LOCUS       MR211894                8754 bp    DNA     circular  SYN 
DEFINITION  Oplah (Myc-DDK-tagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah)
ACCESSION   NM_153122
VERSION     NM_153122.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4909..5000 
                     /label="Myc-DDK"  
     misc_feature    5030..5615 
                     /label="PolyA signal"  
     misc_feature    5087..5106 
                     /label="Restriction Enzyme"  
     misc_feature    5891..6563 
                     /label="ColE1"  
     misc_feature    6993..7787 
                     /label="Kan/Neo"  
     misc_feature    7822..8124 
                     /label="SV40 ori"  
     misc_feature    8210..8687 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4891 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="Oplah"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCCCA GAAGAGCGCT TCCATTTTGC CATCGACCGT GGTGGCACCT 
     1081 TTACAGATGT CTTTGCCCAG TGCCCAGGAG GGCATGTACG TGTCCTGAAG CTGCTCTCAG 
     1141 AGGATCCTGC CAACTATGCA GACGCACCCA CAGAGGGCAT CCGCCGAATT CTAGAGCAGG 
     1201 AGAGGGGTGT GCTGCTGCCC CGAGGCCGGC CGCTAGACAC CAGTCACATC GCCAGCATCC 
     1261 GCATGGGTAC CACCGTGGCC ACCAATGCTC TGTTGGAACG GCAGGGAGAA CGGGTGGCAC 
     1321 TGCTGGTGAC TCGCGGTTTC CGAGACCTGC TGCATATTGG CACTCAGGCC CGCCCAGACC 
     1381 TCTTTGACCT GGCTGTGCCC ATGCCAGAGG TACTGTATGA GGAGGTCGTG GAGGTGGACG 
     1441 AGCGAGTGCT GCTGTATCGT GGAGAACCCG GTGCCGGCTC TCCCGTCAAA GGCTGTACAG 
     1501 GGGACCTGCT AGAGATACAG CAGCCTGTGG ATCTGGCAGC CCTGCGTGGG AAGCTGGAGG 
     1561 GGCTCTTGAC TCGGGGCATT CACAGCCTGG CAGTGGTACT CATGCATTCG TACACGTGGG 
     1621 CCCAGCACGA GCAGCAGGTG GGCACACTGG CCCGGGAGCT GGGCTTCACG CACGTCTCCT 
     1681 TGTCCTCGGA AGTCATGCCC ATGGTGCGGA TTGTTCCTCG GGGGCATACG GCCTGTGCAG 
     1741 ATGCTTACCT CACTCCCACC ATCCAGCGCT ATGTGCAGGG CTTCCGCCGA GGCTTCCAGG 
     1801 GCCAGCTAAA GAATGTGCAA GTCCTCTTCA TGCGCTCCGA TGGTGGCCTG GCACCCATGG 
     1861 ATGCCTTCAG TGGCTCCCGG GCTGTGCTCT CTGGCCCTGC TGGTGGTGTG GTTGGCTACT 
     1921 CCACTACCAC CTACCAGCTG GAAGGTGGTC AGCCTGTCAT TGGCTTTGAC ATGGGAGGCA 
     1981 CATCCACAGA TGTGAGCCGC TATGCTGGAG AGTTTGAGCA CGTCTTTGAG GCTAGCACGG 
     2041 CAGGTGTTAC CCTCCAGGCC CCACAGCTGG ACATCAATAC GGTGGCAGCT GGCGGGGGTT 
     2101 CCCGCCTCTT CTTCAGGTCT GGCCTCTTTG TGGTTGGTCC AGAGTCAGCA GGTGCCCACC 
     2161 CAGGTCCTGC CTGCTACCGC AAAGGGGGTC CTGTGACAGT GACAGATGCT AATCTGGTTC 
     2221 TGGGTCGCCT GCTGCCTGCC TCCTTCCCCT GCATTTTTGG GCCAGGAGAA GACCAGCCAT 
     2281 TGTCTCCCGA GGCTTCCCGA AAGGCTCTAG AGGCTGTGGC CATGGAGGTC AACAGTTTCT 
     2341 TGGCCAGTGG ACCCTGCCCA GCTTCCCAGC TAAGTCTGGA GGAGGTGGCC ATGGGGTTTG 
     2401 TGCGTGTTGC CAACGAAGCC ATGTGCCGGC CTATCCGTGC CCTCACACAG GCACGAGGCC 
     2461 ACGACCCCTC AGCCCATGTA CTGGCTTGCT TTGGAGGAGC CGGTGGGCAA CACGCTTGTG 
     2521 CCATTGCCCG GGCCCTGGGT ATGGACACTG TGCATATTCA CAGGCACAGC GGGCTGCTGT 
     2581 CAGCACTGGG ACTGGCCTTG GCCGATGTGG TTCACGAAGC ACAGGAGCCC TGTTCCCTGT 
