LOCUS       MR211854                8628 bp    DNA     circular  SYN 
DEFINITION  Tpp2 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147)
ACCESSION   BC058239
VERSION     BC058239.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="Tpp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCGCC GCGACCGAGG AGCCCTTTCC TTTCCACGGT CTTCTACCAA 
     1081 AGAAGGAGAC CGGGGCCTCC TCCTTCCTGT GCCGCTACCC GGAGTATGAC GGGCGCGGGG 
     1141 TGCTCATCGC CGTCCTGGAC ACAGGGGTCG ATCCCGGGGC CCCGGGCATG CAGGTTACAA 
     1201 CTGATGGAAA ACCAAAAATC ATTGATATCA TTGATACAAC AGGAAGTGGT GATGTAAATA 
     1261 CTGCTACAGA AGTAGAACCA AAAGATGGTG AAATTATTGG TCTTTCTGGA AGAGTGCTTA 
     1321 AGATTCCTGC AAACTGGACA AATCCTTTAG GAAAATATCA TATTGGCATT AAAAATGGTT 
     1381 ATGACTTCTA TCCAAAGGCT CTCAAGGAAA GGATACAGAA AGAACGGAAG GAAAAAATCT 
     1441 GGGATCCAAT TCACAGAGTT GCACTTGCAG AAGCTTGTAG AAAACAAGAA GAATTTGATA 
     1501 TTGCCAACAA TGGCTCTTCC CAAGCCAATA AACTAATCAA GGAAGAGTTG CAAAGTCAAG 
     1561 TGGAATTACT TAATTCTTTT GAGAAAAAGT ATAGTGATCC AGGCCCTGTA TATGACTGCT 
     1621 TGGTGTGGCA TGATGGTGAG ACCTGGAGAG CCTGTGTTGA TTCAAATGAG AATGGGGACT 
     1681 TGAGTAAATG TGCCGTATTG AGAAACTACA AAGAGGCCCA AGAGTACAGT TCTTTTGGCA 
     1741 CAGCTGAGAT GCTGAATTAC TCTGTGAACA TTTATGACGA TGGGAACCTG CTCTCCATTG 
     1801 TGACCAGCGG AGGAGCTCAT GGAACCCATG TAGCAAGTAT AGCCGCAGGG CATTTTCCAG 
     1861 AAGAGCCTGA ACGGAATGGA GTTGCTCCTG GTGCTCAAAT TCTATCCATT AAGATTGGTG 
     1921 ATACACGGCT AAGCACTATG GAAACAGGCA CAGGCCTCAT CAGAGCTATG ATAGAAGTTA 
     1981 TAAATCATAA GTGTGATCTT GTCAACTACA GTTATGGAGA AGCAACTCAT TGGCCAAATT 
     2041 CTGGGAGAAT TTGTGAAGTA ATTAATGAAG CAGTATGGAA ACATAATACA ATTTATGTTT 
     2101 CAAGTGCTGG AAATAATGGT CCATGCCTTT CTACAGTGGG TTGTCCAGGA GGAACTACAT 
     2161 CCAGTGTGAT AGGTGTTGGA GCTTATGTTT CCCCTGATAT GATGGTTGCA GAGTATTCAC 
     2221 TGAGAGAGAA ACTGCCTGCA AATCAATATA CATGGTCTTC TAGAGGCCCA AGTGCTGATG 
     2281 GAGCCCTCGG TGTGAGCATC AGTGCACCAG GAGGTGCTAT TGCTTCTGTG CCTAACTGGA 
     2341 CATTGAGGGG GACTCAGCTA ATGAATGGGA CATCAATGTC TTCCCCCAAT GCCTGTGGTG 
     2401 GCATTGCCCT GGTACTTTCA GGGCTGAAAG CAAATAATGT TGACTATACT GTACACTCAG 
     2461 TCAGAAGAGC TCTAGAAAAT ACTGCAATAA AAGCTGACAA TATAGAAGTA TTTGCCCAAG 
     2521 GACATGGAAT TATTCAGGTT GACAAAGCTT ATGACTACCT CATTCAAAAT ACATCATTTG 
     2581 CTAACAGATT AGGTTTTACA GTTACTGTTG GAAATAACCG TGGTATCTAC CTCCGAGATC 
     2641 CTGTCCAGGT GGCTGCTCCT TCAGATCATG GTGTTGGCAT TGAGCCTGTA TTTCCAGAGA 
     2701 ACACAGAAAA CTCTGAAAAA ATATCCTTTC AGCTTCATTT AGCTTTAACT TCAAATTCAT 
     2761 CTTGGGTTCA GTGTCCCAGC CATTTGGAAC TCATGAATCA GTGTCGGCAC ATAAACATAC 
