LOCUS       MR211920                8949 bp    DNA     circular  SYN 
DEFINITION  Kif24 (Myc-DDK-tagged) - Mouse kinesin family member 24 (Kif24)
ACCESSION   NM_024241
VERSION     NM_024241.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5106..5197 
                     /label="Myc-DDK"  
     misc_feature    5227..5812 
                     /label="PolyA signal"  
     misc_feature    5284..5303 
                     /label="Restriction Enzyme"  
     misc_feature    6088..6760 
                     /label="ColE1"  
     misc_feature    7190..7984 
                     /label="Kan/Neo"  
     misc_feature    8019..8321 
                     /label="SV40 ori"  
     misc_feature    8407..8884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5088..5094 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5088 
                     /label="Kif24"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCCTGG TTATATGAGT GTCTTTGTGA GGCTGAGCTT GCACAGTATT 
     1081 ATCCTCATTT TACTGCGCTT GGCCTTCAAA AAATAGATGA ATTAGCCAAG GTTACAATGA 
     1141 AGGACTACTC CAGATTGGGA GTCCATGACA TGAACGACCG CAAACGTCTC TTTCAGCTTA 
     1201 TCAAAATCAT TAAGATTATG CAGGAGGAAG ATAAAGCCCT CGGTATCCCA GAGCATCCTC 
     1261 TTCAGGCAAG CAGCCTGTAC ACCAAGCCTC GGGAATTCAG ATCTGGGCCC CGAAGACAAC 
     1321 TGCATTTTGA TTCTCCTTCT GCCAGCAAAG ATAAAATGGC CAACAATGAA ACAGGCAGTT 
     1381 TATCCAACTT CTCTGTGGAT GAGCAGAAGT CCACTTACCT GAAAGTGCTG GAGCACATGC 
     1441 TACCAGATGA TTCCCAGTGC CAGACAAAAA TAAGAGCCCC GGATGCCTCT GCTGCTGATG 
     1501 CTTCCATGCA AACGGAAACC AACGCTCCGC TCTTTTCATC AAATTACTTC TCTCCACAAC 
     1561 TGGGAAATTG TGATATTCCC GTTATTCAAA GAGTCTCTCA TGTGTCAGGG TATAACTATG 
     1621 GAATTCCTCA TTCTTGTGTC AGGCAGATCA CCTCAGAGAA TCCTTGGACT GAAATGGAGA 
     1681 AAATCAGAGT TTGTGTTCGA AAACGGCCTT TAGGTGTAAG GGAAGTACGT CGTGGAGAAG 
     1741 TTAATGTTAT TACTGTAGAA GATAAAGAAA CTCTGCTCGT ACATGAGAAA AAAGAAGCAG 
     1801 TTGACCTTAC TCAATATATT CTTCAGCATG TTTTTTATTT TGATGAAGTC TTTGGTGAGG 
     1861 CATGCAGCAA TCAAGATGTA TACCTGAAGA CTGCTCACCC CCTCATTCAG CATATTTTCA 
     1921 ACGGAGGAAG TGCCACTTGC TTTGCATATG GACAAACAGG TGCGGGGAAA ACCTATACCA 
     1981 TGATAGGAAC TCATCAAAAC CCAGGATTGT ATGCTCTGGC TGCCAAAGAT ATCTTCAGGC 
     2041 AGCTGAAAGT ATCCCAGTCA AGAAGGAATC TCTTTGTGTG GATCAGCTTC TATGAGATCT 
     2101 ACTGTGGACA ACTTTATGAC CTCCTAAATA GAAGAAAACG GCTCTTTGCA AGAGAAGATA 
     2161 GCAAGCATGT GGTACAGATA GCTGGCCTTC GAGAGCTCCA AGTGGACAGT GTGGAGCTAC 
     2221 TCCTACAGGT GATCTTAAAG GGCAGCAAGG AGCGCAGCAC AGGGGCCACT GGTGTCAACG 
     2281 CAGACTCCTC CCGTTCCCAT GCTATCATCC AAATTCAGAT CAAAGATTCA GCCAAGAGGA 
     2341 CATTTGGCAG GATCTCTTTC ATTGACTTGG CTGGGAGTGA GAGAGCAGCA GATGCCAGGG 
     2401 ACTCAGACAG ACAGACAAAG ATGGAAGGCG CGGAGATCAA CCAGAGTCTT CTGGCTCTGA 
     2461 AAGAATGTAT CCGAGCACTG GACCAGGAAC ACACCCACAC GCCTTTCAGG CAGAGCAAAC 
     2521 TGACTCAGGT CCTGAAGGAC TCTTTCATTG GCAATGCCAA AACTTGCATG ATCGCCAACA 
     2581 TCTCCCCAAG CCACATAGCC ACAGAGCATA CATTGAATAC CTTGCGCTAT GCTGACCGGG 
     2641 TGAAGGAACT AAAGAAAGGC GTTAAGTGTT GTGCTTCAGC TACCAGTCAG AATCAGACAT 
     2701 CTGCAAACGC CTCTCCAAAA CGAATTCAGA GCTCCCCTGT CACCCTCCCC GGGGACAAGT 
