LOCUS       MR211836                8601 bp    DNA     circular  SYN 
DEFINITION  Phka2 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 2 (Phka2), transcript variant 2
ACCESSION   NM_001177879
VERSION     NM_001177879.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4758..4849 
                     /label="Myc-DDK"  
     misc_feature    4879..5464 
                     /label="PolyA signal"  
     misc_feature    4936..4955 
                     /label="Restriction Enzyme"  
     misc_feature    5740..6412 
                     /label="ColE1"  
     misc_feature    6842..7636 
                     /label="Kan/Neo"  
     misc_feature    7671..7973 
                     /label="SV40 ori"  
     misc_feature    8059..8536 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4746 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="Phka2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCTTGGGC GAGATGCGGA GCAGGAGCAA TTCTGGGGTC CGCCTGGACG 
     1081 GGTACGCGAG GCTGGTGCAG CAAACCATCC TGTGTTACCA GAACCCAGTC ACTGGGCTGT 
     1141 TATCAGCCAG CCATGACCAG AAGGATGCCT GGGTACGGGA CAATATCTAT AGCATATTGG 
     1201 CTGTTTGGGG CCTGGGCATG GCCTACCGCA AGAATGCTGA CCGTGATGAG GATAAAGCCA 
     1261 AGGCCTATGA GTTGGAACAG AACGTAGTGA AGCTGATGCG AGGTCTTCTC CAGTGTATGA 
     1321 TGAGGCAGGT GGACAAAGTG GAGAAGTTCA AGCACACTCA GAGTACCAAG GACAGTCTAC 
     1381 ATGCCAAGTA CAACACTGCC ACATGTAGCA CTGTGGTGGG TGATGACCAG TGGGGCCACC 
     1441 TCCAGGTGGA TGCCACCTCT CTCTTCCTCC TGTTCCTGGC CCAGATGACA GCCTCAGGTT 
     1501 TGCGTATTAT TTTTACCCTT GACGAAGTGG CCTTCATACA GAATCTTGTC TTCTACATAG 
     1561 AAGCTGCATA TAAAGTTGCT GATTATGGGA TGTGGGAGCG TGGAGATAAA ACCAATCAGG 
     1621 GCATTCCGGA ACTGAATGCA AGCTCGGTGG GAGTGGCCAA GGCAGCACTT GAGGCCATTG 
     1681 ATGAACTAGA TCTTTTTGGA GCCCATGGAG GACGCAAATC GGTGATCCAC GTCCTACCTG 
     1741 ATGAAGTTGA GCACTGCCAG TCAATTCTTT TCTCCATGTT GCCAAGAGCA TCAACATCTA 
     1801 AAGAAATCGA TGCTGGACTT CTTTCTATTA TTTCTTTCCC GGCCTTTGCA GTAGAAGATG 
     1861 TGAACCTTGT GAATGTGACC AAAAATGAAA TTATTTCCAA GCTTCAGGGG CGGTATGGAT 
     1921 GCTGTCGCTT CCTTCGAGAT GGCTATAAAA CCCCAAGAGA GGATCCACAC CGATTGCATT 
     1981 ATGACCCTGC CGAACTTAAG CTCTTTGAAA ACATTGAATG TGAATGGCCT GTGTTCTGGA 
     2041 CTTATTTAAT CATAGATGGA ATCTTCAATG GTGATGCTGT TCAGGTCCAA GAATACCGAG 
     2101 AAGCCTTGGA GGGAATATTA ATCAGAGGCA AAGATGGGAT CCACTTGGTG CCAGAACTCT 
     2161 ATGCCATCCC ACCAGACAAG GTGGATGAAG AGTATAAGAA TCCACACACA GTAGACCGAG 
     2221 TTCCACTGGG AAAGCTGCCC CATCTTTGGG GACAGTCCTT GTACATCCTC AGCTCACTGC 
     2281 TAGCAGAGGG ATTCCTTGCC ACGGGTGAAA TCGATCCCTT AAATAGAAGA TTTTCTACTT 
     2341 CAGTCAAACC TGATGTTGTA GTGCAAGTTG CTGTTTTGGC AGAAAACAGT CACATTAAGG 
     2401 GGCTGTTGAA GGAGCATGGA ATGACTGTCC AGAGCATTGC TGATGTGCAT CCGATTCGAG 
     2461 TCCAGCCAGG CCGAATTCTT AGTCACATAT ATGCCAAACT TGGACGAAAT AAGAATATGA 
     2521 AATTGAGTGG TCGACCGTAT CGGCACATTG GTGTCCTTGG CACCTCTAAA CTCTATGTGA 
     2581 TTAGGAATCA CATCTTCACT TTTACACCCC AGTTCACTGA CCAGCATCAC TTCTACCTGG 
     2641 CCCTGGACAA TGAGATGATT GTGGAGATGC TGAGGATCGA ACTTGCCTAT CTGTGCACCT 
     2701 GCTGGCGGAT GACCGGCCGA CCCACTCTCA CCTTCCCTGT CACGCATACC ATGCTCACAA 
     2761 ATGATGGATC AGACATTCAT CCTGCAGTTC TTTCTACAAT TAGAAAACTA GAAGATGGCT 
     2821 ATTTTGGAGG TGCTAGAGTA AAACTAGGAA ATCTGGCAGA GTTCCTTACT ACCTCATTCT 
     2881 ACACACACCT GACCTTCCTG GATCCAGACT GCGATGAGAA GTTGTTTGGT GACATCACTG 
     2941 ATAGGAGCTT TAGTCCTGAC AGTGAGCCAG ACCTGGGAGG ATACCTGGAA GACAGCAGTC 
     3001 CTCAAGAAAG CCAAGATGAA CTTGACCAGT ATATCAGCCA CCTTCTTCAA AGCACATCCT 
     3061 TGAAGTGTTA CCTGCCCCCT CTTTGTAAGA AGTCAGAAGA CAGCCATTTT TTCAGTGCTA 
     3121 TTCACTCCAC TCGGGACATA CTTTCTGTGA TGGCCAAAGC GAAGGGTTTG GAAACTACAT 
     3181 TTTTTCCCAT GATTTTGCCA ACTAAAGTTC TAAGTGGACA CCGTAAGTCA CTGAATCTTG 
     3241 TTGACTCCCC TCAGCCACTC CTAAAGACGA CTCCTGAATA TGACTACCAG TGGCCCAGAG 
     3301 ACGACCATGA TGAAGTGGAC TGTGAGAAGC TAGTTGGGCA ACTGAAAGAC TGCTCAAACC 
     3361 TACAGGACCA AGCAGACATT CTGTACATTC TTTATGTAAT GAAGGGTCCC CGCTGGGATA 
     3421 CCAATTTGTT TGGGCAGCAT GGAGTCACTG TTCACAGTCT TCTCAGCGAG CTCTATGGAA 
     3481 AAGCTGGCCT AAACCAAGAA TGGAGTTTGA TTCGCTACAT TTCAGGCCTG CTCAGGAAGA 
     3541 AAGTGGAGGT CCTGGCTGAG GCCTGTGCAG ATCTGCTGTC CCACCAGAAG CAGCTTACAG 
     3601 TAGGACTGCC CCCTGAGCCC CGGGAGAAGA CCATATCTAC GCCTCTACCC CCAGAGGAGC 
     3661 TCACAGAACT CATATATGAA GCCAGTGGAC AGGACATCAG CATTGCTGTC CTCACACAGG 
     3721 AGATCGTGGT TTACCTGGCC ATGTATGTCC GGGCCCAGCC TAGCCTCTTT GCAGAGATGC 
     3781 TCAGACTCCG GATTGGATTG ATCATCCAGG TGATGGCCAC AGAGCTGGCT CGGAGCCTGA 
     3841 ACTGCTCAGG AAAAGAAGCT TCGGAGAGCT TGATGAACCT CAGCCCTTTC GACATGAAGA 
