LOCUS       MR211737                8382 bp    DNA     circular  SYN 
DEFINITION  Tjp2 (Myc-DDK-tagged) - Mouse tight junction protein 2 (Tjp2), transcript variant 2
ACCESSION   NM_011597
VERSION     NM_011597.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4539..4630 
                     /label="Myc-DDK"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    4717..4736 
                     /label="Restriction Enzyme"  
     misc_feature    5521..6193 
                     /label="ColE1"  
     misc_feature    6623..7417 
                     /label="Kan/Neo"  
     misc_feature    7452..7754 
                     /label="SV40 ori"  
     misc_feature    7840..8317 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4527 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="Tjp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGGTG ATATGGGAGC AGTACACCGT GACCCTGCAG AAGGATTCCA 
     1081 AAAGAGGATT TGGAATTGCC GTGTCGGGAG GCAGAGACAA CCCCCACTTT GAAAATGGGG 
     1141 AGACGTCAAT CGTCATCTCA GATGTGCTCC CAGGTGGACC CGCTGATGGC TTGCTTCAAG 
     1201 AAAATGACAG GGTGGTCATG GTCAATGGCA CCCCCATGGA AGATGTGCTC CATTCGTTTG 
     1261 CCGTTCAGCA GCTTAGGAAA AGTGGGAAGA TAGCAGCCAT CGTGGTCAAG AGACCCCGGA 
     1321 AGGTTCAGGT GGCCCCACTG CAAGGCAGCC CCCCTCTCAG TCATGACGAC CGAGGTTTTG 
     1381 AAGTGATTGA AGAATTTGAT GGCAGAAGTT TCCGCAGCGG CTACAGTGAG AGGAGTCGGC 
     1441 ACAGCAGCCA CGACATGCTC AGCCACAGCT GGGAGGGCAA TAGGGAGAGG GGGCGACCCC 
     1501 ATCAGCGCAC ACAGAGCCGG GAGCGGGAGC GCAGCCGTGG CAGGAGCCTG GAACGGGGCC 
     1561 TGGACCAGGA AGACTATGGG CGCAGCCGTG AGCGCAGCCG TGGCCGGAGC CTGGAGCGCG 
     1621 GGCTGGACCG CGACTTTGTG TCCAGGGACC ACAGCCGTGG CCGTAGCATC GACAGGGACT 
     1681 ACGACCGAGA CTATGAGCGC TCCTATCACG AAGCTTATGA ACCCGACTAC GGAGGCGGCT 
     1741 ACAGCCCATC ATATGACCGT AGAGCCCATC CAGAGACCCG CTATGAACGG AGCCGCAGCC 
     1801 GAGAACACCT ACGTTCCCGA AGCCCCAGCC CCGAGTCTAG GTCACGGCAT GAACACAAGG 
     1861 GCCAACATGA TCCCGACAGG CCCATCGGGG TCCTTCTAAC CAAAAGCAAA GCAAATGAAG 
     1921 AGTATGGGCT CCGGCTGGGG AGTCAGATCT TCATCAAAGA AATGACCAGA ACAGGACTGG 
     1981 CCACCAAAGA TGGCAACCTT CATGAGGGGG ACATAATTCT CAAGATCAAC GGCACTGTTA 
     2041 CTGAGAACAT GTCTCTAACG GATGCTCGGA AGTTAATAGA AAAGTCTCGA GGAAAACTGC 
     2101 AGCTTGTGGT GTTGAGAGAC AGCAAGCAGA CCCTCATCAA CATCCCAGCC CTAAACGACA 
     2161 GCGACTCTGA AGTGGAGGAT ATCTCGGAAA TAGAGTCCAA CCGGTCTTTC TCTCCAGAGG 
     2221 AGAGACGCCA GCAGTATTCT GATCAGGATT ACCATTCCTC CACTGAGAAG CTGAAGGAGA 
     2281 GGCCGAGCTC AAGAGAGGAG ACCTCAGGCA GACTATCCAG GATGGGTGCC ACTCCCACGC 
     2341 CGTTCAAGTC CACGGGGGAC ATCACAGCTG CGGGGGTCAC AGAGGCCAGC AGGGAGCCCA 
     2401 GGTACCAGGA AGAAGGCCCA GTTCCTCAAC CCAGAACAGC TCCAAGAGTT TTTCTTCGTC 
     2461 CTAGTCCCGA AGATGAAGCA ATATATGGCC CTAACACCAA AATGGTGAGG TTCAAGAAGG 
     2521 GAGACAGCGT GGGCCTCCGG TTGGCTGGTG GAAACGACGT TGGGATATTT GTGGCTGGCA 
     2581 TTCAGGAGGG CACCTCCGCA GAGCAGGAGG GCCTGCAAGA AGGAGACCAG ATTCTGAAGG 
     2641 TGAACACACA GGATTTCAGA GGGCTAGTCC GGGAAGATGC GGTCCTCTAC CTGCTAGAAA 
     2701 TCCCCAAAGG TGAAACTGTG ACCATTTTGG CTCAGAGCCG AGCAGACGTG TACAGAGATA 
     2761 TCCTGGCTTG TGGCAGGGGA GATTCGTTTT TCATAAGAAG CCACTTTGAA TGTGAGAAGG 
     2821 AAACTCCCCA GAGCCTGGCC TTCACCAGGG GAGAGGTCTT CCGAGTGGTA GACACGCTGT 
     2881 ACGATGGCAA GCTGGGCCAC TGGCTGGCCG TGAGGATCGG GAATGAGCTG GAGAAAGGCT 
     2941 TGATCCCTAA CAAAAGCAGA GCCGAGCAAA TGGCCAGTGT CCAGAATGCC CAGCGAGAGA 
     3001 ACGCCGGGGA CAGAGCAGAC TTCTGGCGGA TGCGTGGCCA GAGATCCAGC GGCGGCGTCA 
     3061 AGAAGAACCT CCGCAAGAGC CGGGAAGACC TGGCGGCTGC TGTATCGGTT AGCACCAAGT 
     3121 TCCCTGCCTA CGAGAAGGTT CTGCTTCGGG AAGCTGGCTT CAAGAGACCC GTGGTTCTGT 
     3181 TTGGCCCCAT AGCAGATATA GCAATGGAAA GGTTGGCAAC TGAGTTACCA GACCTGTTTC 
     3241 AAACTGCAAA AACAGAACCC AAAGATGCCG GATCTGAGAA ATCCAGTGGA GTGGTTCGGT 
     3301 TGAATACCGT GAGGCAAATT ATTGAGCAGG ACAAGCACGC CCTGCTCGAC GTTACCCCCA 
     3361 AAGCTGTGGA CCTGCTCAAC TACACTCAGT GGTTCCCTAT CGTGATTTTC TTCAACCCGG 
     3421 ATTCCAGACA AGGTGTTAAA ACCATAAGGC AGAGGTTGAG TCCAACATCC AATAAAAGTT 
     3481 CTCGCAAGTT ATTTGATCAA GCCAACAAGC TCAAAAAGAC CTGTTCTCAT CTTTTTACAG 
     3541 CCACAATCAA CGTGAATTCA GCCAACGATG GCTGGTTTGG CAGCTTGAAG GACAGCATTC 
     3601 AGCAGCAGCA AAACGAAGCA GTCTGGGTCT CTGAAGGAAA GATGGAAGGG ATGGATGATG 
     3661 ATGCTGAAGA CCGCATGTCC TACTTAACCG CCATGGGCGC GGACTATCTG AGTTGTGACA 
     3721 GCCGTCTCAT CAGTGACTTT GAAGACACCG ACGGCGAGGG AGGCGCCTAC ACTGACAATG 
     3781 AGCTAGAGGA GCCGGCGGAG GAGCCGCTGG TGTCTTCCAT CACCCGCTCC TCGGAGCCGG 
     3841 TGCAGCACGA GGAGAACATA AGGAAATCCA GCCCAGAGCC ACGAGCTCAG ATGAGGAGGG 