     2821 GTGTGGACCC CAGGGGCTTA AGAGAAGGGT TACATTATAC AGAGGTATGT GGCTATGATA 
     2881 TAGCATCCCC CAATGCAGGT CCTTTATTCA GAGTTCCAAT CACTGCAGTT ATAGCAGCAA 
     2941 AAGTAAATGA GTCATCACAT TATGATCTAG CCTTTACAGA TGTACATTTT AAACCTGGTC 
     3001 AGATTCGAAG ACATTTTGTT GAGGTTCCTG AAGGGGCAAC CTGGGCTGAA GTTACCGTGT 
     3061 GTTCATGTTC TTCTGAGGTA TCGGCAAAAT TTGTTCTTCA TGCAGTACAG CTTGTGAAGC 
     3121 AGAGAGCATA TCGAAGTCAT GAATTTTATA AGTTTTGTTC CCTTCCAGAA AAAGGAACAC 
     3181 TTATTGAAGC TTTTCCTGTT TTGGGCGGAA AAGCAATTGA ATTTTGTATT GCTCGTTGGT 
     3241 GGGCAAGTCT TAGTGATGTC AATATTGATT ATACCATATC ATTCCATGGG ATAGTGTGTA 
     3301 CTGCACCACA GTTAAACATT CATGCATCTG AAGGAATCAA TCGTTTTGAT GTTCAGTCCT 
     3361 CTTTAAAGTA TGAAGATCTG GCTCCTTGCA TAACTTTGAA GAGCTGGGTG CAAACGCTAC 
     3421 GCCCAGTAAA TGCAAAAACC AGACCTTTAG GATCAAGAGA TGTTTTGCCA AATAATCGCC 
     3481 AGCTTTATGA GATGGTCCTT ACATACAGCT TTCATCAGCC CAAGAGCGGA GAAGTAACAC 
     3541 CTAGTTGTCC ACTCCTTTGT GAATTGTTAT ATGAGTCAGA ATTTGACAGT CAGTTGTGGA 
     3601 TTATTTTCGA CCAGAACAAA AGACAGATGG GCTCAGGCGA TGCCTATCCA CATCAGTATT 
     3661 CTCTGAAATT GGAGAAAGGA GATTATACAA TTCGATTACA GATTCGTCAT GAGCAAATCA 
     3721 GTGATTTGGA TCGTCTCAAA GATCTTCCGT TTATTGTTTC ACATAGGTTG TCTAATACCT 
     3781 TGAGCTTAGA TATTCATGAA AATCATAGCC TTGCACTTCT AGGAAAGAAG AAATCAAGCA 
     3841 GCTTAACATT ACCACCCAAA TACAATCAGC CATTCTTTGT TACTTCCTTA CCTGATGATA 
     3901 AAATACCTAA GGGGGCAGGA CCCGGATGCT ACCTTGCAGG CTCCTTGACA TTGTCAAAGA 
     3961 CTGAGCTTGG AAAGAAAGCT GATGTGATCC CTGTTCATTA CTATCTCATA CCTCCACCAA 
     4021 CAAAGATTAA GAATGGCAGC AAAGATAAAG AAAAGGATTC AGAAAAAGAG AAAGACTTGA 
     4081 AAGAAGAGTT TACTGAAGCA TTACGCGATC TCAAAATTCA GTGGATGACC AAGCTTGATT 
     4141 CTACTGACAT TTACAATGAA TTGAAAGAAA CATATCCTGC TTACCTTCCT TTGTATGTTG 
     4201 CACGTCTTCA TCAACTGGAT GCTGAAAAGG AACGAATGAA AAGACTTAAT GAAATTGTTG 
     4261 ATGCTGCCAA TGCTGTTATT TCTCACATCG ATCAAACCGC TCTTGCAGTT TACATTGCAA 
     4321 TGAAGACTGA CCCCAGGCCT GATGCAGCTA CTATAAAAAA TGATATGGAC AAGCAGAAAT 
     4381 CCACCCTGAT AGATGCCCTC TGCAGGAAAG GATGTGCCCT GGCAGATCAC CTTCTTCATA 
     4441 CACAGCCTCA CGACGGGGCA GCAGCTGGAG ATGCTGAAGC AAAGGAAGAA GAAGGAGAAA 
     4501 GTACCATGGA ATCTCTATCG GAAACCTATT GGGAAACTAC AAAGTGGACA GATCTTTTTG 
     4561 ACACTAAGGT TTTGATATTT GCATACAAGC ATGCATTAGT AAATAAGATG TATGGGAGAG 
     4621 GCCTTAAGTT TGCAACCAAA CTCGTAGAAG AAAAACCAAC AAAAGAAAAC TGGAAAAATT 
     4681 GTATTCAACT CATGAAATTA CTTGGATGGA CCCACTGTGC ATCTTTTACT GAAAACTGGC 
     4741 TCCCCATCAT GTATCCTCCT GATTATTGTG TATTCACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//