LOCUS       MR211738                8412 bp    DNA     circular  SYN 
DEFINITION  Polr2b (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (Polr2b)
ACCESSION   NM_153798
VERSION     NM_153798.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4567..4658 
                     /label="Myc-DDK"  
     misc_feature    4688..5273 
                     /label="PolyA signal"  
     misc_feature    4745..4764 
                     /label="Restriction Enzyme"  
     misc_feature    5549..6221 
                     /label="ColE1"  
     misc_feature    6651..7445 
                     /label="Kan/Neo"  
     misc_feature    7480..7782 
                     /label="SV40 ori"  
     misc_feature    7868..8345 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4542..4549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4542 
                     /label="Polr2b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGACGCC GACGAGGACA TGCAATATGA TGAAGACGAC GATGAGATCA 
     1081 CTCCGGATCT GTGGCAGGAA GCATGCTGGA TCGTGATCAG TTCTTACTTT GATGAGAAAG 
     1141 GCTTGGTCAG ACAACAGCTG GATTCCTTTG ATGAGTTTAT TCAGATGTCT GTTCAAAGAA 
     1201 TTGTGGAAGA TGCACCACCG ATTGACCTAC AGGCCGAAGC TCAGCACGCC AGTGGAGAAG 
     1261 TTGAAGAGCC GCCAAGATAT TTACTGAAGT TTGAACAAAT TTACCTCTCC AAACCTACTC 
     1321 ATTGGGAAAG AGATGGTGCC CCTTCGCCAA TGATGCCAAA TGAAGCCAGG TTAAGAAATC 
     1381 TCACGTATTC TGCTCCGCTT TACGTTGACA TAACAAAGAC AGTCATTAAA GAAGGTGAAG 
     1441 AACAGCTGCA GACTCAGCAC CAGAAAACGT TCATAGGGAA AATTCCAATT ATGCTGCGCT 
     1501 CCACCTACTG CCTGTTAAAT GGCTTAACAG ATCGTGACCT GTGTGAGCTG AATGAGTGTC 
     1561 CTTTGGATCC CGGTGGCTAT TTTATTATCA ATGGATCAGA AAAGGTTCTG ATAGCTCAAG 
     1621 AGAAAATGGC AACAAACACA GTCTATGTGT TTGCCAAAAA GGATTCCAAG TATGCCTATA 
     1681 CAGGAGAATG TCGCTCATGC CTGGAGAATT CTTCCCGACC CACCAGTACT ATATGGGTTA 
     1741 GCATGCTGGC CAGAGGAGGA CAGGGTGCAA AGAAGAGTGC TATTGGCCAG CGCATCGTGG 
     1801 CAACGTTACC ATACATCAAG CAAGAAGTTC CTATCATCAT TGTGTTCAGA GCACTAGGCT 
     1861 TTGTGTCTGA TAGAGATATT TTAGAACACA TCATTTATGA CTTCGAGGAT CCAGAGATGA 
     1921 TGGAAATGGT TAAACCATCT CTCGATGAAG CGTTTGTCAT CCAAGAGCAG AATGTTGCCT 
     1981 TAAACTTCAT CGGTTCAAGA GGGGCAAAGC CTGGTGTTAC TAAGGAGAAA AGAATTAAAT 
     2041 ATGCAAAAGA AGTCTTACAG AAAGAAATGC TCCCTCACGT TGGTGTCAGT GATTTCTGTG 
     2101 AGACCAAAAA GGCCTATTTC TTGGGGTATA TGGTTCATAG GTTGCTTCTA GCAGCCCTAG 
     2161 GCAGAAGAGA ACTAGACGAC AGAGATCACT ATGGCAACAA GAGATTGGAT CTTGCTGGGC 
     2221 CTCTGCTTGC ATTCTTATTC AGAGGCATGT TTAAGAATTT GCTGAAAGAA GTACGAATCT 
     2281 ATGCGCAGAA GTTTATTGAT CGCGGGAAGG ATTTTAACTT AGAGCTGGCA ATCAAGACAA 
     2341 GGATCATATC TGATGGGCTA AAATACTCTT TAGCCACTGG GAACTGGGGT GACCAGAAGA 
     2401 AAGCTCATCA AGCCAGAGCT GGAGTATCCC AGGTATTGAA CCGCCTGACG TTTGCATCTA 
     2461 CTCTTTCTCA CCTGCGTCGT CTAAACTCTC CTATTGGCAG AGATGGCAAG CTAGCGAAGC 
     2521 CCAGACAGTT GCATAATACC TTGTGGGGAA TGGTGTGTCC TGCTGAAACT CCAGAGGGCC 
     2581 ATGCTGTAGG GCTTGTGAAG AATTTAGCCC TGATGGCTTA TATTTCAGTT GGATCTCAAC 
     2641 CCTCTCCAAT TTTGGAATTT TTAGAAGAAT GGAGCATGGA AAATTTAGAA GAAATTTCTC 
     2701 CTGCAGCTAT TGCTGATGCA ACCAAGATTT TTGTTAATGG CTGCTGGGTT GGAATCCATA 
     2761 AAGACCCCGA ACAACTCATG AACACGCTAA GGAAGTTACG GCGGCAGATG GACATCATTG 
     2821 TATCCGAAGT TTCTATGATC AGAGATATTC GAGAGCGGGA GATTCGGATC TATACAGATG 
     2881 CAGGCCGTAT TTGTAGACCC CTTCTGATTG TGGAAAAGCA GAAGCTGCTT TTGAAGAAGA 
     2941 GACACATTGA CCAGTTGAAA GAGAGAGAAT ATAACAACTA CAGTTGGCAG GATCTGGTAG 
     3001 CCAGTGGGGT AGTAGAGTAT ATTGATACCC TGGAAGAGGA GACCGTGATG CTTGCAATGA 
     3061 CTCCAGATGA TTTGCAGGAG AAGGAAGTAG CTTATTGTTC TACCTACACC CACTGTGAGA 
     3121 TTCACCCATC AATGATTCTT GGAGTCTGTG CATCTATTAT TCCTTTTCCT GATCATAACC 
     3181 AGTCCCCTAG AAACACATAC CAGTCTGCTA TGGGCAAGCA GGCTATGGGA GTTTATATCA 
     3241 CCAACTTCCA TGTGCGGATG GACACCTTGG CCCATGTTCT CTACTATCCT CAGAAACCCC 
     3301 TTGTGACTAC ACGGTCTATG GAATATCTAC GATTCAGAGA GCTGCCAGCA GGAATCAACT 
     3361 CAATTGTGGC CATTGCATCC TACACGGGCT ATAATCAAGA AGACTCTGTT ATCATGAATC 
     3421 GTTCAGCTGT GGATCGAGGC TTTTTCAGGT CTGTTTTCTA CCGATCATAC AAAGAACAAG 
     3481 AGTCTAAAAA GGGATTTGAT CAAGAAGAAG TGTTTGAGAA GCCTACACGT GAGACATGCC 
     3541 AGGGAATGAG GCATGCCATC TATGAAAAGT TGGACGATGA CGGTTTGATA GCTCCGGGGG 
     3601 TCCGAGTCTC CGGAGATGAC GTTATTATCG GCAAGACAGT CACCTTACCC GAGAACGAGG 
     3661 ATGAGCTGGA GAGCACCAAC AGGCGCTACA CCAAGAGGGA CTGCAGCACT TTCCTCAGAA 
     3721 CTAGTGAGAC GGGCATCGTG GACCAGGTCA TGGTCACTCT CAACCAGGAA GGATATAAAT 
     3781 TTTGTAAAAT AAGGGTGCGC TCTGTTAGAA TTCCGCAGAT TGGAGACAAA TTTGCTAGCC 
     3841 GACATGGTCA GAAGGGAACT TGTGGTATTC AGTATAGACA AGAGGACATG CCGTTCACTT 
