LOCUS       MR211761                8415 bp    DNA     circular  SYN 
DEFINITION  Lars (Myc-DDK-tagged) - Mouse leucyl-tRNA synthetase (Lars)
ACCESSION   NM_134137
VERSION     NM_134137.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4572..4663 
                     /label="Myc-DDK"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    4750..4769 
                     /label="Restriction Enzyme"  
     misc_feature    5554..6226 
                     /label="ColE1"  
     misc_feature    6656..7450 
                     /label="Kan/Neo"  
     misc_feature    7485..7787 
                     /label="SV40 ori"  
     misc_feature    7873..8350 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4560 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="Lars"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGAAGA AAAGGAACTG CTAAAGTAGA CTTCTTGAAG GAGATCGAGA 
     1081 AAGAGGCCCA GCAGAAGTGG GAAGCTGAGA AAGTGTTTGA AGTCAGTGCC TCCCGGCTGG 
     1141 AGAAGCAGAA GCAGAGCAGC AAGGGCAAAT ACTTTGTCAC CTTCCCATAT CCATACATGA 
     1201 ATGGACGCCT TCATTTGGGA CACACGTTTT CCTTATCCAA GTGTGAGTTT GCAGTAGGAT 
     1261 ACCAGAGATT AAAAGGAAAA AGCTGTCTGT TTCCCTTTGG GTTGCACTGT ACTGGTATGC 
     1321 CTATTAAGGC ATGCGCTGAC AAGCTGAAAA GAGAAATAGA GCTGTATGGC TGCCCTCCTG 
     1381 ATTTTCCCGA GGAAGAAGAG GAAGAGGAAG AAAGCAGTGC TAAACCTGGA GATATAGTAG 
     1441 TGAGGGATAA AGCCAAAGGG AAAAAGAGCA AAGCTGCCGC CAAAGCAGGA TCTTCTAAGT 
     1501 ACCAGTGGGA CATTATGAAA TCCCTTGGGC TCTCTGATGA CGACATAGTG AAGTTTTCTG 
     1561 AAGCAGAGCA CTGGCTTGAT TATTTCCCAC CACTGGCCGT TCAGGATCTG AAGACTATAG 
     1621 GTTTGAAGGT AGACTGGCGG CGCTCCTTCA TCACCACGGA CGTGAATCCT TACTACGATT 
     1681 CCTTTGTCAG GTGGCAGTTT TTAACTTTAA GAGAAAGAAA TAAAATTAAA TTTGGGAAGC 
     1741 GGTACACTAT TTATTCCCCA AAAGATGGAC AGCCTTGCAT GGATCATGAC AGACAAACTG 
     1801 GAGAGGGTGT TGGACCTCAG GAATATACTC TGGTCAAACT GAAGGTGCTT GAACCATATC 
     1861 CATCAAAATT AAGCGGCCTG AAAGGGAAAA ATATCTTCCT AGTAGCTGCT ACACTCAGAC 
     1921 CTGAGACTAT GTTTGGGCAG ACAAATTGTT GGGTTCGGCC TGATATGAAA TACATTGGGT 
     1981 TTGAGACAGC AAATGGAGAC ATCTTCATCT GTACCCAGAG AGCAGCCAGG AATATGTCAT 
     2041 ACCAAGGCTT CACCAAGCAC AACGGAGTGG TGCCGGTTGT GAAGGAGTTA ATGGGAGAGG 
     2101 AAATCCTTGG GGCTTCACTT TCTGCACCCC TGACGTGCTA TAAGGTGGTC TACGTTCTCC 
     2161 CTATGCTGAC CATTAAAGAG GATAAAGGCA CTGGGGTGGT CACGAGCGTT CCTTCTGATT 
     2221 CCCCGGATGA TCTTGCAGCA CTCAGAGACT TGAAGAAAAA GCAAGCCTTA CGAACAAAGT 
     2281 TTGGAATTAG GGATGACATG GTCCTGCCGT TTGAACCGGT GCCAGTCCTG GAAATCCCAG 
     2341 GGATTGGAAA TCTTCCTGCT GTGACTGTTT GTGATGAGCT GAAAATTCAG AGCCAGAATG 
     2401 ACCGTGAAAA ACTTGCAGAA GCAAAGGAGA AATTGTATTT AAGAGGATTT TATGATGGTG 
     2461 TAATGCTAGT GGATGGCTTT AAAGGACAGA AGATCCAACA TGTAAAGAAG ACTATTCAGA 
     2521 AAAACATGAT TGATGCTGGA GATGCGCTTA TCTACATGGA GCCAGAAAAA CAAGTGATGT 
     2581 CGAGGTCTGC AGATGAGTGC GTCGTGGCCC TGTGTGACCA GTGGTACTTG GATTATGGAG 
     2641 ATGAGAACTG GAAGAAACAG ACATTTCAGT GCCTGAAGAA CATGGAAACA TTCTGCGAGG 
     2701 AGAGCAGGAA GAATTTTGAA GCTTCCTTAG ACTGGCTACA AGAACATGCT TGCTCAAGAA 
     2761 CGTACGGTTT AGGCACTCGT CTGCCTTGGG ATGAACAGTG GTTAATCGAA TCTCTCTCGG 
     2821 ATTCCACAAT TTACATGGCG TTCTACACAG TGGCACACCT GCTGCAGGGG GGTGACCTGA 
     2881 ATGGGCAGGC GGAGTCTCCA CTGGGCATCA GACCCCAGCA GATGACAAAG GACGTTTGGG 
     2941 ATTATGTGTT CTTCAAGGAT GCTCCATTTC CTAAGACTCA GATCCCAAAG GAGAAGCTGG 
     3001 ATCAGTTGAA GCAGGAGTTT GAGTTTTGGT ACCCAGTGGA CCTTCGAGCC TCTGGCAAGG 
     3061 ATCTGATTCC AAATCATCTT TCATATTACA TTTACAACCA TGTGGCCATG TGGCCAGAGC 
     3121 AAAGTGACAA GTGGCCCGTG TCTGTCCGAG CAAATGGACA CCTGCTGCTC AACTCGGAGA 
     3181 AGATGTCAAA ATCTACGGGC AACTTCCTCA CTTTGAGCCA AGCTGTTGAC AAATTTTCAG 
     3241 CTGACGGAAT GCGCCTGGCT CTGGCTGATG CTGGTGACAC TGTTGAAGAT GCTAACTTTG 