     3901 GCCTTCTGCA TCATATCCTG AGTGGGAAGG AGTTCGGTGT GGAGAGAAGC GTGCGCCCAA 
     3961 TACACTCCTC CATGTCCAGC CCTGCCATCT CCATCCATGA GGTGGGTCAT ACTGGAGCCA 
     4021 CCAAAACTGA GCGGAGTGGC ATCACCAGAC TGAGGAGTGA GATGAAACAG ATGAATAGGC 
     4081 GAGCTAGTGC TGATGAGCAG TTCTTTCCTT TGGGCCAGAC CATGTCCAAC AGTTTGCATT 
     4141 CCATCAAGTC TGTGAGGTCC AGCACCCCAT CCTCCCCGAC AGGCACATCA TCTACAGACT 
     4201 CTGGAGGACA GCACCTGGGC TGGGGAGAAC AGCAGGGCCA GTGGCTGCGC CGGAGAAGGT 
     4261 TGGATGGGGC CATTAACAGG GTCCCCGTGG GATTCTACCA GAAAGTGTGG AAGATCCTTC 
     4321 AGAAGTGCCA TGGCCTGTCC ATCGATGGTT ACGTCCTCCC ATCCTCGACA ACCCAAGAGA 
     4381 TGACCCCTTG TGAGATCAAG TTTGCCGTCC ATGTGGAGTC AGTGCTCAAC CGTGTGTCCC 
     4441 AGCCTGAGTA TCGGCAGCTA CTGGTAGAAG CCATCATGGT ACTGACTCTG CTCTCAGATA 
     4501 CCGAAATGGA CAGTATCGGG GGCATCATCC ATGTGGACCA GATAGTGCAG CTGGCCAATC 
     4561 AGCTGTTCCT GCAGGACCAG GTGTCATTTG GAACCACAGA CATCCTGGAA AAAGACCAAG 
     4621 CCACAGGAAT TTGCCACCTC TTTTATGACA GTGCTCCTAG TGGGGCTTAT GGTACAATGA 
     4681 CCTACCTAAC AAAAGCGGTC GCTTCTCATT TGCAGGAACT GCTGCCCAGT TCAGGCTGCC 
     4741 AGATGCAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4801 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4861 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4921 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4981 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5041 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5101 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5161 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5221 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5281 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5341 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5401 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5461 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5521 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5581 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5641 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5701 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5761 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5821 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5881 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5941 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6001 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6061 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6121 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6181 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6241 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6301 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6361 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6421 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6481 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6541 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6601 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6661 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6721 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6781 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6841 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6901 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6961 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7021 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7081 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7141 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7201 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7261 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7321 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7381 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7441 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7501 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7561 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7621 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7681 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7741 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7801 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8041 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8101 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8161 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8221 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8281 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8341 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8401 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8461 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8521 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8581 CAAAAATTTA ACGCGAATTT T
//