     3901 GCGAAGGCAT AACTCCTGAC ATCATCATAA ACCCCCACGC CATCCCCTCC CGGATGACAA 
     3961 TTGGTCACTT GATCGAATGT CTCCAAGGGA AGGTCTCTGC CAACAAGGGT GAAATTGGTG 
     4021 ACGCTACTCC ATTTAATGAT GCTGTCAATG TGCAGAAGAT TTCTAATCTT TTGTCTGACT 
     4081 ATGGCTACCA TCTCAGAGGG AACGAGGTGC TCTACAATGG ATTCACTGGA CGAAAAATCA 
     4141 CATCACAGAT TTTTATTGGC CCCACTTACT ACCAGCGTTT GAAGCATATG GTGGATGACA 
     4201 AAATTCATTC TCGTGCTAGG GGACCCATTC AGATTCTTAA CAGACAGCCC ATGGAGGGCA 
     4261 GATCTCGTGA TGGTGGCCTG CGCTTCGGAG AAATGGAACG AGACTGTCAA ATTGCCCACG 
     4321 GTGCAGCACA GTTTCTAAGA GAAAGACTGT TTGAAGCATC AGATCCGTAT CAGGTTCATG 
     4381 TCTGTAATCT TTGTGGAATA ATGGCAATTG CTAACACGAG GACTCATACA TATGAGTGCA 
     4441 GGGGATGCCG CAATAAAACC CAGATTTCCT TGGTGCGAAT GCCGTACGCG TGCAAGCTGT 
     4501 TGTTCCAGGA GCTCATGTCC ATGAGCATCG CACCACGAAT GATGAGTGTT AGCGGACCGA 
     4561 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4621 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4681 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4741 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4801 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4861 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4921 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4981 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5041 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5101 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5161 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5221 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5281 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5341 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5401 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5461 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5521 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5581 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5641 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5701 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5761 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5821 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5881 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5941 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6001 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6061 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6121 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6181 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6241 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6301 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6361 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6421 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6481 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6541 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6601 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6661 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6721 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6781 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6841 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6901 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6961 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7021 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7081 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7141 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7201 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7261 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7321 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7381 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7441 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7501 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7561 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7621 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7741 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7801 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7861 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7921 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7981 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8041 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8101 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8161 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8221 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8281 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8341 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8401 AACGCGAATT TT
//