LOCUS       MR211741                8385 bp    DNA     circular  SYN 
DEFINITION  Lamb3 (Myc-DDK-tagged) - Mouse laminin, beta 3 (Lamb3)
ACCESSION   NM_008484
VERSION     NM_008484.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4542..4633 
                     /label="Myc-DDK"  
     misc_feature    4663..5248 
                     /label="PolyA signal"  
     misc_feature    4720..4739 
                     /label="Restriction Enzyme"  
     misc_feature    5524..6196 
                     /label="ColE1"  
     misc_feature    6626..7420 
                     /label="Kan/Neo"  
     misc_feature    7455..7757 
                     /label="SV40 ori"  
     misc_feature    7843..8320 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4524..4530 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4524 
                     /label="Lamb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGCATTT TTCCTCCTGT GGCTTGCCTT GCCTGGCTTC CTGTGTGCCC 
     1081 AGCAAGCCTG CTCCCGAGGG GCCTGTTATC CACCTGTGGG GGACTTGCTC ATTGGAAGGA 
     1141 CTCAGCTTCT TCGAGCCTCA TCTACCTGTG GATTGACCAA ACCTGAGACC TATTGCACCC 
     1201 AATATGGACA GTGGCAGATG AAATGCTGCA AGTGTGACTC CAGGCTGCCT CGAAATTACA 
     1261 ACAGTCACCG AGTGGAGAAT GTCGCATCGT CTTCAGGCCC CATGCGCTGG TGGCAGTCAC 
     1321 AGAATGATGT GAGCCCTGTC TCTCTGCAGC TGGACCTAGA CAAGAGGATG CAGCTTCAGG 
     1381 ACATCATGAT GGATTTTAAG GGTCTCACAC CAGCTGGCAT GTTGATTGAG CGCTCTTCTG 
     1441 ACTTTGGCAA GACATGGAGG GTGTACCAGT ACCTGGCGAC AGACTGTGCC AGCACCTTTC 
     1501 CCCAGGTCCA CCAGGGCCAG CCCAAGAACT GGCAGGACGT CCGGTGCCGG CCCTTGTCCC 
     1561 AGAGGCCTAA TGGGCATCTG ACTGGGGGAA AGGTCCAACT TAACCTTATG GATTTAGCAT 
     1621 CAGCTATCCC TGCATCTCAA AGTAAAAAGA TTCAAGAACT TGGGGACATC ACAAACTTGA 
     1681 GAGTCAACTT CACCAAGCTG GCCCCTGTGC CCCAGAGGGG CTCCTATCCA CCCAGCGCCT 
     1741 ACTTTGCAGT GTCACAGCTA CGTCTACAGG GGAGTTGCTT CTGTCACGGA CATGCCGACC 
     1801 GCTGTGCCCC TAATCCTGGA GGTTCTACCA CTGCTGTGCA GGTCAACAAT GTCTGTGTCT 
     1861 GCCAGCATAA CACAGCTGGC CCCAATTGTG ACCGCTGTGC CCCTTTCTAC AACAACCGTC 
     1921 CATGGAGACC TGCAGAGGGC CAGGACACCC ACGAGTGCCA AAGGTGTGAC TGCAATGGGC 
     1981 ACTCAGAGAC CTGCCACTTC GACCCAGCTG TGTTTGCTGC CAGCCAGGGG ACAAATGGAG 
     2041 GTGTGTGTGA CAACTGCCGG GACCACACCG AGGGCAAGAA CTGCGAGCGC TGTCAGCTAC 
     2101 ACTATTTCCG GAATAGACGA CCCAGCGCTC CCATTCATGA GACTTGTATT CCCTGTGAGT 
     2161 GTGATCCAGA TGGAGCAGTC CAGGGAGCTC CCTGTGACCG ACTGACGGGC CAGTGTGTGT 
     2221 GCAAAGAGTA CGTGCAAGGA GAGCGCTGCG ACCTTTGCAA GCCTGGCTTC ACCGGGCTCA 
     2281 CCTTCGCCAA CCCGAAGGGC TGCCATGCCT GTGACTGCAG TATCTTAGGT GCCCGGAAAG 
     2341 ATATGCCTTG TGAGGAGGAA ACCGGACGCT GCTTGTGTCT GCCCAATGTA GTGGGCCCCA 
     2401 AATGTGACCA GTGTGCCCCT TCCCACTGGA AGCTGGCCAG CGGCCTGGGC TGCGAGCCCT 
     2461 GTGCCTGTGA CCCTCGCAAC TCCCTAAGCT CCCAGTGCAA CCAGTTTACA GGACAGTGTC 
     2521 CTTGTCGGGA AGGGTTTGGC GGCCTCACGT GTAGTTCTGC AGCCATCCGC CAGTGTCCTG 
     2581 ACCAGACCTA TGGACACGTG GCCACAGGGT GCCGAGCTTG TGACTGTGAC TTCAGAGGAA 
     2641 CAGAGGGTCC TGGCTGTGAC AAAGCCTCAG GCCGTTGCCT CTGCCGCCCT GGCTTTACCG 
     2701 GGCCCCGCTG TGACCAATGC CAACGAGGTC ACTGTGACCG CTACCCAGTA TGTGTGGCCT 
     2761 GCCACTCCTG CTTCCAGGCC TATGACACAG ACCTCCAGGA GCAAGCTCGA CGCCTTCACA 
     2821 GTCTCCGTAA TGCCACCGAA GGCCTGTGGA CAGGGACAGG TCTGGAAGAC CATGGCCTGG 
     2881 CTTCTCGGTT GCTAGATGCC AAGAGCAAGA TTGAGCAGAT CAGACAGATT CTGGAAGGCA 
     2941 CTTCTGTCAC AGAGCAGGAT GTGGCTCAGG TGGCCAATGG CATCTTGTCT ATCAGGAGAA 
     3001 CTCTTCAGGG CCTGCCGCTG GACCTGCCCT TAGAGGAGGA GATGGAATCC TTCTCTGGAG 
     3061 ACCTGGGGAA TCTAGACAGA AGCTTCAGTC GGCTCCTCCT TATGTACCGA AGCAAGAAGG 
     3121 AGCAATTTGA GAAGCTAAGC AGTGAAGACC CTTCAGGAGC CTTCCGCATG CTGACCATGG 
     3181 CTTATGAGCA GTCCTCCCGG GCTGCACAGC AAGTGTCTGA CAGTTCTAGC CTGCTGAGCC 
     3241 AGTTGAGGGA CAGTCGCAGG GAAGCAGAGG GCCTGGAGAG ACAGGCTGGA GGAGGAGGCA 
     3301 CCGGAGGAGC TCAGCTCATG GCCCTGCGCC TAGAAATGGC TTCGCTGCCT GACTTGACAC 
     3361 CCACCATCAA CAAGCTTTGT GGCAGGTCTA GGCAGACAGC CTGTACTCCA GGAGATTGCC 
     3421 CTGGAGAGCT GTGTCCTCAG GACAATGGTA CAGCTTGTGG CTCTCACTGC AGGGGAGCCC 
     3481 TGCCCAGAGC CAAAGGGGCC TTCCACATGG CAGGGCGGGT AGCTGAGCAG CTACGAAACT 
     3541 TCAACACCCA GCTCCAGCAG ACCAGGCAAA TGATCAGGGC AGCCGAGGAA GCAGCATCAA 
     3601 GGGTCCAAGC CGATGCCCAG CGCCTTGAGA CCCAGGTGAG TACCAGCCGC TTGCTGATGG 
     3661 AGGAAGATGT CCAGCGCACA CGGCTTCTCA TCCAACAGGT CCGGGGCTTT CTCACAGATC 
     3721 CTGACACAGA TGCAGCCACC ATCCAACAGG TCAGCGAGGC AGTGCTGGCT CTCTGGCTGC 
     3781 CCACAGACTC AGCCACAGTG CTGCGCAAGA TGAAAGAGAT CCAGGCCATT GCGGCCAGGC 
