LOCUS       MR211925                8850 bp    DNA     circular  SYN 
DEFINITION  Pnpla6 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507)
ACCESSION   BC054789
VERSION     BC054789.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5007..5098 
                     /label="Myc-DDK"  
     misc_feature    5128..5713 
                     /label="PolyA signal"  
     misc_feature    5185..5204 
                     /label="Restriction Enzyme"  
     misc_feature    5989..6661 
                     /label="ColE1"  
     misc_feature    7091..7885 
                     /label="Kan/Neo"  
     misc_feature    7920..8222 
                     /label="SV40 ori"  
     misc_feature    8308..8785 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4995 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="Pnpla6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCCCA CTGCAAACCG GAATGGTGCT GGGCGTGATG ATCGGGGCCG 
     1081 GAGTGGCGGT GCTGGTCACC GCCGTGCTCA TCCTCTTGGT GGTGCGAAGG CTGCGGGTGC 
     1141 AGAAAACTCC AGCCCCGGAG GGTCCCCGGT ATCGGTTCCG GAAGCGGGAC AAAGTGCTTT 
     1201 TCTATGGCCG GAAGATTATG CGGAAGGTGT CACAGTCCAC TTCTTCCCTG GTGGACACAT 
     1261 CAGTCTCCAC CACTTCCCGG CCCCGCATGA AGAAGAAACT GAAGATGCTT AACATTGCCA 
     1321 AGAAGATCTT ACGTATCCAA AAGGAGACTC CGACCCTACA ACGGAAGGAG CCCCCACCTT 
     1381 CAGTGCTGGA GGCTGACCTC ACAGAGGGAG ACCTGGCCAA TTCCCACCTA CCCTCTGAGG 
     1441 TACTCTACAT GCTTAAGAAT GTTCGGGTGC TAGGTCACTT TGAGAAGCCG CTCTTCCTGG 
     1501 AGCTCTGCCG GCACATGGTC TTCCAGCGTC TCGGGCAGGG GGACTATGTT TTTCGGCCCG 
     1561 GTCAACCAGA TGCCAGCATC TATGTGGTTC AAGATGGGCT GCTGGAGCTC TGCCTACCAG 
     1621 GGCCTGATGG GAAGGAGTGT GTGGTGAAGG AGGTGGTTCC TGGAGACAGC GTCAACAGCC 
     1681 TTCTGAGCAT CCTTGATGTC ATCACGGGTC ACCAGCATCC CCAGCGGACT GTGTCAGCCA 
     1741 GGGCTGCACG GGACTCCACA GTGCTGAGAC TCCCAGTAGA AGCCTTCTCT GCTGTCTTCA 
     1801 CCAAGTACCC TGAGAGTTTG GTGCGAGTGG TACAGATCAT CATGGTAAGG CTGCAGAGAG 
     1861 TCACCTTCCT GGCGCTTCAC AATTACCTGG GTCTGACCAA TGAACTCTTC AGTCATGAGA 
     1921 TCCAGCCCCT ACGCCTTTTC CCCAGCCCCG GCCTCCCGAC CCGAACCAGC CCTGTCCGTG 
     1981 GCTCTAAACG GGTAGTCAGC ACCTCAGGTA CTGAGGACAC CTCAAAGGAG ACCTCTGGCC 
     2041 GGCCCCTGGA CTCCATTGGA GCTCCTCTGC CTGGACCTGC AGGGGATCCC GTGAAGCCCA 
     2101 CATCCTTAGA AGCACCCCCA GCCCCGTTAC TGAGCCGCTG CATCTCCATG CCAGTAGACA 
     2161 TCTCAGGCTT GCAAGGTGGC CCTCGTTCTG ATTTTGACAT GGCCTATGAA CGTGGACGGA 
     2221 TATCTGTTTC TCTTCAAGAA GAGGCATCTG GGGGACCTCA GACAGCATCC CCTAGGGAGC 
     2281 TCCGGGAGCA GCCAGCGGGT GCCTGTGAAT ATAGCTACTG TGAGGATGAG TCAGCCACAG 
     2341 GCGGATGTCC CTTTGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT GAGGCTGCAA 
     2401 AGAGAGAGCT AGCCAAGCTG ATGCGGATTG AGGACCCCTC TCTACTGAAC AGCAGAGTCT 
     2461 TGCTACATCA TGCCAAAGCC GGCACCATCA TAGCCCGCCA AGGGGACCAG GATGTGAGCC 
     2521 TGCACTTTGT GCTCTGGGGC TGCCTGCACG TGTACCAGCG CATGATTGAC AAGGCTGAGG 
     2581 AAGTGTGCCT ATTTGTGGCA CAGCCAGGGG AGCTGGTGGG GCAACTGGCG GTGCTCACTG 
     2641 GGGAACCTCT CATCTTCACA CTACGTGCCC AGAGAGACTG CACCTTCCTG CGGATCTCCA 
     2701 AATCCCACTT CTATGAGATC ATGCGTGCAC AACCCAGTGT GGTACTGAGT GCAGCTCACA 
     2761 CGGTGGCTGC TAGAATGTCC CCCTTCGTGC GCCAGATGGA CTTTGCCATT GACTGGACAG 
     2821 CTGTGGAGGC TGGTCGTGCG CTCTACAGGC AGGGAGACCG CTCCGACTGC ACCTACATTG 
     2881 TACTCAATGG ACGTCTCCGT AGTGTCATCC AACGAGGCAG TGGCAAGAAG GAGCTAGTTG 
     2941 GGGAGTATGG TCGAGGGGAT CTCATTGGCG TGGTGGAGGC ACTGACCCGG CAGCCACGAG 
     3001 CCACCACTGT ACACGCAGTG CGAGACACTG AGCTGGCCAA GCTTCCCGAG GGCACCTTAG 
     3061 GCCACATCAA ACGTCGGTAC CCACAGGTTG TGACCCGCCT TATTCATCTG CTAAGCCAGA 
     3121 AAATTCTAGG CAATCTGCAG CAGTTGCAAG GACCCTTTCC AGGCTCCGGG CTCAGTGTTC 
     3181 CGCAGCACTC GGAACTGACC AACCCAGCCA GCAATCTGTC TACTGTAGCT ATCCTCCCTG 
     3241 TGTGTGCTGA GGTGCCCATG ATGGCCTTTA CTCTGGAACT GCAGCATGCT CTGCAAGCTA 
     3301 TTGGTCCCAC GCTCCTCCTT AACAGTGACG TCATCCGGGC ACTCCTGGGG GCTTCAGCAC 
     3361 TGGATAGCAT TCAAGAATTC CGGCTGTCAG GGTGGTTGGC CCAGCAGGAA GATGCCCATC 
     3421 GCATTGTGCT CTACCAAACT GACACATCCC TGACGCCTTG GACCGTCCGT TGTCTGCGCC 
     3481 AGGCCGACTG CATCCTCATT GTGGGCCTGG GGGACCAGGA ACCCACTGTT GGCCAGCTGG 
     3541 AGCAAATGTT AGAGAACACT GCTGTCCGTG CCTTGAAGCA ACTGGTTTTG CTGCACCGGG 
     3601 AGGAAGGCCC TGGTCCCACG CGCACTGTAG AGTGGCTCAA CATGCGCAGC TGGTGCTCAG 
     3661 GGCACCTGCA CCTGCGCTGT CCTCGCCGCC TCTTCTCACG GCGCAGCCCA GCGAAACTTC 
     3721 ATGAGCTGTA TGAGAAGGTT TTCTCCAGGC GTGCAGACCG TCACAGCGAC TTCTCCCGCT 
     3781 TGGCACGAGT ACTCACAGGA AACACTATTG CTCTGGTGCT GGGTGGGGGC GGAGCCAGAG 
     3841 GCTGCTCGCA TATTGGGGTG CTGAAGGCAT TAGAGGAGGC AGGAGTCCCA GTCGACCTTG 
     3901 TGGGAGGCAC ATCCATAGGT TCCTTCATTG GGGCCTTGTA TGCCGAGGAA CGCAGCGCCA 
     3961 GCCGAACTAA ACAACGAGCC CGGGAGTGGG CCAAGAGCAT GACTTCTGTA CTGGAGCCTG 
     4021 TATTGGACCT CACATATCCT GTCACCTCCA TGTTTACTGG CTCAGCCTTT AACAGAAGTA 
