LOCUS       MR211885                8712 bp    DNA     circular  SYN 
DEFINITION  Npc1 (Myc-DDK-tagged) - Mouse Niemann Pick type C1 (Npc1)
ACCESSION   NM_008720
VERSION     NM_008720.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4869..4960 
                     /label="Myc-DDK"  
     misc_feature    4990..5575 
                     /label="PolyA signal"  
     misc_feature    5047..5066 
                     /label="Restriction Enzyme"  
     misc_feature    5851..6523 
                     /label="ColE1"  
     misc_feature    6953..7747 
                     /label="Kan/Neo"  
     misc_feature    7782..8084 
                     /label="SV40 ori"  
     misc_feature    8170..8647 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4851..4857 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4851 
                     /label="Npc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGCGCAC CACCCGGCCC TCGGCCTGCT GCTGCTGCTG CTGTGCCCTG 
     1081 CGCAGGTGTT TTCGCAATCC TGTGTTTGGT ATGGAGAGTG TGGAATTGCG ACTGGAGATA 
     1141 AGAGGTACAA CTGTAAATAT TCTGGCCCAC CAAAACCCCT CCCAAAGGAC GGCTATGACT 
     1201 TAGTGCAGGA ACTCTGTCCA GGACTCTTCT TTGACAATGT CAGTCTCTGC TGTGACATTC 
     1261 AACAGCTTCA GACGCTGAAG AGTAACCTGC AGCTGCCCCT GCAGTTCCTG TCCAGGTGTC 
     1321 CGTCATGTTT TTATAACCTA ATGACCCTGT TTTGTGAGCT AACATGTAGC CCACACCAGA 
     1381 GTCAGTTTCT GAATGTGACA GCAACTGAGG ATTATTTTGA TCCTAAGACA CAGGAGAATA 
     1441 AAACAAATGT AAAGGAATTA GAGTACTTTG TCGGACAGAG CTTCGCGAAT GCGATGTACA 
     1501 ATGCCTGCCG TGATGTGGAG GCGCCTTCCA GTAACGAGAA GGCCTTAGGA CTCTTGTGTG 
     1561 GGAGGGATGC CCGTGCCTGC AATGCCACCA ACTGGATTGA GTACATGTTC AATAAAGACA 
     1621 ACGGACAAGC GCCATTTACC ATCATTCCTG TGTTTTCAGA TCTTTCAATC CTTGGGATGG 
     1681 AGCCCATGAG AAATGCCACC AAAGGCTGCA ATGAGTCTGT AGATGAGGTC ACGGGGCCAT 
     1741 GTAGCTGCCA GGACTGCTCC ATCGTCTGCG GCCCCAAGCC CCAGCCCCCA CCCCCTCCTA 
     1801 TGCCCTGGAG GATCTGGGGC TTGGATGCCA TGTATGTCAT CATGTGGGTC ACCTACGTGG 
     1861 CATTTCTGTT TGTGTTTTTT GGAGCACTGT TGGCAGTGTG GTGCCACAGA AGGCGGTACT 
     1921 TTGTGTCTGA GTACACTCCC ATTGACAGTA ACATCGCCTT TTCTGTGAAT AGCAGTGACA 
     1981 AAGGGGAAGC CTCATGCTGT GACCCACTTG GTGCAGCATT TGATGACTGT CTGAGGCGCA 
     2041 TGTTCACAAA GTGGGGGGCT TTCTGTGTCC GAAATCCCAC CTGCATCATT TTCTTCTCAT 
     2101 TGGCCTTCAT CACTGTGTGC TCTTCTGGCC TGGTATTTGT CCAGGTCACC ACCAATCCTG 
     2161 TAGAGCTCTG GTCAGCCCCT CACAGTCAGG CCCGCTTGGA AAAGGAGTAC TTTGACAAGC 
     2221 ACTTTGGGCC TTTCTTTCGC ACGGAGCAGC TTATCATCCA GGCCCCCAAC ACCAGTGTTC 
     2281 ATATCTACGA ACCGTACCCC GCAGGAGCCG ATGTGCCCTT CGGGCCTCCA TTGAACAAAG 
     2341 AGATTCTGCA CCAGGTTCTG GACTTACAGA TCGCCATTGA AAGCATCACC GCATCTTACA 
     2401 ACAATGAAAC CGTGACACTG CAGGACATCT GTGTGGCCCC CCTCTCTCCA TACAACAAGA 
     2461 ACTGCACCAT TATGAGTGTG TTAAATTACT TCCAGAACAG CCATGCGGTG CTGGACAGCC 
     2521 AAGTAGGCGA CGACTTCTAT ATCTACGCTG ATTACCACAC ACACTTTCTG TACTGTGTAC 
     2581 GGGCTCCCGC CTCCTTGAAT GATACGAGTT TGCTCCACGG TCCTTGCCTG GGTACATTTG 
     2641 GAGGACCGGT GTTCCCGTGG CTTGTGTTGG GTGGCTATGA TGATCAGAAC TACAACAATG 
     2701 CCACCGCGCT TGTGATCACC TTCCCCGTGA ATAATTACTA CAATGACACA GAGAGGCTCC 
     2761 AGAGGGCCTG GGCCTGGGAG AAAGAGTTTA TTAGTTTTGT GAAAAACTAC AAGAATCCAA 
     2821 ATCTGACCAT TTCTTTCACT GCTGAGCGAA GCATCGAAGA TGAGCTCAAT CGGGAAAGTA 
     2881 ACAGTGACGT GTTCACCGTC ATCATCAGCT ACGTCGTGAT GTTTCTGTAC ATTTCCCTCG 
     2941 CCCTGGGTCA CATCCAGAGC TGCAGCAGGC TCCTGGTGGA TTCTAAGATC TCGCTGGGCA 
     3001 TTGCGGGGAT CCTGATCGTG CTAAGCTCGG TGGCCTGCTC TCTGGGCATC TTCAGCTACA 
     3061 TGGGGATGCC GCTGACCCTC ATCGTCATTG AGGTCATCCC ATTCCTGGTG CTGGCTGTCG 
     3121 GGGTGGACAA CATCTTCATT CTAGTGCAGA CCTACCAGAG AGATGAGCGT CTTCAGGAGG 
     3181 AAACGCTGGA TCAGCAGCTG GGCAGGATCC TTGGAGAAGT GGCCCCGACC ATGTTCCTTT 
     3241 CATCCTTTTC TGAGACCTCA GCATTTTTCT TTGGGGCGCT GTCCTCGATG CCAGCTGTGC 
     3301 ACACCTTCTC TCTGTTTGCG GGAATGGCCG TCCTCATTGA CTTCCTCCTT CAGATTACCT 
     3361 GCTTTGTGAG CCTGTTGGGG TTAGATATTA AGAGGCAAGA GAAAAACCAT CTGGACATCC 
     3421 TGTGCTGTGT CAGAGGCGCT GACGACGGAC AAGGTAGCCA CGCCTCCGAA AGCTACCTGT 
     3481 TTCGCTTCTT CAAAAACTAC TTTGCCCCCC TTCTGCTGAA GGACTGGCTG AGGCCAATTG 
     3541 TGGTAGCGGT GTTTGTGGGC GTTCTGTCAT TCAGTGTTGC GGTGGTGAAC AAAGTAGACA 
     3601 TCGGGTTGGA TCAGTCTCTC TCAATGCCAA ACGATTCGTA TGTGATTGAC TATTTCAAAT 
     3661 CACTCGCTCA GTACCTGCAC TCGGGCCCAC CCGTGTACTT TGTCCTGGAG GAAGGCTATA 
     3721 ACTACAGTTC ACGCAAAGGG CAGAACATGG TGTGCGGCGG CATGGGCTGT GACAATGACT 
     3781 CCCTGGTGCA GCAGATATTT AACGCAGCTG AGCTGGACAC CTACACCCGA GTAGGCTTCG 
     3841 CCCCCTCGTC CTGGATCGAT GACTACTTTG ACTGGGTCTC GCCACAGTCG TCCTGCTGCA 
     3901 GACTCTACAA CGTCACTCAC CAGTTCTGCA ATGCTTCTGT GATGGACCCA ACCTGTGTCC 
     3961 GCTGCAGACC TCTGACTCCA GAGGGTAAAC AGAGGCCTCA GGGGAAAGAA TTCATGAAAT 
