LOCUS       MR211746                8397 bp    DNA     circular  SYN 
DEFINITION  Thbs2 (Myc-DDK-tagged) - Mouse thrombospondin 2 (Thbs2)
ACCESSION   NM_011581
VERSION     NM_011581.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4554..4645 
                     /label="Myc-DDK"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    4732..4751 
                     /label="Restriction Enzyme"  
     misc_feature    5536..6208 
                     /label="ColE1"  
     misc_feature    6638..7432 
                     /label="Kan/Neo"  
     misc_feature    7467..7769 
                     /label="SV40 ori"  
     misc_feature    7855..8332 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4542 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="Thbs2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCTGGGCA CTGGCCCTGC TGGCTCTGGG CATAGGGCCA AGAGCTTCTG 
     1081 CTGGTGACCA CGTCAAGGAC ACTTCATTTG ACCTTTTCAG CATCAGCAAC ATTAACCGGA 
     1141 AGACCATCGG TGCCAAGCAG TTCCGAGGGC CTGACCCCGG GGTGCCCGCC TACCGTTTTG 
     1201 TACGGTTTGA CTACATCCCC CCAGTGAACA CAGATGATCT CAACAGGATT GTCAAGCTTG 
     1261 CAAGGAGAAA GGAGGGCTTC TTCCTCACAG CCCAACTGAA GCAGGACCGC AAGTCTCGGG 
     1321 GAACGCTCCT GGTGTTGGAA GGCCCCGGCA CCTCCCAGAG GCAGTTTGAG ATTGTGTCCA 
     1381 ATGGCCCAGG GGACACTTTG GACCTCAACT ACTGGGTAGA AGGCAATCAG CATACCAACT 
     1441 TCCTGGAGGA TGTGGGCCTG GCTGACTCCC AGTGGAAGAA TGTGACTGTG CAGGTGGCCA 
     1501 GTGACACCTA TAGCCTGTAT GTGGGCTGCG ATCTTATCGA CAGTGTCACC CTGGAAGAAC 
     1561 CATTCTATGA GCAGCTAGAA GTAGACAGGA GCAGGATGTA CGTGGCCAAA GGTGCATCTC 
     1621 GAGAGAGTCA CTTCAGGGGC TTGCTGCAGA ATGTCCATCT CGTGTTTGCA GATTCTGTGG 
     1681 AAGATATCTT AAGCAAGAAA GGCTGTCAAC ACAGCCAGGG AGCTGAAGTC AACACCATCA 
     1741 GTGAACATAC AGAGACTCTC CATCTGAGCC CTCACATCAC CACAGATCTC GTGGTCCAGG 
     1801 GTGTGGAGAA GGCACAGGAG GTGTGTACGC ACTCCTGCGA GGAGTTGAGC AACATGATGA 
     1861 ATGAGCTCTC TGGACTGCAC GTCATGGTGA ACCAGCTGAG CAAGAACCTG GAGAGAGTGT 
     1921 CTAGTGATAA CCAGTTCCTT TTGGAGCTCA TTGGGGGCCC TCTGAAGACA AGAAACATGT 
     1981 CAGCCTGTGT GCAGGAGGGC CGAATCTTTG CAGAAAATGA AACCTGGGTT GTGGATAGTT 
     2041 GTACCACATG CACCTGCAAG AAATTTAAAA CAGTCTGCCA TCAGATCACC TGCTCACCTG 
     2101 CAACTTGTGC CAACCCATCT TTTGTGGAAG GCGAGTGCTG TCCATCCTGT TCACACTCTG 
     2161 CAGACAGTGA TGAGGGCTGG TCTCCGTGGG CAGAGTGGAC CGAGTGTTCT GTCACCTGTG 
     2221 GCTCTGGGAC CCAGCAGAGA GGCCGGTCTT GTGATGTCAC CAGCAACACC TGCCTGGGCC 
     2281 CCTCCATTCA GACAAGGACA TGCAGCCTGG GCAAATGTGA TACGAGAATC CGTCAGAATG 
     2341 GAGGCTGGAG TCACTGGTCA CCCTGGTCTT CATGCTCCGT GACTTGTGGA GTTGGCAATG 
     2401 TCACCCGCAT ACGTCTCTGC AACTCACCAG TGCCCCAGAT GGGTGGCAAG AACTGCAAGG 
     2461 GCAGCGGCCG GGAAACCAAA CCCTGTCAGC GTGATCCGTG CCCAATTGAT GGCCGCTGGA 
