LOCUS       MR211757                9000 bp    DNA     circular  SYN 
DEFINITION  D8Ertd82e (Myc-DDK-tagged) - Mouse DNA segment, Chr 8, ERATO Doi 82, expressed (D8Ertd82e)
ACCESSION   NM_172911
VERSION     NM_172911.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5157..5248 
                     /label="Myc-DDK"  
     misc_feature    5278..5863 
                     /label="PolyA signal"  
     misc_feature    5335..5354 
                     /label="Restriction Enzyme"  
     misc_feature    6139..6811 
                     /label="ColE1"  
     misc_feature    7241..8035 
                     /label="Kan/Neo"  
     misc_feature    8070..8372 
                     /label="SV40 ori"  
     misc_feature    8458..8935 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5139..5145 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5139 
                     /label="Prag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCGTGC AGCGACTTTG TGGAGCACAT CTGGAAACCC GGGTCTTGCA 
     1081 AGAACTGCTT CTGCTTGCGA AGTGATCATC AGCCAACAGC TGGCCATCCC AAGGCTAGAG 
     1141 CCAACAGCCT ACCCGCTGGA ACTCGCCTGC CTGCCAGGCC TGAGAATTGC CGCCTAGACG 
     1201 ACGAAGGCGT GAATGGCTTG GCCTACTCAA AGCCCACCAT TGCTGTAAAG CCCACCATGA 
     1261 TGACTTCTGA GACCTCCGAC TTGTGGACTG AAGCCAGCCT GAGCGCTGAA GTCCCAAAGG 
     1321 TCAATTGGAG ACGAACCCCA GGCAAGCTCC TGCTCCCGAA ACAGGAAGAT GAGCCAATAG 
     1381 TTTACCTAGG CAGCTTCCGA GGACTGCAGA AGCCTGCAAG CCCTCTTGCC TGCACAGATG 
     1441 GCAACTCTCG TTGTCCCCCA GCCTATACCA TGGTCGGACT GCATAACCTG GAGGCCCGCG 
     1501 TGGATAGGAA CACTGCCTTT CAACCAGTGA GCTTCCAGGA AGAGAAGGCT GGGAGGGAAG 
     1561 AGCTGCCCTC AGCTCACGAG AGCTTTCGCC AGAAGCTGGC TGCCTTTGCA GGGATGACAT 
     1621 CCAGCTGCCC CAAGGGCCCC AGGCCCTGCA CTTCTCCACA GCCCCTGCGG GAATCCCTGC 
     1681 CCTCGGAGGA TGACAGTGAC CAAAGGTGCT CACCCTCAGG AGACAGCGAA GGTGGGGAGT 
     1741 ACTGTTCCAT TCTGGACTGT TGTCCAGAAT CCAAAGATGC TGTGCATAGC ACCGAGGGCT 
     1801 CTGGCAGACG TGGAGGGGAC TGCTCACCCA CGTGCAGGGA GCAGGGACCA CGTACAAGAC 
     1861 CAACAGAAGA GGAAAAACAG GGTCTGAGCT TCCCCAGAGA GTGCTGTGGT CAGGGATCCA 
     1921 CAGCGAACCC GCCCCGCCTG GGCCCCAAGA AGCCATCCCT TAACTCTGAG GCCGCCAGCT 
     1981 CTTCCGATGG TCTGTCCTGT GGCAGCAGCC GCAGCGGGGC CAGCAGCCCC TTCGCTCCCC 
     2041 ACCTTGAGAA CGACTACTGC TCTCTTGTGA AGGAACCGGC CTCAGGGAAG CAGCAAGACC 
     2101 TCAGCGGCCA CTTTCTCACC TCTGGCAAAT GTGTGGGTCA AGCTGCGGAA CTCCAGCCCG 
     2161 CCTCCCTTCT TAGGGACCCT GTGCAGCCTG AACCCATCTA CGCCGAAAGC GCCAAGAGGA 
     2221 AGAAGGCAGC TCCGGGGCCT CCGAGGCCCG AGCCCAAGAA AGAGCAGGTC CCAGCTGGCC 
     2281 ATAGCCAGGG CCAAGTATGG ACAGGTGATA CCTGGATTCA GAAGACACCC CCTAGCTGGA 
     2341 GCCAGGACAG GGAAGGCGCA AACCCTGCTC CTCAGGTGGC AACCACCATC ACTGTCATCG 
     2401 CTGCTCACCC GGAAGAGGAC CACAGGACTA TTTATCTGAG CAGCCCTGAC TCTGCCGTGG 
     2461 GGGTGCAGTG GCCACGTGGG CCTTCGAACC AGGACTTACA AGCCGGTGAA GAGGAGCCTT 
     2521 TGGTTGCGCA GGGGCTAACC TCAAGGGAGA GCCATCCACA TAATGTGACT GAAAACACGG 
     2581 CCAAAGAGAA GCCTGCCATT CCCCCCAAAC TGTCCAAAAG CAGCCCCGGA GGGTCCCCTG 
     2641 TGTCTCCCGC GCCCCCACTG ACTGACCACA GCGACGGGAA CACCGGTGGC AGCAGCGTCG 
     2701 GACCCCAACT TTTGTCTAGA GTCCCTGCTA ACCTAACGTC TTCCTGCCAC ACCAATGGCG 
     2761 TTGCCACTGC TGGTGACTCT GCCAAGTGTC CCCCACCAGC CACCTCCTCT TCAGTCTTGG 
     2821 ACCAGAGGCG GCCAAGATAC CAGACTGGTG CTTGGAGCCG CCAGTGCCGG ATAGAAGAGG 
