LOCUS       MR211813                8529 bp    DNA     circular  SYN 
DEFINITION  Smc4 (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 4 (cDNA clone MGC:70057 IMAGE:6512832)
ACCESSION   BC061481
VERSION     BC061481.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Smc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGTGAA GCTGGAGCTC CTCGTCTCAT GATAACTCAT ATTGTAAACC 
     1081 AAAACTTCAA GTCCTATGCT GGAGAGAAAG TTCTGGGACC TTTCCATAAG CGCTTTTCCT 
     1141 GTATTATTGG ACCAAATGGC AGTGGAAAAT CCAATGTTAT TGATTCCATG CTTTTTGTGT 
     1201 TTGGCTATCG AGCACAAAAA ATAAGGTCTA AAAAGCTATC GGTACTGATA CATAATTCTG 
     1261 ATGAACACAA GGATATTCAG AGTTGTACTG TAGAAGTTCA TTTTCAAAAG ATAATTGATA 
     1321 AGGAAGGAGA TGATTACGAA GTCCTTCCTA ACAGCAACTT CTATGTATCC AGAACCGCCT 
     1381 ACAGAGATAG CACTTCTGTC TACCACATAA GTGGGAAGAA GAAGACGTTT AAAGATGTTG 
     1441 GAAATCTCCT TAGAAGCCAT GGCATTGACT TAGACCATAA TCGATTTTTA ATCTTGCAGG 
     1501 GTGAAGTTGA ACAGATTGCT ATGATGAAAC CCAAAGGCCA GACTGAACAT GATGAAGGTA 
     1561 TGCTTGAGTA TTTAGAAGAT ATAATTGGCT GTGGACGGCT AAATGAACCT ATTAAAGTCT 
     1621 TGTGTCGGAG AGTTGAAATA TTAAATGAAC ACAGAGGAGA AAAGTTAAAT AGAGTGAAAA 
     1681 TGGTAGAAAA GGAGAAGGAT GCACTAGAGG GAGAGAAAAA CATAGCCATT GAGTTCCTTA 
     1741 CCCTGGAAAA TGAAATGTTT AAGAAAAAGA ATCACATCTG TCAATACTAC ATTTATGATC 
     1801 TGCAGAATAG AATTGCTGAA ATAACGACTC AAAAGGAAAA AATTCATGAA GATACTAAAG 
     1861 AAATTACTGA GAAGAGCAAT GTGCTTTCCA ATGAAATGAA AGCGAAGAAT TCAGCTGTAA 
     1921 AGGATGTAGA AAAGAAGCTC AATAAAGTTA CAAAATTTAT CGAACAGAAT AAAGAAAAAT 
     1981 TTACACAACT AGATTTGGAA GATGTTCAAG TTAGGGAAAA ATTAAAACAT GCTACAAGTA 
     2041 AAGCCAAGAA ACTGGAGAAA CAACTTCAAA AAGATAAAGA AAAGGTTGAA GAGCTGAAAA 
     2101 GTGTACCTGC CAAGAGTAAG ACTGTCATAA ATGAGACAAC AACTAGAAAC AATTCGCTTG 
     2161 AGAAAGAAAG AGAGAAAGAA GAAAAAAAAT TAAAAGAGGT CATGGACAGC CTTAAGCAGG 
     2221 AAACACAGGG GCTTCAGAAA GAAAAGGAGA TTCAAGAAAA GGAACTTATG GGTTTTAACA 
     2281 AATCAGTAAA TGAAGCACGT TCAAAGATGG AAGTTGCTCA GTCAGAACTT GATATCTATC 
     2341 TCAGTCGACA TAATACAGCA GTGTCTCAAT TAAGTAAGGC GAAGGAAGCC TTAATTACAG 
     2401 CTTCTGAGAC TCTCAAGGAG AGGAAAGCTG CAATCAAGGA TATAAACACA AAGCTCCCTC 
     2461 AGACGCAACA GGAACTAAAG GAGAAAGAAA AAGAACTTCA GAAACTGACA CAAGAAGAAA 
     2521 TAAACTTAAA AAGTTTGGTT CATGATCTTT TCCAAAAAGT TGAAGAAGCA AAGAGTTCCT 
     2581 TAGCAATGAA TCGAAGTAGG GGGAAAGTAC TTGATGCAAT AATTCAAGAA AAAAAATCAG 
     2641 GCAGGATCCC AGGAATATAT GGAAGATTGG GAGACTTAGG AGCTATTGAT GAAAAATATG 
     2701 ACATTGCTAT TTCATCCTGT TGCCATGCAT TGGACTACAT TGTTGTTGAT TCTATTGATA 
