LOCUS       MR211754                8406 bp    DNA     circular  SYN 
DEFINITION  Glg1 (Myc-DDK-tagged) - Mouse golgi apparatus protein 1 (Glg1)
ACCESSION   NM_009149
VERSION     NM_009149.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4563..4654 
                     /label="Myc-DDK"  
     misc_feature    4684..5269 
                     /label="PolyA signal"  
     misc_feature    4741..4760 
                     /label="Restriction Enzyme"  
     misc_feature    5545..6217 
                     /label="ColE1"  
     misc_feature    6647..7441 
                     /label="Kan/Neo"  
     misc_feature    7476..7778 
                     /label="SV40 ori"  
     misc_feature    7864..8341 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4545..4551 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4545 
                     /label="Glg1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGTGT GGACGTGTAC GGGGGATGTT CCGCTTGTCG GCGGCGTTGC 
     1081 CGCTGCTGCT GCTCGCGGCA GCAGGGGCCC AGAATGGTCA CGGTCAGGGC CAGGGTCCGG 
     1141 GGACCAACTT TGGGCCCTTC CCAGGGCAAG GTGGAGGCGG CAGCCCGGCC GGCCAACAGC 
     1201 CGCCTCAGCA GCCTCAGTTA TCGCAGCAGC AGCAGCAGCC GCCGCCTCAG CAGCAGCAGC 
     1261 AGCAACAGCA GCAGTCGCTT TTCGCGGCGG GCGGGCTCCC GGCCCGGCGG GGCGGAGCGG 
     1321 GGCCCGGTGG GACTGGCGGA GGCTGGAAGC TGGCGGAGGA AGAGTCCTGC CGGGAGGACG 
     1381 TGACCCGTGT GTGCCCCAAA CACACCTGGA GCAACAACCT GGCGGTGCTC GAGTGCCTGC 
     1441 AGGACGTGAG GGAGCCCGAG AACGAGATTT CTTCAGACTG CAATCATTTG TTGTGGAATT 
     1501 ACAAGCTGAA CCTGACTACT GATCCCAAAT TTGAATCTGT GGCCAGGGAG GTTTGCAAGT 
     1561 CAACTATTTC CGAGATTAAA GAATGTGCGG AAGAACCAGT TGGAAAAGGT TACATGGTTT 
     1621 CCTGCTTGGT GGATCATCGA GGCAACATCA CTGAGTATCA GTGTCATCAA TACATTACCA 
     1681 AGATGACGGC CATCATTTTC AGTGATTATC GCTTGATCTG CGGCTTCATG GATGACTGCA 
     1741 AAAATGATAT CAACCTCTTG AAATGTGGCA GCATTCGTCT TGGGGAAAAG GATGCACATT 
     1801 CACAAGGTGA GGTGGTATCA TGTTTGGAAA AAGGCCTGGT AAAAGAGGCA GAAGAAAAAG 
     1861 AGCCGAAGAT TCAAGTCTCA GAACTCTGCA AGAAAGCCAT TCTTAGGGTG GCTGAGCTGT 
     1921 CCTCGGATGA CTTTCATTTA GACCGGCATT TGTATTTTGC TTGCCGAGAT GATCGGGAGC 
     1981 GCTTTTGCGA GAATACACAA GCTGGTGAAG GAAGAGTGTA TAAATGCCTA TTTAACCATA 
     2041 AGTTTGAAGA ATCCATGAGT GAAAAGTGTA GAGAAGCACT AACTACACGC CAGAAGCTCA 
     2101 TTGCCCAGGA TTATAAAGTC AGTTACTCAT TAGCCAAATC CTGTAAGAGT GACTTGAAAA 
     2161 AATACCGGTG CAATGTGGAA AACCTTCCTC GGTCCCGGGA AGCCAGGCTC TCCTACCTTC 
     2221 TGATGTGCCT GGAGTCAGCA GTGCACAGAG GGCGGCAGGT GAGCAGTGAG TGCCAAGGTG 
     2281 AGATGCTGGA TTACCGACGC ATGCTGATGG AAGACTTCTC TCTGAGCCCC GAGATCATCC 
     2341 TGAGCTGTCG AGGGGAGATT GAACACCATT GTTCTGGATT ACATCGGAAA GGGCGAACCC 
     2401 TCCACTGTCT GATGAAAGTG GTTCGGGGTG AAAAGGGGAA CCTTGGAATG AACTGCCAAC 
     2461 AGGCGCTTCA GACACTGATT CAGGAGACTG ACCCTGGTGC AGACTACCGC ATTGATCGAG 
     2521 CTTTGAATGA AGCTTGTGAG TCTGTAATAC AGACAGCCTG CAAACACATA CGATCCGGAG 
     2581 ACCCAATGAT TCTCTCATGT CTGATGGAGC ATTTATACAC AGAGAAGATG GTGGAAGACT 
     2641 GTGAACACCG GCTCTTAGAG CTACAGTATT TTATCTCTCG GGATTGGAAG TTGGACCCTG 
     2701 TTTTATACCG AAAATGCCAG GGAGATGCTT CCCGCCTTTG CCATACCCAT GGTTGGAATG 
     2761 AGACCAGTGA ACTTATGCCC CCTGGAGCTG TGTTTTCTTG CCTATACAGA CATGCCTACC 
     2821 GCACAGAAGA GCAAGGAAGG AGGCTCTCAC GAGAATGTCG AGCTGAAGTC CAGAGGATCC 
     2881 TGCACCAGCG AGCCATGGAT GTTAAGCTGG ATCCTGCCCT CCAGGACAAG TGCCTCATAG 
     2941 ACCTAGGGAA GTGGTGCAGT GAGAAGACGG AGACTGGGCA GGAGCTTGAG TGCCTTCAGG 
     3001 ACCATCTCGA TGACTTAGCT GTGGAATGCA GAGACATCGT GGGCAACCTC ACCGAGTTAG 
     3061 AGTCCGAGGA TATACAAATA GAAGCTTTGC TGATGAGAGC CTGTGAGCCT ATCATTCAGA 
     3121 ACTTTTGCCA CGATGTGGCA GACAACCAGA TCGACTCTGG GGACCTGATG GAGTGTCTGA 
     3181 TCCAGAACAA GCACCAGAAG GACATGAACG AGAAGTGTGC CATTGGCGTC ACTCACTTCC 
     3241 AGCTGGTACA GATGAAGGAT TTTCGATTCT CTTACAAGTT CAAAATGGCC TGCAAGGAGG 
     3301 ATGTGTTAAA GCTTTGCCCC AACATAAAAA AGAAGGTGGA CGTAGTGATC TGCCTGAGCA 
     3361 CCACTGTGCG CAACGACACT CTGCAGGAGG CCAAGGAGCA CCGAGTATCA CTCAAGTGCC 
     3421 GCAAGCAGCT TCGTGTGGAA GAGCTGGAGA TGACAGAGGA CATCCGTTTG GAACCAGATC 
     3481 TGTATGAAGC CTGCAAGAGT GACATCAAGA ACTACTGTTC TACAGTGCAA TATGGAAATG 
     3541 CTCAGATTAT TGAATGTCTA AAAGAAAACA AGAAGCAGCT GAGTACCCGT TGCCACCAGA 
     3601 AAGTATTTAA GCTGCAGGAG ACGGAGATGA TGGACCCAGA GCTAGACTAT ACTCTGATGA 
     3661 GAGTCTGCAA GCAGATGATT AAGCGGTTCT GTCCAGAAGC AGATTCTAAA ACTATGTTGC 
     3721 AGTGTTTAAA ACAAAATAAG AACAGTGAAT TGATGGATCC CAAATGCAAA CAGATGATAA 
     3781 CCAAGCGCCA GATCACCCAG AACACAGATT ACCGCTTAAA CCCTGTGCTA AGGAAGGCCT 
     3841 GTAAAGCTGA CATTCCTAAG TTCTGTCATG GCATCCTGAC CAAGGCTAAG GACGATTCAG 
