LOCUS       MR211834                8583 bp    DNA     circular  SYN 
DEFINITION  Plcb3 (Myc-DDK-tagged) - Mouse phospholipase C, beta 3 (Plcb3)
ACCESSION   NM_008874
VERSION     NM_008874.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4740..4831 
                     /label="Myc-DDK"  
     misc_feature    4861..5446 
                     /label="PolyA signal"  
     misc_feature    4918..4937 
                     /label="Restriction Enzyme"  
     misc_feature    5722..6394 
                     /label="ColE1"  
     misc_feature    6824..7618 
                     /label="Kan/Neo"  
     misc_feature    7653..7955 
                     /label="SV40 ori"  
     misc_feature    8041..8518 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4722..4728 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4722 
                     /label="Plcb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGCGCG AGGCCCGGCG TCCACGCGCT GCAGCTGGAG CCGCCCACCG 
     1081 TGGTGGAGAC CCTGCGGCGC GGGAGTAAGT TCATCAAATG GGACGAGGAG GCCTCCAGTC 
     1141 GGAACCTGGT GACCCTGCGT GTGGACCCTA ATGGCTTCTT CTTGTACTGG ACAGGACCCA 
     1201 ACATGGAGGT GGACACACTG GACATCAGCT CCATCAGGGA CACAAGGACA GGTCGTTATG 
     1261 CCCGCCTGCC CAAGGACCCT AAGATTCGAG AAGTACTGGG CTTTGGAGGT CCTGACACCC 
     1321 GGCTGGAGGA GAAACTGATG ACAGTGGTGG CCGGGCCAGA TCCAGTAAAT ACCACATTCT 
     1381 TGAACTTCAT GGCCGTGCAA GATGACACAG TCAAGGTCTG GTCAGAGGAG TTGTTTAAAC 
     1441 TGGCCATGAA CATATTGGCT CAGAACGCCT CCCGGAACAC CTTCCTGCGG AAAGCATACA 
     1501 CGAAGCTGAA GCTGCAGGTG AACCAGGATG GACGGATTCC GGTCAAGAAC ATTCTGAAGA 
     1561 TGTTCTCGGC GGACAAGAAG CGGGTGGAGA CGGCGCTGGA GTCCTGTGGC CTCAACTTCA 
     1621 ACCGAGTTGA GTCCATTCGC CCTGATGAGT TTCCCTTGGA AATTTTTGAG CGGTTCTTGA 
     1681 ATAAACTGTG TCTGCGCCCG GATATTGACA AGATCCTGCT GGAGATAGGT GCCAAGGGCA 
     1741 AGCCGTACCT CACTCTGGAG CAGCTCATGG ACTTTATCAA CCAGAAGCAG AGAGACCCGA 
     1801 GACTCAACGA AGTGCTGTAC CCGCCACTTC GGTCCTCACA GGCTCGGCTG CTCATTGAGA 
     1861 AGTACGAGAC GAACAAACAG TTCCTGGAGC GCGACCAGAT GTCTATGGAG GGCTTCAGCC 
     1921 GCTACCTGGG AGGGGAGGAG AATGGTATCC TGCCTCTGGA GGCCCTGGAT CTGAGCATGG 
     1981 ACATGACCCA GCCACTGAGC GCATATTTTA TCAACTCCTC ACACAACACC TATCTCACTG 
     2041 CGGGCCAGCT GGCTGGACCA TCATCGGTGG AGATGTACCG CCAGGCACTG CTGTGGGGCT 
     2101 GCCGCTGTGT GGAGCTGGAT GTATGGAAGG GACGGCCACC GGAGGAAGAG CCTTTCATCA 
     2161 CTCATGGTTT CACCATGACC ACTGAGGTGC CATTGCGTGA TGTGCTAGAG GCCATCGCTG 
     2221 AGGCAGCCTT CAAGACCTCG CCCTACCCTG TCATCCTCTC CTTTGAGAAC CATGTCGACT 
     2281 CGGCAAAGCA GCAGGCCAAG ATGGCTGAGT ACTGCCGCTC TATCTTTGGG GATGCGCTGC 
     2341 TCATTGACCC TCTCGACAAA TACCCGCTAT CTGCGGGCAT CCCCCTGCCT AGTCCACAGG 
     2401 ACCTGATGGG CCGTATCCTG GTGAAGAATA AGAAGCGACA TCGGCCCAGC ACAGGTGTCC 
     2461 CTGACAGCTC AGTGCGCAAG CGGCCTCTGG AACAGAGCAA CTCGGCCTTG AGTGAGAGCT 
     2521 CGGCCGCCAC AGAGCCCTCC TCACCTCAGC TTGGGTCCCC CAGCTCCGAC AGCTGCCCTG 
     2581 GCCTAAGCAA TGGGGAGGAG GTGGGACTCG AGAAGACCAG CCTGGAGCCT CAGAAGTCTC 
     2641 TAGGTGAGGA GAGCCTCAGC CGGGAACCTA ATGTGCCCAT GCCTGACCGT GACCGAGAGG 
     2701 ATGAGGAGGA AGATGAAGAA GAAGAGGAAA CAACGGATCC AAAAAAGCCC ACCACTGATG 
     2761 AGGGCACAGC CAGCAGTGAG GTCAATGCCA CGGAGGAGAT GTCAACGCTC GTCAACTACG 
     2821 TTGAGCCCGT CAAGTTCAAG TCCTTCGAGG CTGCTCGAAA AAGGAACAAG TGCTTCGAGA 
     2881 TGTCATCCTT CGTGGAGACC AAGGCAATGG AGCAACTGAC CAAGAGTCCT ATGGAGTTTG 
     2941 TGGAATACAA CAAACAGCAG CTCAGCCGCA TCTACCCTAA GGGCACACGA GTGGACTCCT 
     3001 CCAACTACAT GCCTCAGCTC TTCTGGAACG TGGGTTGCCA GCTTGTTGCC CTCAACTTCC 
     3061 AGACCTTGGA TTTGCCAATG CAGCTCAACG CAGGCGTGTT TGAGTACAAT GGGCGCAGCG 
     3121 GGTACTTGCT CAAGCCCGAG TTTATGCGGC GGCCGGACAA GTCTTTTGAT CCCTTCACTG 
     3181 AGGTCATCGT AGATGGCATA GTGGCCAACG CCTTGCGGGT CAAGGTGATT TCGGGGCAGT 
     3241 TCCTGTCTGA CAAGAAGGTG GGCATCTACG TCGAGGTGGA CATGTTTGGC CTCCCAGTTG 
     3301 ACACAAGACG CAAATATCGT ACCCGGACAT CCCAGGGGAA CTCATTCAAC CCTGTGTGGG 
     3361 ACGAGGAACC CTTTGACTTC CCCAAGGTTG TGCTGCCCAC GCTGGCCTCA CTTCGCATCG 
     3421 CAGCCTTTGA GGAGGGTGGC AAATTTGTTG GGCACCGTAT CCTGCCTGTC TCTGCTATCC 
     3481 GCTCAGGATA CCACTATGTC TGCCTGCGAA ACGAGGCCAA CCAACCTTTG TGCCTGCCTG 
     3541 CCCTGCTTAT CTACACTGAA GCTTCTGACT ACATCCCAGA TGACCACCAG GACTATGCGG 
     3601 AGGCCTTGAT TAACCCCATC AAGCACGTAA GCCTAATGGA CCAGCGGGCC AAGCAACTAG 
     3661 CCGCTCTCAT TGGGGAGAGT GAGGCTCAGG CCAGCACAGA GACATATCAG GAGACCCCGT 
     3721 GTCAACAGCC AGGGTCACAG CTCCCCTCCA ACCCCACACC TAACCCACTG GATGCCTCAC 
     3781 CTCGCTGGCC CCCCGGTCCT ACCACTTCCT CCACTAGCTC CTCCCTCAGT AGCCCAGGGC 
     3841 AGCGAGATGA TTTGATTGCC AGCATCCTCT CTGAGGTGAC CCCTACACCT CTAGAGGAGC 
     3901 TCAGAAGCCA CAAGGCTATG GTGAAGCTAC GGAGCCGGCA GGATCGAGAC CTGCGGGAGT 