     4081 TCCACCGTGT CTTCCAGGAT AAGCAGATTG AGGACCTGTG GCTGCCTTAC TTCAATGTGA 
     4141 CCACAGACAT CACTGCCTCT GCCATGCGCG TCCACAAAGA TGGCTCCCTG TGGCGGTATG 
     4201 TACGTGCCAG CATGACGCTC TCGGGCTACC TACCCCCGCT GTGCGACCCA AAGGATGGGC 
     4261 ACCTGCTCAT GGATGGTGGC TACATCAACA ACCTGCCAGC GGATATTGCC CGAAGCATGG 
     4321 GAGCCAAAAC GGTCATTGCC ATCGACGTTG GAAGCCAGGA TGAGACAGAT CTCAGCACCT 
     4381 ATGGGGACAG CCTGTCTGGC TGGTGGTTGC TGTGGAAAAG GCTAAACCCC TGGGCAGACA 
     4441 AGGTAAAGGT TCCAGACATG GCTGAGATCC AGTCCCGCTT GGCGTACGTG TCCTGTGTGC 
     4501 GGCAGCTAGA AGTCGTCAAG TCCAGCTCCT ATTGCGAGTA CCTTCGTCCA TCCATTGACT 
     4561 GCTTCAAGAC CATGGACTTT GGAAAGTTTG ACCAGATCTA TGATGTGGGT TACCAGTATG 
     4621 GGAAGGCTGT CTTTGGAGGC TGGACCCGTG GTGAAGTCAT TGAGAAAATG CTCACAGACC 
     4681 GGAGATCTAC AGACCTTAAT GAGAGTCGCC GTGCAGATAT ACTTGCCTTC CCAAGTTCTG 
     4741 GGTTCACTGA CTTGGCTGAG ATTGTGTCCC GGATCGAGCC ACCAACAAGC TACGTCTCTG 
     4801 ATGGCTGTGC TGATGGGGAG GAGTCGGATT GCTTGACAGA GTATGAGGAA GATGCAGGAC 
     4861 CAGACTGCTC AAGAGATGAA GGGGGCTCCC CTGAGGGTGC CAGCCCTAGC ACTGCCTCAG 
     4921 AGGTGGAAGA AGAGAAGTCC ACACTCCGGC AACGACGCTT TCTGCCTCAG GAGACTCCCA 
     4981 GCTCAGTCGC AGATGCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5041 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5101 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5161 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5221 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5281 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5341 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5401 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5461 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5521 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5581 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5641 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5701 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5761 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5821 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5881 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5941 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6001 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6061 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6121 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6181 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6241 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6301 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6361 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6421 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6481 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6541 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6601 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6661 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6721 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6781 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6841 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6901 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6961 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7021 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7081 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7141 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7201 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7261 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7321 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7381 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7441 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7501 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7561 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7621 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7681 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7741 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7801 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7861 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7921 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7981 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8041 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8101 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8281 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8341 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8401 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8461 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8521 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8581 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8641 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8701 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8761 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8821 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//