LOCUS       MR211812                8529 bp    DNA     circular  SYN 
DEFINITION  Plcb1 (Myc-DDK-tagged) - Mouse phospholipase C, beta 1 (Plcb1), transcript variant 1
ACCESSION   NM_001145830
VERSION     NM_001145830.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Plcb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGGGCT CAGCCCGGAG TGCACGCCTT GCAACTCAAG CCCGTGTGCG 
     1081 TGTCCGACAG CCTCAAGAAG GGCACCAAAT TCGTCAAGTG GGATGATGAC TCCACTATTG 
     1141 TTACTCCAAT TATTTTGAGG ACTGATCCTC AGGGATTTTT CTTTTACTGG ACAGACCAGA 
     1201 ATAAGGAGAC GGAGCTGTTA GATCTCAGCC TTGTCAAGGA TGCCAGGTGT GGGAAGCACG 
     1261 CCAAAGCTCC CAAGGACCCC AAGTTGCGTG AACTTCTGGA TGTTGGGAAC ATCGGACACT 
     1321 TGGAACAGCG CATGATAACT GTGGTGTATG GACCAGACCT GGTGAACATT TCCCACTTGA 
     1381 ATCTTGTGGC TTTTCAAGAG GAAGTAGCCA AGGAATGGAC AAATGAGGTT TTCAGCTTGG 
     1441 CAACAAACCT GCTGGCTCAG AACATGTCCA GGGACGCATT TCTGGAGAAA GCATATACTA 
     1501 AGCTCAAGCT CCAGGTCACC CCAGAAGGCC GCATTCCTCT TAAAAACATC TATCGATTGT 
     1561 TCTCAGCAGA CCGGAAGCGC GTTGAAACTG CACTAGAGGC TTGTAGTCTT CCATCTTCGA 
     1621 GGAATGACTC CATTCCCCAA GAGGACTTCA CTCCAGATGT ATACAGAGTT TTCCTGAACA 
     1681 ATCTCTGTCC CCGACCTGAA ATTGATAACA TCTTTTCTGA ATTTGGTGCC AAAAGCAAAC 
     1741 CGTACCTGAC GGTTGATCAG ATGATGGATT TTATCAACCT TAAGCAGAGA GATCCCCGGC 
     1801 TCAATGAAAT CCTTTACCCA CCTCTGAAGC AGGAGCAGGT CCAAGTGTTG ATTGAGAAAT 
     1861 ATGAGCCCAA CAGCAGCCTC GCCAAGAAAG GGCAGATGTC AGTGGATGGA TTCATGCGCT 
     1921 ACCTGAGCGG AGAAGAAAAT GGAGTCGTTT CACCTGAGAA ACTGGATTTG AACGAAGACA 
     1981 TGTCTCAGCC CCTGTCTCAC TATTTCATCA ATTCCTCGCA CAACACCTAC CTCACAGCTG 
     2041 GCCAGTTGGC TGGGAACTCG TCTGTAGAGA TGTATCGCCA GGTGCTTCTG TCTGGATGTC 
     2101 GCTGTGTGGA GCTGGACTGC TGGAAGGGAA GGACCGCTGA GGAAGAGCCT GTCATTACCC 
     2161 ATGGATTCAC CATGACAACA GAAATATCCT TCAAGGAAGT CATAGAAGCC ATCGCAGAGT 
     2221 GTGCGTTCAA GACGTCTCCT TTCCCCATCC TCCTTTCCTT TGAGAACCAC GTGGATTCCC 
     2281 CGAAGCAACA AGCCAAGATG GCCGAGTATT GCCGATTAAT CTTTGGTGAT GCCCTCCTGA 
     2341 TGGAACCACT GGAAAAATAC CCACTGGAAT CTGGGGTACC TCTTCCAAGC CCTATGGATT 
     2401 TAATGTATAA AATCTTGGTG AAAAACAAGA AGAAGTCGCA CAAGTCGTCA GAGGGAAGTG 
     2461 GTAAGAAGAA GCTTTCAGAG CAAGCTTCCA ACACCTACAG CGACTCCTCC AGTGTGTTCG 
     2521 AGCCTTCGTC TCCAGGAGCT GGGGAAGCAG ATACGGAGAG TGATGACGAT GACGACGACG 
     2581 ATGACTGTAA AAAGTCTTCC ATGGATGAGG GGACAGCTGG CAGCGAAGCC ATGGCCACAG 
     2641 AAGAGATGTC TAACCTGGTG AACTATATTC AGCCTGTCAA GTTTGAGTCC TTTGAAATTT 
     2701 CAAAAAAAAG AAATAAAAGC TTTGAAATGT CTTCCTTCGT GGAAACCAAA GGACTTGAAC 
