LOCUS       MR211734                8376 bp    DNA     circular  SYN 
DEFINITION  Spag5 (Myc-DDK-tagged) - Mouse sperm associated antigen 5 (Spag5)
ACCESSION   NM_017407
VERSION     NM_017407.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4533..4624 
                     /label="Myc-DDK"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    4711..4730 
                     /label="Restriction Enzyme"  
     misc_feature    5515..6187 
                     /label="ColE1"  
     misc_feature    6617..7411 
                     /label="Kan/Neo"  
     misc_feature    7446..7748 
                     /label="SV40 ori"  
     misc_feature    7834..8311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4515..4521 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4515 
                     /label="Spag5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAGGGTG AAAACACTGA ATCTCGGTTT GTCGCCTTCG CCCCAAAAGG 
     1081 GGAAGCCAGC TATGAGTACT CCTCTCCGAG AGCTTAAACT ACAGCCCGAG GCCCTCGCCG 
     1141 ATTCAGGGAA AGGTCCCTCT ATGATCTCCG CGCTGACCCC ATACCTGTGC AGGCTGGAGC 
     1201 TGAAGGAAAG ATGCAACAAC TCATCTCCAG TGGATTTTAT CAATACTGAG AACAACTTCC 
     1261 TTTCAGAACA GTTCAGCCAT CCTTCAACGC ACATAGAAGC TTGCCAGCGT GAATCCGATC 
     1321 CAACTCCTGA AAGCAACTCT CTTTTCCATA CTTTGGAGGA AGCAATAGAA ACAGTGGATG 
     1381 ACTTTGTCGT GGACCCAAGA GATGATAGTA TAGTGGAGTC CATGGTTCTT CTGCCCTTTT 
     1441 CATTAGGGCA GCAGCAAGAC CTGATGCTCC AGGCTCACTT AGATACCACA GCAGAGAGAA 
     1501 CTAAAAGCTC TCTAAATGAA TCTTTGGGGC TAGAAGATCT GGTGGGGAAG GAGGTGGCAC 
     1561 CTTGTGTGGA AGACAGCCTA ACAGAAATTG TTGCTATTAG GCCCGAGCAA CCTACATTTC 
     1621 AGGACCCTCC GTTAGGACCC AGTGACACTG AAGATGCACC TGTGGACTTA GTTCCTTCTG 
     1681 AAAATGTCCT GAATTTCTCT CTGGCTCGTC TTTCTCCTTC AGCTGTCCTG GCCCAGGATT 
     1741 TCTCTGTCGA TCATGTCGAT CCAGGGGAGG AAACCGTAGA GAACAGAGTC CTACAGGAAA 
     1801 TGGAAACGAG CTTCCCCACA TTCCCTGAGG AGGCCGAATT GGGAGACCAA GCACCTGCTG 
     1861 CAAATGCAGA AGCCGTCTCA CCCTTGTACC TGACATCAAG TCTAGTGGAG ATGGGGCCCC 
     1921 GGGAAGCCCC AGGCCCAACA GTAGAAGACG CCAGTAGGAT TCCTGGCCTT GAGTCAGAGA 
     1981 CTTGGATGTC CCCATTGGCC TGGCTAGAAA AGGGTGTGAA TACATCGGTC ATGCTACAAA 
     2041 ACCTCCGCCA GAGCTTATCC TTTTCTTCTG TGCTTCAGGA TGCTGCCGTT GGCAACACAC 
     2101 CCCTCGCCAC GTGTTCTGTG GGCACTTCCT TTACTCCTCC AGCACCACTG GAGGTAGGCA 
     2161 CCAAGGACAG TACTTCAGAG ACAGAGCGCC TCCTCTTGGG CTGCCGTCCT CCAGATCTGG 
     2221 CTACCTTGTC CCGACACGAC TTGGAAGAGA ACCTGTTGAA CTCTCTTGTC TTGCTGGAAG 
     2281 TTCTTTCCCA CCAGCTACAA GCCTGGAAGA GCCAGTTGAC TGTCCCTCAC CGGGAAGCTC 
     2341 GAGACAGTAG TACACAGACT GACAGCTCTC CTTGTGGGGT CACTAAGACA CCTAAACATC 
     2401 TTCAGGACAG CAAGGAGATT AGACAGGCTC TACTGCAAGC CAGGAATGTC ATGCAATCAT 
     2461 GGGGTCTAGT CTCTGGAGAC CTGTTGTCCT TGCTTCACCT GTCCCTAACA CACGTGCAAG 
     2521 AAGGCAGAGT GACAGTGAGT CAGGAGTCTC AGCGGTCAAA AACCCTGGTT TCCTCCTGTT 
     2581 CTCGTGTGCT GAAGAAATTG AAGGCAAAGC TGCAGAGCCT CAAAACAGAA TGTGAGGAGG 
     2641 CAAGGCACAG CAAGGAAATG GCCCTTAAAG GCAAGGCTGC GGCAGAGGCA GTACTGGAGG 
     2701 CTTTCCGTGC ACATGCCAGC CAGCGCATCA GCCAACTGGA ACAGGGTCTC ACATCTATGC 
     2761 AGGAATTCAG AGGCCTTCTA CAGGAGGCAC AGACCCAACT GATAGGGCTT CACACAGAAC 
     2821 AAAAGGAGCT GGCTCAGCAG ACAGTGAGTC TGAGTTCTGC CTTGCAGCAG GACTGGACAT 
     2881 CAGTCCAATT GAATTATGGA ATATGGGCAG CTTTGCTGAG TTGGTCTCGA GAACTCACCA 
     2941 AGAAACTCAC AGCCAAGAGC CGGCAGGCCC TTCAGGAACG TGATGCTGCA ATTGAAGAAA 
     3001 AGAAGCAGGT TGTGAAGGAA GTGGAACAAG TCTCTGCCCA TTTAGAGGAC TGTAAAGGTC 
     3061 AAATCGAACA ACTGAAGTTG GAAAATAGTC GCCTTACTGC AGATCTCTCG GCTCAGCTGC 
     3121 AGATTCTGAC TAGCACAGAG AGTCAGCTAA AGGAAGTACG GAGTCAGCAT TCCCGCTGTG 
     3181 TCCAGGACCT GGCCGTGAAG GATGAATTGC TGTGTCAACT TACCCAGAGC AACAAGGAGC 
     3241 AGGCTACTCA ATGGCAAAAG GAAGAAATGG AACTGAAACA CATACAAGCA GAACTGCTAC 
     3301 AGCAGCAGGC TGTGTTGGCT AAGGAGGTCC AGGACCTGAG GGAGACTGTG GAGTTTATAG 
     3361 ATGAAGAAAG TCAAGTTGCT CACCGGGAGC TGGGCCAGAT TGAGAGTCAG TTGAAAGTCA 
     3421 CGCTAGAGTT ACTGCGGGAG CGCAGCCTGC AGTGTGAGAC CCTCAGGGAC ACTGTGGACA 