     3961 TGCATAAGAA GCACCAGCGC AAGGCTGTGG CCCTCACCCG CCGCCTTCTG GATGGCCTGG 
     4021 CCCAGGCCAG GGCTGAGGGG AAGTGCCGGC CCTCCCCCAG TGCCCTGGGC AAGGCCACCA 
     4081 ACTCGGAGGA CGTGAAGGAG GAAGAGGAGG CCAAACAGTA TCGAGAGTTC CAGAACCGAC 
     4141 AAGTACAGAG CCTGTTGGAG CTGAGGGAAG CCCAGGCAGA TGTGGAGACC AAGCGGAAGC 
     4201 TGGAGCATCT ACGACAGGCT CATCAGCGGC TCAAGGAGGT TGTCCTGGAT ACACACACAA 
     4261 CACAGTTCAA GAGGCTGAAG GAGCTGAATG AAAGGGAGAA GAAGGAACTC CAGAAGATCC 
     4321 TGGACAGGAA GCGCAACAAC AGCATCTCAG AGGCCAAGAC AAGGGAGAAA CACAAGAAGG 
     4381 AGGTGGAACT GACAGAGATT AATCGGCGGC ACATCACTGA GTCGGTAAAC TCCATCAGAC 
     4441 GGCTGGAAGA GGCCCAGAAG CAGCGCCATG AACGCCTGGT GGCAGGGCAA CAGCAAGTCC 
     4501 TCCAGCAGCT AGAGGAAGAG GAACCCAAGC TGCTGGCCCA GCTGACCCAG GAGTGTCAGG 
     4561 AACAGCGAGA GAGGTTGCCC CAGGAGATCC GTCGGTGCCT GCTGGGCGAG ACAGCAGAGG 
     4621 GACTGGGGGA CGGCCCCCTG GTGGCCTGTG CCAGCAATGG TCATGCACCT GGGAGTGGTG 
     4681 GGCACCTGTC CAGCGCTGAC TCGGAGAGCC AGGAGGAGAA CACCCAGCTT ACGCGTACGC 
     4741 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4801 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4861 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4921 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4981 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5041 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5101 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5161 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5221 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5281 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5341 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5401 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5461 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5521 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5581 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5641 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5701 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5761 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5821 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5881 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5941 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6001 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6061 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6121 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6181 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6241 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6301 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6361 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6421 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6481 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6541 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6601 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6661 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6721 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6781 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6841 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6901 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6961 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7021 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7081 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7141 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7201 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7261 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7321 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7381 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7441 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7501 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7561 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7621 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7681 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7741 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7801 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7981 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8041 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8101 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8161 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8221 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8281 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8341 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8401 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8461 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8521 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8581 TTT
//