     2521 GCCCCTGGTC CCCTTGGTCA GCCTGCACAG TTACCTGTGC TGGAGGGATC CGTGAGCGCT 
     2581 CACGTGTTTG CAACAGCCCT GAGCCCCAGT ATGGAGGGAA GGACTGTGTC GGGGATGTGA 
     2641 CAGAACACCA AATGTGCAAC AAGAGAAGCT GCCCTATTGA TGGGTGCTTA TCCAACCCGT 
     2701 GTTTTCCTGG AGCCAAGTGC AACAGCTTCC CTGATGGGTC CTGGTCCTGT GGCTCCTGCC 
     2761 CAGTGGGCTT TCTGGGCAAT GGTACCCACT GTGAGGACCT GGATGAGTGT GCTGTGGTCA 
     2821 CAGATATTTG CTTCTCAACT AACAAAGCTC CCCGCTGTGT CAACACCAAC CCGGGCTTCC 
     2881 ACTGCCTGCC TTGTCCACCA CGCTACAAGG GGAACCAACC CTTCGGTGTT GGCCTGGAGG 
     2941 ATGCTAGGAC AGAAAAACAA GTGTGTGAGC CAGAGAATCC ATGTAAGGAC AAGACTCACA 
     3001 GCTGCCACAA GAATGCAGAG TGCATCTACC TGGGCCACTT TAGTGACCCC ATGTACAAGT 
     3061 GTGAGTGCCA GATTGGCTAC GCAGGTGATG GGCTCATCTG CGGGGAGGAC TCAGACCTGG 
     3121 ATGGCTGGCC CAACAACAAC CTGGTGTGTG CTACTAATGC CACCTACCAC TGCATCAAGG 
     3181 ACAACTGCCC CAAACTGCCA AATTCCGGGC AGGAGGATTT TGATAAGGAT GGAATCGGAG 
     3241 ATGCTTGTGA CGAGGACGAT GACAATGACG GTGTGAGCGA TGAGAAGGAC AATTGCCAGC 
     3301 TTCTCTTCAA TCCCCGTCAA TTAGACTATG ACAAGGATGA GGTTGGAGAC CGCTGTGACA 
     3361 ACTGCCCCTA TGTGCACAAC CCAGCACAGA TCGACACAGA CAACAATGGC GAGGGGGATG 
     3421 CCTGCTCTGT GGACATTGAC GGAGACGATG TTTTCAATGA GCGAGACAAT TGTCCATATG 
     3481 TCTACAACAC TGACCAGAGA GATACGGATG GTGATGGCGT GGGTGACCAC TGTGACAATT 
     3541 GTCCTCTGAT GCACAACCCA GATCAGATCG ATCAGGACAA TGATCTCGTT GGAGACCAGT 
     3601 GTGACAACAA TGAGGACATA GATGATGACG GCCACCAGAA CAACCAAGAC AACTGCCCAT 
     3661 ACATCTCCAA CTCCAACCAG GCTGACCATG ACAACGACGG CAAGGGCGAT GCCTGCGACT 
     3721 CTGATGATGA CAATGATGGT GTTCCAGATG ACAGGGACAA CTGTCGGCTT GTGTTCAACC 
     3781 CAGACCAGGA AGACTCGGAC GGTGACGGCC GAGGTGACAT TTGTAAAGAT GACTTTGACA 
     3841 ATGATAATGT CCCAGATATT GATGATGTGT GCCCTGAGAA CAATGCCATC ACTGAGACAG 
     3901 ACTTCAGAAA CTTCCAGATG GTCCCTCTGG ATCCCAAGGG GACCACACAA ATTGATCCCA 
     3961 ACTGGGTAAT TCGTCACCAA GGCAAAGAGC TGGTGCAGAC AGCAAACTCA GACCCTGGCA 
     4021 TCGCTGTAGG TTTCGACGAG TTTGGGTCTG TGGACTTCAG TGGCACTTTC TATGTCAACA 
     4081 CTGACCGGGA TGATGACTAC GCTGGCTTTG TCTTTGGCTA TCAGTCAAGC AGCCGCTTCT 
     4141 ATGTGGTGAT GTGGAAGCAG GTGACCCAGA CCTACTGGGA AGACAAGCCC AGTCGGGCTT 
     4201 ACGGCTACTC TGGTGTGTCA CTCAAAGTGG TAAACTCCAC GACTGGTACT GGCGAGCACC 
     4261 TGAGGAATGC CCTGTGGCAC ACGGGAAACA CAGAAGGCCA GGTCCGGACT CTATGGCATG 
     4321 ACCCCAAAAA CATTGGCTGG AAAGACTACA CTGCCTACAG GTGGCACCTG ATTCACAGGC 
     4381 CTAAGACAGG CTACATGAGA GTCTTAGTGC ATGAAGGAAA GCAAGTCATG GCTGACTCAG 
     4441 GACCAATTTA TGACCAAACC TACGCTGGTG GACGGCTGGG CCTGTTTGTC TTCTCCCAAG 
     4501 AGATGGTCTA TTTCTCGGAC CTCAAGTATG AGTGCAGAGA TGCCACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//