LOCUS       MR211918                8835 bp    DNA     circular  SYN 
DEFINITION  Col18a1 (Myc-DDK-tagged) - Mouse collagen, type XVIII, alpha 1 (Col18a1), transcript variant 2
ACCESSION   NM_009929
VERSION     NM_009929.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4990..5081 
                     /label="Myc-DDK"  
     misc_feature    5111..5696 
                     /label="PolyA signal"  
     misc_feature    5168..5187 
                     /label="Restriction Enzyme"  
     misc_feature    5972..6644 
                     /label="ColE1"  
     misc_feature    7074..7868 
                     /label="Kan/Neo"  
     misc_feature    7903..8205 
                     /label="SV40 ori"  
     misc_feature    8291..8768 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4965..4972 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4965 
                     /label="Col18a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCAGG TGGCACCTCC TGGATGTGCT CACCAGTTTG GTCTTGCTGC 
     1081 TGGTGGCACG CGTCTCCTGG GCAGAGCCAG AGAATGTTGC TGAGGAGGTG GGGCTGCTGC 
     1141 AGCTCCTTGG AGACCCCCTA CCTGAGAAGA TCTCACAAAT CGATGACCCT CACGTCGGGC 
     1201 CGGCCTACAT CTTTGGACCA GACTCCAACA GTGGCCAGGT GGCCCAGTAT CATTTCCCAA 
     1261 AACTCTTCTT CCGGGACTTT TCGCTGCTGT TTCATGTCCG GCCAGCCACA GAGGCAGCAG 
     1321 GGGTGCTATT TGCCATCACA GATGCTGCCC AGGTGGTAGT CTCACTGGGC GTGAAGCTCT 
     1381 CAGAGGTCCG AGATGGACAG CAAAACATCT CATTGCTCTA CACGGAGCCT GGGGCCAGCC 
     1441 AGACCCAGAC GGGAGCCAGC TTCCGCCTAC CTGCATTTGT TGGGCAGTGG ACACACTTCG 
     1501 CGCTCAGCGT CGACGGAGGC TCTGTGGCTC TCTACGTAGA CTGTGAAGAA TTCCAGAGGG 
     1561 TGCCATTTGC TCGGGCCTCG CAGGGACTGG AGCTAGAGCG TGGCGCTGGC CTCTTTGTGG 
     1621 GTCAGGCTGG AACAGCAGAC CCTGACAAGT TCCAGGGGAT GATCTCAGAG CTGAAGGTAC 
     1681 GCAAAACCCC CCGGGTGAGC CCTGTGCACT GTCTGGATGA AGAAGATGAT GATGAAGACC 
     1741 GGGCATCTGG AGATTTTGGA AGTGGCTTTG AAGAAAGCAG CAAGTCACAC AAGGAGGATA 
     1801 CATCTCTACT ACCTGGGCTC CCTCAGCCAC CTCCTGTCAC TTCCCCACCC CTGGCTGGAG 
     1861 GCAGCACCAC AGAAGATCCT AGAACAGAAG AAACGGAGGA AGACGCCGCG GTAGATTCTA 
     1921 TAGGAGCTGA GACCCTTCCT GGCACAGGTT CAAGCGGTGC ATGGGATGAG GCTATCCAGA 
     1981 ACCCCGGAAG GGGCTTGATA AAGGGAGGTA TGAAAGGACA AAAGGGAGAA CCAGGTGCCC 
     2041 AGGGCCCACC TGGCCCAGCT GGGCCCCAGG GTCCTGCCGG TCCAGTGGTC CAGAGCCCCA 
     2101 ACTCACAACC TGTCCCTGGA GCACAAGGAC CCCCGGGACC TCAGGGGCCA CCAGGGAAGG 
     2161 ATGGCACTCC AGGAAGGGAT GGTGAACCGG GTGACCCTGG TGAAGATGGG AGACCGGGTG 
     2221 ACACTGGACC TCAAGGCTTT CCAGGGACCC CAGGAGATGT GGGCCCTAAG GGCGAGAAGG 
     2281 GAGATCCTGG TATTGGGCCC CGAGGACCTC CAGGGCCTCC AGGGCCACCA GGACCCTCCT 
     2341 TCAGACAAGA CAAGCTGACC TTCATTGACA TGGAGGGATC CGGTTTCAGC GGAGACATAG 
     2401 AGAGCCTTAG AGGCCCACGA GGCTTCCCTG GCCCCCCGGG GCCCCCTGGT GTCCCAGGAC 
     2461 TTCCTGGTGA GCCAGGACGC TTTGGGATCA ATGGTTCCTA TGCACCAGGA CCTGCAGGCC 
     2521 TTCCTGGTGT ACCTGGGAAG GAAGGACCCC CCGGTTTTCC AGGTCCCCCG GGACCTCCAG 
     2581 GTCCTCCAGG CAAAGAGGGC CCACCAGGAG TGGCCGGCCA GAAAGGCAGT GTTGGTGATG 
     2641 TGGGCATCCC AGGACCCAAG GGGAGCAAAG GAGACCTTGG GCCCATCGGT ATGCCTGGCA 
     2701 AGTCTGGCTT GGCTGGATCC CCTGGGCCAG TTGGACCCCC AGGACCTCCA GGGCCTCCAG 
     2761 GGCCACCAGG ACCAGGATTT GCTGCTGGAT TCGATGATAT GGAAGGCTCT GGAATACCCC 
