LOCUS       MR211767                8427 bp    DNA     circular  SYN 
DEFINITION  Phlpp (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase (cDNA clone MGC:67583 IMAGE:6406203)
ACCESSION   BC059254
VERSION     BC059254.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="Phlpp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTTGCCA GTGAACCGAT GGACAAGACG ACAGGTCATT CTGTGCGGGA 
     1081 CGTGCTTGAT AGTGTCGTCC GTGAAAGACA GCGTGAGTGG GAAGATGCAT GTCCTGCCCC 
     1141 TCATTGGTGG AAAAGTGGAG GAAGTGAAAA AGCACCAGCA CTGTTTAGCG TTCAGCTCTT 
     1201 CGGGGCCTCA AAGCCAGACT TACTACATCT GCTTTGATAC TTTCACGGAG TACTTACGGT 
     1261 GGCTGCGGCA AGTCTCCAAG GTTGCATCAC AGCGTATAAG CTCAGTAGAC CTCTCCTGTT 
     1321 GTAGCCTTGA ACATCTGCCT GCCAACCTCT TTTACAGCCA AGACCTTACT CATCTCAACT 
     1381 TAAAACAGAA CTTCCTAAGG CAAACCCCCA CCCTCCCAGC TGCCAGAGGA CTTGGTGAAC 
     1441 TGCAAAGGTT CACCAAATTG AAGAGCCTTA ACCTTTCCAA TAACCACCTA GGAGCCTTTC 
     1501 CGTCAGCAGT CTGCAGCATT CCAACCCTAG CAGAGCTGAA TGTATCTTGC AATGCCCTGC 
     1561 GAGAAGTCCC AGCAGCTGTT GGAGATATGC AGAACTTGCA GACGTTTCTG CTGGATGGAA 
     1621 ATTTTCTCCA GTCCCTTCCT GCTGAACTGG AGAGCATGCA CCAGCTCAGC TATCTGGGTC 
     1681 TTTTCTTTAA TGAATTCACT GACATTCCAG AGGTATTGGA GAAGCTGACT GCTGTGGATA 
     1741 AGCTGTGTAT GGCTGGGAAC TGTGTGGAGA CCCTCAGACT ACAGGCCTTA AGAAGAATGC 
     1801 CTCATATTAA ACATGTGGAC CTAAGACTGA ACATACTCAG AAAGCTTATG GCAGATGAGG 
     1861 TGGACTTTGT GCAACATGTC ACTCAGCTTG ACCTGCGAGA CAATAAGCTT GGTGATCTAG 
     1921 ATGCTATGAT CTTCAACAAC ATAGAAGTTC TGCACTGTGA GAGGAATCAG CTGGTGACAT 
     1981 TGAACGTTTG TGGCTATTTC CTAAAGGCAC TCTATGCTTC TTCTAATGAA CTTGCTCAAC 
     2041 TTGATGTCTA CCCAGTTCCG AATTATCTCT CTTACATGGA TGTCTCAAGA AACTGCCTAG 
     2101 AAAGTGTGCC TGAGTGGGTA TGTGAAAGCC GGAAATTAGA AGTTTTGGAT ATTGGCCATA 
     2161 ATCAAATATG TGAACTTCCT GCCCGCCTGT TTTGTAATAG TAGTCTCCGG AAATTGCTAG 
     2221 CAGGACACAA CCGGTTGGCA AGGCTTCCTG AAAGGCTGGA GAGAACTTCT GTGGAGGTCT 
     2281 TGGATGTCCA GCACAACCAG ATCACTGAGC TCCCACCAAA CCTCCTCATG AAGGCTGACA 
     2341 GCCTGAGATT CCTGAATGCA TCTGCAAACA AACTGGAAAC CCTGCCTCCA GCCACACTTT 
     2401 CTGAGGAGAC GAGCAGTATA TTACAGGAGT TGTACCTGAC AAACAACTGC CTCACGGATA 
     2461 AGTGTGTGCC CTTGTTAACA GGGCACCCCC GTCTGAAGAT CCTACACATG GCCTATAACC 
     2521 GGCTTCAGAG CTTCCCGGCA AGTAAAATGG CAAAACTGGA GGAACTTGAA GAAATTGATA 
     2581 TCAGTGGGAA TAAGCTGAAA GCCATCCCCA CAACAATCAT GAACTGCAGA CGCATGCACA 
     2641 CCGTGATTGC TCACTCCAAT TGTATCGAAG TCTTTCCTGA AGTTATGCAG CTCCCAGAAG 
