LOCUS       MR211869                8673 bp    DNA     circular  SYN 
DEFINITION  Dctn1 (Myc-DDK-tagged) - Mouse dynactin 1 (cDNA clone MGC:86152 IMAGE:6849060)
ACCESSION   BC066061
VERSION     BC066061.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4830..4921 
                     /label="Myc-DDK"  
     misc_feature    4951..5536 
                     /label="PolyA signal"  
     misc_feature    5008..5027 
                     /label="Restriction Enzyme"  
     misc_feature    5812..6484 
                     /label="ColE1"  
     misc_feature    6914..7708 
                     /label="Kan/Neo"  
     misc_feature    7743..8045 
                     /label="SV40 ori"  
     misc_feature    8131..8608 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4812..4818 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4812 
                     /label="Dctn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTACGGAG GCAAGCGCCC GGCCCCTGCG GGTTGGCTCC CGCGTGGAGG 
     1081 TGATTGGGAA GGGCCACCGA GGCACTGTGG CCTATGTTGG AGCCACACTC TTTGCCACTG 
     1141 GCAAATGGGT GGGCGTGATT CTGGATGAAG CAAAAGGCAA AAATGATGGC ACTGTCCAGG 
     1201 GAAGGAAGTA TTTCACATGT GATGAAGGCC ACGGCATCTT TGTACGCCAG TCCCAGATCC 
     1261 AAGTATTTGA AGATGGAGCA GATACTACTT CCCTAGAGAC TCCTGATTCT TCTGCTTCAA 
     1321 AGGTCCTCAA GAGAGAGGGA GCCGATGCAG CTGCAAAGAC CAGCAAACTG CGGGGACTGA 
     1381 AGCCTAAGAA GGCACCGACA GCCCGAAAGA CCACAACTCG ACGGCCCAAG CCTACTCGCC 
     1441 CAGCCAGCAC TGGGGTGGCT GGGCCCAGTA GCTCCCTTGG CCCCTCTGGC TCAGCGTCAG 
     1501 CCGGGGAACT AAGCAGCAGT GAGCCCAGCA CCCCAGCTCA GACTCCGCTG GCAGCACCCA 
     1561 TCATCCCCAC ACCGGCCCTC ACCTCTCCTG GAGCAGCACC CCCACTTCCA TCTCCCTCTA 
     1621 AGGAAGAGGA AGGGCTGAGG GCTCAGGTAC GGGACCTGGA GGAGAAGCTG GAGACCCTGC 
     1681 GCCTAAAACG CTCAGAAGAC AAAGCAAAGC TGAAAGAGCT GGAGAAGCAC AAGATCCAGC 
     1741 TGGAGCAGGT GCAGGAATGG AAGAGCAAAA TGCAGGAGCA GCAGGCAGAC CTGCAGCGGC 
     1801 GCCTCAAGGA GGCTCGGAAG GAAGCCAAGG AGGCGCTAGA GGCAAAGGAA CGCTACATGG 
     1861 AGGAGATGGC CGACACAGCC GACGCTATCG AGATGGCCAC TCTGGACAAG GAGATGGCTG 
     1921 AAGAGCGCGC TGAGTCTCTG CAGCAAGAGG TGGAGGCACT GAAGGAACGG GTAGACGAGC 
     1981 TCACCACAGA CCTGGAGATT CTCAAGGCTG AAATCGAAGA GAAAGGCTCT GATGGGGCCG 
     2041 CATCAAGCTA CCAGCTCAAG CAGCTGGAGG AGCAGAATGC CCGCCTGAAG GATGCCCTGG 
     2101 TGAGGATGCG AGACCTCTCT TCCTCAGAGA AGCAGGAGCA CGTGAAGCTG CAGAAACTCA 
     2161 TGGAAAAGAA AAACCAGGAG CTGGAGGTCG TGCGGCAGCA GCGCGAGCGT CTTCAGGAGG 
     2221 AGCTGAGCCA GGCTGAGAGC ACCATCGATG AGCTCAAAGA GCAGGTGGAC GCCGCTCTGG 
     2281 GAGCCGAGGA GATGGTGGAG ATGCTGACCG ACCGGAACCT GAATCTAGAG GAGAAAGTGC 
     2341 GGGAGTTACG GGAGACTGTG GGGGACTTGG AAGCCATGAA CGAGATGAAC GATGAGCTGC 
     2401 AGGAGAACGC ACGGGAGACG GAGCTGGAAC TCCGAGAGCA GCTGGACATG GCGGGCGCCC 
     2461 GAGTGAGGGA AGCGCAGAAG CGAGTGGAAG CCGCCCAGGA GACAGTCGCC GACTACCAGC 
     2521 AGACCATCAA GAAGTACCGC CAGTTGACTG CCCACCTACA GGATGTCAAT CGGGAGCTGA 
     2581 CAAACCAGCA GGAAGCGTCT GTAGAGAGGC AGCAGCAGCC GCCGCCAGAG ACTTTTGATT 
     2641 TCAAAATCAA GTTTGCTGAG ACCAAGGCTC ATGCCAAGGC CATTGAGATG GAGTTGAGAC 
     2701 AGATGGAAGT TGCCCAGGCC AACCGGCACA TGTCCCTGCT GACAGCCTTT ATGCCTGACA 
     2761 GCTTCCTTCG GCCAGGTGGA GACCACGACT GTGTCCTGGT GCTGCTGCTC ATGCCCCGAC 
     2821 TCATTTGCAA GGCAGAGCTC ATCCGGAAGC AGGCCCAGGA GAAGTTTGAC CTGAGCGAGA 
     2881 ACTGTTCGGA GCGGCCCGGG CTGCGGGGAG CTGCCGGGGA GCAGCTGAGC TTTGCTGCTG 
     2941 GACTGGTGTA CTCGCTGAGT CTGCTGCAGG CCACGCTGCA CCGCTATGAG CATGCCCTCT 
     3001 CTCAGTGCAG TGTGGACGTG TATAAGAAGG TCGGCAGCCT GTACCCCGAG ATGAGCGCCC 
     3061 ACGAGCGCTC CTTAGATTTC CTCATTGAGC TGCTGCACAA GGATCAGCTG GATGAGACTG 
     3121 TCAACGTGGA GCCCCTCACC AAGGCCATCA AGTATTACCA GCATCTGTAC AGCATCCACC 
     3181 TCGCTGAACA ACCCGAGGAC TCCACCATGC AGCTGGCTGA CCACATCAAG TTCACCCAGA 
     3241 GTGCCCTGGA CTGCATGGGC GTGGAGGTGG GGCGGCTGCG TGCCTTCTTG CAGGGTGGGC 
     3301 AGGAGGCAAC AGATATTGCC CTTCTTCTCC GAGACCTGGA AACATCATGT AGTGACACCC 
     3361 GTCAGTTCTG CAAGAAGATC CGAAGGCGGA TGCCGGGGAC GGATGCTCCT GGGATCCCAG 
     3421 CAGCGCTGGC CTTTGGCTCA CAGGTATCCG ACACACTCCT GGACTGCAGG AAGCACTTGA 
     3481 CGTGGGTGGT AGCTGTTCTG CAGGAGGTGG CAGCTGCAGC CGCCCAGCTT ATTGCCCCCT 
     3541 TGGCAGAGAA CGAGGGGCTG CCTGTGGCTG CACTGGAGGA GCTGGCCTTC AAAGCAAGCG 
     3601 AGCAGATCTA CGGGAGCCCC TCCAGCAGCC CCTATGAGTG TCTACGCCAG TCATGCACCA 
     3661 TCCTCATCAG CACGATGAAC AAGCTGGCCA CAGCCATGCA AGAAGGCGAG TATGACGCAG 
     3721 AGCGACCCCC GAGCAAGCCT CCTCCGGTTG AACTTCGGGC TGCAGCCCTG CGTGCAGAGA 
     3781 TCACAGATGC TGAAGGTCTG GGTTTGAAGC TTGAGGATCG AGAGACAGTT ATCAAGGAGT 
     3841 TAAAGAAGTC ACTCAAGATT AAGGGAGAGG AGCTGAGTGA GGCCAACGTG CGGCTCAGCC 
     3901 TCCTGGAGAA GAAGTTGGAC AGCGCTGCCA AGGATGCAGA CGAGCGAATC GAGAAAGTTC 
     3961 AGACACGGCT GGACGAGACT CAGACCCTGC TGCGGAAGAA GGAGAAAGAC TTTGAGGAGA 
