LOCUS       MR211808                8520 bp    DNA     circular  SYN 
DEFINITION  Trpm4 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4)
ACCESSION   NM_175130
VERSION     NM_175130.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4677..4768 
                     /label="Myc-DDK"  
     misc_feature    4798..5383 
                     /label="PolyA signal"  
     misc_feature    4855..4874 
                     /label="Restriction Enzyme"  
     misc_feature    5659..6331 
                     /label="ColE1"  
     misc_feature    6761..7555 
                     /label="Kan/Neo"  
     misc_feature    7590..7892 
                     /label="SV40 ori"  
     misc_feature    7978..8455 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4659..4665 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4659 
                     /label="Trpm4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGGGCCG GAGAAGGAGC AGAGCTGGAT CCCTAAGATC TTCAGGAAGA 
     1081 AGGTGTGCAC GACGTTCATA GTGGACCTCT CCGATGATGC GGGAGGGACC TTGTGCCAGT 
     1141 GTGGGCAACC TCGGGATGCC CACCCTTCTG TGGCTGTGGA GGATGCCTTT GGGGCAGCCG 
     1201 TAGTTACCGA GTGGAACAGT GATGAGCACA CCACGGAGAA GCCCACAGAT GCCTATGGGG 
     1261 ACCTGGACTT CACGTACTCT GGCCGAAAGC ACAGCAACTT TCTCCGGCTG TCTGACCGCA 
     1321 CGGATCCGGC CACTGTGTAC AGTCTCGTTA CCCGCTCTTG GGGCTTTCGT GCCCCAAACT 
     1381 TGGTGGTGTC GGTGCTGGGG GGGTCGGGGG GCCCCGTGCT CCAGACCTGG CTGCAGGACC 
     1441 TTCTGCGTCG CGGGCTGGTG AGAGCCGCCC AGAGCACAGG GGCCTGGATC GTCACTGGGG 
     1501 GACTGCACAC AGGCATTGGC CGGCACGTGG GTGTGGCTGT AAGGGACCAC CAGACAGCCA 
     1561 GCACTGGGAG CAGCAAGGTG GTGGCCATGG GTGTGGCCCC CTGGGGAGTG GTCCGGAACA 
     1621 GAGACATGCT GATCAACCCC AAGGGCTCAT TCCCCGCAAG GTACCGGTGG CGTGGTGACC 
     1681 CTGAGGATGG TGTTGAGTTC CCCCTGGACT ATAACTATTC TGCTTTCTTC TTGGTGGATG 
     1741 ATGGCACCTA TGGCCGCCTG GGTGGTGAGA ACCGCTTCCG CCTTCGGTTT GAGTCCTATG 
     1801 TGGCTCAGCA GAAGACCGGA GTGGGAGGGA CTGGAATTGA CATCCCAGTG CTCCTTCTTC 
     1861 TCATCGATGG AGATGAGAAG ATGTTAAAGC GGATAGAGGA TGCCACCCAA GCTCAGCTCC 
     1921 CCTGCCTCTT GGTGGCGGGC TCTGGGGGTG CTGCAGACTG TCTGGTGGAG ACCCTGGAAG 
     1981 ATACTCTGGC CCCAGGGAGT GGGGGACTTA GGCGAGGCGA AGCCCGGGAT CGGATTAGGC 
     2041 GATACTTCCC TAAAGGAGAC CCTGAGGTTC TTCAGGCCCA GGTAGAGAGG ATCATGACCC 
     2101 GAAAGGAGTT GCTGACGGTC TATTCATCAG AAGACGGCTC CGAGGAGTTT GAGACTATCG 
     2161 TTTTGAGGGC TCTTGTGAAA GCCTGTGGGA GCTCTGAGGC CTCAGCCTAC CTGGATGAGC 
     2221 TGCGTTTGGC TGTGGCTTGG AACCGCGTGG ACATCGCCCA AAGTGAACTT TTCCGTGGGG 
     2281 ACATCCAATG GCGGTCCTTC CACCTGGAGG CATCTCTCAT GGATGCTCTG CTGAATGACC 
     2341 GGCCTGAATT TGTGCGCCTG CTCATCTCCC ATGGCCTTAG CCTGGGACAC TTCCTGACCC 
     2401 CCGTGCGTCT GGCCCAGCTG TACAGCGCGG TGTCCCCTAA CTCACTGATC CGAAATCTTC 
     2461 TGGACCAGGC GTCCCATGCT AGTAGCAGCA AATCCCCTCC TGTAAATGGA ACTGTAGAGC 
     2521 TCCGGCCTCC CAATGTGGGG CAAGTGCTGA GGACTCTGTT GGGAGAAACG TGTGCACCAC 
     2581 GGTACCCTGC CAGGAACACC CGAGACTCCT ATCTGGGCCA GGACCACAGA GAGAATGACT 
     2641 CTCTGCTTAT GGACTGGGCC AACAAGCAAC CATCAACGGA TGCAAGCTTT GAACAAGCCC 
     2701 CCTGGAGTGA CCTGCTTATT TGGGCTCTGT TGCTGAACCG GGCCCAGATG GCTATCTATT 
     2761 TCTGGGAGAA GGGCTCCAAC TCAGTGGCCT CTGCTCTTGG GGCTTGTCTT TTACTCCGAG 
     2821 TGATGGCTCG CCTAGAGTCG GAGGCTGAGG AGGCCGCGCG GCGGAAGGAT CTTGCTGCCA 
     2881 CGTTTGAAAG CATGAGCGTG GACCTCTTTG GAGAGTGTTA CCACAACAGT GAAGAGCGAG 
     2941 CAGCCCGCCT TCTTCTGCGC CGCTGTCCCC TCTGGGGAGA GGCCACCTGC CTCCAGCTTG 
     3001 CCATGCAGGC TGATGCCCGA GCGTTCTTTG CCCAGGATGG AGTCCAGTCT CTTCTGACAC 
     3061 AGAAGTGGTG GGGGGAGATG GACAGCACAA CTCCCATCTG GGCCCTGCTT CTCGCCTTCT 
     3121 TCTGCCCGCC TCTCATCTAC ACCAACCTTA TCGTCTTCAG GAAGTCAGAG GAGGAGCCCA 
     3181 CACAGAAGGA TCTTGACTTT GATATGGACA GCAGCATCAA TGGAGCAGGT CCTCCTGGGA 
     3241 CTGTAGAACC CTCTGCAAAG GTGGCCCTTG AGAGGCGGCA GCGGCGACGG CCAGGACGTG 
     3301 CCCTCTGCTG TGGCAAGTTC TCCAAGCGCT GGTCGGACTT CTGGGGTGCC CCAGTGACTG 
     3361 CCTTCCTGGG TAATGTGGTC AGTTACCTGC TGTTCCTGCT GCTGTTTGCC CACGTGTTGC 
     3421 TGGTGGATTT CCAGCCCACA AAGCCAAGTG TCTCCGAGCT GCTGCTGTAC TTCTGGGCCT 
     3481 TCACGTTGCT GTGCGAGGAG CTGCGCCAGG GCCTGGGTGG TGGCTGGGGT AGCCTGGCCA 
     3541 GTGGGGGACG TGGTCCGGAC AGAGCTCCCC TGCGCCATCG GCTGCACCTC TACCTCTCAG 
     3601 ATACCTGGAA CCAATGTGAC CTGCTGGCAC TCACCTGCTT CCTGCTGGGC GTAGGCTGCC 
     3661 GGCTGACCCC TGGGCTCTTT GACTTGGGAC GCACCGTCCT CTGCCTTGAC TTCATGATCT 
     3721 TCACACTGCG CCTGCTGCAC ATCTTCACGG TGAACAAGCA GCTGGGGCCC AAGATTGTCA 
     3781 TAGTGAGCAA AATGATGAAG GATGTATTCT TCTTCCTCTT CTTCCTCTGC GTGTGGCTTG 
     3841 TAGCTTATGG GGTGGCCACA GAGGGGATCC TGAGGCCCCA GGACCGCAGT CTACCGAGTA 
     3901 TCCTGCGCAG GGTCTTCTAC CGACCGTATC TGCAGATCTT CGGACAAATT CCCCAGGAGG 
