LOCUS       MR211795                8481 bp    DNA     circular  SYN 
DEFINITION  Egf (Myc-DDK-tagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592)
ACCESSION   BC017681
VERSION     BC017681.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Egf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTGGGGC CGAAGGCCAA CCTGGCTGTT GCTCGCCTTC CTGCTGGTGT 
     1081 TTTTAAAGAT TAGCATACTC AGCGAGAGAA GCGGGACTTG TGCCGGTCCT GCCCCCTTCC 
     1141 TAGTTTTCTC ACAAGGAAAG AGCATCTCTC GGATTGACCC AGATGGAACA AATCACCAGC 
     1201 AATTGGTGGT GGATGCTGGC ATCTCAGCAG ACATGGATAT TCATTATAAA AAAGAGAGAC 
     1261 TCTATTGGGT GGATGTAGAA AGACAAGTTT TGCTAAGAGT TTTCCTTAAC GGGACAGGAC 
     1321 TAGAGAAAGT GTGCAATGTA GAGAGGAAGG TGTCTGGGCT GGCCATAGAC TGGATAGATG 
     1381 ATGAAGTTCT CTGGGTAGAC CAACAGAACG GAGTCATCAC CGTAACAGAT ATGACAGGGA 
     1441 AAAATTCCCG AGTTCTTCTA AGTTCCTTAA AACATCCGTC AAATATAGCA GTGGATCCAA 
     1501 TAGAGAGGTT GATGTTTTGG TCTTCAGAGG TGACCGGCAG CCTTCACAGA GCACACCTCA 
     1561 AAGGTGTTGA TGTAAAAACA CTGCTGGAGA CAGGGGGAAT ATCGGTGCTG ACTCTGGATG 
     1621 TCCTGGACAA ACGGCTCTTC TGGGTTCAGG ACAGTGGCGA AGGAAGCCAC GCTTACATTC 
     1681 ATTCCTGTGA TTATGAGGGT GGCTCCGTCC GTCTTATCAG GCATCAAGCA CGGCACAGTT 
     1741 TGTCTTCAAT GGCCTTTTTT GGTGATCGGA TCTTCTACTC AGTGTTGAAA AGCAAGGCGA 
     1801 TTTGGATAGC CAACAAACAC ACGGGGAAGG ACACGGTCAG GATTAACCTC CATCCATCCT 
     1861 TTGTGACACC TGGAAAACTG ATGGTAGTAC ACCCTCGTGC ACAGCCCAGG ACAGAGGACG 
     1921 CTGCTAAGGA TCCTGACCCC GAACTTCTCA AACAGAGGGG AAGACCATGC CGCTTCGGTC 
     1981 TCTGTGAGCG AGACCCCAAG TCCCACTCGA GCGCATGCGC TGAGGGCTAC ACGTTAAGCC 
     2041 GAGACCGGAA GTACTGCGAA GATGTCAATG AATGTGCCAC TCAGAATCAC GGCTGTACTC 
     2101 TTGGGTGTGA AAACACCCCT GGATCCTATC ACTGCACATG CCCCACAGGA TTTGTTCTGC 
     2161 TTCCTGATGG GAAACAATGT CACGAACTTG TTTCCTGCCC AGGCAACGTA TCAAAGTGCA 
     2221 GTCATGGCTG TGTCCTGACA TCAGATGGTC CCCGGTGCAT CTGTCCTGCA GGTTCAGTGC 
     2281 TTGGGAGAGA TGGGAAGACT TGCACTGGTT GTTCATCGCC TGACAATGGT GGATGCAGCC 
     2341 AGATCTGTCT TCCTCTCAGG CCAGGATCCT GGGAATGTGA TTGCTTTCCT GGGTATGACC 
     2401 TACAGTCAGA CCGAAAGAGC TGTGCAGCTT CAGGACCACA GCCACTTTTA CTGTTTGCAA 
     2461 ATTCCCAGGA CATCCGACAC ATGCATTTTG ATGGAACAGA CTACAAAGTT CTGCTCAGCC 
     2521 GGCAGATGGG AATGGTTTTT GCCTTGGATT ATGACCCTGT GGAAAGCAAG ATATATTTTG 
     2581 CACAGACAGC CCTGAAGTGG ATAGAGAGGG CTAATATGGA TGGGTCCCAG CGAGAAAGAC 
     2641 TGATCACAGA AGGAGTAGAT ACGCTTGAAG GTCTTGCCCT GGACTGGATT GGCCGGAGAA 
     2701 TCTACTGGAC AGACAGTGGG AAGTCTGTTG TTGGAGGGAG CGATCTGAGC GGGAAGCATC 
     2761 ATCGAATAAT CATCCAGGAG AGAATCTCGA GGCCGCGAGG AATAGCTGTG CATCCAAGGG 