     3841 TCCCTAATGT GGACTCAGTG CTATCCCAGA CCAAACAAGA CATCGCACGG GCCCGCAGGC 
     3901 TCCAGGCTGA GGCTGAGCAG GCCAGAAGCC GAGCCCACGC TGTGGAAGGG CAGGTAGATG 
     3961 ATGTGGTCGG GAACCTTCGG CAGGGCACCG TGGCTCTGCA GGAAGCTCAG GACACAATGC 
     4021 AGGGTACTGG CCGCTCTCTT CGGCTCATCC AGGAAAGGGT TGGTGAGGTT CAGCAGGTCC 
     4081 TTGTACCAGC TGAAAGGCTG GTGAAAGGCA TGAAGGAACA GATGAGTGGA TTCTGGGCAC 
     4141 GGATGAAGGA GCTTCGCCGT CAGGCCCAGG AGGAGCAGGC CCAGGCAATG CAGGCCCGGC 
     4201 AGCTTGCAGA GGGTGCCAGC CAGCAGGCAA TGAATGCCCA GGAGGGCTTT AAGAGACTTA 
     4261 AGCAAAAGTA TACAGAGTTG AAGGACCGGC TGGGTCAGAG TCCTGTGCTG GGCGAGCAAG 
     4321 GCAATCGAAT CCTGAGCATC AAGATGGAGG CAGAGGAGCT CTTTGGGGAG ACCATGGAAA 
     4381 TGATGGACAA AATGAAAGAC ATGGAGTCAG AGCTGCTTCG AGGGAGTCAG GCTATCATGC 
     4441 TGCGCTCTGC AGACCTGTCG GGGCTGGAGA AGCGTGTGGA GCAGATCCGC AGTTACATCA 
     4501 ATGGGCGAGT GCTCTACTAT GCCACCTGCA AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4561 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4621 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4681 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4741 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4801 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4861 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4921 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4981 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5041 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5101 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5161 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5221 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5281 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5341 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5401 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5461 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5521 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5581 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5641 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5701 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5761 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5821 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5881 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5941 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6001 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6061 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6121 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6181 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6241 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6301 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6361 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6421 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6481 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6541 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6601 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6661 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6721 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6781 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6841 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6901 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6961 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7021 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7081 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7141 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7201 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7261 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7321 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7381 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7441 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7501 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7561 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7621 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7801 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7861 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7921 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7981 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8041 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8101 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8161 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8221 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8281 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8341 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//