     3961 AAATGGACGT GGCCCTCATG ATTCCAGGTA ACTGCTCAAT GGAGCGGGGC TCCTGGGCTC 
     4021 ATCCGGAGGG GCCCGTCGCA GGCTCCTGTG TGTCCCAGTA TGCCAACTGG CTGGTGGTGT 
     4081 TGCTCCTTAT CGTCTTCTTG CTGGTGGCCA ATATCCTGTT GCTCAACCTG CTCATCGCCA 
     4141 TGTTCAGCTA CACCTTCAGC AAAGTGCACG GCAACAGCGA CCTCTACTGG AAGGCACAGC 
     4201 GCTACAGCCT CATCCGGGAA TTCCATTCGA GGCCTGCCCT GGCCCCACCC CTCATCATCA 
     4261 TCTCTCATGT GCGCCTCCTC ATCAAGTGGC TGCGCAGGTG TCGCAGGTGT CGCAGGGCTA 
     4321 ACTTGCCCGC CTCCCCAGTC TTCGAGCACT TCCGTGTCTG TCTCTCTAAG GAAGCAGAGC 
     4381 GGAAGCTGCT GACTTGGGAG TCTGTGCACA AAGAGAACTT CCTGTTGGCA CAAGCTCGGG 
     4441 ACAAGCGAGA CAGTGACTCG GAGCGTTTGA AACGCACGTC TCAGAAGGTG GACACTGCAC 
     4501 TGAAGCAGCT GGGACAGATC AGAGAGTACG ATCGGCGTCT GAGGGGGCTG GAGAGAGAGG 
     4561 TCCAACACTG TTCTCGAGTC CTGACTTGGA TGGCTGAGGC CCTTAGCCAC TCTGCCTTGC 
     4621 TGCCTCCAGG GGCCCCACCC CCTCCGAGCC CCACTGGTTC CAAAGACACG CGTACGCGGC 
     4681 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4741 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4801 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4861 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4921 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4981 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5041 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5101 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5161 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5221 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5281 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5341 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5401 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5461 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5521 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5581 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5641 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5701 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5761 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5821 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5881 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5941 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6001 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6061 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6121 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6181 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6241 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6301 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6361 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6421 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6481 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6541 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6601 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6661 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6721 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6781 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6841 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6901 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6961 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7021 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7081 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7141 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7201 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7261 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7321 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7381 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7441 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7501 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7561 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7621 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7681 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7741 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7801 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7861 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7921 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7981 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8041 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8101 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8161 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8221 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8281 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8341 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8401 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8461 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//