     2761 AACTTACAAA GTCTCCGGTG GAATTTGTAG AATACAACAA GATGCAACTT AGCAGGATAT 
     2821 ATCCCAAGGG AACACGTGTG GATTCATCCA ACTACATGCC TCAACTCTTC TGGAATGCTG 
     2881 GCTGTCAGAT GGTGGCGCTC AACTTCCAGA CAGTGGATCT AGCTATGCAG ATAAACATGG 
     2941 GCATGTATGA GTACAATGGG AAGAGCGGCT ATAGGCTGAA GCCAGAGTTC ATGAGGAGGC 
     3001 CGGACAAGCA TTTTGATCCA TTTACTGAAG GAATCGTAGA TGGGATAGTG GCCAACACTC 
     3061 TATCTGTTAA GATTATTTCA GGTCAGTTCC TCTCTGATAA GAAAGTTGGG ACTTATGTGG 
     3121 AAGTGGATAT GTTTGGCTTG CCTGTGGACA CAAGGAGGAA GGCATTTAAA ACCAAGACAT 
     3181 CCCAAGGAAA TGCTGTAAAC CCTGTCTGGG AAGAAGAACC TATTGTATTC AAAAAGGTAG 
     3241 TTCTACCTTC TCTGGCCTGT TTGAGGATAG CAGCATATGA AGAGGGAGGC AAATTTATTG 
     3301 GCCACCGGAT ATTGCCTGTG CAGGCTATTC GGCCAGGCTA TCACTACATC TGCCTGCGGA 
     3361 ATGAGAGGAA CCAGCCCCTG ACGCTGCCAG CTGTCTTTGT CTACATAGAA GTCAAAGATT 
     3421 ATGTGCCAGA CACGTATGCA GATGTAATCG AAGCTCTATC AAACCCAATC CGATATGTCA 
     3481 ATCTGATGGA ACAGAGAGCT AAGCAGCTGG CTGCGTTGAC CCTGGAGGAT GAAGAGGAAG 
     3541 TAAAGAAGGA GGCTGACCCT GGAGAAACAT CTTCCGAGGC TCCAAGCGAA ACCAGGACAA 
     3601 CGCCAGCAGA GAATGGGGTG AATCACACCG CATCCCTTGC ACCCAAGCCG CCTTCCCAGG 
     3661 CTCCACACAG CCAGCCTGCT CCAGGGTCTG TGAAGGCACC TGCCAAAACA GAAGATCTGA 
     3721 TCCAGAGCGT GTTAACAGAA GTAGAGGCGC AAACCATCGA AGAGCTCAAG CAACAGAAAT 
     3781 CGTTCGTGAA ACTTCAAAAG AAACACTACA AAGAAATGAA AGACCTGGTG AAGAGACACC 
     3841 ACAAGAAAAC CACGGAGCTC ATTAAGGAGC ACACGACCAA GTACAACGAG ATTCAGAATG 
     3901 ACTACCTGAG AAGGAGGGCA GCCTTGGAGA AGTCCGCCAA AAAGGACAGC AAGAAGAAAT 
     3961 CTGAACCCAG CAGCCCTGAC CACGGCTCAT CAGCCATTGA GCAAGACCTC GCTGCCCTGG 
     4021 ATGCAGAAAT GACTCAGAAG TTGATAGACT TGAAAGACAA GCAACAACAG CAGCTGCTTA 
     4081 ATCTTCGGCA AGAGCAGTAT TACAGCGAGA AGTACCAAAA GCGAGAACAC ATTAAACTGC 
     4141 TTATTCAGAA GTTGACAGAT GTTGCAGAAG AGTGTCAGAA CAATCAGTTA AAGAAGCTGA 
     4201 AGGAAATCTG CGAGAAAGAG AAGAAGGAAT TAAAGAAGAA AATGGATAAG AAGAGGCAAG 
     4261 AGAAGATAAC AGAAGCCAAG TCCAAAGACA AAAGCCAGAT GGAAGAGGAG AAGACAGAGA 
     4321 TGATCCGATC GTACATCCAG GAGGTGGTTC AGTACATCAA GAGGTTAGAG GAAGCACAAA 
     4381 GTAAAAGACA AGAAAAACTC GTGGAGAAAC ACAATGAGAT CCGCCAGCAG ATCCTCGATG 
     4441 AGAAGCCCAA GCTGCAGACG GAGCTGGAGC AAGAATACCA AGACAAGTTC AAAAGGCTGC 
     4501 CCCTGGAGAT TCTGGAGTTT GTACAGGAAG CCATGAAAGG GAAGATCAGC GAGGACAGCA 
     4561 ATCACGGCTC TGCCCCTCCC TCTCTGGCCT CAGATGCTGC AAAGGTGAAC CTCAAGTCTC 
     4621 CCTCCAGTGA GGAGATAGAA AGAGAGAACC CAGGAAGAGA GTTTGATACT CCTCTGACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//