     2761 GCTCCCCCAA AAAAGTTAAG CTGGGACTTC AGCAGTCACT TACTGTGGCC CCAGGCCCCA 
     2821 CAAAAGTTAA AGCCCATCCT TTGGCCAGCC ATGTTCCCAA CGTCCCCTTT ACCTCTGGAC 
     2881 CTAAAACCCC TGGTAAAAAA AGTAGTTCCC GAGGGAGTCC CACTCCAGAG TGGGACATGA 
     2941 AGGCTAGCCC TCGCAAAGGA ACTACGAGAT CTGGCCATTC GATAAAAAAG GGAGCAGAGT 
     3001 CGGCACCACT GTGCTCTGAG AAAAGTCAAA TTGGCAGCAA GATTGCTGTT GGGTGGGAGG 
     3061 GCAGGGCCTC AGACCCAGGA GAAGGTCTGC TGCGTGTCAG GCTGCCCACG AGAGGGAAGA 
     3121 AGGTGCAGCC AGTCCAGCCA GTGCAGAAGC AGCTCCTGTC CCGACCTCGG CTCCTTGCTA 
     3181 ACAGCCACCA CTTAGAAGCC ACTCAGGACA GCAAGGTGGG CACACCTGCC GGGCTTGCCC 
     3241 CCGAAGCCTG GACAAACCCC ATTCTACAGC AAAAGGAAAG GGAGGAGCAT TTGCGATTCT 
     3301 ACCACCAGCA GTTCCAGCAG CCACCGCTCC TCAAGCAGAA GCTAAATTAC CAACCCCTGC 
     3361 AGAGGCTTTT ATGCCAGCAC AGGCCTTCAG AAGGTCGGCT CCAGAGTGAG ACTGGCTTTC 
     3421 CCCTCCACTC TAACCCTGAG AACCGTGATG GAGCCCAGGC TGAGGACCTT GATGACAGTG 
     3481 ACTTCAGTGA GGATTCTTTC TCGCACGGCT CCAGTCAGCC GGCCATGAAA CAGGGGAGCA 
     3541 CAGCCTTGGA AAGGAGCGGA AGTTCCTTCT TCCTTCATCA GGACAGGGAG CACAGCCCTG 
     3601 AGGAGCAGGC AGCAGAAAGG CAGCAATGTT TGCTTTTCAG CTCTGAGACG GATGGCAGTA 
     3661 AGAAGCGGCC AGCTGACAGC TGGGTGTATT CCAGGGACCC CATCATCAGC CACAGAAGAG 
     3721 GAGCACTCAG TCAAAGCCAC AGCCCAAGTA TGGTATGCCC AGACTGGAGC AAGGAGGAAG 
     3781 ACTCTGCCTC CTCAGGACCT TCTCCTAAGG ACAACCGGGC CCAGAAACCT TCCTCTTCAC 
     3841 AGGTAGATTT TGTGCATCAC CAAAAACCGG GTGAAGCTCA AGTCTCTGAC ATCAGACTGG 
     3901 AGGCCTTCAC AAGTGAGGTT CCTGAGCAAG CTGAGGGCAG CTTGTCATCC CCATCCCCGG 
     3961 AAAATGGGCT GTCTTTTCCA CTGTCCCATG TGGCTGTTTC TGGATCCCCA GACCAAAGAG 
     4021 ACAGAGTCTG CACACCATTA AGAGAAGTCA GTGAAAACAG AGTGACTCAC ACTCCAGGAA 
     4081 GAGTGAACAG CAGCACTCCT TTCCAAGAGG ACTCTGGAGA GCAGATACAG ATGTGTTCTG 
     4141 CAAATGCTTC TGGACTCATG GCTCCCCTCA CTATGTCCCT CCTGGAGACC CCCTGTCATG 
     4201 AGGACCTTTC GTCCTTGGAG CAAATTGCCC AGGATGGAGC TGGCTATGGT TTCATGGCTG 
     4261 AGATTGTAGG AGGGCCAGCA GCAGGACACA CAGTCCCATC TTATGATCAA GAGGCTGCTT 
     4321 TGCCAGTGTC TTCAGCTACT GAGTGCCTAT GGCTATCTTC ATCTCCCCCT GACAATCGGC 
     4381 CTAGTGGTGA TCTTCCAGCT CTGTCCCCGT CACCCATCCA CCAGCATTCA CCTGACAAAT 
     4441 TGCCCGGTAG AGAGGCCTAC CAGACCAGAA GGCCAATACT CTTGCCTGAG AATCATATGG 
     4501 GTAGTAAGCT ATATGATGAC CGTGCTGAAG AGACTGAACT GGGGGGCTCC CTCACATTCC 
     4561 CCAGAAAGCC GTCCTCCAAC ATACATGCCG GAGTACCCTA TTCTACACCT TTCCTCACCT 
     4621 CATGTACAGG AAGTAGTAAT GGAGTTGGCC GTCCCTGGGC CCAGGAGAGA AAACATCCTA 
     4681 CAGGGGTTAG CTGCCAGGAG CTTGTTTCCT CTACAGATTC CAACAAGCCT CATTACAACG 
     4741 AGGACATCGC ATGGCTCAGG CACAGGCCAA TCTCAAGGTG CTTAGATTCA GACTCTCCTG 
     4801 TGGTCCCCAG CTGTTCTTCC AAGGCCTTGA GGACATATTG TCCACTTACA CCGGAGCAGG 
     4861 CACAGCAGGT GATTATCCGT GCACACAAAG AACAGCTGGA TGAAATGGCT GAGCTGGACT 
     4921 TGAAGGAGGA GACCTTAATG ACCCAGATGG ATTCTAATGA TTTTGAAGAT TTTGTGACCC 
     4981 AGCTGGATGA AATCATGGCT TTGAAGTCCA GGTGCATCCA AAGTCTGAGA AGCCAGCTAC 
     5041 AGCTCTACCT CACCAGCCAC AGGCCCGCTG CAGCCCCCGA AAGAACTGTG GTGTCTACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//