LOCUS       MR211921                8841 bp    DNA     circular  SYN 
DEFINITION  Rnf123 (Myc-DDK-tagged) - Mouse ring finger protein 123 (cDNA clone MGC:67486 IMAGE:5704926)
ACCESSION   BC057082
VERSION     BC057082.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="Rnf123"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCCAAG GGGACTGGCA TGTCGTTCTC CCGAAAGAGC TATAGGCTGA 
     1081 CCTCAGATGC TGAGAAGTCC AGGGTCACAG GCATCGTGCA AGAGAAACTA CTGAGCGACT 
     1141 ATCTGTACCG CATCTTTTCC CCTCCTGACC GTGGACCCGC CGCAGCCACC AGCAGGAAAC 
     1201 CGCTAAACTT CCATAACCTG CCTGAGCACG TGGACCAGCT GCTACAGGTG GACAGTGAAG 
     1261 ACAACGAGAG CCAGGGACAA GTTGAAGGTC GACTTGGCCC ATCTACTGTG GTCCTAGACC 
     1321 ACACAGGAGG CTTTGAGGGG CTTCTCCTTG TGGATGATGA CCTCCTGGGG GTGATTGGAC 
     1381 ACAGCAACTT TGGCACTATC CGTTCTACCA CATGTGTGTA CAAAGGGAAG TGGGTCTACG 
     1441 AGGTGCTCAT CTCCTCCCAG GGCCTCATGC AGATCGGCTG GTGCACCATC AACTGCCGCT 
     1501 TTAATCAGGA GGAAGGGGTT GGAGACACAC ATAACTCCTA TGCCTATGAC GGCAACCGAG 
     1561 TGCGCAAGTG GAATGTTACC ACCACGAATT ATGGCAAGGC GTGGGCTGCG GGGGACATTG 
     1621 TCAGCTGCCT AATTGATCTG GATGATGGGA CTCTGTCCTT CTGCCTGAAT GGCGTGTCAC 
     1681 TGGGCACTGC CTTCGAGAAC CTTTCCAGGG GCCTAGGAAT GGCGTACTTC CCAGCCATCA 
     1741 GCCTGTCATT CAAGGAGTCT GTGGCATTCA ACTTTGGCAG CCGTCCTTTG CGCTACCCAG 
     1801 TTGCGGGCTT CCGGCCCCTG CAGGACCCTC CGTTTGCTGA CCTGGTCCGG GCACAGAGGT 
     1861 TGCTGGGCTG CTTCCAGGCA GTGCTAAGTG TGGAGCTGGA CCCTGTGGAA GGGCGGCTGG 
     1921 TGGAGACGGA GAGCTCTGAG TGGCAGCTGC AAGGGCAGCC CACTGTCCTC CTCACGCTGG 
     1981 CCCACATCTT CCATCACTTT GCACCACTGC TGCGCAAGGT ATACCTGGTG GAGGCTGTGC 
     2041 TAATGAGCTT CCTGCTGGGC GTTGTGGAGA AGGGCACACC AGAGCAGGCG CAGTCTGTGG 
     2101 TACACCAGAT CTTGGACCTC TTGTGGCTCT TCATGGAGGA CTATGAGGTA CAGGATTGCC 
     2161 TGAAGCAGTT GATGATGTCA CTTCTACGTC TCTACCGATT CTCGCCTATT GTCCCAGACC 
     2221 TGGGTCTACA GATCCACTAC CTGCGCCTCA CTATGTCCAT CCTGAGACAC GAGAAGTCCC 
     2281 GCAAGTTCCT GCTTAGCAAT GTCCTTTTTG ACATGCTCCG GTCCGTGGTC TTCTTTTATA 
     2341 TTAAGAGTCC CCTGCGTGTG GAGGAAGCTG GCCTGAAGGA ACTCATTCCC ACCACCTGGT 
     2401 GGCCCCATCG CTCCAGCAGG GAGAGCAGAG ACGGTAAGGA AGCAAGGGAG GAGACCACCG 
     2461 AAGAGCGGCA GCGGAGGCGA GCCTATGAGC GTGGCTGCCA AAGACTCAAG AAACGCATTG 
     2521 AAGTGGTGGA AGAACTGCAG GTCCAGATCC TGAAGCTGCT GTTGGACAAT AAAGATGACA 
     2581 ATGGGGGTGA AGCTTCTAGG TACATCTTTC TGACAAAATT CCGAAAGTTC CTGCAGGAGA 
     2641 ATGCCAGCGG CCGGGGGAAC ACACCCGTGC TCTGCCCCCC TGAGTACATG GTCTGCTTCC 
     2701 TACACCGGCT GGTGTCTGCC TTGCGCTTCT ATTGGGATGA ATACAAAGCT TCCAACCCCC 
     2761 GTGCTTCCTT CAGTGAGGAG GCTTACATCC CGCCCCAGAT CTTCTATAAT GGCAAGGTGG 
     2821 ACTACTTTGA CCTTCAGCGC CTTGGGGGCC TCCTCTCACA CCTTCGAAAG ACCCTTAAAG 
