LOCUS       MR211853                8628 bp    DNA     circular  SYN 
DEFINITION  Rev1 (Myc-DDK-tagged) - Mouse REV1 homolog (S. cerevisiae) (Rev1)
ACCESSION   NM_019570
VERSION     NM_019570.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="Rev1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCGAGGT GGATGGCGGA AGAGAACGGA GAATGATGGC TGGGAAAAAT 
     1081 GGGGCGGGTA CATGGCTGCC AAGGTCCAGA AGCTGGAGGA GCAGTTCCGC ACAGATGCTG 
     1141 CTAACCAGAA GGATGGGACG GCGTCTGCAA TATTTAGTGG AGTTGCCATC TATGTTAATG 
     1201 GATATACAGA TCCTTCTGCG GAGGAATTGA GAAATCTAAT GATGTTGCAT GGAGGTCAAT 
     1261 ACCATGTGTA TTATTCTAGA TCCAAAACAA CACACATTAT TGCTACAAAT CTTCCTAATG 
     1321 CCAAAATTAA AGAATTAAAG GGGGAAAAAG TAATACGACC AGAATGGATT GTGGAAAGCA 
     1381 TCAAAGCTGG GAGACTCTTG TCCTCTGCTC CCTATCAGCT CTACACCAAG CCGTCGGCTG 
     1441 CACAGAAGAG TCTCAACTTC AATCCTGTGT GCAAACCTGA GGATCCTGGG CCAGGTCCAA 
     1501 GCAATAGAGC CAAACAGCTC AACAACAGGG TAAATCACAT AATTAAGAAG ATTGAGACAG 
     1561 AAAGTGAAGT GAAAGCAAAT GGACTGAGCA GCTGGAATGA AGACGGTGTA AATGATGATT 
     1621 TTAGTTTTGA GGATCTGGAA CATACATTTC CAGGAAGGAA ACAGAATGGA GTAATGCATC 
     1681 CCAGAGATAC CGCTGTCATT TTTAATGGAC ACACCCATAG TTCAAATGGT GCCTTAAAGA 
     1741 CACAGGATTG CTTGGTGCCT GTGGGCAACA GTGTTGCTAG CAGGCTATCT TTAGACTCCA 
     1801 CCCAGGAGGA GAAGAGGGCA GAGAAGAGCA ACGCGGACTT CAGAGACTGC ACAGTGCAGC 
     1861 ACTTGCAACA CAGCACCAGA AGTGCAGATG CTTTGCGGAG TCCACACAGA ACTAATTCTC 
     1921 TCTCACCATC TTTGCACAGT AACACTAAAA TCAATGGTGC TCACCACTCC ACTGTTCAGG 
     1981 GGCCTTCAAG CACAAAAAGC ACTTCTGTTC TGACTCTTAG TAAAGTAGCG CCTTCAGTGC 
     2041 CATCCAAACC CTCGGACTGC AACTTCATTT CAGATTTCTA TTCTCGTTCA AGACTGCATC 
     2101 ACATCTCAAC ATGGAAGTGT GAATTGACTG AGTTTGTCAA TACCCTACAG AGACAAAGTA 
     2161 GTGGTATCTT TCCGGGAAGG GAAAAGTTAA AAAAAGTGAA AACAGGCAGG TCTTCTCTTG 
     2221 TTGTAACTGA CACAGGCACT ATGTCAGTGT TGAGCTCACC CAGACATCAG AGCTGTGTGA 
     2281 TGCACGTTGA TATGGACTGC TTCTTTGTAT CAGTGGGTAT ACGAAATAGA CCAGATCTCA 
     2341 AAGGAAAACC AGTGGCTGTT ACAAGTAACA GAGGCACGGG AACGGCGCCT TTGCGTCCTG 
     2401 GTGCTAACCC GCAGCTGGAA TGGCAGTATT ACCAGAATAG AGCCCTGAGA GGAAAAGCAG 
     2461 CAGATATACC AGATTCATCA GTATGGGAGA ATCAAGATTC TACACAAACA AATGGAATTG 
     2521 ATTCTGTTCT GTCAAAAGCT GAGATTGCTT CTTGTAGTTA TGAAGCCAGA CAAGTTGGCA 
     2581 TTAAGAACGG AATGTTTTTT GGATATGCTA AACAGCTGTG TCCTAATCTG CAAGCTGTTC 
     2641 CGTATGATTT TCATGCATGC AGGGAAGTTG CCCAGGCAAT GTATGAAACA CTGGCCAGCT 
     2701 ACACACACAG CATCGAGGCT GTCAGCTGCG ATGAAGCACT GATTGACGTC ACGGACATCC 
     2761 TTGCAGAGAC GAAACTTTCT CCTGAGGAGT TTGCAGCTGC CCTCCGTATT GAAATCAAAG 
