LOCUS       MR211913                8814 bp    DNA     circular  SYN 
DEFINITION  Zfp521 (Myc-DDK-tagged) - Mouse zinc finger protein 521 (Zfp521)
ACCESSION   NM_145492
VERSION     NM_145492.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4971..5062 
                     /label="Myc-DDK"  
     misc_feature    5092..5677 
                     /label="PolyA signal"  
     misc_feature    5149..5168 
                     /label="Restriction Enzyme"  
     misc_feature    5953..6625 
                     /label="ColE1"  
     misc_feature    7055..7849 
                     /label="Kan/Neo"  
     misc_feature    7884..8186 
                     /label="SV40 ori"  
     misc_feature    8272..8749 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4959 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="Zfp521"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCGCCGC AAGCAAGCGA AACCGAGATC CCTCAAAGAC CCCAACTGTA 
     1081 AACTTGAAGA CAAGATTGAA GATGGGGAGG CAGTAGACTG CAAGAAGAGG CCAGAAGACG 
     1141 GGGAGGAGTT GGAGGAGGAC GCTGTGCACA GCTGTGACAG CTGCCTCCAG GTGTTTGAGT 
     1201 CACTGAGCGA CATCACAGAG CACAAGATCC ATCAGTGCCA GCTGACAGAT GGCGTTGACG 
     1261 TTGAGGACGA CCCCAGCTGC TCTTGGCCAG CCTCCTCGCC TTCCAGTAAG GACCAGACTT 
     1321 CCCCCAGCCA TGGAGAAGGG TGTGATTTCG GAGAGGAAGA AGGTGGCCCT GGACTGCCTT 
     1381 ACCCATGCCA GTTCTGTGAC AAGTCCTTTA GCCGCCTCAG CTACCTAAAG CACCATGAAC 
     1441 AGAGCCACAG TGACAAGCTG CCCTTCAAGT GCACCTACTG CAGTCGGCTG TTCAAACACA 
     1501 AGCGGAGCCG TGACCGCCAC ATAAAGCTCC ACACTGGGGA TAAGAAGTAT CACTGTAGCG 
     1561 AGTGTGACGC AGCCTTCTCC CGGAGCGATC ATCTCAAGAT CCACTTAAAG ACTCACACCT 
     1621 CCAACAAGCC ATATAAATGT GCCGTCTGCC GCAGAGGGTT CCTCTCCTCT AGCTCCTTGC 
     1681 ACGGACATAT GCAGGTTCAT GAGCGGAACA AGGACGGCTC CCAGTCTGGC TCCAGGATGG 
     1741 AGGACTGGAA GATGAAGGAC ACTCAGAAGT GCAGCCAGTG CGAGGAAGGC TTTGACTTCC 
     1801 CGGAAGACCT CCAGAAGCAC ATTGCAGAAT GCCACCCCGA GTGCTCCCCT AATGAGGACC 
     1861 GAGCAGCCCT CCAGTGCATG TACTGCCACG AGCTGTTTGT GGAGGAGACG TCCCTCATGA 
     1921 ACCACATCGA GCAGGTACAC GGGGGTGAGA AGAAGAACTC TTGCAGCATT TGCTCAGAGA 
     1981 GCTTCCTCAC GGTCGAGGAG CTGTATAGCC ACATGGACAG TCACCAGCAG CCAGAGTCCT 
     2041 GCAATCACAG CAACAGCCCT TCCCTGGTCA CTGTGGGTTA CACCTCAGTG TCCAGCACGA 
     2101 CTCCAGACTC GAACCTCTCA GTGGACAGCT CGACCATGGT AGAGGCTGCA CCACCGATTC 
     2161 CAAAGAGCCG CGGGAGGAAG CGAGCTGCTC AGCAGACCTC CGATATGACT GGCCCCTCTA 
     2221 GTAAGCAGGC GAAAGTCACC TACAGCTGTA TTTACTGCAA CAAGCAGTTA TTTTCCAGTC 
     2281 TCGCGGTTCT GCAGATTCAC TTGAAAACTA TGCATTTAGA TAAGCCTGAG CAGGCTCATA 
     2341 TCTGTCAGTA TTGCTTGGAG GTCTTACCCT CACTCTATAA CCTAAATGAA CATCTTAAAC 
     2401 AAGTGCACGA AGCTCAGGAC CCCGGCCTGA TTGTTTCGGC CATGCCTGCC ATTGTTTACC 
     2461 AGTGCAACTT CTGTTCCGAA GTTGTCAATG ACCTCAACAC CCTTCAGGAG CACATCCGAT 
     2521 GTTCTCATGG GTTTGCCAAT CCCGCGGCCA AGGACAGCAA TGCGTTCTTT TGTCCCCATT 
     2581 GTTACATGGG GTTTCTCACT GACTCTTCAC TTGAAGAGCA TATAAGACAG GTCCATTGTG 
     2641 ACCTCAGTGG CTCCCGGTTT GGGTCTCCTG TGCTTGGGAC TCCAAAAGAA CCGGTGGTTG 
     2701 AAGTCTACTC CTGTTCCTAT TGTACAAATT CGCCAATATT CAACAGTGTT CTTAAACTGA 
     2761 ATAAGCATAT TAAAGAGAAT CATAAAAACA TTCCCTTGGC CCTGAATTAT ATTCACAATG 
     2821 GGAAGAAATC CCGGGCCTTG AGCCCTTTGT CCCCTGTGGC TATAGAACAA ACAACTCTTA 
     2881 AGATGATGCA GACTGTGGGA GGCGGGCCTG CCCGCGCCTC TGGAGAGTAT ATCTGTAATC 
     2941 AGTGTGGTGC TAAGTACACG TCCCTAGACA GCTTTCAGAC TCACCTCAAA ACCCATTTGG 
     3001 ACACCGTGCT GCCAAAACTG ACCTGCCCTC AGTGTAACAA AGAATTCCCC AACCAAGAGT 
     3061 CCTTGCTAAA GCACGTGACC ATCCACTTTA TGATCACCTC AACCTACTAC ATCTGTGAGA 
     3121 GCTGTGACAA GCAGTTCACC TCCGTGGATG ACCTCCAGAA GCACCTGCTG GACATGCACA 
     3181 CCTTCGTCTT CTTCCGCTGC ACACTCTGCC AGGAAGTGTT CGACTCCAAG GTCTCTATCC 
     3241 AGCTGCACTT GGCCGTGAAG CACAGTAACG AGAAGAAGGT GTACCGCTGC ACGTCCTGCA 
     3301 ACTGGGACTT CCGCAACGAG ACCGACCTGC AGCTGCACGT GAAGCACAAC CACTTGGAGA 
     3361 ACCAAGGCAA AGTGCACAAG TGCATCTTCT GCGGCGAGTC GTTTGGCACC GAGGTGGAGC 
     3421 TGCAGTGCCA CATCACCACC CACAGCAAGA AGTACAACTG CAGGTTCTGC AGCAAAGCCT 
     3481 TCCACGCCGT CATCCTGCTG GAGAAGCACC TGCGGGAGAA ACACTGTGTG TTTGAAACCA 
     3541 AGACCCCCAA CTGTGGCACC AACGGGGCCT CGGAGCAAGT GCAGAAGGAG GAGGCTGAGC 
     3601 TGCAGACCTT GCTCACCAAC AGCCAGGAGT CCCATAACAG CCATGATGGG AGTGAGGAGG 
     3661 ACGTGGACAG CTCTGAACCC ATGTATGGCT GTGACATCTG CGGGGCGGCC TACACCATGG 
     3721 AGACGTTGCT GCAGAACCAC CAGCTCCGAG ACCACAACAT CAGGCCCGGA GAAAGCGCCA 
     3781 TCGTGAAGAA GAAGGCGGAG CTCATTAAAG GGAATTACAA GTGTAACGTT TGCTCGAGAA 
     3841 CCTTCTTCTC CGAAAACGGG CTCCGTGAAC ACATGCAGAC CCACTTAGGC CCGGTCAAAC 
     3901 ACTACATGTG CCCTATCTGT GGAGAGCGCT TCCCTTCCCT GTTAACTCTC ACAGAGCACA 
     3961 AAGTCACACA CAGCAAGAGC CTGGATACCG GAAACTGTCG TATTTGTAAG ATGCCCCTGC 
     4021 AGAGCGAAGA GGAGTTTTTG GAGCACTGCC AGATGCACCC TGACCTGAGG AATTCCCTGA 