     2761 CAGCCCAAGA ATGTGTAAAC TTCCTTAAAA AGCATAATAT TGGGATTGCA ACTTTCATAG 
     2821 GTTTGGATAA GATGACAGTG TGGGCCAAAA AAATGAGCAA AATTCAAACT CCTGAAAATA 
     2881 CTCCTCGGTT ATTTGATCTA GTTAAAGTGA AAAATGAGGA AATCCGCCAA GCCTTTTACT 
     2941 TTGCTTTACG AGATACCTTG GTGGCTAACA ATTTGGATCA AGCTACAAGA GTAGCGTACC 
     3001 AAAGAGACAG ACGCTGGAGA GTAGTGACTC TGCAGGGACA GATCATAGAA CAGTCAGGTA 
     3061 CAATGAGTGG CGGTGGAAGC AAGGTGATGA GAGGAAGGAT GGGCTCTTCG GTTATCGATG 
     3121 AAATCTCTGT AGAAGAGGTA AATAAGATGG AATCCCAGTT GGAAAGGCAT TCTAAACAAG 
     3181 CAATGCAAAT CCAAGAACAG AAAGTACAAC ATGAAGAAGC AGTAGTTAAG TTACGGCACA 
     3241 GTGAACGAGA CATGAGAAAT ACACTGGAAA AGTTCGCTGC AAGCATCCAG GGCTTATCAG 
     3301 AGCAAGAAGA GTATCTGTGT GTACAAATTA AAGAACTGGA AGCTAACGTT CTTACTACGG 
     3361 CCCCTGACAG AAAACAACAG AAACTGCTGG AAGAAAATGT CAGTGTTTTC AAAAAAGAAT 
     3421 ATGATGCTGT GGCTGAGAAA GCTGGTAAAG TTGAAGCTGA GATTAAACGG CTACACAATA 
     3481 CCATCATAGA TATCAACAAC CGAAAACTCA AGGCCCAGCA AAACAAACTT GACACGATAA 
     3541 ATAAGCAGCT TGATGAATGT GCTTCTGCTA TTACTAAGGC CCAAGTAGCA ATCAAGACTG 
     3601 CTGACAGAAA TCTTAAAAAG GCACAGGATT CTGTTTGCCG CACAGAGAAA GAAATAAAAG 
     3661 ACACTGAAAA AGAAATAAAT GACTTAAAGA CAGAGCTAAA AAATATTGAA GACAAGGCAG 
     3721 AAGAGGTCAT AAACAATACA AAAACTGCAG AGACATCCTT ACCAGAAATC CAGAAAGAAC 
     3781 ATCGTAATCT ACTTCAGGAA CTAAAAGTTA TTCAAGAAAA TGAACATGCT CTTCAGAAAG 
     3841 ATGCACTTAG TATTAAGTTG AAACTTGAAC AGATAGATGG CCACATTTCT GAACATAACT 
     3901 CAAAAATAAA ATACTGGCAA AAAGAGATTT CAAAAATAAA ATTGCATCCT GTTGAAGATA 
     3961 ATCCTGTTGA GACAGTAGCA GTTCTAAGCC AGGAGGAGCT TGAGGCAATC AAGAATCCAG 
     4021 AGTCTATAAC AAATGAAATT GCACTTTTGG AAGCTCAGTG TCGTGAAATG AAACCAAACC 
     4081 TTGGAGCCAT CGCTGAGTAT AAAAAAAAGG AAGATTTATA TTTGCAAAGA GTAGCCGAAC 
     4141 TGGACAAAAT TACTTCTGAA AGAGATAATT TTAGACAAGC ATATGAAGAT CTTCGAAAAC 
     4201 AAAGGCTGAA TGAATTTATG GCTGGTTTTT ACGTAATAAC AAATAAACTA AAAGAAAACT 
     4261 ACCAGATGCT CACATTGGGA GGAGATGCTG AACTGGAGCT TGTGGACAGT TTAGATCCTT 
     4321 TTTCTGAAGG AATCATGTTC AGTGTTCGGC CACCTAAGAA AAGTTGGAAG AAGATCTTTA 
     4381 ACCTCTCAGG AGGCGAGAAA ACCCTTAGTT CCTTGGCCTT AGTGTTTGCT CTTCATCACT 
     4441 ACAAGCCCAC TCCCCTCTAC TTCATGGATG AGATTGATGC AGCTCTGGAT TTTAAAAATG 
     4501 TGTCTATTGT TGCATTTTAT ATATATGAGC AAACAAAAAA TGCCCAGTTC ATAATAATCT 
     4561 CTCTTCGAAA TAATATGTTT GAGATTTCAG ACAGACTTAT TGGCATCTAT AAAACATATA 
     4621 ATAGTACAAA AAGTGTTGCA GTGAACCCAA AACAAATTGC GTCTAAAGGA CTCTGTACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//