LOCUS       MR211867                8670 bp    DNA     circular  SYN 
DEFINITION  Vars (Myc-DDK-tagged) - Mouse valyl-tRNA synthetase (Vars), nuclear gene encoding mitochondrial protein
ACCESSION   NM_011690
VERSION     NM_011690.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4827..4918 
                     /label="Myc-DDK"  
     misc_feature    4948..5533 
                     /label="PolyA signal"  
     misc_feature    5005..5024 
                     /label="Restriction Enzyme"  
     misc_feature    5809..6481 
                     /label="ColE1"  
     misc_feature    6911..7705 
                     /label="Kan/Neo"  
     misc_feature    7740..8042 
                     /label="SV40 ori"  
     misc_feature    8128..8605 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4809..4815 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4809 
                     /label="Vars"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCATCCTC TACGTCTCTC CTCACCCCGA CGCCTTCCCC AGCCTCCGAG 
     1081 CCCTCATCGC TGCCCGATAC GGGGAAGCTG GTGACGGTCC CGGGTGGGGA GGCCCTCACC 
     1141 CCCGCATCTG CTTGCAGCCT CCCCCGAGCA GCCGGACTCC ATTCCCTCCG CCTCGCCTGC 
     1201 CCGCCCTGGA GCAGGGACCT GGCGGCCTGT GGGTGTGGGG GGCCCCGGCT GTAGCTCAGC 
     1261 TGTTGTGGCC TGCAGGCCTG GGGGGACCCG GGGGCAGCCG GGCAGCTGTC CTGGTCCAGC 
     1321 AGTGGGTCAG TTATGCTGAC ACGGAGCTCA TACCCGCTGC CTGCGGGGCG ACCCTGCCTG 
     1381 CCCTGGGACT TCGAGGTCCT GGTCAGGATC CCCAGGCTGC CCTTGGGGCC CTGGGCAAGG 
     1441 CCTTGAACCC CTTGGAGGAC TGGCTGCGGC TGCACACCTA CCTGGCTGGA GATGCCCCCA 
     1501 CTCTGGCTGA CTTAGCTGCT GTGACAGCCT TACTGCTGCC TTTCCGATAC GTTCTGGACC 
     1561 CTTCTGCCCG CCGGATCTGG GGCAATGTGA CTCGCTGGTT TAACACTTGT GTCCGGCAAC 
     1621 CGGAATTCCG GGCTGTGCTG GGAGAAGTGG CCCTGTACTC GGGGGCCAGG TCTGTCACTC 
     1681 AACAGCCAGG CTCTGAGGTC ATCGCACCCC AAAAAACACC CGCGCAGCTC AAAAAAGAGG 
     1741 CAAAGAAACG GGAGAAACTA GAGAAATTCC AGCAGAAGCA GAAGACCCAG CAGCAGCCCC 
     1801 CGCACGGAGA GAAGAAACCA AAACCAGAGA AGAAGGAGAA ACGGGACCCT GGGGTCATTA 
     1861 CCTATGACCT CCCTACCCCA CCGGGGGAGA AGAAAGATGT AAGTGGCGCC ATGCCCGACT 
     1921 CCTACAGCCC TCAGTATGTG GAGGCTGCCT GGTATCCATG GTGGGAGCGG CAGGGCTTCT 
     1981 TCAAGCCAGA GTACGGGCGT CCTAGTGTGT CAGCACCAAA TCCCCGGGGT GTCTTCATGA 
     2041 TGTGCATCCC ACCCCCCAAC GTGACAGGCT CCCTGCACCT GGGCCACGCA CTCACCAACG 
     2101 CCATCCAGGA CTCCCTGACT CGATGGCACC GCATGCGTGG GGAGACCACC CTATGGAACC 
     2161 CAGGCTGTGA CCATGCAGGC ATTGCCACCC AGGTGGTGGT GGAAAAGAAG CTCTGGAAAG 
     2221 AGCGGGGTCT GAACCGGCAC CAGCTGGGCC GCGAGGCCTT TCTTGAGGAG GTCTGGAAGT 
     2281 GGAAAGCCGA GAAGGGTGAC AGGATTTACC ACCAATTAAA GAAACTTGGC AGCTCCTTGG 
     2341 ACTGGGATCG AGCCTGCTTC ACCATGGATC CTAAATTGTC AGCAACTGTG ACAGAGGCCT 
     2401 TTGTTAGGCT CCACGAAGAA GGGGTCATCT ACCGTAGCAC CCGCCTGGTC AACTGGTCCT 
     2461 GCACCCTCAA CTCAGCCATT TCTGACATTG AGGTGGATAA GAAGGAGCTG ACAGGCCGCA 
     2521 CCCTGCTTCC TGTGCCTGGC TACAAGGAGA AGGTGGAGTT TGGGGTCCTT GTGTCCTTTG 
     2581 CCTACAAGGT CCAAGGCTCA GACAGCGACG AGGAGGTGGT GGTGGCAACA ACCCGGATTG 
     2641 AGACCATGCT GGGAGACGTG GCTGTAGCTG TGCACCCCAA GGATCCCAGA TACCAGCACT 
     2701 TAAAGGGAAA GTGTGTCGTC CACCCATTCT TGTCCCGGAG CCTTCCCATC GTCTTCGATG 
     2761 ACTTTGTAGA CATGGAGTTT GGCACAGGTG CCGTGAAGAT CACCCCGGCA CATGACCAAA 
     2821 ATGACTATGA GGTTGGGCAG CGACACAGGC TCGAGGCCAT TAGCATCATG GACTCAAAGG 
     2881 GGGCCCTCAT CAATGTGCCT CCACCTTTCC TGGGCCTGCC CAGGTTTGAG GCCAGGAAGG 
     2941 CTGTGCTGGC GGCACTGAAG GAGCGGGGCC TGTTCCGTGG AGTCAAGGAC AACCCCATGG 
     3001 TGGTGCCGCT TTGCAACCGC TCCAAGGATG TGGTGGAGCC TCTGTTAAGG CCACAATGGT 
     3061 ACGTGCGCTG TGGGGAGATG GCTCAGGCTG CCAGTGCTGC TGTGACCCGG GGTGACCTCC 
     3121 GTATACTGCC TGAGGCCCAT CAACGGACGT GGCATTCTTG GATGGACAAC ATCAGAGACT 
     3181 GGTGCATCTC TCGGCAGCTG TGGTGGGGCC ACCGAATCCC TGCCTACTTT ATCACCGTCC 
     3241 ATGACCCGGC AGTACCCCCT GGGGAGGACC CTGATGGGCG GTACTGGGTA AGCGGACGCA 
     3301 CTGAAGCAGA GGCCCGAGAG AAGGCAGCAC GGGAGTTTGG AGTGTCCCCT GACAAGATCA 
     3361 GCCTTCAGCA AGATGAGGAT GTGTTGGACA CCTGGTTCTC CTCCGGTCTC TTTCCCTTCT 
     3421 CCATCTTTGG CTGGCCCAAT CAGTCAGAAG ACCTCAGTGT GTTCTACCCT GGGACCCTGT 
     3481 TGGAGACAGG CCATGATATC CTCTTCTTCT GGGTGGCCCG GATGGTCATG CTCGGCCTGA 
     3541 AGCTCACTGG GAAGCTGCCC TTCAGAGAGG TCTATCTCCA TGCAATCGTG CGTGATGCTC 
     3601 ATGGCAGGAA GATGAGCAAG TCTCTTGGCA ATGTCATCGA CCCCCTGGAT GTCATCCATG 
     3661 GAGTTTCCTT GCAGGGCCTC TATGACCAAC TACTGAACAG CAACCTGGAT CCCAGTGAGG 
     3721 TGGAGAAAGC CAAAGAAGGA CAGAAGGCCG ACTTTCCAGC AGGGATTCCC GAGTGTGGCA 
     3781 CTGATGCCCT ACGCTTTGGA CTCTGTGCCT ACACATCCCA AGGTCGAGAC ATCAACCTGG 
     3841 ATGTGAACAG GATCCTGGGG TACCGTCACT TCTGCAACAA ACTCTGGAAT GCTACCAAGT 
     3901 TTGCCCTGCG CGGCCTTGGG AAGGGCTTTG TGCCCTCAGC AACCTCCAAG CCTGAAGGGC 
     3961 ACGAGAGCCT GGTGGACCGC TGGATCCGCA GCCGCCTAAC TGAGGCCGTG AGGCTCAGCA 
