LOCUS       MR211721                8349 bp    DNA     circular  SYN 
DEFINITION  Cacna2d2 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 1
ACCESSION   NM_001174047
VERSION     NM_001174047.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4506..4597 
                     /label="Myc-DDK"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    4684..4703 
                     /label="Restriction Enzyme"  
     misc_feature    5488..6160 
                     /label="ColE1"  
     misc_feature    6590..7384 
                     /label="Kan/Neo"  
     misc_feature    7419..7721 
                     /label="SV40 ori"  
     misc_feature    7807..8284 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4488..4494 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4488 
                     /label="Cacna2d2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCTTC TTGGCCCGGC CCGGTGCGGA 
     1081 CCGCTCGCCC CTGGCCCGGT CGCGGCCCCC GGCCCTGCCC TGACCCCCGG GGCCCAGCGT 
     1141 CCGGGCCCGC ACGCCCGCTC TTGCTCTTGC TGCCGCCTCT GTTGCTTTTA CCGCTGCTCA 
     1201 CCGCCCCCGG CGCCTCTGCC TACAGCTTCC CCCAGCAGCA CACGATGCAG CACTGGGCCC 
     1261 GGCGCCTGGA GCAGGAGATT GACGGTGTGA TGCGGATTTT TGGAGGCGTG CAGCAGCTCC 
     1321 GAGAGATCTA CAAGGACAAT CGGAACCTGT TTGAAGTGCA GGAGAATGAA CCACAGAAAT 
     1381 TGGTGGAGAA AGTGGCAGGG GACATCGAGA GCCTGCTGGA CAGGAAGGTC CAGGCCCTGA 
     1441 AGAGACTGGC TGACGCTGCA GAGAATTTCC AGAAAGCCCA TCGCTGGCAG GACAACATCA 
     1501 AGGAGGAAGA CATCATGTAC TACGATGCCA AGGCTGACGC CGAGCTGGAT GACCCTGAGA 
     1561 GTGAAGATAT GGAGAGGGGA TCCAAGACCA GCGCCTTAAG GCTGGACTTC ATCGAGGACC 
     1621 CAAACTTCAA GAACAAAGTC AACTATTCAT ACACGGCTGT GCAGATCCCC ACAGACATCT 
     1681 ACAAAGGCTC TACCGTCATC CTCAATGAGC TTAACTGGAC AGAGGCCCTG GAGAACGTCT 
     1741 TCATTGAGAA CCGTAGGCAA GACCCTACAC TGTTGTGGCA AGTCTTTGGC AGTGCCACGG 
     1801 GAGTCACTCG CTATTACCCA GCCACACCAT GGCGAGCCCC CAAGAAGATT GACCTGTACG 
     1861 ATGTCAGAAG ACGACCCTGG TATATACAGG GGGCCTCATC ACCCAAGGAC ATGGTCATCA 
     1921 TTGTGGATGT GAGTGGCAGC GTGAGCGGCC TGACTCTGAA GCTGATGAAG ACGTCCGTCT 
     1981 GTGAGATGCT AGACACGCTC TCTGATGATG ACTATGTGAA CGTGGCCTCA TTCAACGAGA 
     2041 AGGCGCAGCC TGTGTCTTGC TTCACACACC TGGTGCAGGC CAATGTGCGT AACAAGAAGG 
     2101 TGTTCAAGGA AGCTGTGCAG GGCATGGTGG CCAAGGGCAC CACAGGCTAC AAGGCCGGCT 
     2161 TTGAGTATGC CTTTGACCAG CTACAGAATT CCAACATCAC GCGGGCTAAC TGCAATAAGA 
     2221 TGATCATGAT GTTCACGGAT GGGGGTGAGG ATCGCGTGCA GGATGTCTTC GAAAAGTACA 
     2281 ATTGGCCCAA TCGGACGGTA CGTGTGTTCA CGTTCTCCGT AGGACAGCAT AACTATGATG 
     2341 TCACACCCTT GCAGTGGATG GCCTGTACTA ACAAAGGTTA CTATTTTGAG ATCCCTTCCA 
     2401 TCGGAGCCAT CCGCATCAAC ACACAGGAAT ACCTGGATGT GCTGGGTAGG CCCATGGTAC 
     2461 TGGCAGGCAA GGACGCCAAG CAAGTGCAAT GGACAAACGT GTATGAAGAT GCACTGGGGC 
     2521 TGGGGTTGGT GGTAACAGGA ACTCTCCCTG TTTTCAACCT GACACAGGAT GGCCCTGGGG 
     2581 AAAAGAAGAA CCAGTTAATC CTGGGTGTCA TGGGCATCGA TGTGGCCTTG AATGACATCA 
     2641 AAAGGCTGAC TCCCAACTAC ACACTCGGCG CCAATGGCTA TGTGTTCGCC ATCGACCTGA 
     2701 ACGGCTACGT GTTGCTACAT CCCAATCTCA AGCCCCAGAC TACCAACTTC CGGGAGCCTG 
     2761 TGACCTTGGA CTTCCTGGAC GCAGAGCTGG AAGATGAGAA CAAGGAGGAG ATCCGTCGCA 
     2821 GCATGATTGA CGGCGACAAA GGCCACAAGC AGATCAGAAC CTTGGTCAAA TCCCTGGATG 
     2881 AGAGGTACAT AGACGAAGTG ATTCGGAACT ACACCTGGGT GCCTATAAGG AGTACCAACT 
     2941 ACAGCCTGGG GCTGGTGCTC CCACCCTACA GCACCTACTA CCTCCAAGCC AACCTCAGCG 
     3001 ACCAGATCCT GCAGGTCAAG TTGCCAATCA GCAAACTGAA GGATTTTGAG TTCCTGCTTC 
     3061 CCAGCAGCTT TGAGTCTGAA GGACACGTTT TCATTGCTCC GAGAGAGTAT TGCAAGGACT 
     3121 TGAACGCCTC GGACAACAAC ACCGAGTTCC TGAAGAACTT CATCGAGCTC ATGGAGAAAG 
     3181 TGACTCCGGA CTCCAAGCAG TGTAATAACT TCCTTCTTCA TAACTTGATT CTGGACACGG 
     3241 GCATTACACA GCAGTTAGTG GAACGTGTGT GGCGGGACCA AGATCTCAAC ACGTACAGCC 
     3301 TGCTAGCCGT ATTTGCTGCC ACTGATGGTG GCATCACACG TGTCTTCCCG AACAAGGCAG 
     3361 CCGAAGACTG GACAGAAAAC CCTGAACCCT TCAATGCCAG CTTCTACCGT CGCAGCCTGG 
     3421 ATAACCATGG TTATATCTTC AAGCCCCCAC ACCAGGACTC CCTGTTAAGA CCACTGGAGC 
