LOCUS       MR211819                8541 bp    DNA     circular  SYN 
DEFINITION  Evc2 (Myc-DDK-tagged) - Mouse Ellis van Creveld syndrome 2 homolog (human) (Evc2)
ACCESSION   NM_145920
VERSION     NM_145920.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4698..4789 
                     /label="Myc-DDK"  
     misc_feature    4819..5404 
                     /label="PolyA signal"  
     misc_feature    4876..4895 
                     /label="Restriction Enzyme"  
     misc_feature    5680..6352 
                     /label="ColE1"  
     misc_feature    6782..7576 
                     /label="Kan/Neo"  
     misc_feature    7611..7913 
                     /label="SV40 ori"  
     misc_feature    7999..8476 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4680..4686 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4680 
                     /label="Evc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCGACG GGCCCCACGG GCGCTGGGGG ACGAGCCACG TGGGTGCTGG 
     1081 CTGGGAATAT CCTGGCCGCT GCCCTGGTAC TGGGCTCGGG TCCGCGTGCA CTTCCACCAT 
     1141 CCTTCCCTGC CCTGGGCCCA GGGTCACCAT CTCGGCCCGG CCCAGCAGGA CCCTGGGCAA 
     1201 GCTCGCAGTA CAGCGATATT TCAAGAGAAG CCCGAGGTCC CTTTGAGAAT GGAGTGATAT 
     1261 TTCAAAAATG CTCACTGGTG TCTGGGCAGA GCGAATCGCA GACCATGCAC GTGCAGCTGT 
     1321 CCGTGAACAA CACCAGGACG CCCACATCAG TCAACCTTTC GAACCTGCTC GTGCTAGATG 
     1381 AGATCACTGG CCTTGCTGTG AAGGAGTCGC CTGGAAATAA TACTCAGGAT GGAATCCAGA 
     1441 CTTTCAGAAA GAGCTTTCTG CAAGTGGGAG AATGCTACTC TGTCAGCTAC ACAGCCTCGC 
     1501 TGGACCCCAC AGCCCTTGGG ACTGGGGAGA GCCTGGATCT TCCTGCCCGG CTCATCTTTC 
     1561 AGAGCCCCTC ACAGAACAGA ACTCAGCTAA AAGCCCCCTT TACCATCACA GTGGAAGAAA 
     1621 AGATAATGGT TCTTCCTAAC CATGGTCTTC ACGCAGCAGG CTTCATCGCA GCCTTCCTCA 
     1681 TCTCCCTCCT CCTGACCGTG GCAGCCCTGT TCTTCTTGGC GAGGGGTCGA TGTCTGCAGG 
     1741 GAGGCATGCT GTCCAGATGC CGGATTCAGC ATCCTGAGAA CAAGCTGGAG CCCTCGCCCT 
     1801 TCACCTCTGC TAACGGTGTG AGCCAGGACC TTTCCCTCAA TGACCAAGTG GTTGCCATCC 
     1861 TGACTTCTGA GGAGCCTGGG AGCATGCTTC AAGCCCTAGA AGAGTTGGAG ATCGCAACCC 
     1921 TGAATCAGGC AGATGCGGAT CTGGAGGCTT GTCGAAACCA GATTAGTAAG GACATCATTG 
     1981 CCCTTTTGAT GAAGAATCTG GTCAGCGGTG GTCACCTCTC TCCGCAAACA GAAAGGAAGA 
     2041 TGGCCGCTGC TTTCAAAAAG CAGTTTCTGC TGTTGGAAAA TGAAATACAG GAGGAGTATG 
     2101 AGCGGAAGAT GTTGGCACTG ACGGCAGAGT GTGACCTAGA GATGAGGAAG AAGACAGAAA 
     2161 ACCAGTACCA GAGGGAGATG GTGGCCATGG AGGAAGCAGA AGAGGTGTTG AAGAGAGTTA 
     2221 GCGAGAGGTC TGCTGCCGAG TGCAGCAGCC TTTTGCGGAC CCTCCATGGG CTGGAGCAGG 
     2281 AGGACATGCA GAGGTCCCTT ACTCTGGACC AAGCCGAGGA CTTTGCTCAA GCACACAGGC 
     2341 AGCTGGCTGT TTTCCAGAGG AATGAATTAC ACAGCATCGT CTATACCCAG ATACAGAGTG 
     2401 CTGTCTCCAA AGGGGAACTG AGGCCTGAGG TGGCTAAAAT GATGCTGCAA GACTACTCTA 
     2461 AAACACAGGA GAGTGTAGAA GAGCTGATGG ACTTCTTCCA GGCCACCAAG CGATACCATC 