     4021 ATGAAGGCTT CCAGGCTTAC GATTTCCCGG CCATCACCAC CGCCCAGTAC AGCTTTTGGC 
     4081 TCTATGAGCT CTGTGATGTC TATTTGGAGT GTCTAAAACC TGTGCTGAAT GGAGTGGACC 
     4141 AGGTGGCGGC AGAGTGTGCT CGGCAGACCC TCTACACCTG CCTGGATGTT GGCCTGCGGC 
     4201 TGCTCTCGCC CTTCATGCCC TTTGTCACAG AGGAGCTATT CCAGAGACTG CCCCGGCGGA 
     4261 CACCAAAAGC GCCTGCTAGC CTCTGTGTCA CCCCGTACCC AGAGCCCTCA GAGTGCTCCT 
     4321 GGAAGGACCC TGAAGCTGAA GCTGCTCTTG AACTAGCTCT GAGCATCACT CGAGCTGTGC 
     4381 GCTCCCTGCG TGCTGACTAC AACCTGACCC GCACCAGGCC CGACTGTTTC TTGGAAGTAG 
     4441 CCGATGAGGC CACAGGTGCC TTGGCCTCGG CAGTGTCGGG CTACGTGCAG GCTCTGGCCA 
     4501 GTGCGGGCGT GGTGGCTGTC CTCGCCCTGG GTGCTCCTGC ACCGCAGGGC TGCGCTGTAG 
     4561 CTGTGGCTTC TGACCGCTGC TCCATCCACC TGCAGCTGCA GGGGCTAGTG GACCCAGCCC 
     4621 GGGAGTTGGG CAAGCTGCAG GCCAAGCGAA GTGAGGCACA GCGGCAGGCT CAGCGGCTGC 
     4681 AGGAGCGCCG TGCTGCCTCC AGCTACTCAG CAAAGGTGCC CCTTGAGGTC CAGGAGGCAG 
     4741 ATGAAGCGAA GTTGCAACAG ACAGAGGCGG AGCTCAGGAA GGTGGATGAG GCCATCGCCC 
     4801 TGTTCCAGAA GATGCTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4861 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4921 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4981 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5041 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5101 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5161 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5221 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5281 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5341 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5401 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5461 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5521 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5581 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5641 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5701 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5761 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5821 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5881 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5941 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6001 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6061 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6121 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6181 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6241 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6301 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6361 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6421 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6481 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6541 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6601 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6661 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6721 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6781 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6841 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6901 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6961 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7021 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7081 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7141 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7201 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7261 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7321 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7381 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7441 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7501 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7561 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7621 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7681 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7741 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7801 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7861 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7921 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7981 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8041 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8101 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8161 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8221 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8281 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8341 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8401 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8461 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8521 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8581 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8641 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//