     3901 CTGCTAGCAG AGACCAACTT AGGGATGCTA GCCCGCCCCC AGCCTTCAAG CCAGAGCCGC 
     3961 CCAAGGCAAG AAGCCAGAAC AGAGAAGACT CCTTCGACTA CTCCAAGTCA AACCTCCCCG 
     4021 CCACAGCCGG CAGTGAAATC CCGGGGGGAT CCACCAAAGG CTATCCTCCC CCTATTGCAG 
     4081 CGAAGCCTGC CTTTGGGCGG CCCATCCTGA AGCCTTCCAC TCCAGTCCCT ATGCCTGAGA 
     4141 GCGAGGAGGT TGGAGAGAGC ACCGAGGAGC AGGAAGATGC TCCCAGATCA GTCCTGGGCA 
     4201 GAGTGAAAAT ATTCGAGAAG ATGGACCACA AGGCAAAATT ACAGAGGATG CAGGAGCTCC 
     4261 AGGAGGCCCA GAATGCGAGG ATCGAAATAG CTCAGAAGCA CCCTGACATC TATGCGGTTC 
     4321 CAATCAAGGC CCCCAAGCCA GATGCTGGCC TCCCCCCGCA CATGAGTTCT AGACCCCCAG 
     4381 AGCCCCAGAA AGCTCCTTCC AGACTTTACC AGGACACCAG AGGAAGCTAC GGCAGTGACC 
     4441 CCGAGGAGGA GGAGTACCGC CAGCAGTTGG CAGCACACTC GAAGCGTGGT TACTACAGCC 
     4501 AGCCCTCCCG GTACCGAGAC ACGGAGTTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4561 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4621 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4681 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4741 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4801 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4861 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4921 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4981 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5041 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5101 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5161 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5221 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5281 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5341 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5401 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5461 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5521 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5581 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5641 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5701 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5761 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5821 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5881 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5941 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6001 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6061 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6121 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6181 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6241 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6301 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6361 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6421 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6481 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6541 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6601 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6661 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6721 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6781 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6841 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6901 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6961 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7021 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7081 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7141 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7201 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7261 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7321 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7381 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7441 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7501 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7561 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7621 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7801 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7861 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7921 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7981 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8041 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8101 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8161 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8221 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8281 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8341 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//