LOCUS       MR211858                8649 bp    DNA     circular  SYN 
DEFINITION  Ppfia2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (Ppfia2), transcript variant 2
ACCESSION   NM_177373
VERSION     NM_177373.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="Ppfia2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTGAA GTGATGCCCA CGATTAACGA GGACACCCCA ATGAGCCAAC 
     1081 GGGGTTCCCA AAGCAGTGGC TCCGACTCGG ACTCCCATTT TGAGCAACTC ATGGTCAATA 
     1141 TGCTGGATGA AAGGGACCGC CTACTGGACA CACTCCGAGA GACCCAGGAG AGCCTCTCAC 
     1201 TTGCCCAGCA AAGGCTCCAG GATGTCATCT ATGACAGAGA TTCACTCCAG AGACAGCTGA 
     1261 ATTCAGCCCT ACCTCAGGAC ATCGAATCCC TAACAGGAGG GCTGACTGGT TCTAAGGGGG 
     1321 CTGATCCACC GGAGTTTGCT GCACTGACTA AAGAGTTGAA TGCTTGCCGT GAACAACTTC 
     1381 TAGAAAAGGA AGAAGAAATC TCAGAGCTGA AAGCCGAAAG AAACAACACA AGACTGTTAC 
     1441 TGGAACACTT GGAGTGCCTT GTGTCCCGAC ATGAACGGTC ACTAAGAATG ACGGTGGTCA 
     1501 AGCGGCAAGC CCAGTCTCCC TCTGGAGTGT CCAGTGAAGT GGAGGTTCTC AAGGCACTGA 
     1561 AATCTTTGTT TGAACACCAC AAAGCCTTGG ATGAAAAGGT AAGGGAGCGA CTGAGGGTTT 
     1621 CTTTAGAAAG AGTCTCTGCA CTGGAAGAGG AACTAGCTGC AGCTAATCAG GAGATTGTTG 
     1681 CCTTGCGTGA GCAAAATGTT CATATTCAAA GGAAAATGGT ATCAAGTGAG GGGTCCACGG 
     1741 AGTCAGAGCA TCTTGAAGGA ATGGAAGCAG GCCAGAAAGT TCATGAAAAG CGTCTATCCA 
     1801 ATGGCTCCAT AGATTCGACA GATGACACCA GCCAAATCGT CGAGCTGCAA GAATTGCTTG 
     1861 AGAAGCAAAA CTATGAAATG GCCCAAATGA AAGAACGCTT AACAGCTCTC TCTTCCCGGG 
     1921 TGGGAGAGGT GGAACAAGAA GCAGAGACAG CAAGAAAGGA CCTCATTAAA ACAGAAGAAA 
     1981 TGAACACAAA ATATCAAAGG GACATCCGAG AGGCCATGGC TCAGAAGGAA GATATGGAAG 
     2041 AAAGAATCAC AACTCTGGAG AAGCGTTACC TGAGTGCTCA GAGAGAATCT ACCTCCATTC 
     2101 ATGATATGAA TGATAAACTA GAAAATGAAT TGGCGAACAA GGAAGCCATC TTGCGGCAGA 
     2161 TGGAAGAAAA AAACAGGCAA TTGCAAGAGC GTCTTGAACT GGCTGAGCAA AAGTTACAGC 
     2221 AGACCATGAG AAAAGCTGAA ACTTTACCTG AAGTAGAGGC TGAACTTGCT CAGAGAATTG 
     2281 CAGCCCTAAC AAAGGCTGAA GAGAGGCATG GAAATATTGA AGAACGTATG AGACACCTCG 
     2341 AGGGCCAACT TGAAGAGAAG AATCAAGAAC TTCAAAGAGC TAGACAAAGG GAGAAAATGA 
     2401 ATGAAGAGCA TAATAAGAGA TTATCGGATA CGGTGGACAG GCTTCTGACA GAATCCAATG 
     2461 AGCGCCTGCA ACTACACCTG AAGGAAAGGA TGGCTGCTTT GGAAGAGAAG AATGTTTTGA 
     2521 TTCAAGAATC AGAAAACTTC AGAAAAAATC TTGAAGAGTC CTTACATGAT AAGGAAAGAT 
     2581 TAGCAGAAGA AATCGAGAAG CTGAGGTCTG AACTTGATCA AATGAAAATG AGAACAGGTT 
     2641 CTCTAATTGA ACCCACTATA TCAAGAACTC ATATAGACAC CTCCACTGAG TTGCGGTATT 
     2701 CAGTTGGGTC CCTTGTAGAC AGCCAGTCTG ATTACAGAAC CACTAAAGTA ATACGAAGAC 
     2761 CAAGGAGAGG CCGCATGGGT GTGCGAAGAG ATGAACCAAA GGTGAAATCC CTTGGAGATC 