     2821 CCAGGAGACT GTTCTGGACG GACGTAGGGA TGTCTCCACG GATTGAAAGC GCTTCCCTTC 
     2881 AAGGTTCCGA CCGGGTGCTG ATAGCCAGCT CCAATCTACT GGAACCCAGT GGAATCACGA 
     2941 TTGACTACTT AACAGACACT TTGTACTGGT GTGACACCAA GAGGTCTGTG ATTGAAATGG 
     3001 CCAATCTGGA TGGCTCCAAA CGCCGAAGAC TTATCCAGAA CGACGTAGGT CACCCCTTCT 
     3061 CTCTAGCCGT GTTTGAGGAT CACCTGTGGG TCTCGGATTG GGCTATCCCA TCGGTAATAA 
     3121 GGGTGAACAA GAGGACTGGC CAAAACAGGG TACGTCTTCA AGGCAGCATG CTGAAGCCCT 
     3181 CGTCACTGGT TGTGGTCCAT CCATTGGCAA AACCAGGTGC AGATCCCTGC TTATACAGGA 
     3241 ATGGAGGCTG TGAACACATC TGCCAAGAGA GCCTGGGCAC AGCTCGGTGT TTGTGTCGTG 
     3301 AAGGTTTTGT GAAGGCCTGG GATGGGAAAA TGTGTCTCCC TCAGGATTAT CCAATCCTGT 
     3361 CAGGTGAAAA TGCTGATCTT AGTAAAGAGG TGACATCACT GAGCAACTCC ACTCAGGCTG 
     3421 AAGTACCAGA CGATGATGGG ACAGAATCTT CCACACTAGT GGCTGAAATC ATGGTGTCAG 
     3481 GCATGAACTA TGAAGATGAC TGTGGTCCCG GGGGGTGTGG AAGCCATGCT CGATGCGTTT 
     3541 CAGACGGAGA GACTGCTGAG TGTCAGTGTC TGAAAGGGTT TGCCAGGGAT GGAAACCTGT 
     3601 GTTCTGATAT AGATGAGTGT GTGCTGGCTA GATCGGACTG CCCCAGCACC TCGTCCAGGT 
     3661 GCATCAACAC TGAAGGTGGC TACGTCTGCA GATGCTCAGA AGGCTACGAA GGAGACGGGA 
     3721 TCTCCTGTTT CGATATTGAC GAGTGCCAGC GGGGGGCGCA CAACTGCGCT GAGAATGCCG 
     3781 CCTGCACCAA CACGGAGGGA GGCTACAACT GCACCTGCGC AGGCCGCCCA TCCTCGCCCG 
     3841 GACGGAGTTG CCCTGACTCT ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG 
     3901 ACCGAAATAG TTATCCAGGA TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG 
     3961 TGTGCATGCA TATTGAATCA CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG 
     4021 GGGATCGATG TCAGACTCGA GACCTACGAT GGTGGGAGCT GCGTCATGCT GGCTACGGGC 
     4081 AGAAGCATGA CATCATGGTG GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCCTCT 
     4141 TGGGGATGTG GGGGACTTAC TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA 
     4201 ACCCTTGTGA TGAGCCAAGC GGAAGTGTGA GCAGCAGCGG GCCCGACAGC AGCAGCGGGG 
     4261 CAGCTGTGGC TTCTTGTCCC CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA 
     4321 AGAATGGGAG TCTGCCTGCG GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC 
     4381 CCTCCCTGCA GCTCGGGTCA GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG 
     4441 GAATGGGCAC AGGGCAAAGC TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA 
     4501 TAGAGGGAAA CTCCCACCTA CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC 
     4561 TCCAGTCAGC TAATGGATCG TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC 
     4621 CAGTTCAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//