     4021 TCCTGCCCAT GTTCCTTTCT GATAACCCCA ACCCCAAGTG CGGCAAAGGG GGACATGCTG 
     4081 CTTACGGTTC AGCTGTTAAC ATTGTGGGAG ATGACACTTA CATTGGGGCC ACTTACTTCA 
     4141 TGACCTACCA CACCATACTT AAGACCTCCG CTGACTATAC TGATGCCATG AAGAAAGCTC 
     4201 GGCTAATAGC CAGTAACATC ACGGAAACCA TGCGTTCTAA GGGGAGTGAC TACCGCGTAT 
     4261 TCCCTTACAG TGTGTTCTAC GTCTTCTATG AACAGTACCT GACCATTATT GATGACACCA 
     4321 TCTTTAACCT CAGTGTGTCT CTGGGCTCCA TATTTCTGGT GACCTTGGTG GTTCTGGGCT 
     4381 GTGAGCTGTG GTCTGCGGTC ATCATGTGTA TCACCATAGC CATGATCCTG GTCAACATGT 
     4441 TCGGTGTCAT GTGGCTGTGG GGCATCAGTC TGAATGCGGT CTCCTTGGTC AACTTGGTGA 
     4501 TGAGCTGCGG CATTTCTGTG GAGTTCTGCA GCCATATAAC GAGAGCATTC ACCATGAGTA 
     4561 CCAAAGGAAG CCGAGTGAGC CGGGCGGAAG AGGCACTGGC CCACATGGGT AGTTCTGTAT 
     4621 TCAGTGGAAT CACACTTACG AAATTTGGAG GGATCGTGGT GTTAGCCTTT GCCAAATCTC 
     4681 AAATTTTTGA GATATTTTAC TTCAGGATGT ACTTAGCCAT GGTCTTACTC GGAGCCACTC 
     4741 ATGGACTAAT ATTTCTTCCC GTCTTACTCA GTTACATAGG GCCGTCGGTG AATAAAGCTA 
     4801 AAAGACACAC CACTTACGAG CGCTACAGAG GGACAGAGAG AGAACGGCTC CTCAATTTTA 
     4861 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4921 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4981 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5041 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5101 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5161 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5221 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5281 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5341 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5401 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5461 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5521 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5581 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5641 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5701 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5761 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5821 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5881 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5941 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6001 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6061 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6121 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6181 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6241 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6301 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6361 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6421 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6481 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6541 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6601 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6661 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6721 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6781 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6841 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6901 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6961 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7021 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7081 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7141 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7201 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7261 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7321 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7381 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7441 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7501 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7561 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7621 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7681 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7741 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7801 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7861 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7921 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8041 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8101 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8161 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8221 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8281 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8341 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8401 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8461 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8521 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8581 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8641 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8701 AACGCGAATT TT
//