     4021 CAATGGACGC ACTCCAGGCT GACATCGACC AGCTGGAGGC AGAGAAGGCA GAGCTCAAGC 
     4081 AGCGCCTGAA CAGCCAGTCC AAGCGCACAA TCGAGGGGCT CCGGGGCCCC CCTCCGTCAG 
     4141 GCATCGCTAC CCTGGTCTCT GGCATCGCTG GTGAGGAACC ACAGCGAGGG GGCGCTCCTG 
     4201 GGCAGGCTCC GGGCGCCTTG CCAGGCCCGG GGCTGGTGAA GGACTCCCCA CTGCTGCTTC 
     4261 AGCAGATCTC TGCTATGAGG CTACACATCT CTCAGCTCCA GCATGAGAAC AGCATCCTCA 
     4321 GAGGAGCCCA GATGAAGGCG TCCTTGGCAG CTCTGCCCCC TCTGCATGTT GCAAAGCTTT 
     4381 CCCTCCCACC CCATGAGGGC CCCGGTGGTA ACCTAGTGGC TGGGGCACTG TACCGCAAGA 
     4441 CCAGCCAGCT CCTGGAGAAA CTAAACCAGC TGAGTACCCA CACCCACGTG GTGGATATCA 
     4501 CTCGGAGCAG CCCAGCTGCC AAGAGCCCGT CAGCTCAGCT TATGGAACAA GTGGCTCAGC 
     4561 TCAAGTCCCT GAGTGACACC ATTGAGAAGC TCAAGGATGA GGTCCTCAAG GAGACAGTGA 
     4621 CTCAGCGCCC TGGAGCCACT GTGCCCACCG ACTTTGCCAC TTTCCCTTCA TCTGCCTTCC 
     4681 TCAGGGCCAA GGAAGAGCAG CAAGATGACA CAGTCTACAT GGGCAAAGTG ACCTTTTCAT 
     4741 GCGCGGCAGG CCTAGGACAG CGACACCGCC TGGTGCTGAC CCAGGAGCAG CTGCACCAGC 
     4801 TTCACAGTCG CCTCATCTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4861 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4921 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4981 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5041 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5101 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5161 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5221 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5281 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5341 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5401 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5461 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5521 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5581 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5641 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5701 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5761 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5821 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5881 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5941 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6001 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6061 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6121 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6181 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6241 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6301 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6361 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6421 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6481 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6541 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6601 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6661 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6721 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6781 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6841 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6901 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6961 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7021 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7081 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7141 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7201 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7261 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7321 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7381 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7441 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7501 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7561 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7621 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7681 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7741 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7801 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7861 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7921 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8101 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8161 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8221 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8281 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8341 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8401 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8461 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8521 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8581 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8641 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//