LOCUS       MR211886                8715 bp    DNA     circular  SYN 
DEFINITION  Smchd1 (Myc-DDK-tagged) - Mouse SMC hinge domain containing 1 (Smchd1)
ACCESSION   NM_028887
VERSION     NM_028887.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4872..4963 
                     /label="Myc-DDK"  
     misc_feature    4993..5578 
                     /label="PolyA signal"  
     misc_feature    5050..5069 
                     /label="Restriction Enzyme"  
     misc_feature    5854..6526 
                     /label="ColE1"  
     misc_feature    6956..7750 
                     /label="Kan/Neo"  
     misc_feature    7785..8087 
                     /label="SV40 ori"  
     misc_feature    8173..8650 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4854..4860 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4854 
                     /label="Smchd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAACTC CCAGGGACAA GTCATGGAGG GTCAAAGAAG CTGCTGGTGG 
     1081 AACTTAAAGT CATACTGCAC ACTTCAAGTG GAAATAAAGA AATCATTTCA CATATCAGTC 
     1141 AGCATGGAGG GAAATGGCCT TACTGGTTTA AAAAAATGGA AAATATCCAG AAACTGGGTA 
     1201 ATTATACATT GAAATTACAA GTTGTATTGA ATGAAAGTAA TGCAGATACC TATGCAGGAA 
     1261 GATCACTACC GTCCAAAGTA ATTAAATTCT CTGTTAAAGA GGGCAAGCCA GAAAAGTTTT 
     1321 CGTTTGGTCT TCTGGACTCT CCTTTCCGTG TTGGAGTTCC ATTTAATATT CCTCTGGAGT 
     1381 TACAGGATGA ATTTGGTCAC ACTACCCAAC TATTGTCTGA TATTGAGCCA GTTCTTGAAG 
     1441 CAAGTGGTCT GTCTTTACAT TATGAAGGAA TAACCAAAGG CCCGAATTGT GTAATTCAAG 
     1501 GTGTCGTAGC TAAAGGTCCA GTTAACTCTT GCCAGGGCAA GAATTTTAAT CTGAAGGTTA 
     1561 TTCTTCCTGG TTTAAAAGAA GACTCACAGA TTTTGAAGAT TAGATTGCTA CCTGGTCCCC 
     1621 CTCATCAACT AAAGGTGAAA CCTGACTCAG AAGTTCTGGT TATAGAGAAT GGAACAGCTT 
     1681 TCCCATTTCA GGTGGAAGTT GTGGATGAGT CAGACAACAT AACAGCACAA CCAAAGCTGA 
     1741 TCGTCCACTG TAAGTTTCTA GGTGCTCCAA ACCTACCAGT CTATACTGTG GATTGCAGTA 
     1801 GCTCTGGAAC TAGCATCCTA ACAGGGTCAC CCATTCAAGT TCAGAACATT AAGAAAGACC 
     1861 AGAAGACACT GACCGCCAGG ATTGAGATAC CTAGCTGTAA AGATGTGTCA CCTGTTGAAA 
     1921 AGACGATCAA GTTACTTCCC AGTAGCCACG CTGCTTGCCT GCAGATCTTT AGTGTAGAGG 
     1981 AGCAAAAGGC GATCCAGATC AAACATCAGG ATGAGGTTAC TTGGGTAGCA GGTGATGTAA 
     2041 TACGCAATCT AATTTTTCAA ATGTATGACG AAGGAGAAAG AGAAATCAAT ATTACACCAT 
     2101 CCTTGGCAGA AAAAATTAAG GTTAATTGGA CTCCTGAGGT GAACAAAGAA CATTTGGTAC 
     2161 AAGGTCTCCT TCCTGATGTG CAAGTACCAA CATCTGTGAA AGATGTCCGC TACTGCCATG 
     2221 TTTCTTTCCA AGATGACCAC GTGTGTCTGG AGAGCGCCTT TACAGTAAGA CCACTTCCTG 
     2281 ATGATCCCAA ACATTTAAAA TGTGAATTAA AAGGAGGAAA AACAGTGCAG ATGGGCCAAG 
     2341 AGCTTCAAGG AGAAATAGTT GTAATAATTG CAGATCAGTA TGGAAATCAG ATTTCATCAT 
     2401 TTTCACCTGA TTCCTTATCT ACTTTGTCGA TTACTGGAGA TGGCCTTGAC AGCTCAAACT 
     2461 TGAAAATCAC CTTGGAGGCC AACTCACAGA GCGTAAGTGT GCAAGGCATC AGGTTTACTC 
     2521 CAGGGCCTCC TGGACCCAAG GATCTGTGTT TTACTTGGCG AGAGTTTTCT GACTTTCTGC 
     2581 GCGTGCAACT GGTTTCTGGA CCTCCAACCA AGCTGCTGCT TATGGACTGG CCAGAGCTGA 
     2641 AAGAGTCCAT TCCTGTGATT AATGGAAGAC AATTAGAGAA CCCTCTCATT GTTCAACTTT 
     2701 GTGATCAGTG GGATAATCCT GCTTTAGTCC CAAACGTTAA AATATGTCTC ATAAAAGCAA 
     2761 GCAGCTTAAG GCTACTACCT TCAAACCAGC AGCATAAAAC AGATGATAAG GGCAGAGCTA 
     2821 ATTTGGGGGT GTTCACTGTT TGTGCTCCAA GGGGAGAACA CACTGTGCAG GTTAAAGGTG 
     2881 TCTATAATAA GAGCACTATA GAAGGGCCTA CAATTAAGTT AACAATTCTT CCAGACCCTG 
     2941 AAAAGCCCAT TCGCCTCAAT GTTAAATATG ACCAAGATGC CTCTTTTATA GCAGGGGACA 
     3001 TATTCACTGA TTTTATGGTT AGTGTGATTT CTGAAAGTGG TAGTGTCATC AAAAATATTA 
     3061 ATCCAACACG TATTTCCATG AAAATGTGGA AGCTGTCCAG TGGCATGAGC CGGCCACCAG 
     3121 CAAATGCAGA AACCTTTAGT TGTAATAAAA TAAAAGGTAA CGACAAGGAA GATGGCTGCT 
     3181 TCTATTTCAG GGAGAAAACT ATCCCTAACA AGGTGGGAGC ATATTGTATC CAGTTTGATT 
     3241 TCATGATTGA TAAGACAAAT ATTCTAAGCA GCCAACAGGT TATAGTTGAT GTTCTCCCCA 
     3301 ACCAGCCTAT GAAGTTGGTT CCTGACAGCC AACCAGCTAC CCCAGCTGTT TCTAATGTCC 
     3361 GTTCAATTGC CAGCAGGACC TTGGTCAAAG ACCTGCGCCT CAGTATCACA GATAATTATG 
     3421 GTAATCACAC TGGAATGGAT CTGGTTGGCA CTGTAGTAGC AACCATTAAA GGCTTTAATG 
     3481 AGGAGGACAC TGATACCCCA CTCTTCATCG GGAAGGTTAG GACGCTTGAG TTTCCCTTTG 
     3541 TGAAAGGCTC TGCTGAAATC ACGACTCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA 
     3601 GTACTGAATA CTTTATTATC TTTGAGCCTC GGCTGTCAAC TGTATCAGGA ACATTAGAAT 
     3661 CATATAGCCT GCCATTTATG TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCGC 
     3721 TTACGAAGGA GAAGGACGAG TTATCCAAGT CCATCACTAT GTACAGAAGT CTCTTCGATG 
     3781 CCAACAAGCA GCTTGTGGAT GAAATGAAAT GCCAAGCTGA AGAAGCAAAG TTAAAAGAGA 
     3841 CCCAGCTACG AAATGAACTA AAAGCGTACA ATATTGATAT TCCTGCGACA CAACAGACAA 
     3901 CTCATATTGA AGCACTTTTG GAAAAAAAGA TAACAGAACA GAACGAGCTT AAAAAGAGGC 