     2821 ATGAGTGGAA CAGAACTCAG CAAATTGGAG TACTAGGAAG CCACCCTTTC GAAAGTGACA 
     2881 CTGAAATGTC TGATATTGAT GATGATGACA GAGAAACAAT TTTTAGCTCA ATGGATCTTC 
     2941 TCTCTCCAAG TGGCCATTCT GATGCCCAGA CTCTAGCTAT GATGCTTCAA GAACAATTGG 
     3001 ATGCAATCAA TAAAGAAATA AGACTAATTC AAGAAGAAAA GGAATCGACA GAATTGCGTG 
     3061 CTGAAGAAAT TGAGAATAGA GTGGCTAGTG TAAGCTTAGA AGGGCTGAAT TTGGCGAGGG 
     3121 TCCATCCAGG TACCTCCATC ACTGCCTCTG TTACAGCTTC ATCACTGGCC AGTTCATCTC 
     3181 CCCCAAGTGG ACACTCAACG CCAAAGCTTA CACCAAGAAG CCCTGCCAGG GAAATGGACC 
     3241 GCATGGGAGT GATGACCCTG CCAAGTGATC TAAGGAAACA TCGGAGAAAG ATTGCAGTGG 
     3301 TGGAAGAAGA TGGACGGGAG GATAAAGCCA CAATTAAATG CGAGACTTCT CCTCCCCCAA 
     3361 CACCCAGAGC CGTCAGAATG ACTCACACCT TACCATCTTC CTACCACAAT GATGCCCGGA 
     3421 GTTTATCTGC CTCTCTTGAG CCAGACAGTC TTGGGCTTGG CAGTGCCAAT AGCAGCCAAG 
     3481 ATTCTCTCCA CAAAGCCCCC AAGAAGAAAG GAATCAAGTC TTCAATTGGG CGCTTATTTG 
     3541 GGAAAAAAGA GAAGGCTCGA CTCGGGCAGC TTCGAGGCTT CATGGAGACA GAAGCTGCAG 
     3601 CACAGGAATC CCTGGGCTTA GGCAAACTTG GAACTCAAGC TGAAAAGGAC AGAAGGCTGA 
     3661 AGAAAAAGCA TGAACTTCTT GAAGAAGCTC GTAGAAAAGG ATTACCTTTT GCCCAGTGGG 
     3721 ATGGACCCAC CGTGGTTGCG TGGCTGGAGC TCTGGCTGGG AATGCCAGCT TGGTACGTGG 
     3781 CAGCATGCAG AGCCAACGTG AAAAGTGGTG CCATCATGTC AGCGTTATCG GACACTGAAA 
     3841 TCCAAAGGGA GATTGGAATC AGCAACCCTC TGCACCGCTT AAAGCTCAGG CTAGCTATCC 
     3901 AAGAGATGGT TTCTCTCACC AGCCCTTCAG CGCCTCCGAC ATCGCGTACT CCTTCAGGCA 
     3961 ATGTGTGGGT GACCCATGAA GAAATGGAAA ATCTGACAGC TCCAGCAAAA ACGAAAGAAT 
     4021 CTGAGGAAGG AAGCTGGGCC CAGTGTCCGG TTTTTCTACA GACCCTGGCT TATGGAGATA 
     4081 TGAACCACGA GTGGATTGGA AATGAATGGC TTCCCAGCCT GGGGTTACCT CAATACAGAA 
     4141 GTTACTTTAT GGAATGCTTG GTTGATGCAA GAATGTTAGA CCACCTCACA AAAAAGGATC 
     4201 TTCGTGTCCA TTTAAAAATG GTGGATAGTT TCCATCGAAC AAGTTTACAG TATGGAATTA 
     4261 TGTGCTTGAA AAGATTGAAT TATGACAGAA AAGAACTAGA GAGAAGACGA GAAGCAAGTC 
     4321 AACATGAAAT AAAAGATGTT TTAGTGTGGA GCAATGATCG AGTTATTCGC TGGATACAAG 
     4381 CAATTGGACT TCGGGAATAT GCAAACAACA TTCTTGAGAG TGGCGTGCAT GGCTCACTTA 
     4441 TAGCCCTGGA TGAGAACTTT GACTACAGCA GCTTGGCTCT ATTGTTGCAG ATTCCAACAC 
     4501 AGAACACCCA GGCAAGGCAG ATTCTTGAAC GTGAGTACAA CAACCTCTTG GCCCTGGGAA 
     4561 CTGAGCGGAG ACTGGATGAA AGTGACGACA AGAATTTCAG ACGGGGATCA ACCTGGCGAA 
     4621 GGCAGTTCCC TCCTCGTGAA GTTCATGGAA TCAGCATGAT GCCTGGGTCC TCAGAAACAT 
     4681 TACCAGCTGG ATTCAGGTTG ACCACAACGT CTGGGCAGTC CAGGAAAATG ACGACAGACG 
     4741 TTGCTTCATC AAGACTGCAG AGGTTAGACA ACTCCACTGT TCGCACATAC TCATGTACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//