LOCUS       MR211730                8373 bp    DNA     circular  SYN 
DEFINITION  Spag9 (Myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 4
ACCESSION   NM_001025430
VERSION     NM_001025430.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4530..4621 
                     /label="Myc-DDK"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    4708..4727 
                     /label="Restriction Enzyme"  
     misc_feature    5512..6184 
                     /label="ColE1"  
     misc_feature    6614..7408 
                     /label="Kan/Neo"  
     misc_feature    7443..7745 
                     /label="SV40 ori"  
     misc_feature    7831..8308 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4512..4518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4512 
                     /label="Spag9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCCTGGA TGCATGCTGC TGTTTGTGTT TGGCTTTGTT GGCGGGGCGG 
     1081 TGGTCATTAA TTCTGCGATC TTAGTATCTC TCTCTGTTTT GCTGCTTGTG CACTTTTCTA 
     1141 TTTCTACCGG GGTGCCAGCG CTGACGCAGA ACCTACCAAG GATACTCAGA AAAGAACGTC 
     1201 CTATATCATT AGGGATTTTC CCTCTACCTG CTGGAGATGG ATTGCTTACA CCTGACACTC 
     1261 AGAAAGGAGG CGAGACCCCA GGATCAGAGC AATGGAAATT TCAAGAATTA AGTCAACCAC 
     1321 GTTCTCATAC CAGCCTGAAG GATGAACTTT CTGATATTAG TCAAGGTGGA TCTAAAGCTA 
     1381 CCACTCCAGC TTCAACAGCA AATTCAGACG TATCAGCAAT TCCTCCTGAT ACTCCGTCAA 
     1441 AGGAAGATAA TGAAGGATTT GTAAAAGGCA CAGATACATC AAATAAGTCA GAGATAAGCA 
     1501 AACACATAGA AGTCCAGGTT GCCCAAGAGA CTAGGAATGT GTCTACAGAA TCTGGTGAAA 
     1561 ATGAAGAGAA ATCAGAAGTT CAAGCAATCA TTGAATCCAC TCCTGAATTG GATATGGACA 
     1621 AAGACCTCAG TGGATATAAA GGTTCAAGCA CTCCCACCAA AGGCATAGAG AACAAAGCTT 
     1681 TTGATCGCAA TACAGAATCT CTCTTTGAAG AGCTGTCCTC AGCTGGCTCG GGCCTTATTG 
     1741 GAGATGTGGA TGAAGGAGCG GATTTACTAG GAATGGGTCG TGAGGTGGAG AATCTCATAC 
     1801 TGGAGAACAC ACAGCTGCTG GAAACCAAAA ACGCTCTGAA TGTAGTGAAG AATGACTTAA 
     1861 TAGCCAAAGT GGATGAGCTG ACCTGTGAGA AGGACGTGCT GCAGGGGGAG CTGGAGGCTG 
     1921 TGAAGCAAGC CAAGCTGAAG CTGGAGGACA AGAACAGAGA GCTGGAGGAG GAGCTCAGGA 
     1981 AAGCTCGTGC AGAAGCTGAA GATGCAAGGC AAAAAGCAAA AGACGATGAT GATAGTGACA 
     2041 TTCCCACAGC CCAGAGGAAG AGGTTTACCA GAGTAGAAAT GGCCCGAGTT CTCATGGAGA 
     2101 GAAACCAGTA CAAAGAGAGG CTGATGGAGC TTCAAGAAGC TGTTCGATGG ACGGAGATGA 
     2161 TCCGGGCCTC ACGAGAGAAC CCAGCTATGC AAGAAAAGAA AAGGTCAAGC ATCTGGCAAT 
     2221 TTTTCAGCCG ACTTTTTAGC TCTTCAAGTA ATGCAACAAA GAAGCCTGAA CCCCCTGTTA 
     2281 ACCTGAAGTA CAACGCCCCC ACCTCTCACG TGACTCCCTC CGTGAAGAAA AGGAGCAGCA 
     2341 CTTTATCTCA GCTCCCTGGG GACAAGTCCA AGGCATTTGA TTTCCTCAGT GAGGAAACTG 
     2401 AGGCTAGTTT AGCCTCCCGC AGAGAGCAAA AGAGAGAGCA GTATCGGCAG GTGAAGGCTC 
     2461 ACGTTCAGAA GGAAGATGGC CGAGTGCAAG CTTTCGGCTG GAGTCTGCCT CAGAAGTACA 
     2521 AACAGGTAGC CAATGGTCAA GGGGAAACTA AGATGAAAAA TTTACCTGTG CCTGTCTATC 
     2581 TCAGACCACT AGACGAAAAA GATGCGTCTA TGAAGCTGTG GTGTGCTGTA GGAGTCAATT 
     2641 TATCTGGTGG CAAGACAAGA GATGGCGGGT CTGTTGTTGG AGCGAGTGTA TTTTACAAGG 
     2701 ATATTGCTGG TTTGGACACT GAAGGCAGTA AACAGCGGAG TGCGTCTCAG AGTAGTTTAG 
     2761 ACAAGTTGGA TCAAGAACTC AAGGAACAGC AGAAAGAATT TAAAAATCAA GAAGAGTTGT 
     2821 CCAGTCAGGT CTGGATCTGT ACCAGCACCC ACTCAACTAC AAAGGTTATC ATCATTGATG 
     2881 CTGTTCAGCC TGGCAACATC CTAGATAGTT TCACTGTTTG CAATTCTCAT GTTCTGTGCA 
     2941 TTGCCAGTGT CCCAGGAGCT CGGGAAACAG ACTACCCTGC AGGAGAAGAA CTTTCTGAAT 
     3001 CTGGTCAGGT AGACAAAGCG TCATTGTGTG GAAGCATGAC CAGTAACAGC TCCGCAGAGA 
     3061 TGGACAGCTT GCTGGGAGGC ATCACAGTGG TTGGGTGCTC CACAGAAGGA CTGACAGGAG 
     3121 CTGCCACTTC CCCAAGTACC AACGGTGCTT CTCCTGTGAT TGAAAAGCCA CCAGAAATGG 
     3181 AAACTGAAAA TAGCGAAGTT GATGAAAATA TTCCAACAGC AGAAGAAGCA ACTGAAGCCA 
     3241 CAGAGGGCAA TGCAGGGTCC ACTGAAGACA CTGTGGACAT CTCCCAGCCT GGCGTGTACA 
     3301 CAGAGCATGT GTTTACAGAT CCACTGGGAG TTCAGATCCC AGAAGACCTC TCCCCAGTGT 
     3361 TTCAGTCAAG TAATGACTCA GATGTGTACA AAGATCAAAT ATCAGTATTG CCAAATGAAC 
     3421 AAGACCTTGC GAGAGAAGAA GCTCAGAAAA TGAGTAGTCT TTTACCAACC ATGTGGCTTG 
