LOCUS       MR211898                8769 bp    DNA     circular  SYN 
DEFINITION  Nfrkb (Myc-DDK-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb)
ACCESSION   NM_172766
VERSION     NM_172766.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4926..5017 
                     /label="Myc-DDK"  
     misc_feature    5047..5632 
                     /label="PolyA signal"  
     misc_feature    5104..5123 
                     /label="Restriction Enzyme"  
     misc_feature    5908..6580 
                     /label="ColE1"  
     misc_feature    7010..7804 
                     /label="Kan/Neo"  
     misc_feature    7839..8141 
                     /label="SV40 ori"  
     misc_feature    8227..8704 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4908..4914 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4908 
                     /label="Nfrkb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTCCTTG GACCACATGC TGACAGATCC CCTTGAACTT GGTCCTTGTG 
     1081 GAGATGGTCA CAGTACAGGA ATCATGGAGG ACTGCCTTCT GGGTGGTACC AGGGTTAGTC 
     1141 TGCCCGAAGA CCTTCTGGAG GATCCCGAGA TATTCTTTGA TGTCGTCAGC CTGTCCACAT 
     1201 GGCAGGAAGT ATTAAGTGAC TCTCAGCGTG AGCACCTCCA GCAGTTCCTG CCTCGGTTTC 
     1261 CTGCGGACAG TGTGGAGCAG CAGAGGGAGC TCATCCTTGC CCTATTCAGT GGGGAGAACT 
     1321 TCCGCTTCGG AAACCCGCTG CACATCGCCC AGAAGCTCTT CCGAGATGGA CACTTTAACC 
     1381 CTGAGGTGGT CAAGTATCGT CAGCTCTGCT TCAAGTCACA GTACAAGCGG TATCTCAACT 
     1441 CCCAACAACA GTATTTCCAC CGGCTGCTGA AACAGATTCT TGCTTCCAGG AGTGATCTCT 
     1501 TGGAGATGGC TCGTAGGAGT GGCCCTGCTC TTCCCTTTCC ACACAAGCAC CATTCACCGT 
     1561 CCCGAAGCCC CGAGGAGCGG GAGTGGCGGA CCCAGCAGCG TTACTTGAAA GTCTTGAGGG 
     1621 AAGTGAAGGA GGAATGTGGG GACACTGCTC TGTCCTCTGA TGAGGAGGAT CTCAGCTCAT 
     1681 GGCTTCCAAG CTCTCCAGCA CGTTCTCCTA GTCCTGCGGT GCCCCTGAGG GTGGTGCCCA 
     1741 CGCTTTCCAC CACGGATATG AAAACTGCAG ATAAAATAGA ACTGGGGGAC AGTGACCTGA 
     1801 AGTTAATGTT AAAGAAGCAC CATGAGAAGC GGAAACATCA ACCAGATCAC CCAGACCTTT 
     1861 TGACAGGGGA CCTGACCCTC AGTGACATCA TGACTCGAGT AAATGCCGGC AGGAAGGGCT 
     1921 CCCTAGCAGC CTTGTATGAT TTGGCTGTTC TTAAAAAAAA GGTGAAGGAA AAGGAGGAGA 
     1981 AGAAAAAGAA GAAAATAAAG TTGATTAAGT CGGAAGCAGA GGATCTGGCT GAGCCCCTAA 
     2041 GCAGTACTGA AGGGGTCCCA ACTCTCTCAC AGGCCCCCTC TCCCCTGGCA ATATCGTCTA 
     2101 TCAAGGAGGA ACCCCTGGAA GACATCAAGC CTTGCCTTGG AATCAATGAA ATATCTTCCA 
     2161 GTTTTTTCTC TCTTCTGTTA GAGATCTTGC TGCTGGAAAG TCAGGCTAGC CTTCCTATGC 
     2221 TGGAGGATCG AGTTTTGGAC TGGCAGTCTT CTCCAGCCAG CTCCCTCAAC AGCTGGTTCT 
     2281 CTGCTGCCCC CAACTGGGCT GAGTTGGTGT TGCCTGCTCT GCAGTATCTT GCTGGAGAAA 
     2341 GCCGAGCGGT TCCTTCTAGT TTCTCTCCAT TTGTTGAATT CAAAGAGAAA ACCCAGCAGT 
     2401 GGAAATTGCT TGGTCAATCT CAAGATAATG AAAAGGAATT AGCTGCTCTC TTCCACCTGT 
     2461 GGCTGGAAAC CAAAGACCAG GCCTTCTGTA AGGAAAATGA AGACAGCTCA GATGCCATGA 
     2521 CACCTGTCCC TCGAGTAAGA ACTGACTATG TGGTTCGGCC TAGCACAGGA GAGGAGAAAC 
     2581 GGGTTTTTCA AGAACAGGAG CGTTACAGGT ATAGCCAACC CCATAAGGCA TTCACCTTTC 
     2641 GCATGCATGG CTTTGAGTCT GTGGTGGGGC CAGTGAAGGG CGTGTTTGAC AAGGAGACCT 
     2701 CCCTCAACAA GGCTCGTGAG CATTCACTGC TGCGCTCTGA CCGGCCTGCC TATGTCACCA 
     2761 TTCTGTCTCT TGTTCGGGAT GCTGCAGCTC GGCTGCCTAA TGGAGAAGGC ACTCGGGCAG 
     2821 AGATCTGTGA ACTGCTCAAG GACTCTCAGT TTCTGGCTCC AGATGTCACC AGCACTCAGG 
     2881 TGAACACAGT GGTGAGTGGC GCACTGGATC GATTGCATTA TGAGAAAGAC CCTTGTGTGA 
     2941 AATATGACAT CGGTCGGAAG CTGTGGATCT ACCTGCATCG TGACCGGAGT GAGGAAGAGT 
     3001 TTGAACGGAT TCATCAAGCT CAAGCAGCGG CAGCTAAAGC CAGAAAAGCT CTTCAACAAA 
     3061 AACCCAAGCC GCCATCCAAG GTGAAGTCCA GTAATAAGGA GGGCTCCACG AAGGGCCTTA 
     3121 GCGGTCCTTC CGAGCAGAGC CAGATGAGCC TCAGTGACTC TAGCATGCCG CCCACCCCAG 
     3181 TTACGCCTGT AACACCCACC ACGCCAGCAT TGCCCACCCC CATCTCTCCT CCACCCGTGT 
     3241 CAGCGGTGAA CAGAAGTGGC TCTAGCACGG TCTCTGAGCC AGCCCAGTCT AGTTCAGGTG 
     3301 TGCTTCTGGT GTCCTCACCG ACAATGCCAC AGCTAGGGAC GATGCTTTCC CCAGCTTCCA 
     3361 TCCAGACTCC ACCCAGTTCT CAGGCCACTG CTCGGGTTGT CAGCCATTCC AGCTCAGCAG 
     3421 GACTACCCCA GGTTCGGGTG GTAGCCCAGC CCAGCCTTCC TGCTGTTTCC CAGCAGTCAG 
     3481 TAGGGCCAGC ACAGCCACTA CCCCAGATGC CAGCAGGACC ACAGATTCGT GTGCCAGTCA 
     3541 CTGCTACTCA AACCAAAGTA GTACCCCAGG CAGTTATGGC AACAGTTCCA GTCAAGGGGC 
     3601 AAACTGCAGC AGCCTCTGTG CAACGGCCTG GACCTGGGCA GACAGGGCTC ACAGTGACGA 
     3661 ATCTTCCGGC TGCAGTCAGC CCCGTGAGCA AGACAGCCAT GAGTTCTCCT GGGAACTCTG 
     3721 CTCCAAGTGC CTCCACCACA GCCGTTATTC AGAACGTCAC AGGACAGAAC ATCATCAAGC 
     3781 AGGTGTCGAT CACTGGACAG CTTGGTGTGA AGCCCCAGAC AGGCAGCAGC ATTCCACTCA 
     3841 CAGCCACAAA CTTCCGTATC CAGGGTAAGG ATGTACTGCG CCTGCCGCCT TCTTCCATCA 
     3901 CCACAGATGC CAAGGGCCAG ACGGTTTTGA GAATCACTCC AGACATGATG GCCACATTGG 
     3961 CCAAGTCTCA GGTTACCACA GTCAAACTGA CTCAGGACCT CTTTGGAGCA GGAAGTGGCA 