     3481 GCCTGAGGGC TGAGCTGGCC AGCACTGAAG CAAAGCATGA GAAACAGGCC CTAGAGAAGA 
     3541 CACACCAGCA TTCTCAAGAG CTGCGGCTAC TGGCTGAGCA GCTGCAGAGT CTCACCCTCT 
     3601 TCTTACAGGC AAAACTCAAG GAGAACAAGG CTGAATCAGA GATCATTCTG CCCAGCACAG 
     3661 GCTCTGCTCC AGCCCAGGAA CACCCTCTGT CCAATGACAG CAGCATCTCA GAACAGACCC 
     3721 CGACAGCAGC AGTAGATGAA GTGCCAGAAC CAGCTCCTGT GCCATTGCTT GGAAGTGTTA 
     3781 AGAGTGCTTT CACCCGAGTA GCCTCAATGG CTTCCTTTCA GCCTACAGAG ACCCCAGACT 
     3841 TGGAGAAGAG CCTGGCAGAA ATGAGTACTG TGTTACAAGA GCTTAAGAGC CTGTGTTCCC 
     3901 TGCTGCAAGA GTCTAAAGAG GAGGCCACTG GGGTCCTGCA GAGGGAAATC TGTGAACTAC 
     3961 ACTCGAGACT ACAGGCCCAA GAAGAAGAGC ATCAGGAAGC CCTGAAGGCA AAGGAAGCAG 
     4021 ACATGGAGAA GCTGAACCAG GCCTTGTGCT TGCTCCGCAA GAATGAGAAG GAGCTCCTGG 
     4081 AAGTGATACA GAAGCAGAAC GAGAAGATCC TGGGGCAAAT AGACAAGAGC GGCCAGCTCA 
     4141 TAAACCTCAG AGAGGAGGTG ACCCAGCTCA CACAGTCACT TCGGCGTGCA GAGACAGAGA 
     4201 CTAAAGTGCT CCAGGAAGCC CTGGAAGGCC AGCTAGATCC CAGCTGCCAG CTGATGGCTA 
     4261 CTAACTGGAT CCAGGAAAAA GTGTTTCTCT CACAGGAGGT GAGCAAGCTG AGGGTTATGT 
     4321 TCCTGGAGAT GAAAACTGAG AAGGAACAGC TGATGGACAA GTATCTGAGC CATAGGCACA 
     4381 TCCTGGAGGA GAATCTTCGG CGCTCTGACA CAGAGTTAAA GAAACTCGAT GACACAATTC 
     4441 AGCATGTCTA TGAGACTCTG TTGTCTATCC CAGAGACTAT GAAGAGTTGC AAGGAGTTAC 
     4501 AAGGATTGCT AGAATTTCTG AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4561 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5341 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5461 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5521 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5581 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5641 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5761 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5821 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5881 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5941 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6181 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6241 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6301 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6361 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6421 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6481 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6541 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6601 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6661 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6721 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6781 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6841 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6901 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6961 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7021 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7081 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7141 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7201 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7261 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7321 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7381 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7441 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7501 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7561 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7621 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7801 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7861 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7921 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7981 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8041 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8101 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8161 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8221 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8281 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8341 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//