LOCUS       MR211775                8442 bp    DNA     circular  SYN 
DEFINITION  Nup98 (Myc-DDK-tagged) - Mouse nucleoporin 98 (Nup98)
ACCESSION   NM_022979
VERSION     NM_022979.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4599..4690 
                     /label="Myc-DDK"  
     misc_feature    4720..5305 
                     /label="PolyA signal"  
     misc_feature    4777..4796 
                     /label="Restriction Enzyme"  
     misc_feature    5581..6253 
                     /label="ColE1"  
     misc_feature    6683..7477 
                     /label="Kan/Neo"  
     misc_feature    7512..7814 
                     /label="SV40 ori"  
     misc_feature    7900..8377 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4581..4587 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4581 
                     /label="Nup98"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTAACAAA TCATTTGGAA CCCCCTTTGG GGGTAGTACA GGGGGCTTTG 
     1081 GCACAACGTC AACATTTGGG CAAAATACTG GCTTTGGTAC GACTAGTGGA GGAGCATTTG 
     1141 GAACATCTGC ATTTGGTTCT AGCAACAATA CTGGAGGCTT ATTTGGAAAT TCACAGACCA 
     1201 AACCAGGAGG ATTATTTGGT ACCAGTTCAT TTAGCCAGCC AGCAACCTCC ACAAGCACTG 
     1261 GGTTTGGGTT TGGCACATCA ACAGGAACAT CAAATAGCTT ATTTGGAACT GCAAGTACCG 
     1321 GGACCAGTCT TTTCTCATCC CAGAACAATG CATTTGCACA AAATAAACCA ACTGGCTTTG 
     1381 GGAATTTTGG AACCAGTACT AGCAGTGGAG GACTCTTTGG AACTACAAAT ACCACCTCTA 
     1441 ATCCTTTTGG TAGCACATCT GGCTCCCTTT TTGGGCCAAG TAGTTTTACA GCAGCACCTA 
     1501 CAGGAACTAC CATCAAATTT AATCCTCCCA CTGGTACAGA TACTATGGTC AAAGCTGGAG 
     1561 TTAGCACTAA CATCAGTACA AAGCATCAGT GTATTACTGC TATGAAAGAA TATGAAAGCA 
     1621 AGTCATTAGA GGAACTACGT TTGGAGGATT ATCAGGCTAA CCGGAAAGGC CCACAGAACC 
     1681 AAGTGGGAGG AGGCACCACG GCTGGCTTAT TTGGGTCTTC TCCAGCAACT TCCAGTGCAA 
     1741 CAGGGCTCTT CAGCTCCTCC ACCACTAATT CAGCCTTTTC ATATGGTCAG AACAAAACTG 
     1801 CCTTTGGAAC TAGCACAACT GGATTTGGAA CAAATCCAGG TGGTCTCTTT GGCCAACAGA 
     1861 ATCAACAGAC TACCAGTCTC TTCAGCAAAC CATTTGGCCA GGCTACAACC ACCCCGAATA 
     1921 CTGGCTTTTC CTTTGGTAAT ACCAGCACCC TTGGACAGCC AAGCACCAAT ACTATGGGCC 
     1981 TATTTGGAGT AACCCAAGCC TCACAACCAG GAGGTCTTTT TGGGACAGCT ACAAACACCA 
     2041 GCACTGGGAC AGCATTTGGG ACAGGAACAG GTCTCTTTGG GCAGCCCAAT ACTGGATTTG 
     2101 GTGCAGTTGG TTCGACCCTG TTTGGCAATA ACAAGCTTAC AACTTTTGGA ACCAGCACAA 
     2161 CCAGTGCTCC TTCATTTGGT ACAACCAGTG GCGGGCTCTT CGGTAACAAA CCAACCCTGA 
     2221 CTTTAGGAAC CAATACAAAC ACTTCCAATT TTGGGTTTGG CACAAATAAC AGTGGGAGCA 
     2281 GTATTTTTGG AAGTAAGCCA GCAGCTGGAA CTTTGGGAAC TGGACTTGGT ACAGGATTTG 
     2341 GAACAGCTCT TGGTGCTGGA CAGGCATCTT TGTTTGGAAA CAACCAACCT AAGATTGGAG 
     2401 GGCCTCTTGG TACAGGAGCC TTTGGGGCCC CTGGATTTAA TACTTCGACA GCCATTTTGG 
     2461 GCTTTGGCGC CCCCCAGGCC CCAGTAGCTT TGACAGATCC AAATGCTTCT GCTGCCCAGC 
     2521 AGGCTGTTCT CCAGCAGCAC CTCAATAGCC TAACATACTC ACCCTTTGGA GACTCCCCCC 
     2581 TCTTCCGGAA TCCTATGTCA GATCCTAAGA AGAAAGAAGA GAGACTGAAA CCAACCAATC 
     2641 CAGCTGCTCA GAAAGCTCTT ACAACACCTA CTCATTATAA ACTTACACCT CGCCCTGCTA 
     2701 CCAGAGTCAG GCCAAAGGCT TTGCAAACAA CAGGCACAGC CAAATCACAT CTCTTTGATG 
     2761 GGCTGGATGA CGATGAACCA TCTCTAGCCA ACGGAGCATT CATGCCTAAA AAGAGCATCA 
     2821 AGAAGTTGGT TTTGAAAAAT CTCAACAATA GCAATCTCTT TTCTCCTGTT AATCATGATT 
     2881 CAGAAGATCT AGCTTCACCC TCTGAGTATC CAGAAAATGG AGAAAGATTT AGCTTCCTGA 
     2941 GCAAACCTGT TGATGAGAAC AATCAGCAGG ATGGAGAAGA TGACTCTCTT GTATCACGAT 
     3001 TTTACACTAA TCCTATTGCC AAACCCATTC CACAAACTCC AGAGAGTGTT GGAAACAAAA 
     3061 ATAACAGTAG CAGCAATGTG GAAGATACCA TTGTTGCCTT GAACATGCGT GCTGCTTTGC 
     3121 GCAATGGATT GGAAGGAAGC AGTGAAGAGA CGTCATTCCA TGATGAGTCA TTGCAAGATG 
     3181 ACCGAGAAGA GATAGAAAAT AATGCTTACC ACATACACCC AGCAGGCATT GTTCTCACAA 
     3241 AAGTTGGTTA TTACACTATC CCATCTATGG ATGACCTTGC TAAAATTACC AACGAGAAGG 
     3301 GAGAATGCAT TGTTTCTGAC TTCACCATTG GTCGTAAAGG ATATGGCTCA ATCTATTTTG 
     3361 AAGGAGATGT GAATTTGACA AATCTAAATT TGGATGATAT TGTGCATATC CGAAGGAAAG 
     3421 AAGTTATTGT CTATGTAGAT GATAACCAAA AGCCACCTGT GGGTGAAGGG CTAAATAGGA 
     3481 AGGCTGAAGT TACTTTGGAT GGAGTTTGGC CAACAGATAA AACATCCCGG TGTTTAATAA 
     3541 AGAGTCCAGA TCGACTTGCT GATATCAACT ATGAGGGGAG ATTAGAAGCA GTCTCAAGAA 
     3601 AGCAAGGGGC CCAATTCAAG GAGTATCGGC CTGAAACTGG TTCTTGGGTA TTTAAGGTCT 
     3661 CCCATTTTTC CAAGTATGGC CTTCAGGATT CTGATGAAGA GGAGGAGGAA CACCCACCCA 
     3721 AAACGACTTC AAAGAAGCTG AAGACTGCCC CTTTGCCCCC TGCAGGCCAG GCAACCACTT 
     3781 TCCAGATGAC TCTTAATGGC AAGCCTGCAC CCCCACCTCA GAGCCAGAGC CCAGAAGTGG 
     3841 AGCAGTTAGG CAGGGTTGTG GAACTGGACA GCGACATGGT AGATATCACC CAGGAGCCAG 
