LOCUS       MR211796                8481 bp    DNA     circular  SYN 
DEFINITION  Pan2 (Myc-DDK-tagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2)
ACCESSION   NM_133992
VERSION     NM_133992.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Pan2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTTGAG GGTCTGGATC CTGGACTGGC CGAATTTTCC CCAGCTATGC 
     1081 ATTCTACCCT GGACCCAGTC CTGGATGCCC ACCTGAATCC AAGTTTGCTA CAGAATGTGG 
     1141 AGCTAGACCC AGAGGGAGTG GCCTTGGAGG CTCTTCCTGT CCAGGAGTCA GTGCACATAA 
     1201 TGGAAGGTGT CTACTCTGAG TTGCACAGCG TGGTGGCTGA AGTGGGAGTG CCTGTGTCTG 
     1261 TCTCCCACTT TGATTTACAT GAGGAGATGC TGTGGGTGGG GAGCCATGGG GGCCATGCCA 
     1321 CCTCCTTCTT CGGCCCAGCT CTGGAGCGCT ACTCATCCTT TCAGGTCAAT GGTGGTGACG 
     1381 ACATTCGGCA AATCCAGAGC CTGGAGAACG GTATCCTCTT CCTCACCAAG AACAACCTCA 
     1441 AGTACATGGC CCGTGGAGGG CTCATTATAT TTGACTATTT GCTGGATGAA AACGAGGATA 
     1501 TGCACAGCGT CCTCCTGACA GACAACAGCA CTCTGCTCGT TGGGGGGCTG CAGAACCACG 
     1561 TACTGGAGAT TGACCTGAAC ACTGTCCAGG AGACTCAGAA GTATGCAGTC GAGACACCCG 
     1621 GAGTCACCAT CATGAGACAG ACAAATCGTT TCTTCTTCTG TGGCCACACA TCTGGCAAGG 
     1681 TTTCCCTGCG AGACCTCCGT AGTTTTAAAG TGGAGCATGA ATTTGATGCC TTCTCAGGGA 
     1741 GTCTGTCAGA TTTTGATGTT CATGGCAACC TGCTGGCTGC CTGCGGCTTC TCCAGTCGCC 
     1801 TTACCGGCCT GGCCTGTGAC CGTTTCCTCA AAGTGTACGA TCTGCGCATG ATGCGTGCCA 
     1861 TCACACCTCT TCAAGTGCAC GTGGATCCGG CCTTCTTACG ATTCATCCCC ACATACACTT 
     1921 CCCGCCTTGC TATCATCTCC CAGTCAGGTC AATGCCAGTT TTGTGAACCC ACAGGCCTGG 
     1981 CCAACCCAGC CGACATTTTC CATGTGAATC CCGTGGGACC TTTGCTAATG ACGTTTGATG 
     2041 TGTCAGCCAG CAAGCAGGCC CTGGCCTTTG GGGATTCTGA GGGCTGCGTG CATCTCTGGA 
     2101 CTGATTCCCC TGAGCCGTCC TTCAACCCCT ACTCCCGAGA GACTGAGTTT GCCCTGCCCT 
     2161 GTCTTGTGGA CTCCTTGCCG CCTCTGGACT GGAGCCAGGA TCTGCTGCCG CTTTCCCTCA 
     2221 TTCCCGTCCC ACTTACCACT GATGCACTGC TCTCTGACTG GCCTGCTGCC AACTCTGCTC 
     2281 CCGCTCCCAG GCGAGCACCA CCTGTGGATG CAGAAATTCT TCGAACCATG AAGAAAGTGG 
     2341 GCTTCATTGG CTATGCTCCC AACCCTCGCA CCAGGCTGCG CAACCAGATT CCCTATCGAC 
     2401 TAAAGGAGTC AGACCATGAA TTTGACAACT TCAGCCAAGT CACGGAGTCA CCGACAGGGC 
     2461 GAGAAGAGGA GCCTCTCCAC ACAGTTTCTA AGAAATACCG GAAGGTAACC ATCAAATATT 
     2521 CCAAGCTAGG CCTGGAAGAC TTTGACTTCA AACACTACAA TAAGACTCTG TTTGCTGGGT 
     2581 TAGAGCCTCA CATCCCCAAT GCCTACTGTA ACTGCATGAT CCAGGTGCTC TATTTCTTGG 
     2641 AGCCTGTTCG CTGTCTGATC CAGAACCACC TTTGCCAAAA GGAGTTCTGC CTAGCGTGCG 
     2701 AGCTCGGCTT CCTCTTCCAC ATGCTAGACC TCTCTCGTGG CGATCCTTGT CAGGGCAGTA 