     3901 AGCTAGAAGG CCAAGTTATC TCATGCCTCA AGCTGAGATA TGCTGACCAG CGCCTGTCCT 
     3961 CAGACTGTGA GGACCAGATC CGTATCATCA TCCAGGAGTC TGCTCTGGAT TACCGCCTGG 
     4021 ACCCTCAGCT CCAGCTGCAC TGCTCAGATG AGATTGCCAA TCTATGTGCT GAAGAAGCAG 
     4081 CAGCCCAGGA GCAAACAGGC CAAGTGGAAG AGTGCCTGAA GGTCAACCTG CTCAAGATCA 
     4141 AGACAGAGCT GTGTAAAAAG GAAGTGTTAA ACATGTTGAA GGAAAGCAAA GCAGACATCT 
     4201 TTGTGGACCC CGTTCTTCAC ACAGCATGTG CCTTGGACAT TAAACACCAC TGTGCAGCCA 
     4261 TCACCCCTGG CCGCGGGCGT CAGATGTCCT GCCTGATGGA GGCCCTGGAA GATAAGCGAG 
     4321 TGAGATTGCA GCCAGAGTGC AAAAAGCGCC TCAATGACCG GATTGAGATG TGGAGTTATG 
     4381 CAGCCAAGGT GGCTCCAGCA GATGGCTTCT CTGATCTTGC CATGCAAGTG ATGACATCTC 
     4441 CCTCAAAGAA CTACATCCTG TCTGTGATCA GCGGGAGCAT CTGCATACTC TTCCTGATTG 
     4501 GCCTGATGTG TGGACGGATC ACCAAGAGAG TGACACGAGA GCTGAAGGAC AGGACGCGTA 
     4561 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4621 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4681 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4741 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4801 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4861 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4921 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4981 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5041 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5101 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5161 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5221 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5281 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5341 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5401 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5461 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5521 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5581 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5641 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5701 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5761 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5821 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5881 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5941 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6001 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6061 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6121 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6181 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6241 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6301 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6361 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6421 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6481 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6541 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6601 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6661 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6721 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6781 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6841 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6901 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6961 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7021 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7081 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7141 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7201 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7261 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7321 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7381 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7441 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7501 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7561 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7621 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7741 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7801 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7861 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7921 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7981 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8041 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8101 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8161 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8221 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8281 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8341 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8401 AATTTT
//