LOCUS       MR211848                8613 bp    DNA     circular  SYN 
DEFINITION  Skiv2l (Myc-DDK-tagged) - Mouse superkiller viralicidic activity 2-like (S. cerevisiae) (Skiv2l)
ACCESSION   NM_021337
VERSION     NM_021337.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4770..4861 
                     /label="Myc-DDK"  
     misc_feature    4891..5476 
                     /label="PolyA signal"  
     misc_feature    4948..4967 
                     /label="Restriction Enzyme"  
     misc_feature    5752..6424 
                     /label="ColE1"  
     misc_feature    6854..7648 
                     /label="Kan/Neo"  
     misc_feature    7683..7985 
                     /label="SV40 ori"  
     misc_feature    8071..8548 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4752..4758 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4752 
                     /label="Skiv2l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGAGACG GAGCGACTCG TTCTGCCTCC CCTAGATCCC CTGAACTTAC 
     1081 CCCTTCGAGC TCTGGAAGTG GGGTGCACTG GCCGTTGGGA GCTGCTGAAC GTGCCAGGGC 
     1141 CTCCAGAGAG CACTCTCCCC CATGGCCTCC CTCCATGCGC CCCAGATCTG TGCCAGGAAG 
     1201 CAGAGCAGCT GTTTCTGTCG TCTCCAGCCT GGCTTCCTCT ACACGGTGTA GAGCACTCAG 
     1261 CTCGAAAATG GCAGAGGAAG ACGGATCCCT GGTCACTCCT GGCTGCCGTG GAGACTCCAG 
     1321 TCCCATCTGA CCTTCAGGCC CAGAGACACC CAACCACAGG CCATATACTG GGCTATAAGG 
     1381 AGGTCCTGCT GGAGAACACA AATCTGTCAG CTACAACCTC CTTGTCCCTT CGCCGGCCTC 
     1441 CAGGGCCAGC CTCCCAGTCG CTATGGGGCA ATCCAACACA GTACCCTTTC TGGCCAGGTG 
     1501 GGATGGATGA GCCCAGCATA ACTGATCTGC ATACTCGGGA GGAAGCTGAG GAGGAGATAG 
     1561 ACTTTGAGAA AGATCTTCTC ACTGTCCCAC CTGGCTTCAA GAAAGGGGTG GATTTTGCCC 
     1621 CCAAAGATCA TCCAGCTCCT GTGCCAGGTT TGCTCAGCCT CAGCCGTCTG CTGGAGCCTC 
     1681 TTGATCTGAG TGGAGGTGAC GAGGATGAAG GCGAGGCAGC AGGAGGCCCT AGAGGGGACA 
     1741 ATGCCTCCCC CTCCCCCTCT GGTACTCCCC TGGTCCGAGC AAGCAGCTTG GAGGATCTAG 
     1801 TGTTGAAGGA AGCTGCCACA GTCGTATCTA CCCCGGAGCC TCCCAAACCT CCACCTCAGG 
     1861 AACAGTGGGC TGTGCCTGTG GACGTCACCT CCCCTGTGGG CGACTTTTAC CGTCTCATCC 
     1921 CTCAGCCAGC TTTCCAGTGG GCATTTGAGC CAGATGTGTT TCAGAAGCAG GCCATCCTGC 
     1981 ACTTGGAGCA GCACGACTCT GTCTTTGTTG CAGCTCACAC ATCTGCTGGG AAGACAGTGG 
     2041 TGGCTGAATA TGCCATCGCC CTGGCCCAGA AACACATGAC TCGTACCATC TACACTTCTC 
     2101 CTATCAAAGC CCTGAGCAAC CAGAAGTTTC GGGACTTCCG GAACACATTT GGGGATGTAG 
     2161 GGCTGCTCAC AGGGGATGTG CAGCTGCACC CAGAGGCCTC CTGCCTCATC ATGACCACGG 
     2221 AGATCCTTCG CTCCATGCTG TACAGTGGCT CCGACGTCAT CCGAGACCTG GAGTGGGTCA 
     2281 TCTTTGATGA AGTCCACTAT ATCAATGATG CTGAGCGTGG GGTCGTGTGG GAGGAGGTGC 
     2341 TCATCATGCT TCCTGAGCAC GTCTCCATCA TCCTTCTGAG TGCCACCGTC CCCAATGCCC 
     2401 TGGAGTTTGC TGACTGGATC GGGCGGCTGA AGCGGAGGCA GATCTATGTG ATCAGCACGG 
     2461 TTGCCCGGCC GGTCCCCCTG GAGCACTATC TCTTCACAGG GAACAGCCCC AAGACGCAGG 
     2521 GCGAGCTCTT CCTGCTGCTG GACTCCCGAG GGGCCTTCCA CACCCAGGGG TACTATGCTG 
     2581 CAGTGGAGGC CAAGAAAGAG AGGATGAGCA AGCACGCCCA GACTTTCGGG GCCAAGCAGC 
     2641 CCACGCACCA GGGTGGGCCT GCACAGGACC GCGGGGTGTA CCTGTCCCTG CTGGCTTCCC 
     2701 TCCGGACCCG GGCTCAGTTG CCAGTGGTAG TGTTCACCTT CTCCCGAGGC CGCTGTGATG 
     2761 AGCAGGCTTC GGGCCTCACC TCTCTAGACC TCACGACAAG CTCGGAGAAG AGTGAGATCC 
     2821 ACCTCTTCCT GCAGCGCTGC CTTGCACGGC TCCGAGGCTC TGACCGACAG CTACCCCAGG 
     2881 TCCTGCACAT GTCGGAGCTC CTTCGCCGAG GCCTAGGTGT GCACCACAGC GGCATCCTGC 
     2941 CCATCCTTAA GGAGATTGTG GAAATGCTTT TCAGCAGGGG CCTGGTCAAG GTCTTGTTTG 
     3001 CCACCGAGAC TTTTGCCATG GGGGTAAACA TGCCAGCCAG GACGGTGGTG TTTGACTCCA 
     3061 TGCGCAAACA TGATGGCTCT ACCTTCCGGG ACCTGCTGCC TGGGGAGTAC GTGCAGATGG 
     3121 CAGGCCGGGC AGGCCGGAGG GGCCTGGACC CCACGGGCAC CGTCATCCTT CTCTGTAAGG 
     3181 GCCGAGTGCC TGAGATGGCA GATCTACACC GCATGATGAT GGGGAAACCA TCCCAACTTC 
     3241 AGTCCCAGTT CCGCCTCACA TATACCATGA TCCTCAACCT GCTGCGGGTG GATGCCCTCA 
     3301 GAGTGGAGGA CATGATGAAG AGGAGCTTCT CAGAGTTCCC ATCCCGCAAG GACAGCAAGG 
     3361 CCCATGAGCA GGCTCTGGCT GACCTCACCA AGAGGCTGGG GGCTTTGGAG GAGCCTGATG 
     3421 TGACTGGTCA GTTGGCTGAC CTACCTGAGT ATTACAGCTG GGCGGAGGAG CTGACAGAGA 
     3481 CCCAGAACAT GATCCAGCGA CGCATCATGG AGTCTGTGAA TGGGCTGAAA TCTCTCTCAG 
     3541 TGGGAAGGGT GGTAGTTGTG AAGAATGAGG AACATCACAA TGCACTGGGT GTGATCCTGC 
     3601 AGGTCTCCTC AAACTCCACC AGCAGGGTAT TTACAACATT GGTCTTGTGT GATAAGCCTG 
     3661 TTGTGTCTGA TAACCCAAGG GACAAGGGGC CAGCCACTCC AGATGTGCCC CACCCAGATG 
     3721 ACCTTATAGG CTTCAAGCTG TTCCTGCCTG AAGGGCCCTG TGAGCACACC GTGGCCAAGC 
     3781 TCCAGCCAGG AGATGTGGCT GCCATCTCTA CCAAAGTGCT CCGGGTGAAT GGGGAGAAGA 
     3841 TCTCTGAGGA CTTTAGCAAG AGGCAGCAAC CAAAATTCAG AAAGGACCCT CCCCTTGCGG 
     3901 CTGTGACCAC TGCTGTTCAG GAGCTGCTGC GTCTGGCTCA GTCCTATCCA GCAGGACCCC 
