LOCUS       MR211870                8676 bp    DNA     circular  SYN 
DEFINITION  Plcg2 (Myc-DDK-tagged) - Mouse phospholipase C, gamma 2 (Plcg2)
ACCESSION   NM_172285
VERSION     NM_172285.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4833..4924 
                     /label="Myc-DDK"  
     misc_feature    4954..5539 
                     /label="PolyA signal"  
     misc_feature    5011..5030 
                     /label="Restriction Enzyme"  
     misc_feature    5815..6487 
                     /label="ColE1"  
     misc_feature    6917..7711 
                     /label="Kan/Neo"  
     misc_feature    7746..8048 
                     /label="SV40 ori"  
     misc_feature    8134..8611 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4821 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4815 
                     /label="Plcg2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCACCATG GTCAACGTGG ACACCCTTCC AGAATATGAG AAGAGTCAGA 
     1081 TCAAGAGAGC GCTGGAGTTA GGGACGGTGA TGACCGTGTT CAACGCCCGC AAATCCACCC 
     1141 CAGAGCGGAG GACAGTACAG ATGATCATGG AGACTCGGCA GGTGGCATGG AGCAAGACCG 
     1201 CAGACAAGAT TGAAGGCTTC CTGGACATCA TGGAGATAAA GGAAATCCGT CCGGGGAAGA 
     1261 ACTCCAAGGA CTTTGAGCGA GCCAAGGCTG TCCGCCACAA GGCAGAATGT TGCTTCACCA 
     1321 TCCTCTACGG CACCCAGTTT GTCCTCAGCA CGCTCAGTTT GGCAACGGAC TCAAAGGAGG 
     1381 ATGCGGTGAA GTGGCTCTCT GGTTTGAAGA TCCTACACCA GGAAGCGATG AGTGCATCCA 
     1441 CCCCCACAAT GATTGAGAGT TGGCTGAGAA AACAGATTTA TTCAGTAGAT CAAACCCGAA 
     1501 GAAACAGCAT CAGCCTCCGG GAGCTGAAGA CCATCTTGCC CCTGGTCAAC TTCAAAGTGA 
     1561 GCGGCATCAA GTTTCTCAAG GACAAGCTGG TGGAAATCGG CGCACAGAAA GATGAGCTCA 
     1621 GCTTTGAACA GTTCCATCTC TTCTATAAGA AACTCATGTT TGACCAGCAA AAATCGATAC 
     1681 TTGACGAATT CAAAAAAGAC TCCTCCGTGT TCATCCTAGG AAACACAGAC CGGCCGGATG 
     1741 CCTCGGCTGT CTACCTGCAG GACTTCCAAA GGTTTCTTTT ACATGAACAG CAGGAGCTAT 
     1801 GGGCTCAGGA TCTGAACAAA GTCCGGGAGC GGATGACCAA GTTTATCGAC GACACAATGA 
     1861 GAGAGACCGC AGAGCCCTTC TTATTTGTGG ATGAGTTCCT CACGTATCTG TTCTCCCGGG 
     1921 AGAACAGCAT CTGGGACGAG AAGTACGATG CAGTGGACAT GCAGGACATG AACAACCCCT 
     1981 TGTCCCACTA CTGGATCTCC TCTTCCCACA ACACGTACCT CACTGGGGAC CAGCTGCGTA 
     2041 GTGAGTCCTC CACGGAAGCG TATATCCGCT GTCTGCGCGC TGGTTGCCGC TGCATTGAGC 
     2101 TGGACTGCTG GGACGGGCCT GATGGGAAGC CCATTATCTA CCACGGCTGG ACACGGACCA 
     2161 CCAAGATCAA GTTTGATGAT GTTGTTCAGG CCATCCGAGA CCACGCCTTT GTTACCTCCA 
     2221 GCTTCCCCGT CATCTTGTCT ATCGAGGAGC ACTGCAGTGT GGAGCAGCAA CGTCACATGG 
     2281 CCAAGGTGTT CAAGGAAGTG TTAGGAGACA TGCTGTTGAC GAAGCCCACA GAGGCCAGTG 
     2341 CTGACCAGCT GCCCTCGCCC AGTCAGCTTC GTGAGAAGAT CATTATCAAG CATAAGAAGC 
     2401 TGGGTCCCAA GGGTGATGTC GACGTCAACG TGGAGGACAA GAAAGATGAG CACAAGCCCC 
     2461 AGGGCGAGCT GTATATGTGG GACTCCATCG ACCAGAAATG GACGCGCCAC TACTGTGCCA 
     2521 TTGCTGACGC CAAGCTGTCC TTCGGTGATG ACATTGAACA GGCTGTGGAG GAGGAGCCGG 