     3901 TTCCAGATTC TGTGTTAGAA GAGAGTGTGC CCGAGGATCA GGAGCCTGTG TCTGCTTCAA 
     3961 CGCATATTGC ATCTTCACTG GGAATTAATC CACATGTTTT ACAGATCATG AAAGCATCAT 
     4021 TGCTTGTTGA CGAAGAAGAT GTAGATGCCA TGGATCAACG CTTTGGTCAC ATCCCTTCCA 
     4081 AAGGAGAGAC TGTCCAGGAA ATCTGTTCTC CTAGACTCCC CATTTCAGCC TCCCACTCAT 
     4141 CAAAATCCCG CTCCATAGTT GGTGGGTTGC TGCAATCAAA ATTTGCAAGT GGAACTTTTC 
     4201 TTTCACCAAG TGCCTCCGTG CAAGAATGTC GCACTCCCAG GACATCATCT CGCATGAACA 
     4261 TCCCATCCAC ATCCCCCTGG TCTGTCCCTC TGCCCCTGGC CACTGTGTTC ACAGTGCCCA 
     4321 GCCCAGCCCC TGAGGTTCAG CTAAAAACAG TGGGGATACG AAGGCAACCA GGCCTAGTCC 
     4381 CTCTTGAAAA ATCCATTACA TATGGCAAGG GGAAGCTCTT GATGGACATG GCCCTATTCA 
     4441 TGGGACGTTC ATTTCGGGTT GGTTGGGGTC CCAACTGGAC TCTTGCTAAT AGTGGAGAAC 
     4501 AACTGCATGG CTCCCATGAA CTGGAAAATC ATCAGGTTGC CGATTCTATG GAATATGGAT 
     4561 TCCTGCCCAA TCCAGTAGCT GTTAAATCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4621 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4681 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4741 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4801 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4861 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4921 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4981 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5041 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5101 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5161 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5221 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5281 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5341 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5401 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5461 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5521 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5581 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5641 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5701 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5761 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5821 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5881 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5941 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6001 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6061 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6121 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6181 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6241 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6301 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6361 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6421 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6481 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6541 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6601 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6661 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6721 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6781 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6841 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6901 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6961 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7021 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7081 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7141 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7201 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7261 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7321 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7381 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7441 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7501 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7561 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7621 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7681 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7861 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7921 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7981 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8041 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8101 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8161 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8221 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8281 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8341 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8401 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//