     3481 GAGCTCAGAA TGGCTGTTTG TATGTCCACT CATCTGTAGC CCAGTGGAGG AAATGTCTCC 
     3541 ATTCCATTAA GCTCAAAGAC TCAATTCTCA GTATTGTACA TGTGAAAGGA ATTGTACTAG 
     3601 TGGCCCTGGC GGATGGCACC CTTGCAATCT TCCACAGAGG AGTTGATGGA CAATGGGATC 
     3661 TGTCAAACTA TCACCTTTTA GACCTTGGAC GTCCTCATCA TTCCATACGA TGCATGACTG 
     3721 TGGTACATGA CAAAGTCTGG TGTGGCTATA GGAACAAAAT CTATGTGGTT CAACCAAAGG 
     3781 CTATGAAGAT AGAGAAATCA TTTGATGCAC ACCCCAGGAA GGAGAGCCAA GTACGGCAGC 
     3841 TTGCATGGGT GGGTGATGGT GTATGGGTCT CCATTCGTTT GGATTCCACG CTCCGCCTCT 
     3901 ATCATGCACA CACATACCAA CATCTCCAGG ATGTGGATAT TGAGCCTTAT GTAAGCAAAA 
     3961 TGTTAGGTAC TGGAAAACTG GGTTTCTCTT TCGTGAGGAT AACAGCTCTT ATGGTGTCTT 
     4021 GCAATCGTTT GTGGGTGGGG ACTGGAAATG GTGTCATTAT CTCCATCCCA CTGACAGAAA 
     4081 CAAATAAAAC CTCAGGGACT CCAGGTAACC GTCCTGGAAG TGTAATCCGT GTATATGGTG 
     4141 ATGAGAACAG TGATAAAGTG ACTCCAGGGA CATTTATACC CTACTGTTCA ATGGCACATG 
     4201 CACAGCTTTG TTTCCACGGG CACCGGGATG CTGTGAAATT CTTTGTGGCA GTCCCAGGTC 
     4261 AGGTCATTAG CCCACAAAGT AGCAGTGGTG GGGCAGATCT TACAGCTGAC AAGGCAGGGT 
     4321 CATCTGCACA GGAGCCCAGT AGCCAGACGC CCTTGAAGTC CATGCTTGTC ATCAGTGGAG 
     4381 GAGAGGGCTA CATTGACTTC CGGATGGGTG ATGAAGGTGG AGAATCTGAA CTTCTGGGAG 
     4441 AGGATCTTCC ACTGGAACCT TCAGTCACCA AAGCCGAGAG GAGTCACCTG ATAGTGTGGC 
     4501 AAGTGATGTG TGGCAATGAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4561 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5341 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5401 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5461 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5521 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5581 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5641 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5701 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5761 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5821 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5881 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5941 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6001 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6061 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6121 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6181 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6241 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6301 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6361 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6421 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6481 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6541 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6601 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6661 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6721 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6781 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6841 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6901 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6961 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7021 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7081 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7141 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7201 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7261 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7321 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7381 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7441 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7501 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7561 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7621 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7801 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7861 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7921 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8341 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//