LOCUS       MR211855                8634 bp    DNA     circular  SYN 
DEFINITION  Sec24b (Myc-DDK-tagged) - Mouse Sec24 related gene family, member B (S. cerevisiae) (Sec24b)
ACCESSION   NM_207209
VERSION     NM_207209.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="Sec24b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCCCC GCCGGGTCCC CTCACCCGGC CGCCGGCGCC CGGATGCCGC 
     1081 CCAAGCTCGG CGGAGCTGTC TCCGGCCTGG CACCGCCGCA GCAGAACGGT CCAGCACAGA 
     1141 GTCCAATGCA AGTTCCATCT GGGTATGGGC TGCCTCATCA AAACTATATG GTCCCTTCAG 
     1201 GACATTACTC TCAAGGACCT GGGAAAATGA CCTCCTTGCC ATTGGATAGC CAGTGTGATA 
     1261 GTTACTACTC TCGTCCATAT ACAGTACCAA CACAAAATTC GGGGACTCCC AGCTCAGCAA 
     1321 ACCAACCAGG AGCACAGCAG ATGTATGGCA GAGGTCCTTC CGCTCCTCAC ATGGGGGGCT 
     1381 CCATGCCCGG ATCTTTCCAA GGAATTCCAG CATCAGCATC CCATTCCTAT TCGAGTGCCT 
     1441 CCCAGCCCTA CTCCTCTCTT GGGAATCGCT ACAGTAGTCC TACCACGTAC TCTGCCAATG 
     1501 CTTCTGTTGC TTCTCAGGGA TATCCCTCTA CCTGTAGTCA TTATCCCATC TCAACTGTGT 
     1561 CTAATGTGGT GTATCCTAAT GTTTCCTATC CCTCGCTGCC TGCCAGTGAG CCATATGGGC 
     1621 AGATGTTTAC CTCACAGAGT GCTCCTCCAC CTGCCAGGCC AGTTAAAGAG TCATACTCTG 
     1681 GCCCCAGCAC AGCTCTTACC TACCCCTCGA GACCTCCACC TCCACCTTCT CAGCACCAGC 
     1741 AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GAGTCATTCA GGATATAGTT 
     1801 CTCTACCATG GTCAGGTCCA GCCCTTCCAC CAGCCCAAGA CAGCCTCATC CGAAACCAAA 
     1861 TGGGATCCTT GGCTACAGCG AACAGCCACC CAACTAATAA TGAAAATGTC CAACCTCCCA 
     1921 AGTCAAGCTC CGTAGTATCC ACAGTTTTGC CAGGACCCTC ATCAACAAGA ATGCCACCTG 
     1981 CTCCAAGTCA CCCAGTTGGG CCTGTGCCCT CTGCTCCACC CCCCCCAGAG CAGATGCAGA 
     2041 CTAAAGGCAT GCAGTATGGT GACTATGGTA ATAATCAAGC TAGCTCCACA GCGACTCCCT 
     2101 TGTCATCAGC TTCCGATGAT GAGGAGGAGC AGGAGGAGGA TGAGGAAGCA GGTGTTGACA 
     2161 GCTCTTCCAC CACCAGCAGT GCTTCGCCCT TGCCCAACAG TTACGATGCC TTGGAAGGAG 
     2221 GCAGCTACCC AGATATGCAT TCTTCTTCAG CAAGCAGCCC TGTTCCTGAC CATGCCCTGG 
     2281 AACCCAGTCC CACCCTCGCC CAGGCTCTGT CTGCAGCCCC CACTCCCCCT GCAGCTCAGC 
     2341 CAGCCAAGGT GGCAAAGCCT TTTGGCTATG GCTACCCAGC TCTGCAGCCT GCCTATCAGA 
     2401 ACGCAGCACC ACCACCCATG CCCGCAGCAC ATCCCAGCGG CCCAGCGTAC ACTGGATATC 
     2461 CTCAGCATTA CCCAGGTGTG AACCAGCTGT CCTCAGGTCT CGGAGGACTA AGCCTGCAGA 
     2521 GTTCTCCACA GCCAGAAAGC CTAAGACCTG TCAACCTCAC CCAGGAGAAA AATATTTTAC 
     2581 CTCCAACTCC TATTTGGGCT CCTGTACCTA ACCTGAGTGC AGAACTCAGC AAGTTAAACT 
     2641 GTAGTCCAGA CTCGTTTCGA TGTACCTTGA CCAGTATCCC CCAGACACAG GCTTTACTGA 
     2701 ATAAAGCTAA GCTTCCTTTA GGATTGCTGT TGCATCCCTT CAGAGACCTG ACGCAATTGC 
     2761 CAGTGATAAC CTCAAACACC ATCGTGAGGT GCCGGTCCTG TCGGACGTAC ATCAACCCTT 