     2821 ACAAGACCAA ATGTGCTGCC TCTGTTGGAA TTGGTTCTAA TATTCTTCTT GCTAGAATGG 
     2881 CAACTAAGAA AGCAAAGCCA GATGGTCAGT ACCACCTGCA GCCCGACGAG GTAGACGATT 
     2941 TCATCCGAGG GCAGCTCGTC ACTAACCTGC CGGGAGTTGG ACGTTCAATG GAATCTAAGC 
     3001 TTGCATCTTT GGGGATTAAG ACTTGTGGAG ATTTGCAGTG TTTGACCATG GCAAAACTCC 
     3061 AGAAAGAATT TGGCCCCAAG ACAGGGCAGA TGCTGTATAG GTTCTGCCGA GGTTTGGATG 
     3121 ATAGACCTGT TAGAACAGAG AAGGAACGAA AATCTGTTTC AGCCGAGATC AACTATGGAA 
     3181 TAAGGTTCAC CCAGCCCAAA GAAGCAGAAG CTTTCCTTCT GAGTCTTTCA GAAGAAATTC 
     3241 AAAGACGACT TGAAGCTGCT GGCATGAAGG GCAAACGCCT GACTCTGAAA ATCATGGTGC 
     3301 GGAAGCCAGG GGCCCCTATA GAAACTGCAA AATTTGGAGG CCATGGAATT TGTGATAACA 
     3361 TCGCCAGGAC TGTAACTCTG GACCAGGCAA CAGATAGTGC AAAAATAATT GGGAAAGCCA 
     3421 CTCTAAACAT GTTTCATACA ATGAAACTAA ATATATCGGA TATGAGAGGG GTTGGTATTC 
     3481 AAGTGAATCA GTTGGTTCCT GCTAATTCAA ATCTTTCCAC ATGTTCAAGT CGTCCATCAG 
     3541 CTCAGTCAAG CCTCTTCTCT GGTCGGCCAC ATTCTGTCCG TGATCTCTTC CAACTTCAGA 
     3601 AAGCCAAGAA ACCCACAGAA GAGGAACACA AGGAAGTATT TCTGGCTGCT GTGGATCTGG 
     3661 AAGTGTCCTC CACCTCTAGA GCTTGTGGTT TATTGTCTCC GCTCTCTGCA CATCTGGCAG 
     3721 CTAGTGTTAG TCCTGACACT AACAGTGGAG AGTGTTCCAG AAAATGGAAT GGCCTGCACT 
     3781 CTCCTGTCAG TGGACAGTCA CGGCTGAACC TCAGTATAGA GGTCCCATCA CCATCCCAGA 
     3841 TTGACCAGTC TGTTTTAGAA GCGCTCCCAC TTGATCTCCG GGAACAAATA GAACAAGTTT 
     3901 GTGCCGCTCA GCAGGGAGAG CCACGTGGAA AGAAGAAAGA ACCAGTAAAT GGCTGCAGTT 
     3961 CAGGGGTGTT GCCACATCCT GTTGGAACAG TTTTATTACA GATCCCAGAG CCTCAAGAAC 
     4021 CTTGTAATAG TGACAGCAAG ATTAGTGTAA TTGCCCTTCC AGCATTCTCA CAAGTGGACC 
     4081 CTGATGTCTT TGCTGCCCTT CCTGCTGAGC TTCAAAAGGA GCTGAAAGCA GCATATGATC 
     4141 AAAGACAAAG GCAGGGAGAG GACACTACTC ATCAGCAGCC AACCAGTACA TCTGTGCCAA 
     4201 AGAATCCTTT ACTTCAGCTA AAGCCACCAG CAATGAAAGA CAAGAGGAAC AAGAGGAAGA 
     4261 ACCTCATTGG TTCTCCCAGA AAGAGTCCTT TGAAAAATAA GCTGCTTAGC AGTCCTGCAA 
     4321 AAACTCTGCC AGGGGCATAT GGAAGCCCCC AGAAGTTAAT GGATGGTTTT CTACAACATG 
     4381 AAGGAATGGC TTCAGAGAGA CCCCTGGAAG AAGTCTCGGC TTCTACCCCT GGTGCACAGG 
     4441 ACCTATCTAG TTTACTGCCA GGCCAGAGCA GCTGTTTTAG ACCAGCAGCA CCCAACCTAG 
     4501 CTGGAGCTGT GGAATTCAGT GATGTGAAGA CCTTGCTCAA AGAGTGGATC ACTACTATCT 
     4561 CAGATCCGAT GGAAGAAGAC ATCCTGCAGG TTGTGAGATA CTGCACTGAC CTCATAGAGG 
     4621 AGAAAGACTT GGAAAAATTG GATCTGGTTA TAAAATACAT GAAAAGGTTG ATGCAGCAGT 
     4681 CGGTGGAGTC TGTATGGAAC ATGGCGTTTG ACTTTATTCT TGACAATGTT CAGGTGGTTT 
     4741 TACAGCAGAC TTATGGAAGC ACACTGAAAG TGACCACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//