     2701 TCAAGTGTGT AGATCTGAGC TGTAATGAGC TGAGTGAAAT CACGTTACCA GAAAACCTGC 
     2761 CACCTAAGCT GCAGGAGCTA GACCTGACTG GAAATCCACG CCTTGCCCTG GACCACAAAA 
     2821 GCCTGGAGCT GCTCAATAAC ATTCGCTGCT TCAAGATTGA CCAGCCGTCA GCAGGAGATG 
     2881 CATCAGGAGC CCCAGCAGTA TGGAGTCATG GTTACACTGA AGCATCGGGA GTAAAAAACA 
     2941 AGCTGTGTGT CGCAGCCCTG TCTGTAAATA ACTTCCGTGA CAACCGAGAG GCCCTCTATG 
     3001 GTGTGTTTGA TGGAGACCGG AATGTGGAGG TGCCCTACCT TCTCCAGTGC ACCATGAGTG 
     3061 ACATTTTGGC TGAAGAGCTT CAGAAAACGA AAAATGAAGA AGAATACATG GTCAATACAT 
     3121 TCATAGTCAT GCAAAGGAAA CTGGGAACTG CTGGGCAGAA ACTTGGTGGT GCTGCTGTCC 
     3181 TCTGCCACAT CAAGCCTGAC CCTGTGGACC TGGGAGGATC CTTTACCCTG ACCTCTGCTA 
     3241 ATGTTGGCAA GTGCCAAACG GTTCTCTGTC GAAATGGGAA GCCACTGTCT CTGTCCAGGT 
     3301 CATACATCAT GAGCTGTGAA GAAGAGCGGA AGAGGATTAA GCAGCACAAA GCCATCATCA 
     3361 CTGAGGATGG CAAGGTCAAT GGAGTGACAG AGTCCACGCG CATCCTGGGC TACACCTTCC 
     3421 TCCATCCCAG TGTGGTGCCT CGCCCCCATG TGCAGTCGGT GCTTCTGACT CCACAGGATG 
     3481 AGTTTTTCAT CCTGGGCAGC AAAGGGCTGT GGGACAGCCT ATCCATTGAC GAGGCTGTGG 
     3541 AAGCTGTACG CAATGTGCCA GATGCTCTGG CTGCTGCTAA GAAGCTCTGC ACCCTGGCTC 
     3601 AGAGCTATGG CTGCCATGAC AGCATCAGTG CTGTGGTGGT ACAGCTAAGT GTCACCGAAG 
     3661 ACAGCTTCTG CTGCTGTGAG CTCAGTGCTG GAGGGAGCAT GCCACCACCC AGCCCCGGAA 
     3721 TCTTCCCACC ATCCGTCAAC ATGGTCATCA AGGACCGACC CTCAGATGGG CTGGGTGTGC 
     3781 CATCCTCCAG CAGTGGCATG GCATCTGAGA TCAGCAGTGA ACTGTCTACC TCCGAGATGA 
     3841 GTAGTGAGGT GGGCTCCACA GCCTCTGATG AGCCCCCGTC TGGAGTCCTG AATGAGAGCA 
     3901 GCCCTGCCTA CCCCAACGAG CAGCGCTGCA TGCTCCACCC TGTCTGCCTG TCTAACTCCT 
     3961 TCCAACGTCA GCTGTCCAGC GCTACTTTCT CCAGCGCGTT CTCTGACAAC GGCCTTGACA 
     4021 GTGACGATGA AGAACCCATT GAGGGGGTGT TCAGCAACGG CAGCCGGGTT GAGGTGGAAG 
     4081 TAGACATCCA CTGCAGCAGG GCCAAGGAAA AGGAGAGACA GCAGCACCTA CTTCAGGTGC 
     4141 CAGCTGAGGC CAGTGATGAG GGCATTGTCA TCAGTGCCAA TGAGGATGAG TCAGGTCTGT 
     4201 CCAAGAAGGC AGACTTTTCT GCTGTGGGAA CCATTGGACG ACGCAGGGCT AATGGCTCTG 
     4261 TAGCTCCCCA GGAAAGGAGC CATAATGTGA TAGAGGTTGC TGCAGATGCG CCTCTCCGGA 
     4321 AGCCAGGAGG CTATTTTGCA GCCCCTGCTC AGCCAGATCC AGATGATCAG TTTATCATCC 
     4381 CCCCAGAGCT GGAAGAGGAA GTCAAAGAAA TCATGAAACA TCACCAGGAG CAGCAGCAGC 
     4441 AGCAGCAGCA GCAGCAGCTG CCACCACCAC CCCAGCCGCC ACAGCCACAG CCACAGCCAC 
     4501 AGCCACAGCC ACAGCCACAG CCACAGCGGC ACTTCCAAAT GGATCACCTG CCAGACTGTT 
     4561 ACGATACACC GCTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//