     4021 CTGCAGGCAA AGGCATCTCT GCTACCTTGC ATGTTACTTC CAACCCTGTC CACGCGGCTG 
     4081 ACAGCCCTGC CAAGGCCCCT TCAGCCAGTG TCCCTTCATC AGCTCCAGCA GGTACTACTG 
     4141 TGGTCAAAGT AACTCCTGAT CTCAAGCCGA CAGAAACTGC AAATTCAGCT TTTCGCTTGA 
     4201 TGCCAGCTCT TGGTGTAAGT GTGGCAGATC AGAAGGGAAA GAACACAGTG GCCTCTTCAG 
     4261 AAGCAAAACC TGCCGCCACA ATCCGCATCG TGCAGGGGCT GGGAGTGATG CCCCCTAAAG 
     4321 CAGGCCAGAC CATTACTGTT GCAGCACATG CAAAGCAAGG AGCCTCTGTT GCTGGAGGGT 
     4381 CTGGAACTGT CCATTCTTCA ACGGTGTCCT TACCCAGTAT AAATGCCGCT GTGTCAAAGA 
     4441 CTGTGGCTGT GGCCTCTGGG GCAACAAGCA CCCCCATCAG CATCGGGACT GGAGCCCCCA 
     4501 CCGTGCGACA GGTTCCTGTT AACACTACAG TTGTGTCCAC ATCCCAGTCT GGGAAACTGC 
     4561 CTACCAGAAT CACAGTTCCT CTTTCTGTGA TTAGCCAGCC AATGAAGGGC AAGAGCGTGG 
     4621 TCACAGCCCC CATCATCAAA GGCAACCTTG GAGCCAATCT CAGTGGGCTG GGTCGCAACA 
     4681 TCATCCTCAC GACCATGCCA GCAGGTACCA AACTCATTGC TGGCAATAAG CCAGTGAGTT 
     4741 TCCTCACTGC TCAGCAGTTG CAGCAGCTTC AGCAACAAGG TCAGGCTACA CAGGTGCGCA 
     4801 TCCAGACCGT CCCGGCATCC CATCTGCAAC AGGGCACGGC TTCTGGCTCC TCCAAAGCAG 
     4861 TGTCCACTGT TGTTGTGACC ACAGCTCCAT CTCCTAAACA AGCACCTGAG CAGCAGACGC 
     4921 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4981 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5041 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5101 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5161 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5221 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5281 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5341 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5401 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5461 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5521 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5581 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5641 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5701 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5761 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5821 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5881 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5941 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6001 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6061 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6121 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6181 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6241 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6301 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6361 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6421 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6481 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6541 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6601 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6661 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6721 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6781 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6841 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6901 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6961 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7021 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7081 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7141 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7201 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7261 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7321 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7381 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7441 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7501 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7561 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7621 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7681 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7741 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7801 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7861 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7921 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7981 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8221 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8281 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8341 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8401 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8461 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8521 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8581 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8641 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8701 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8761 GCGAATTTT
//