     2581 TCCAGGACAC TCCCCCCACG GAGCTGCATT TTGGGGAGAA ATGGTTCCAC AAGAAGGTGG 
     2641 AGAGTAGGAC CAGTGCGGAG AAGCTGCTGC AAGAGTACTG TGCTGAGACC GGGGCCAAGG 
     2701 ACGGCACCTT CCTGGTGCGG GAGAGCGAGA CCTTCCCCAA TGACTACACA CTCTCCTTCT 
     2761 GGCGGTCTGG CCGGGTGCAG CACTGCCGGA TCCGGTCTAC TATGGAGAAT GGGGTCATGA 
     2821 AGTACTACCT GACCGACAAC CTCACGTTCA ACAGCATCTA CGCCCTCATC CAGCACTACC 
     2881 GTGAGGCACA CCTGCGCTGC GCGGAGTTCG AGCTGCGGCT CACAGACCCG GTGCCCAACC 
     2941 CCAACCCACA CGAGTCCAAG CCGTGGTACT ATGACAGTCT GAGCAGGGGG GAAGCGGAGG 
     3001 ACATGCTGAT GCGGATCCCC CGAGATGGAG CCTTCCTCAT CCGGAAGAGG GAGGGGACCA 
     3061 ACTCCTACGC CATCACCTTC AGGGCCAGGG GCAAGGTGAA ACATTGTCGC ATCAACCGGG 
     3121 ACGGACGCCA CTTCGTGCTG GGCACCTCTG CCTACTTTGA GAGCCTAGTG GAGCTTGTCA 
     3181 GCTACTACGA GAAGCACGCG CTCTATCGCA AGATGAGGCT TCGATACCCC GTGACCCCGG 
     3241 AGCTCCTGGA GCGATATAAT ATGGAAAGAG ACATCAACTC TCTTTATGAC GTCAGCAGGA 
     3301 TGTATGTGGA CCCAAGTGAG ATCAACCCTT CCATGCCTCA GAGGACCGTG AAAGCGCTCT 
     3361 ATGACTACAA AGCCAAGCGC AGCGACGAGC TGACCTTCTG CCGCGGTGCC CTCATCCACA 
     3421 ATGTCTCCAA GGAGCCCGGG GGATGGTGGA AAGGGGACTA CGGGACCCGG ATCCAGCAGT 
     3481 ACTTCCCGTC CAACTATGTC GAGGATATCT CGGCAGGCGA TGCTGAGGAG ATGGAAAAGC 
     3541 AGATTATTGA AGACAATCCC CTCGGGTCTC TCTGCAAAGG CATATTGGAT CTTAATACCT 
     3601 ACAACGTTGT GAAGGCCCCC CAGGGGAAAA ACCAGAAAGC CTTCGTCTTT ATCCTGGAGC 
     3661 CTAAGAAACA GGGAGATCCT CCCGTGGAGT TCGCGACCGA CCGTGTGGAG GAGCTGTTTG 
     3721 AGTGGTTTCA GAGCATACGG GAGATCACTT GGAAGATGGA CACCAAGGAG AACAACATGA 
     3781 AGTACTGGGA GAGGAACCAG TCCATCGCCA TTGAGCTCTC GGACTTGGTT GTATACTGCA 
     3841 AGCCAACTAG CAAAACCAAG GACCATTTGG AAAATCCCGA CTTCCGAGAA ATCCGCTCTT 
     3901 TTGTGGAGAC GAAGGCAGAC AGCATTGTCA GGCAAAAACC TGTCGATCTA TTGCGATACA 
     3961 ATCAGAAGGG CCTGACCCGA GTCTACCCCA AGGGACAGAG AGTTGACTCT TCGAACTATG 
     4021 ACCCCTTCCG TCTGTGGCTG TGCGGCTCCC AGATGGTGGC ACTCAATTTC CAGACTCCAG 
     4081 ACAAATACAT GCAGATGAAC CACGCGCTGT TTTCGCTCAA TGGGCGAACA GGCTACGTCC 
     4141 TGCAGCCTGA GAGCATGCGC TCTGAGAAGT ATGATCCGAT GCCCCTGGAG TCCCAGAGGA 
     4201 AGATCCTGAT GACACTCACT GTCAAGGTTC TTGGTGCACG CCACCTCCCT AAACTAGGGC 
     4261 GGAGTATTGC CTGTCCTTTT GTGGAGGTGG AAATCTGCGG GGCTGAGTAT GACAGCAACA 
     4321 AATTCAAGAC GACGGTTGTG AATGACAATG GCCTCAGCCC TGTCTGGGCT CCAACTCAGG 
     4381 AGAAGGTGAC ATTTGAGATC TATGATCCAA ACCTTGCCTT CCTACGCTTT GTGGTCTACG 
     4441 AAGAAGACAT GTTCAGTGAT CCCAACTTCC TGGCTCATGC CACGTACCCC ATTAAAGGCA 
     4501 TCAAATCCGG GTTTAGATCA GTCCCTCTGA AGAATGGGTA CAGTGAAGAC ATCGAGCTGG 
     4561 CATCCCTCCT GGTTTTCTGT GAGATGAGGC CAGTCCTGGA GAGTGAAGAG GAACTCTATT 
     4621 CCTCCTGTCG CCAACTGCGG AGGCGGCAAG AAGAGCTCAA CAACCAGCTC TTCCTTTACG 
     4681 ACACGCACCA GAACCTGCGC GGAGCCAACC GTGATGCCCT GGTGAAGGAG TTCAATGTTA 
     4741 ACGAGAATCA GCTGCAGCTG TACCAGGAGA AGTGCAACCG GAGGCTGAGA GAGAAGAGAG 
     4801 TAAGCAACAG CAGGTTCTAC TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//