     3961 CAACCCTTGA CCCTATCAAC GACCTGCAAC TCAAGGATGT GGCAGTGGTA GAAGGTGGGC 
     4021 TCCGGGCCCG GAAGCTGGAG GAGCTGATCC GCGGGGCTCA GTGTGTGCAC AGCCCCCGGT 
     4081 TTCCTGCCCA GTACGTGAAG CTGCGGGAGC GAATGCAGAT TCAGAAGGAA ATGGAGAGGC 
     4141 TGCGCTTCCT CCTGTCTGAC CAGTCGCTGT TACTGCTCCC TGAGTACCAC CAGCGTGTAG 
     4201 AGGTGCTCCG GACTCTGGGC TATGTGGATG AGGCTGGCAC AGTGAAGCTG GCAGGCCGAG 
     4261 TGGCCTGTGC CATGAGCAGC CATGAGCTGC TCCTCACTGA ACTCATGTTT GACAATGCAC 
     4321 TGAGCGCTCT GCGACCAGAG GAAATCGCAG CTCTGCTCTC TGGCCTGGTG TGCCAGAGCC 
     4381 CCGGGGACCC TGGCGACCAG CTCCCAAGTA CCCTCAAACA GGGGGTAGAA CGAGTCAAAG 
     4441 CTGTGGCCAA GCGGATTGGC GAGGTTCAGG TGGCCTGTGG CCTGAACCAG ACAGTGGAGG 
     4501 AGTTTGTGGG AGAGCTGAAT TTTGGGCTGG TAGAGGTTGT GTATGAGTGG GCTAGGGGTA 
     4561 TGCCCTTCTC CGAGTTGGCA GGGCTCTCAG GGACCCCTGA GGGCTTGGTC GTCCGCTGCA 
     4621 TCCAGCGTCT GGCTGAGATG TGTCGCTCGC TTCGAGGAGC AGCACGTTTG GTGGGTGAGC 
     4681 CTGTGCTGGG TGCCAAGATG GAGACGGCAG CCACCTTGTT ACGGCGGGAC ATCGTCTTCG 
     4741 CAGCCAGCCT CTACACCCAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4801 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4861 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4921 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4981 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5041 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5101 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5161 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5221 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5281 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5341 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5401 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5461 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5521 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5581 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5641 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5701 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5761 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5821 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5881 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5941 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6001 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6061 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6121 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6181 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6241 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6301 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6361 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6421 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6481 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6541 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6601 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6661 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6721 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6781 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6841 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6901 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6961 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7021 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7081 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7141 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7201 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7261 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7321 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7381 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7441 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7501 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7561 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7621 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7681 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7741 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7801 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7861 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8041 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8101 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8161 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8221 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8281 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8341 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8401 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8461 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8521 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8581 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//