     3481 TGGAGAATGA CACAGTAGGT GTCCTCGTCA GCACAGCTGT GGAGCTCAGT CTAGGTCGTC 
     3541 GCACACTGAG GCCAGCAGTG GTGGGTGTCA AACTGGACCT AGAGGCTTGG GCTGAAAAGT 
     3601 TCAAGGTGCT GGCCAGCAAC CGTACCCATC AGGACCAACC TCAGAAGTGC GGCCCCAGCA 
     3661 GCCACTGTGA GATGGACTGC GAGGTTAACA ACGAGGATCT ACTCTGTGTC CTCATTGATG 
     3721 ACGGAGGGTT CCTGGTGCTG TCAAACCAGA ACCATCAGTG GGACCAGGTT GGCAGATTCT 
     3781 TCAGTGAGGT GGATGCCAAC CTGATGCTGG CACTGTACAA TAACTCCTTC TACACCCGAA 
     3841 AGGAATCCTA TGACTATCAG GCAGCCTGTG CCCCTCAGCC TCCTGGGAAC CTGGGTGCTG 
     3901 CACCCCGGGG TGTCTTTGTG CCCACCATTG CAGATTTCCT TAACTTGGCC TGGTGGACCT 
     3961 CTGCTGCCGC CTGGTCCTTA TTCCAGCAGC TACTCTATGG TCTCATCTAT CACAGCTGGT 
     4021 TCCAGGCAGA CCCGGCAGAA GCTGAGGGCA GCCCCGAGAC GCGCGAGAGC AGCTGCGTCA 
     4081 TGAAACAGAC CCAGTACTAC TTCGGCTCGG TGAACGCATC CTATAATGCC ATCATTGACT 
     4141 GCGGAAACTG CAGCAGGCTG TTCCATGCGC AGAGACTGAC CAACACCAAT CTCCTGTTCG 
     4201 TGGTGGCCGA GAAGCCGCTG TGCAGCCAGT GCGAGGCGGG CCGGCTGCTG CAGAAGGAGA 
     4261 CACACTGCCC AGCGGACGGC CCGGAGCAGT GTGAGCTGGT GCAGAGACCG AGATACCGAA 
     4321 GAGGTCCGCA CATCTGTTTT GACTACAATG CGACGGAAGA TACCTCAGAC TGTGGCCGCG 
     4381 GAGCCTCCTT CCCTCCGTCG CTGGGCGTCT TGGTTTCCTT GCAGCTTTTG CTCCTCCTGG 
     4441 GCCTGCCACC TCGGCCGCAG CCTCAAGTCC ACTCCTTCGC TGCCTCTCGC CACCTCACGC 
     4501 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4561 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5281 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5341 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5461 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5521 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5581 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5641 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5761 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5821 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5881 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5941 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6061 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6121 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6181 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6241 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6301 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6361 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6421 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6481 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6541 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6601 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6661 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6721 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6781 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6841 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6901 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6961 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7021 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7081 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7141 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7201 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7261 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7321 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7381 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7441 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7501 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7561 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7921 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7981 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8041 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8101 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8161 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8221 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8281 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8341 GCGAATTTT
//