     2821 TTGTGTCCTT CATTGATCAG CGAAGATGGA AATGCAACCT GTGTTATAGA GTTAATGATG 
     2881 TTCCAGAAGA GTTTTTGTAC AACCCCCTGA CTCGGTCTTA CGGAGAGCCT CATAAACGGC 
     2941 CAGAAGTCCA GAATTCTACT GTGGAGTTCA TCGCATCCTC AGACTACATG CTCCGGCCTC 
     3001 CTCAGCCCGC GGTCTACTTG TTTGTTTTAG ATGTGTCTCA TAACGCAGTG GAAGCTGGGT 
     3061 ATTTGACAGT TTTATGCCAG TCCTTATTAG AAAACTTAGA CAAGCTTCCT GGAGATTCAC 
     3121 GAACAAGAAT AGGATTCATG ACTTTTGACA GCACCATTCA CTTCTACAAT CTACAAGAAG 
     3181 GGTTATCCCA GCCGCAGATG TTGATTGTAT CTGATATAGA CGATGTTTTT CTGCCCACGC 
     3241 CGGATAGTCT GCTTGTGAAT CTGTATGAAA GCAAAGAGCT TATAAAAGAC TTACTGAATG 
     3301 CATTACCAAG TATGTTCATC AATACCAGAG AAACACACAG TGCCCTTGGA CCTGCACTTC 
     3361 AGGCTGCCTT CAAACTCATG TCTCCCACGG GCGGCCGCGT GTCTGTATTT CAGACACAGT 
     3421 TACCTTCCTT GGGGGCAGGA CTTCTGCAGT CCAGAGAAGA TCCGAATCAG AGGTCGAGTA 
     3481 CCAAGGTGGT GCACCATCTT GGCCCTGCAA CCGACTTTTA TAAGAAGCTC GCTTTAGATT 
     3541 GCTCAGGGCA GCAAACTGCA GTGGATTTGT TCCTTCTAAG TTCACAGTAT TCTGACCTTG 
     3601 CTTCTCTGGC TTGCATGTCC AAGTACTCTG CAGGGTGCAT ATTCTACTAT CCATCTTTCC 
     3661 ACTCTACCCA CAATCCTTCG CAAGCAGAAA AGTTACAAAA AGACCTAAAA CGGTACCTCA 
     3721 CGAGGAAGAT TGGATTTGAA GCTGTTATGA GAATAAGATG CACTAAAGGG CTCTCAATGC 
     3781 ACACTTTCCA CGGGAACTTC TTTGTCCGCT CCACCGACTT ACTGTCTCTT GCCAACATCA 
     3841 ATCCTGATGC TGGGTTTGCA GTACAGTTGT CAATCGAAGA GAGCCTGACA GATACTGCCT 
     3901 TAGTGTGCTT TCAGACAGCC CTGCTGTACA CATCTAGCAA AGGCGAGCGG AGAATTCGAG 
     3961 TGCATACACT GTGCTTGCCA GTCGTAAGTT CCCTCGCTGA CGTTTATGCA GGAGTGGATG 
     4021 TGCAAGCCGC TGTCTGCCTT CTCGCCAACA TGGCTGTGGA CCGCTCAGTG TCATCGAGCC 
     4081 TGTCGGATGC CAGGGATGCC CTGGTGAACG CTGTGGTGGA TCCCCTGTCT GCCTACAGCT 
     4141 CCGCCGTGGC AAGTGTGCCC CGCTCCACCC TGACAGCACC AAGCTCACTT AAGCTGCTCC 
     4201 CACTCTATGT TTTGGCACTT CTTAAACAGA AAGCATTTAG AACAGGGACA AGCACACGCC 
     4261 TGGATGACCG TGTCTATGCC ATGTGCCAGA TGAAGTCGCA GCCGCTTGTG CATCTCATGA 
     4321 AAATGATTCA TCCCAACTTA TACAGGATAG ACAGGCTGAC AGACGAGGGC GCCATCCACG 
     4381 TGAATGACAG GGTAGTACCT CAGCCACCGC TTCAGAAGCT GTCTGCAGAG AAGCTGACCC 
     4441 GAGAAGGCGC TTTCCTCATG GACTGCGGCT CAGTTTTCTA CATTTGGGTT GGCAAAGGCT 
     4501 GTGACAGTAA CTTCATAGAG AACGTGCTTG GATACCCTGA CTTTGCATCA ATACCACAGA 
     4561 AAATGACACA TCTTCCAGAG CTAGACACAC TTCCATCAGA AAGAACTAGA TCCTTTGTGA 
     4621 CTTGGCTCAG GGACAGCCGA CCGTTAAGCC CTGTCCTTCA CTTGGTAAAA GATGAGAGCC 
     4681 CTGCCAAAAC AGACTTTTTC CAGCATTTGC TTGAAGACCG CACGGAAGCC GCACTCTCCT 
     4741 ACTATGAGTT TCTGATTCAC ATCCAACAGC AGGTTTGTAA GACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//