     3961 CCAGAAGGTT GTGTACTCTT CCAAACTACA CTAAACGCAG TGGAGATATT TTGGGAAAGA 
     4021 TTGCACATCT AGCACAAATT GAAGATGATC GAGCTGCCAT GGTTATCTCT TGGCATCTGG 
     4081 CAAGTGACAT GGACTGTGTG GTTACCCTAA CCACTGATGC CGCACGCGCT ATCTATGACG 
     4141 AAACTCAAGG TCGCCAGCAA GTGTTGCCCC TTGATTCTAT TTACAGGAAA ACTCTTCCAG 
     4201 ATTGGAAAAG ACCTCTACCT CATTTCCGAA ATGGTAAATT ACACTTTAAG CCCTTTGGAA 
     4261 ATCCAGTCTT TGCCCGAGAT TTGTTAACAT TCCCAGATAA TATTGAACAT TGTGAAACAG 
     4321 TTTTTGGTAT GCTGTTAGGA GACACCATTA TTTTGGATAA TCTGGATGCA GCCAATCATT 
     4381 ATAGAAAAGA GGTTGTTAAA ATCACACACT GCCCCACACT CCTGACTAGA GATGGAGATA 
     4441 GAATTAGAAG TAATGGGAAG TTTGGGGGCC TTCAGAATAA AGCTCCGCCA ATGGACAAAC 
     4501 TTCGGGGAAT GGTCTTCGGA GCTCCAGTAC CAAAGCAGTG TGTGGTCTTG GGGAAACAAA 
     4561 TAGATCTTAT TCAGCAGTAT CGCACTGCTC TGTACAGGCT GAGCTCTGTG AACGAGGACT 
     4621 TGGACAATCA GCTGCAGTAC CTTCACACTC CTGATATGAA GAAGAAAAAG CAAGAACTTG 
     4681 ATGAACAGGA AAAAAGTCTG AAACGAATAG AGCAGAAACT CGGAATGACT CCTGTGCGCA 
     4741 GGTGTAATGA GTCACTTTGT CACTCACCAA AGATTGAGGT GACAGAGTGC CCAATCCCTA 
     4801 CTAAAAGAAT GAGAAGAGAA TCCACCAGAC AGAATAGAAG GCCAAAAGGT GACGTACCAA 
     4861 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4921 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4981 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5041 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5101 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5161 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5221 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5281 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5341 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5401 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5461 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5521 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5581 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5641 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5701 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5761 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5821 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5881 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5941 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6001 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6061 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6121 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6181 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6241 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6301 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6361 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6421 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6481 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6541 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6601 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6661 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6721 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6781 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6841 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6901 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6961 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7021 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7081 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7141 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7201 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7261 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7321 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7381 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7441 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7501 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7561 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7621 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7681 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7741 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7801 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7861 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7921 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7981 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8161 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8221 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8281 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8341 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8401 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8461 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8521 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8581 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8641 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8701 TTTAACGCGA ATTTT
//