     2881 AGGAGGAGGT GGGGCAGGAG TTGAGTCAAA GTTGGGGGAG AGAGTTGGAA AACGGTACCG 
     2941 CAGACCATTC AAACTCCTCT ACCTGGCACC GTCTCCATCC CATCGATGGC ACCTCTGGGC 
     3001 AGAACAGCAA AACTAACTCT GGGATGAGCA AATCGGCTTC CTTTGCGTTT GAGTTCCCAA 
     3061 AGGACAGAGG CAGATTGGAG GCATTCTCAC CCCCTCCCCC ACCTCCAAAG TCAAGGCACC 
     3121 TGTTAAAAAT GAACAAGAGC AGTTCTGACT TGGAAAAAGT GAGCCAGAGT TCTGCAGAAA 
     3181 GCCTCAGCCC GTCCTTCAGA GGGGCCCACG TCAGCTTCAC CACGGGCTCC ACAGATAGCC 
     3241 TAGCCTCGGA CTCCAGGCCC TGCAGCGATG GAGGTCCATC ATATGAACCC ACCCACTCAC 
     3301 CCACCATCAG CGGGAAAAAG CTCTTCGCTC CCGTCCCTTT TCCATCAGGC TCCACAGAGG 
     3361 ATGTGTCCCC TGGTGGTGGT CCTGCGCAGC CTCCTCCTCT GCCCCAGAAA AAGATAGTGA 
     3421 GCCGTGCCGC CTCTTCCCCT GATGGCTTCT TCTGGACCCA AGGTTCCCCT AAGCCACGGA 
     3481 CGGCAAGTCC CAAGCTGAAC CTAAGCCATT CAGAAACCAA TGTTTGTGCC CATGACGAGC 
     3541 CTCCTTTTAA CTGTTCCTTG AACTCTGGAA ACCGTTCCCA CCATGTCTTC TCCTCTTCTG 
     3601 AGCCTTTGGG GAAAGCTTTC AAAGGCAATG CTCCCTGGGC CCCAGCTCTG GGGCTGGCGA 
     3661 ACAGCAAAGG CGGCTGTGGG AGCCCCAGTC TCCAGTGCAG AGCGGCCACC TCCACGTCAT 
     3721 CATCCCAGCT CAGCGTGTCC AGCCAGGCCT CCTCCAGCAG CACCCAGCTT CAGCTGCACA 
     3781 GCCTGCTGAG TAGCATCAGC AGCAAGGAGG GCACCTATGC CAAGCTTGGG GGCCTCTACA 
     3841 CACAATCCCT GGCCCGCCTA GTGACCAAGT GTGAAGATCT CTTCATGGGT GGCCAGAAAA 
     3901 AGGAACTGCG CTTCAATGAG AATTACTGGT CACTCTTCAA GCTGACGTGC AACAAACCTT 
     3961 GCTGTGACTC GGGGGATGCA ATTTACTACT GTGCCACCTG CTCTGAGGAC CCAGGCAGCA 
     4021 TCTATGCTGT GAAAATCTGC AAAACCCCAG AACCCAAATC AGCCTCATAC TGCAGCCCAT 
     4081 CTGTGCCGGT GCATTTCAAC ATCCAGCAAG ACTGCGGCCA CTTTGTGGCC TCTGTGCCCT 
     4141 CCAGCATGCT TGCCTCCCCT GACACATCCA GCAAAGACAC CGCGCCTGCC GTATCCCCAC 
     4201 AGCCCCCTGC CCAGGAGCAG GACTGTGTGG TGGTCATCAC CCGGGAGGTA CCGCACCAGA 
     4261 CTGCCTCCGA CTTTGTGCGG GACTCTATGG CCAGCCACCG GGCGGAGCCT GAGGTTTACG 
     4321 AGCGCCGGGT GTGCTTCCTG CTTCTGCAGC TCTGCAACGG GCTGGAGCAC CTGAAGGAAC 
     4381 ACGGGATCAT CCACCGGGAC CTGTGTCTGG AGAACCTGCT GCTGGCGCAC TGTAACCCTC 
     4441 AGAGCTCCCC CGGGCCCTCT GCCACTCCTA CCGTGCCCAC CACCACATCC AGGTGCCCTT 
     4501 CTGCCGCGCC CGCAGCCACC ACTGCCTGCC AGGGAGGGCC TGGTGAGAAG CAGCTGCCCA 
     4561 GGCTCATCAT CAGCAACTTC CTGAAGGCCA AGCAAAAGCC AGGAGGCACC ACCAACCTGC 
     4621 AACAGAAGAA GAGCCAGGCC CGGCTGGCTC CCGAGATCGT GTCTGCCTCC CAGTACCGCA 
     4681 AGTTTGATGA GTTCCAGACG GGCATCCTCA TCTATGAGCT GCTCCACCAG CCCAACCCAT 
     4741 TCGAGGTGCG AGCCCAGCTC CGGGAGCGCG ACTACCGGCG GGAGGACTTA CCCCCGCTGC 
     4801 CCACACTGTC CCTCTACTCT CCCGGCCTGC AGCAACTCGC CCATCTACTG CTTGAGGCTG 
     4861 ATCCCATCAA GCGCATCCGC ATAGGTGAAG CTAAGCGGGT GCTGCAGTGC TTGCTGTGGG 
     4921 GGCCACGGCG GGAGCTGGTG GAGCAGCCGT GCACGTCAGA GGAGGTACTG TGTAACACTC 
     4981 TGCACAACTG GATCGACATG AAGCGAGCAC TGATGATGAT GAAGTTTGCT GAGAAGGCGG 
     5041 TGGACCGAAG GCGGGGTGTG GAACTGGAGG ACTGGCTTTG CTGCCAGTAC TTGGCCTCGG 
     5101 CAGAGCCAGG GGCCCTCTTG CAGTCCCTGA AGCTCCTACA ACTTCTCACG CGTACGCGGC 
     5161 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5221 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5281 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5341 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5401 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5461 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5521 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5581 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5641 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5701 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5761 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5821 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5881 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5941 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6001 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6061 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6121 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6181 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6241 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6301 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6361 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6421 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6481 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6541 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6601 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6661 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6721 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6781 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6841 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6901 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6961 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7021 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7081 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7141 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7201 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7261 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7321 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7381 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7441 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7501 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7561 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7621 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7681 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7741 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7801 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7861 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7921 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7981 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8041 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8101 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8161 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8221 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8281 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8341 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8401 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8461 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8521 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8581 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8641 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8701 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8761 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8821 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8881 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8941 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//