     2881 ATGACCTTGC TTCCAAAGCC AACATCGTGA TCGACCCCCT GGAGCTCCAG GCAGCCACCA 
     2941 TGGATGACCT GGATGAGGAT GAAGAGCCTG CCCCCTCAGC GGCCCAGGTG TGGCAGGAGG 
     3001 GACAGCGTCC GATGCAAGCC CTGGCCATCG GAGGGGCACT GCCCCTGCCC CGGCCAGGCT 
     3061 GGCTCAGTTC TCCAACCCTG GGCAGAGCCA ACCGCTTCCT CAGCACGGCA GCTGTGAGCC 
     3121 TCATGACCCC ACGGCGGCTT CTGAGCACCA TGGAGAAAGT CAAAGTTCGC TCACTGAATG 
     3181 TGGAACAGAG GACCCGTGAG GACATTGAGG GCAGCCACTG GAATGAGGGC CTGCTGTTGG 
     3241 GGAGGCCCCC TGAAGAGCCT GAGCAGCCGC TTACCGAGAA CTCGCTGTTG GAAGTCCTGG 
     3301 ATGGCACAGT CATGATGTAT AACCTCAGCG TTCACCAGCA GCTGGGCAAG ATGGTGGGTG 
     3361 TGTCTGATGA TGTCAACGAG TATGCAATGG CCCTAAGAGA CACAGAGGAC AAGCTCCGTC 
     3421 GGTGCCCTAA GAGGAGGAAG GATATCCTTG CAGAGTTGAC CAAGAGCCAG AAGGTTTTCT 
     3481 CAGAAAAGCT GGACCACCTG AGCCGCAGGC TTGCCTGGGT CCACGCCACA GTCTACTCAC 
     3541 AGGAGAAAAT GCTGGATATC TACTGGTTAC TGCGTGTCTG CCTACGGACC ATTGAGCATG 
     3601 GGGACCGCAC GGGGTCTCTC TTTGCCTTCA TGCCTGAGTT CTACCTAAGT GTGGCTATCA 
     3661 ACAGCTACAG TGCCCTGAAG AACTATTTTG GCCCTGTGCA CAGCATGGAG GAACTCCCAG 
     3721 GCTATGAAGA GACCCTGACA CGCTTAGCTG CCATCCTCGC CAAACACTTT GCTGACCCTC 
     3781 GAATAGTAGG CACTGATATT CGAGACTCAC TGATGCAGGC CCTGGCCAGC TATGTGTGCT 
     3841 ACCCACACTC CCTGCGGGCT GTGGAACGGA TTCCTGAGGA ACAGCGCATC GCCATGGTGA 
     3901 GGAACCTTTT GGCACCCTAT GAGCAACGGC CCTGGGCCCA GACCAACTGG ATCCTGGTGC 
     3961 GGCTTTGGAG GGGCTGTGGG TTTGGGTACC GCTATACACG GCTGCCACAT CTGCTGAAAA 
     4021 CCAAGCCAGA GGATGCCAAT TTGCCCAGCC TCCAAAAGCC CTGCCCTTCG ACCTTGCTAC 
     4081 AGCAGCACAT GGCGGACCTG CTGCGACAAG GGTCTGATGT GGCACCGAGC TTCCTCAACA 
     4141 GTGTCCTTAA CCAGCTCAAC TGGGCCTTCT CTGAGTTCAT CGGCATGATC CAGGAGATTC 
     4201 AACAGGCTGC TGAACGCCTG GAGCGGAACT TTGTGGACAG CCGACAGCTC AAGGTCTGTG 
     4261 CCACCTGCTT TGACCTGTCG GTCAGCTTGT TGCGCGTCTT GGAAATGACC ATCACGCTGG 
     4321 TACCTGAAAT ATTCCTTGAC TGGTCCCGCC CTACCTCTGA GATGCTGCTT CGGCGTCTGG 
     4381 CACAGCTGCT GAACCAGGTG CTGAACCGGG TGACAGCTGA GAGGAACCTG TTTGACCGTG 
     4441 TAGTTACCCT ACGGCTACCT GGGCTGGAGA GTGTGGACCA CTACCCTATC CTGGTGGCAG 
     4501 TGACTGGCAT CCTGGTACGC CTCCTGGTGC ACGGCCCAAC CTCAGAGACA GAGCAAGCCA 
     4561 CCTCTGTGCT CCTGGCTGAT CCCTGCTTCC AGCTTCGTTC CATATGCTAT CTCCTGGGGC 
     4621 AGCCAGAGCC CCTAGCACCT GGCACTACCT TGCCTGCCCC TGACCGGAAA CGCTTCTCTC 
     4681 TACAGAGTTA TACAGATTAT ATCAGCGCTG AGGAGCTGGC CCAGGTGGAA CAGATGCTGG 
     4741 CTCACCTGAC CGCTGCATCT GCCCAGGCGG CCGCCGCCTC CCTGCCCACC AATGAAGAGG 
     4801 ACCTCTGCCC AATCTGCTAC GCCCACCCCA TCTCTGCTGT GTTCCAGCCT TGTGGTCACA 
     4861 AATCCTGCAA AGCCTGCATC AACCAGCACC TGATGAACAA CAAGGACTGC TTCTTCTGCA 
     4921 AAGCCACCAT TGTATCTGTA GAGGACTGGG ACAAGGCAGC CAACACAAGC GCCATGTCCT 
     4981 CAGCTGCCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//