LOCUS       MR211847                8622 bp    DNA     circular  SYN 
DEFINITION  Pex1 (Myc-DDK-tagged) - Mouse peroxisomal biogenesis factor 1 (Pex1)
ACCESSION   NM_027777
VERSION     NM_027777.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4761 
                     /label="Pex1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG TGGAGCAGCG ATCGCTTAGC GGGTGCGGGG TCTGGTGGGG 
     1081 CGGTCGTGAC GGTGGCCTTC ACGAATGCTC GGGACTGCTT CCTGCATCTG CCGCGGCGCC 
     1141 TCGTGGCCCA GCTTCACCTG CTGCAGAATC AAGCTATAGA AGTGGCCAGC GATCACCAGC 
     1201 CTACCTACCT GAGCTGGGTG GAAGGCAGGC ATTTTAATGA TCAAAGTGAA AATGTGGCAG 
     1261 AAATTAATAG ACAAGTTGGC CAGAAACTTG GACTCTCAAG CGGAGATCAG GTGTTTCTCA 
     1321 GGCCTTGTTC CCATGTGGTA TCTTGCCAAC AGGTTGAAGT GGAGCCTCTC TCAGCAGATG 
     1381 ACTGGGAAAT ACTGGAGCTG CACGCCATTT CCCTTGAACA GCATCTTCTG GATCAGATTC 
     1441 GAATAGTTTT CCCCAAAGCT GTTGTTCCCA TCTGGGTTGA CCAGCAGACC TACATATTTA 
     1501 TCCAGATCGT CACTCTGATG CCAGCTGCCC CTTATGGAAG GCTAGAAACT AACACCAAAC 
     1561 TCCTTATTCA GCCAAAGACA CGCCAAGCCA AAGAGAGCAC ATTTCCAAAA GAAGGAGATG 
     1621 CACACGGACA AGTTCATAGT TATGGGCGAG AACAGAAAGG ATTGTCAAAG GAATTACAAA 
     1681 CCAGGCAGCT TCATACGAAC TCAGAGGGTA TCACTGCATC CAATGGAAGA GACCCAAAAG 
     1741 TCCCAGGTGG CCCGTTGAAG CCAAGCTGGT GGGCTGTGCT CGGAAGCATG CTTTCCTTTG 
     1801 GGCCTGACAG TAAACAGGAG TCAGCCTGGG GCTCGTTGGA ACTCGGTGCT TTCAAAAACA 
     1861 TGCAGTCACA GGCTGCCCCT CTGGAAGGTA CTTTCAGAGT ATGCCAAGTC CAGCCACCTA 
     1921 GTGCGCGTAC CACCACCGCT ACCTCTGTGT TTCACAAACA CTGCACCGCC CACGTATTTC 
     1981 CTTGGGACCA AGAATACTTT GATGTGGAGC CCAGCTTTAC TGTGACCTAT GGAAAACTAG 
     2041 TTAAGCTACA TTCTCCAAAA CAACAGCAAG ACAAAAGTAA GCAGGGTGTC CTGTTGCCTG 
     2101 ACAAAGAGAA GCAGCTGTCC AAGTCTCCAG ATCATAAGCA GATCAGCTCC AACCGCAGTG 
     2161 AGGAGGCTGC TGAGGCCTGT GTGCTGAAGG TAGTCTGGAA TGGACTCGAG GAGTTGAAGA 
     2221 ATGCCACAGA GTTCACCGAA AGTCTAGAGC TTCTCCACCG TGGGAAAGTC TGGCCTAAAG 
     2281 ACGTCAATGA AGAAACTATA AAAACAGTGT TCTCATCTTG GGTACAGCAG TCCGCTACCA 
     2341 CGATGCTTCC TTTGGTAATA TCAAAGGAAG AGCGTATTAA GCTGGAAATT AAAGATGGGC 
     2401 TGAGAGAGTT CTCTCTGAGT ACAGTTCATT CTCAGGAAAA AGAAAAGGAA GAAGGAAAAA 
     2461 CTGTGTTTGT GTTGAGTTCC ATCCTGCTGC AGAAGATCTC AGTACAAGTC CTTCTAGAGC 
     2521 CCATGATAAA AGAAGAACAG AGTGCGGAAA TCGACTTTCT TCTTCCCTCT TTAACGCTGA 
     2581 GCTCTTTGGG GGGAGTGAGT GCCTTAGGTG CATCTGCAAT GGAGCACATC ACTCACAGTC 
     2641 TCCTGGGACG CCCGTTGTCT AGGCAGCTGA TGGCCCTCGT TGCAGGACTT AGGAATGGCG 
     2701 CTCTTTTGAT CACTGGAGGA AAGGGAAGCG GGAAGTCAAC ATTCGCGAAA GCCATCTGCA 
     2761 AGGAAGCACA GGACACTCTG GATGCCCGTG TGGAGACAGT CGACTGCAAG GCTTTACGAG 