     2761 ATTTCCTCCG AGCTTTCCGG ACCATCCCTG AGGCCTCAGC TCTGGGTCTC ATCCTTGCTG 
     2821 ACTCAGATGA GGCTTCAGGC AAGGGCAGTC TGGCCCGGCT CATCCAGAGG TGGAACCGCT 
     2881 TCATCCTCAC TCAGCTACAT CAGGATATGC AGGAGCTGGA AGTGCCCCAG GCTTATCGAG 
     2941 GTGCTGGAGG CAGTTTCTGC TCATCAGGAG ACTCCATCAT TGGGCAGCTG TTCAGTTGTG 
     3001 AGATGGAGAA CTGCAGTCTT TGCCGCTGCG GCAGCGAGAC TGTGCGGGCC TCGTCCACCC 
     3061 TACTCTTCAC ACTCTCCTAC CCTGAGGATA AAACCGGGAA GAACTATGAC TTTGCTCAGG 
     3121 TGCTGAAGCG AAGTATCTGC CTGGAGCAGA ACACACAGGC CTGGTGTGAC AACTGTGAGA 
     3181 AGTACCAGCC CACAATTCAG ACCAGAAACA TCCGACATCT GCCAGATATT CTTGTCATTA 
     3241 ACTGTGAAGT GAATAGCTCA AAAGAGGCTG ATTTCTGGAG ACTGCAAGCT GAGGTTGCCT 
     3301 TCAAAATAGC GGTGAAAAAG TACGGCGGGG AAATGAAGAG CAAAGAGTTT GCTTTAGCTG 
     3361 ATCGGAAGGA ACTGAGGAGT CCAGAGGGCT TTCTGTGTTC CTCCATCGAG GAGCTGAAGA 
     3421 ATGTCTGGCT TCCATTTTCC ATCCGCATGA AGATGACCAA GAACAAAGGA CTGGATGTTT 
     3481 GCAACTGGGC TGACGAGCAT GAGCTTAGCA GCCTGGGTGC CCCCTCACAG TGGGGTCCGG 
     3541 CCAGGGCAGA GGAGGAGCTT GGTGTCTATG TATATGACCT GATGGCTACT GTGGTACACA 
     3601 TCCTGGACTC ACGAACAGGC GGCAGCTTGG TGGCTCACAT CAAAGTCGGT GAGACCTACC 
     3661 ACCAGCGCAA GGAGGGCGTG ACTCATCAGC AGTGGTATCT CTTCAATGAC TTCCTTATTG 
     3721 AACCTATTGA TAAGTATGAA GCCGTGCAGT TTGACATGAA TTGGAAAGTA CCTGCTATCC 
     3781 TGTATTATGT CAAAAGGAAT CTCAATTCCA GATACAATCT GAATATCAAG AACCCCATTG 
     3841 AGGCCAGTGT GCTGCTGGCA GAAGCCTCGC TGGCACGGAA GCAACGGAAA ACACACACGA 
     3901 CCTTTATTCC ATTGATGCTG AATGAGATGC CACAGGTCGG GGACCTGGTG GGCCTGGATG 
     3961 CCGAGTTTGT CACTCTTAAT GAGGAAGAAG CAGAGTTACG CAGTGATGGT ACCAAGTCCA 
     4021 CCATTAAGCC AAGCCAGATG TCAGTAGCAA GGATCACCTG TGTTCGGGGC CAAGGGCCTA 
     4081 ACGAGGGCAT CCCCTTCATT GATGACTACA TCTCCACGCA GGAGCAGGTA GTAGATTACT 
     4141 TGACTCAGTA CTCGGGGATA AAGCCAGGAG ACCTTGATGC CAAAATCTCC TCAAAGCACC 
     4201 TCACAACCCT CAAGTCTACC TACTTAAAGC TTCGCTTTCT CATTGATATT GGAGTCAAGT 
     4261 TTGTGGGTCA TGGTCTGCAG AAGGACTTCC GGGTCATCAA CCTCATGGTG CCCAAGGACC 
     4321 AAGTTCTTGA CACAGTCTAC CTGTTCCACA TGCCCCGAAA ACGAATGATT TCCCTACGAT 
     4381 TCCTGGCCTG GTATTTTCTA GACCTGAAGA TTCAAGGTGA GACCCATGAC AGCATTGAGG 
     4441 ATGCCCGCAC AGCCCTTCAA CTCTACCGGA AGTATCTGGA GCTGAGCAAG AACGGCACCG 
     4501 AGCCCGAGTC CTTCCACAAG GTGCTCAAGG GTCTCTATGA GAAGGGCCGA AAGATGGACT 
     4561 GGAAGGTGCC TGAGCCTGAA AGCCAGACAA GTCCCAAGAA TGCAGCTGTC TTCTCAGTGC 
     4621 TGGCACTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//