     2641 CTTACACACC TGAAACCTTT GCACAACTGG ACCAGAGACT GAGCCGCCTG GAGGAGCAGT 
     2701 GTGTGGATGC CCTGCAGGCC CAGGGCTTCT CTAGGTCTCA GATCAGCACC GAGAGCTTCC 
     2761 TGCACCTTCG CTACCAAGGC ACTGACTGTG CCCTAATGGT GTCTGCCAAT CAGCATCCGG 
     2821 CCACAACCTG CTCACCCCGT GCTGGTGACT TTGGAGCAGC GTTTGTCGAG AGGTACATGA 
     2881 GAGAGTTTGG CTTCATCATT CCTGAGCGCT CGGTGGTGGT AGATGATGTG CGTGTGAGGG 
     2941 GAACTGGCCG TAGTGGACTT CAGCTGGAGG AGACCTCCAA AATCCAGAGT GGACCTCCGC 
     3001 ATGTGGAAAA GGTGACCCAG TGCTACTTTG AAGGGGGTTA TCAGGAGACT CCCGTGTACC 
     3061 TTTTAGGAGA ACTAGGCTAC GGGCATCAGC TTCAAGGGCC CTGCCTGATC ATTGACAACA 
     3121 ACAGCACCAT CCTTGTAGAA CCAGGTTGCC AAGCGGAGGT GATTGAGACA GGGGACATCC 
     3181 GCATTTCTGT GGGAGCCGAG GCTCCCAGTA TGATAGACAC CAAGCTTGAC CCCATCCAGT 
     3241 TGTCTATTTT CTCACACCGT TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTACAGC 
     3301 GCACAGCCAT CTCTACCAAC ATCAAGGAAC GCCTCGACTT CTCCTGTGCC CTCTTTGGGC 
     3361 CAGATGGGGG CCTCGTCTCC AATGCTCCCC ACATTCCTGT GCACCTGGGT GCCATGCAAG 
     3421 AGACTGTACA GTTCCAGATT CAGCACTTAG GAGCCGACCT CCATCCTGGT GATGTGTTGC 
     3481 TTAGTAACCA TCCCAGTGCA GGGGGCAGCC ATCTTCCTGA CCTGACTGTC ATCACACCGG 
     3541 TGTTTTGGCC AGGTCAGTCG AGGCCTGTGT TCTACGTGGC TAGCCGAGGG CACCACGCGG 
     3601 ACATTGGAGG TATCACCCCG GGTTCTATGC CTCCTCACTC TACCACACTG CAGCAGGAGG 
     3661 GTGCCGTTTT TCTGTCCTTC AAACTGGTCC AGGGAGGAGT CTTCCAGGAA GAGGCGGTGA 
     3721 CAGAGGCCTT ACAGGCCCCA GGCAAGATCT CTGGCTGCAG TGGGACCAGG AACCTGCATG 
     3781 ACAACCTGTC GGATCTCCGT GCCCAGGTGG CAGCTAACCA GAAAGGCATC CAGCTGGTGG 
     3841 GAGAGCTGAT CGGACAGTAT GGCTTAGATG TGGTGCAGGC CTATATGGGC CATATTCAGG 
     3901 CAAATGCTGA ACTAGCAGTG CGAGACATGC TTCGTGCTTT TGGAACTTCC CGCCAGGCCA 
     3961 GGGGCCTGCC CCTGGAGGTG TCTGCCAAGG ATCACATGGA CGATGGCTCT CCCATCTGCC 
     4021 TGCATGTTCA GATCAACCTG AATCAGGGCA GCGCTGTATT TGACTTCAGT GGTTCTGGGT 
     4081 CTGAGGTGTT TGGCAATCTC AATGCCCCTC GAGCCATAAC ACTGTCTGCT CTCATCTATT 
     4141 GCTTGCGCTG TCTAGTGGGC CGTGACATCC CACTTAACCA GGGTTGCCTG GCTCCTGTGC 
     4201 AAGTCATAAT TCCCAAAGGC TCCATATTGG ATCCATCCCC AGAGGCAGCA GTGGTCGGAG 
     4261 GCAACGTGCT CACATCTCAG CGAGTCGTGG ATGTCATCCT GGGGGCTTTT GGGGCCTGTG 
     4321 CAGCCTCCCA GGGCTGCATG AACAATGTGA CCCTGGGCAA TGCCCGTATG GGCTACTATG 
     4381 AGACAGTAGC TGGTGGTGCG GGTGCAGGCC CTGGCTGGCA TGGGCGCAGT GGTGTACACA 
     4441 GTCACATGAC CAACACGCGC ATTACGGACC CAGAGATTTT GGAGAGCCGG TATCCAGTTA 
     4501 TCCTGCGCCG CTTTGAGCTG AGGCCAGGCT CTGGAGGCCG AGGCCGCTTC CGGGGAGGTG 
     4561 ATGGCGTAGT CCGAGAGCTG GTCTTTCGGG AAGAGGCGCT ACTGTCTGTC CTCACGGAGC 
     4621 GCCGCGCCTT CCAGCCTTAT GGCCTCCACG GGGGAGAGCC TGGTACACGT GGCTTAAACC 
     4681 TCCTGATCAG AAAAGATGGG CGCACAGTGA ATCTGGGCGG CAAGACATCC GTGACTGTGT 
     4741 ATCCCGGGGA CGCGTTCTGC CTCCACACGC CTGGGGGTGG GGGCTATGGA GACCCGGAGG 
     4801 ACCCAGCACC ACCACCAGGC TCGCCCCCGC TATATCCAGC CTTCCCAGAG CGAGGCAGTG 
     4861 TGTATGAGTA CCGCCGCGCC CAGGAAGCAG TAAGCGGACC GACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//