     4081 CTGGGTTTCG TTGTGTGGTG TGTATGCAGA CTGTGACCTC CACCTTGGAA CTCAAAATCC 
     4141 ACGGGACCTT CCACATGCAG AAGACGGGGA ATGGGTCCTC CGTGCAGACC ACCGGGCGTG 
     4201 GCCAGCACGT TCAGAAACTG TATAAGTGCG CATCTTGCCT CAAAGAGTTC CGTTCCAAGC 
     4261 AAGATCTGGT GAAACTCGAC ATCAACGGCC TGCCATATGG TCTGTGTGCC GGCTGTGTGA 
     4321 ATCTCAGTAA GAGCAGTAGC CCGGGCCTCA GTCTCCCTCC TGGTGCCAGC AGGCCAGGCT 
     4381 TGGGCCAGAA TGAGAGTCTG AGCGCCATGG AAGGAAAAGG CAAGGCGGGG GGACTGAAGA 
     4441 CGCGCTGTTC AAGCTGCAAT GTCAAGTTTG AGTCTGAAAG CGAACTGCAG AACCACATCC 
     4501 AGACGGTGCA CCGGGAGCTT GTGCCAGACG CCAACAGCAC ACAGTTGAAA ACGCCCCAAG 
     4561 TGTCACCCAT GCCCAGAATC AGTCCCTCCC AGTCCGATGA GAAGAAGACC TACCAGTGTA 
     4621 TCAAGTGTCA GATGGTTTTC TACAACGAGT GGGACATCCA GGTTCATGTG GCAAATCACA 
     4681 TGATTGATGA AGGGCTGAAC CATGAGTGCA AACTCTGCAG CCAGACCTTT GACTCCCCCG 
     4741 CCAAACTTCA GTGTCACCTG ATAGAGCACA GCTTCGAAGG GATGGGTGGT ACCTTCAAGT 
     4801 GCCCCGTCTG TTTCACAGTG TTTGTTCAAG CTAACAAGTT GCAACAGCAT ATATTCTCTG 
     4861 CCCATGGACA AGAAGACAAG ATCTATGACT GCACACAATG CCCACAGAAG TTTTTCTTCC 
     4921 AAACAGAGCT ACAGAATCAT ACGATGACTC AGCACAGCAG TACGCGTACG CGGCCGCTCG 
     4981 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5041 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5101 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5161 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5221 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5281 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5341 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5401 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5461 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5521 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5581 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5641 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5701 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5761 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5821 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5881 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5941 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6001 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6061 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6121 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6181 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6241 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6301 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6361 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6421 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6481 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6541 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6601 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6661 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6721 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6781 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6841 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6901 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6961 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7021 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7081 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7141 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7201 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7261 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7321 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7381 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7441 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7501 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7561 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7621 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7681 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7741 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7801 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7861 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7921 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7981 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8041 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8221 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8281 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8341 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8401 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8461 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8521 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8581 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8641 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8701 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8761 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//