     2821 TCTGGACAAC AGCCCGAAGC TCTGATGGGC TGCAGGGACC TCCCGGGTCG CCGGGACTCA 
     2881 AGGGGGATCC TGGAGTGGCA GGCCTACCTG GAGCCAAGGG AGAAGTTGGA GCAGATGGAG 
     2941 CCCAGGGCAT CCCTGGTCCC CCAGGAAGAG AAGGTGCAGC TGGATCTCCG GGGCCAAAAG 
     3001 GAGAGAAAGG GATGCCGGGA GAAAAGGGAA ACCCAGGAAA AGATGGAGTG GGCCGGCCGG 
     3061 GCCTCCCTGG GCCTCCAGGA CCTCCAGGGC CTGTGATCTA TGTGTCAAGT GAGGATAAAG 
     3121 CAATAGTGAG CACGCCAGGA CCTGAGGGCA AGCCAGGGTA CGCAGGCTTT CCTGGACCTG 
     3181 CTGGACCGAA GGGTGACCTG GGTTCCAAAG GCGAGCAGGG TCTTCCGGGG CCCAAGGGTG 
     3241 AGAAGGGAGA GCCAGGCACT ATCTTTAGTC CTGATGGCAG AGCTCTGGGC CATCCCCAGA 
     3301 AGGGAGCCAA GGGAGAGCCA GGCTTTCGAG GACCCCCGGG TCCTTATGGA CGACCTGGGC 
     3361 ACAAGGGTGA AATTGGCTTC CCTGGACGGC CGGGTCGACC TGGAACGAAT GGCTTAAAGG 
     3421 GAGAGAAAGG AGAGCCTGGA GATGCCAGCC TTGGGTTCAG CATGAGGGGA TTGCCTGGCC 
     3481 CCCCTGGGCC TCCAGGACCC CCAGGTCCTC CTGGGATGCC CATCTATGAC AGCAATGCAT 
     3541 TTGTGGAGTC TGGCCGACCT GGACTACCAG GACAGCAGGG TGTGCAGGGG CCTTCAGGAC 
     3601 CAAAGGGTGA CAAAGGAGAG GTGGGCCCAC CTGGGCCACC AGGGCAATTC CCCATTGACC 
     3661 TCTTCCACCT GGAAGCGGAA ATGAAGGGGG ACAAGGGAGA CCGAGGGGAT GCTGGACAGA 
     3721 AAGGAGAGAG GGGAGAACCT GGGGCTCCTG GTGGTGGATT CTTCAGCTCA AGTGTACCTG 
     3781 GCCCCCCCGG CCCACCTGGA TACCCTGGAA TTCCGGGTCC AAAGGGAGAG AGCATCCGGG 
     3841 GGCCACCTGG CCCTCCTGGC CCGCAGGGAC CTCCTGGCAT TGGCTATGAG GGTCGCCAGG 
     3901 GTCCCCCAGG ACCTCCGGGA CCTCCAGGAC CTCCCTCCTT CCCTGGCCCT CACAGACAGA 
     3961 CTGTCAGTGT TCCTGGTCCT CCGGGCCCAC CTGGTCCTCC AGGTCCCCCA GGAGCCATGG 
     4021 GTGCCTCTGC TGGGCAGGTG AGGATCTGGG CCACATACCA GACCATGCTG GACAAGATCC 
     4081 GGGAGGTGCC GGAGGGCTGG CTCATCTTTG TGGCCGAGAG GGAAGAGCTC TATGTACGCG 
     4141 TTAGAAATGG CTTCCGGAAG GTGCTGCTGG AGGCCCGGAC AGCCCTCCCG AGAGGCACGG 
     4201 GCAATGAGGT GGCTGCTTTG CAGCCCCCAT TGGTCCAGCT TCATGAGGGC AGTCCATACA 
     4261 CCCGGAGGGA GTACTCCTAT TCCACGGCAC GACCCTGGCG AGCAGATGAC ATCCTGGCCA 
     4321 ACCCACCGCG CCTGCCAGAC CGCCAGCCTT ACCCCGGAGT TCCACATCAC CACAGTTCCT 
     4381 ATGTGCACCT GCCGCCAGCC CGCCCCACCC TCTCACTTGC TCATACTCAT CAGGACTTTC 
     4441 AGCCAGTGCT CCACCTGGTG GCACTGAACA CCCCCCTGTC TGGAGGCATG CGTGGTATCC 
     4501 GTGGAGCAGA TTTCCAGTGC TTCCAGCAAG CCCGAGCCGT GGGGCTGTCG GGCACCTTCC 
     4561 GGGCTTTCCT GTCCTCTAGG CTGCAGGATC TCTATAGCAT CGTGCGCCGT GCTGACCGGG 
     4621 GGTCTGTGCC CATCGTCAAC CTGAAGGACG AGGTGCTATC TCCCAGCTGG GACTCCCTGT 
     4681 TTTCTGGCTC CCAGGGTCAA CTGCAACCCG GGGCCCGCAT CTTTTCTTTT GACGGCAGAG 
     4741 ATGTCCTGAG ACACCCAGCC TGGCCGCAGA AGAGCGTATG GCACGGCTCG GACCCCAGTG 
     4801 GGCGGAGGCT GATGGAGAGT TACTGTGAGA CATGGCGAAC TGAAACTACT GGGGCTACAG 
     4861 GTCAGGCCTC CTCCCTGCTG TCAGGCAGGC TCCTGGAACA GAAAGCTGCG AGCTGCCACA 
     4921 ACAGCTACAT CGTCCTGTGC ATTGAGAATA GCTTCATGAC CTCTTTCTCC AAAAGCGGAC 
     4981 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5041 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5101 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5161 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5221 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5281 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5341 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5401 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5461 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5521 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5581 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5641 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5701 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5761 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5821 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5881 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5941 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6001 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6061 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6121 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6181 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6241 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6301 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6361 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6421 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6481 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6541 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6601 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6661 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6721 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6781 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6841 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6901 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6961 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7021 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7081 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7141 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7201 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7261 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7321 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7381 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7441 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7501 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7561 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7621 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7681 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7741 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7801 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7861 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7921 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7981 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8041 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8101 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8161 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8221 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8281 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8341 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8401 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8461 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8521 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8581 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8641 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8701 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8761 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8821 TTTAACGCGA ATTTT
//