCAT GCAGTGAAAG ATGCAGTTGC TATGCTGAAA GCCAGTGAAT 
     4981 CCAGTTTGGG CAAACCTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5041 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5101 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5161 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5221 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5281 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5341 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5401 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5461 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5521 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5581 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5641 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5701 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5761 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5821 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5881 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5941 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6001 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6061 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6121 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6181 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6241 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6301 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6361 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6421 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6481 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6541 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6601 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6661 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6721 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6781 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6841 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6901 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6961 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7021 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7081 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7141 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7201 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7261 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7321 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7381 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7441 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7501 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7561 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7621 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7681 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7741 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7801 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7861 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7921 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7981 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8041 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8101 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8281 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8341 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8401 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8461 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8521 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8581 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8641 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8701 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8761 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8821 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//