     2821 GAAAAAGGCT TGAAAGCATA CAAAAAGCGC TAGAGGTGGC TTTCTCAGAG GCCGCCTGGA 
     2881 GGCAGCCATC TGTCATTCTG CTGGATGACC TGGACCTCAT TGCCGGACTG CCAAGTGTCC 
     2941 CCGAGCAGGA GCACAGCCCT GAAGCGGTGC AGAGCCAGCG GCTTGCACAT GCTTTGAACG 
     3001 ATATGATCAA AGAGTTTGTT TCCACGGGAA GCTTGGTGGC ACTCATCGCC ACGAGCCAGC 
     3061 TCCAGCAGTC TCTGCACCCT TCCCTTGTGT CTGCTCAAGG AATCCACACG TTTCAATGTG 
     3121 TCCAGCACCT TCAGCCTCCC AATCCGGAAC AGAGATGTGA AATTCTGCAC AGTGTTGTGA 
     3181 AGAATAAACT GGGCTGTGAT ATAAGCAATT TCCCTGACTT GGACCTGCAG TGCATAGCTA 
     3241 AAGACACAGA AGCGTTTGTG GCTCGTGACT TTACAGTTCT TGTGGACCGA GCCATACACT 
     3301 CTTCTCTCTC TCGCCAGCAT AGCTCCTCTA GGGAAGACTT GACTTTAACA ACATCAGACT 
     3361 TCCAAAAGGC TCTCCGTGGA TTCCTTCCTG CTTCTCTGCG AAATGTCAAC TTGCATAAAC 
     3421 CTAGAGACCT GGGCTGGGAC AAGATTGGTG GATTACATGA AGTTCGGCAG ATCCTCATGG 
     3481 ATACTATCCA GTTACCAGCC AAGTACCCAG AATTATTTGC AAACTTACCC ATACGACAGA 
     3541 GGACAGGAAT ACTGCTTTAT GGTCCTCCAG GGACAGGAAA AACTTTACTT GCTGGGGTAG 
     3601 TTGCAAGAGA GAGTGGAATG AATTTTATTA GTATTAAGGG ACCAGAGTTA CTCAGCAAAT 
     3661 ATATTGGCGC AAGTGAGCAA GCTGTTCGAG ATGTTTTCAT CAGAGCACAG GCTGCAAAGC 
     3721 CCTGCATTCT TTTCTTTGAT GAGTTTGAGT CCATCGCTCC TCGAAGAGGC CATGACAACA 
     3781 CAGGGGTTAC AGACCGAGTA GTCAACCAGT TGCTGACACA GTTAGACGGA GTAGAAGGCT 
     3841 TACAGGGAGT TTATGTGCTG GCTGCTACTA GTCGCCCTGA CTTGATCGAC CCTGCCCTGT 
     3901 TGCGGCCTGG CAGACTGGAT AAATGTGTAT ACTGCCCTCC TCCAGATCAG GTGTCCCGTC 
     3961 TTGAGATTTT AACTGTCCTC AGCAAGTCTC TAGCTCTGGC AGATGACGTG GACCTTCAGC 
     4021 ACGTGGCGTC GGTCACCGAC TCGTTCACTG GAGCGGATCT GAAAGCTCTG CTGTACAACG 
     4081 CTCAGCTGGA GGCCTTGCAG GGACGGCTGC TGCCCAGTGG GCTTCCCGAT GGAGGCTCCA 
     4141 GCTCTGACAG TGACCTGAGT CTGTCTTCAA TGGTCTTTCT TAACCACAGC AGTGGTTCCG 
     4201 ACGACTCCGC TGGAGATGGA GAATGTGGCT TAGAGCAATC CCTGCTTTCT CTCGAGATGT 
     4261 CTGAGATCCT TCCAGACGAA TCAAAATTCA ATATGTACCG GCTCTACTTT GGAAGCTCGT 
     4321 ATGAATCGGA GCTTGGAAAT GGGACCCCTT CTGACTTGAG CTCACACTGT CTGTCTGCAC 
     4381 CAAGCTCCGT GACTCAGGAT TTACCTGCAG CTCCTGGGAA AGACCCGTTA TTTACACAAC 
     4441 ATCCTGTGTT CAGGACACCT TCCCAAGAAG GCTGCCAAGA CCTCACCCAG GAGCAGAGAG 
     4501 ATCAGCTGAG GGCAGAGATC AGCATCATCA AAGGCAGATA CCGGAGCCAA AGTGGAGAGG 
     4561 ATGAATCCCT TAACCAGCCT GGACCAATCA AAACCACTTT TGCTATTAGC CAGGCACATT 
     4621 TAATGACTGC ACTTGCCCAC ACAAGACCGT CTATTAGTGA AGATGAAGGG AAGGAATTTG 
     4681 CTGAGCTGTA TGAGAACTTT CAAAATCCAA AGAAGAGAAA AAATCAAAGT GGAACAGTGT 
     4741 TTCGAACTGG ACAGAAAGTA ACTTTAGCAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//