     3301 TGGAAGCCAT GGCAGACGCA GGCATCCTCC GTCTGTACAC ATGGGTAGAG TGGGTCAAAG 
     3361 AAATGCTCGC CAGCTGCAGC AGCCTCAGAA GTGGACCTGC CGACTCATTC AATGATCGTG 
     3421 TGTTTGCCAG TGAAATGAAT GCAGGAATTA TAAAAACAGA TCAAAACTAT GAAAAGATGA 
     3481 TGTTTAAAGA AGCTTTGAAA ACAGGTTTTT TTGAGTTTCA GGCTGCGAAA GACAAATACC 
     3541 GTGAACTGGC CACGGAGGGC ATGCACAGAG AGCTTGTGTT CCGCTTTATT GAAGTTCAGA 
     3601 CAATCCTTCT GACTCCATTC TGTCCACATT TGTGTGAGCA CATCTGGACA CTGCTTGGAA 
     3661 AGCCTGATTC AATCATGCAT GCCTCATGGC CTGTGGCAGG TCCCGTGGAT GAGTCCTTGA 
     3721 TCCGTTCCTC ACAGTATCTC ATGGAAGTAG CACATGACCT TAGATTACGG CTCAAGAATT 
     3781 ACATGATGCC AGCTAAAGGG AAGAAGACTG ACAAGCAGCC AGCTCAGAGG CCCTCACACT 
     3841 GCACCATCTA TGTGGCAAAG AACTACCCCG TCTGGCAGCA CATTACCCTG ACAACCCTGC 
     3901 GGAGCCACTT TGAGGCCAAC AATGGAAAGT TGCCTGATAA CAAAGTCATT GCTAGTGAAC 
     3961 TAGGCAGTTT GCCAGAGTTG AAGAAATATA TGAAGAAAGT GATGCCATTC GTTGCTATGA 
     4021 TTAAGGAAAA CATGGAGAAG AAAGGGCCGA GAGTCTTGGA TTTAGAGCTG GAGTTTGATG 
     4081 AGCAGGCAGT GCTTATGGAG AATATAGTCT ACCTAACCAA TTCTCTCGAG CTGGAACACA 
     4141 TAGAAGTCAA GTTTGCCTCT GAGGCTGAAG ATAAAGTCAG GGAGGAGTGC TGTCCTGGGA 
     4201 AGCCACTTAA CGTTTTCAGA ACAGAACCTG GTGTGCCAGT TTCCCTAGTA AATCCTCAGC 
     4261 CATCGAGTGG GCACTTCTCC ACCAAAATCG ACATCAGGCA AGGAGATAGC TGTGAGTCCA 
     4321 TAATCAGACG CTTGATGAAA ACGGACCGAG GGATCAAAGA TCTTTCCAAA GTGAAGCTGA 
     4381 TGCGGTTTGA TGATCCTCTG TTGGGGCCTC GGCGAGTTCC GGTCCTGGGG AGAGAGCACA 
     4441 GTGAGAAGAC ACTCATTTCC GAGAACGCCG TTTTCCACGT GGACCTGGTG AGCAAGAAAG 
     4501 TCCACCTCAC CGAGAACGGG CTGCGCACGG ACATCGGCGA CACCATGGTC TACCTGGTTC 
     4561 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4621 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6001 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6061 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6121 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6181 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6241 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6301 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6361 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6421 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6481 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6541 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6601 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6661 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6721 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6781 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6841 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6901 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6961 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7021 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7081 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7141 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7201 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7261 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7321 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7381 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7441 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7501 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7561 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7621 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7981 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8041 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8101 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8161 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8221 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8281 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8341 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8401 TTTAACGCGA ATTTT
//