     2521 TCAGCAAGAG GTTTGGCCAC AGAGAGTACC TGGTCCAGAG ACTGCAGGCC ATGGAGACCC 
     2581 GGGTGCAAGG GCTGCTGAAC ACCGCGGCTA CCCAGCTAAC AAGCCTCATC CATAAACATG 
     2641 AGAGGGCTGG CTACTTGGAT GAAGATCAGA TGGAAACGCT ATTGGAGCGT GCACAGACAG 
     2701 AAACCTTTTC TATAAAACAG AAGCTGGACA ATGACTTAAA GCAGGAAAAG AAAAGGCTCC 
     2761 ACCAGAGATT AATAACCAGG AGGCGGCGAG AGCTTCTGCA GAAGCACAAG GAGCAGCAGA 
     2821 AGGAGCAGGT GTCCCTTGGC GAGGCCTCCA GCACCGCGGA GGACGCAGTC CAGTACCTGC 
     2881 ACCAGTGGAG GAGCGTGATG GCCGAGCACA CCGCTGCCCT GGAGGAGCTG CAGGAACGTC 
     2941 TGGACCAGGC TGCCCTGGAT GACCTCCGGG TCCTGACTGT GTCGCTGTCG GAGAAAGCCA 
     3001 CGGAAGAGCT GAGGCGCCTG CAGAGCACGG CCATGACACA GGAGCTGCTG AAGCGCAGTG 
     3061 CTCCCTGGCT CTTCCTGCAG CAGATCTTAG AGGAGCACAG CCGGGAGTCT GCGGCTCGCA 
     3121 CCACGCAACT GGAGGCAGAG GAGAGGGAAC GTGGCCAGGA GCTGGTGCAG GGCGTGAGGC 
     3181 AGAGGCTACA GCAGGATGCA CTGGAGGCCT ACACGGAAGA GCAGGCAGAG CTGAGGCACT 
     3241 GGGAGCACTT GGTGTTCATG AAACTCTGCT GTGCGGCCAT CTCATTGTCT GAAGAAGACC 
     3301 TGCTCCGGGT AAGGCAGGAG GCACAGGGCT GCTTCAGCCA GCTGGACCGG AGCTTGGCCC 
     3361 TCCCCAGAGT CCGTGCACGA GTGTTGCAGC AGCAGGCGCA GATGGCCTGG CGGGAAGCAG 
     3421 AGTTTCGGAA ACTGGACCAG GCCCTGGCTG CGCCTGAGCT GCAGTCCAAG GCAAGAAAGC 
     3481 TGCGTTCCAA GGGCAGAGGC AAGGCCGATC TTCTGAAGAA GAACCTGGAG GATAAGATCC 
     3541 GACTATTCGA GGAGCGGGCC CCTGTAGAAC TGGCTGACCA GGTGCGAGGG GAATTGCTCC 
     3601 AAGAGAGGGT CCAGAGGCTG GAGGCCCAGG AGGCGCACTT TGCTGAGTCA CTTGTGGCTC 
     3661 TGCAGTTCCA GAAGGTGGCC CGGGCTGCTG AGACCCTCTC AGTCTACACC GCCCTGCTCA 
     3721 GCATTCAAGA CCTGCTGCTG GGGGAGCTGA GTGAGTCGGA GACCCTGACC AAGTCAGCCT 
     3781 GTGTGCAGAT CCTGGAGTCC CACAGGCCGG AGCTGCAGGA GCTGCAGGAG CTGGAGAGGA 
     3841 AGCTGGAGGA CCAGCTGGTG CAGCAGGAAG AGGCCGAGCA GCAGCGGGTC CTGGAGAGCT 
     3901 GGCAGCGGTG GGCGGCCGAT GGACCTGGGC TGAGCGAACC TGAGGAGATG GATCCTGAAA 
     3961 GGCAGGTCTC TGCCATCCTG CGGCAAGCCC TGAACAAGGG CCAGAAGCTG CTGGAGCAGC 
     4021 ATCAGCAGAG GGTGAGAGAG GAGTGGCAGA ACGGTGCGGT GCTGGAAGAT TCTCTGGAAA 
     4081 GCATTGAAGC TGACACCATG GCCAGCCTTT GCAGCCAGGG ACTGAGGCTG GTGTCATACC 
     4141 TCTCAAGGAT GACAATGGTA CCAGGCAGCA CACTACTGCG ACTCCTGAGT GTGGTGCTTC 
     4201 CAGCGGCTTC ACAGCCTCAG CTACTGGCCC TGCTGGACGC AGTCAGTGAG AAGCACTCCG 
     4261 ACCACACAGC TGAGAATGAG AGCAGTGGTG AGCAGGCTCA GGCAGAGCAG AGCAAGAGGA 
     4321 GGAAACACCA AGTTTGGTGG AAGGTTTTGG ACAGCAGATT CAGAGCAGAT CTGGTGAGTC 
     4381 AAGGACTGGA GAGGATGCTC TGGGCCCGCC AGAAGAAGGA GCGCATATTG AAGAAGATAT 
     4441 ATGTTCCCGT CCAGGAGAGG GTGATGTTTC CTGGGAAAGG AAGCTGGCCA CACCTGTCCC 
     4501 TAGAACCCAT TGGAGAACTG GCTCCCATCC CCATCACTGG GGCAGACGCT ATGGACATAT 
     4561 TAAACACGGG AGAAAAGATC TTTGTATTCA GAAGCCCAAG GGAGCCCGAG ATCTCTTTGC 
     4621 GTGTTCCTCC CAGGAGGAAA AAGAATTTCC TGAATGCCAA GAAGGCCAAC AGGGCCTTGG 
     4681 GCTTGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//