LOCUS       MR211710                8328 bp    DNA     circular  SYN 
DEFINITION  Lrrk1 (Myc-DDK-tagged) - Mouse leucine-rich repeat kinase 1 (cDNA clone MGC:99993 IMAGE:30543489)
ACCESSION   BC072664
VERSION     BC072664.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4485..4576 
                     /label="Myc-DDK"  
     misc_feature    4606..5191 
                     /label="PolyA signal"  
     misc_feature    4663..4682 
                     /label="Restriction Enzyme"  
     misc_feature    5467..6139 
                     /label="ColE1"  
     misc_feature    6569..7363 
                     /label="Kan/Neo"  
     misc_feature    7398..7700 
                     /label="SV40 ori"  
     misc_feature    7786..8263 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4467..4473 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4467 
                     /label="Lrrk1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCAAA CTGTCTTTAC CAGGTGGCAG CTCCACGGTG CAGTGCCCCA 
     1081 GCATGGAAGA AATCCACACT GCGTACAAGC AGAGGAACCT CTCCCGAGCC CGAGACCTAC 
     1141 TCAGAGGGGT TTGTGAAGAG AGCGAATCCT CACAGGAGAA GGGCCAGCTG CTGAGCATCG 
     1201 CGGCAGCCCA CGGGGATCTG GAGACTGTTC AGTTCCTGCT CACTGAGAAG CGCGTGGAGC 
     1261 TGCCGACGGA GCCCACCGAT GACAACCCAG CTGTGGTGGC AGCGCATTTT GGACACGCCG 
     1321 AAGTAGTACG TGAACTACTA GAGTCCTTGC CAGGTCCCTG TACCCCCCAG CGGCTACTGA 
     1381 ACTGGATGCT GGCCCTGGCT TGCCAGCGAG GGCACCTGGA GGTTGTGAAG CTGCTGGTCC 
     1441 TGACACATGG GGCTGACCCG GAGAACTATG CCGTCAGGAA GAATGAGTTC CCCGTCATTG 
     1501 TGCGGTTGCC CCTCTATGCG GCCATCAAGG CAGGGAATGA AGACATTGCC ATATTCCTGC 
     1561 TTCGGCACGG AGCCTATTTC TGCTCCTACA TCCTGCTGGA TAGTCCTGAG CCAAGCAAAC 
     1621 ATCTGCTCAG GAAGTATTTC ATCGAAGCCA GCGCCCTGCC CAGCAGCTAT CCCGGGAAAA 
     1681 TCGCACTTCG TGTGAAATGG TCCCATCTGA AGTTGCCCTG GGTGGACCTG GACTGGCTCC 
     1741 TAGACATCTC CTGCCAGATC ACAGAGCTTG ACCTGTCTGC CAACTGCCTG CCTTCGCTCC 
     1801 CTTCCATCAT CCCTTGGGGA CTCATCAATC TAAAGAAGCT GAATCTCTCA AACAACCAGC 
     1861 TGGGGGAGCT GCCCTGCGTG CAGTCCTCCG ACGAAATCAT CTGCTCCAGG TTACTTGAAA 
     1921 TTGACATCTC CAGCAACAAA CTGTCTCACC TCCCTCCTGG ATTCCTGCAC CTCTCAAAAC 
     1981 TTCAAAAGCT GACTGCTTCC AAAAACTACC TGGAGCGGCT GTTTGAAGAA GAAAACGCCA 
     2041 CTAACTGGAT CGGCCTGCGG AAGCTGCAAG AACTCGATCT AGCTGACAAC AGGCTGACAG 
     2101 AGCTCCCTGT CCAATTTATG CACTCCTTCA AGTCTCTCAC CTCTCTGAAT GTCTCCAGGA 
     2161 ACAACCTGAA GAGCTTCCCG GATCCCTGGT CCTGCCCTTT GAAATGTTGT AAAGCCTCCA 
     2221 AAAATGCCCT GGAATCGCTG CCAGACAAAA TGGCTGTCTT TTGGAAAAGC CACCTTCGGG 
     2281 ATGCAGATTT CTCTGAGAAC TCTCTGAAAG AGGTGCCCCT GGGATTGTTC CAGCTTGACG 
     2341 CCCTCATGTT CTTGAGGTTA CAGGGAAACC AGCTGTTGTC GCTGCCACCT CAAGAGAAGT 
     2401 GGACCTGCAC CCAACTCAAG ACCCTGGACC TCTCCAGAAA CCAGCTCGGC AAAAATGAAG 
     2461 ATGGGCTTAA AACAAAGAGG ATCTCCCTGT TCACCACCAG AGGGCGCCAG CGTTCTGGAA 
     2521 CTGAGACAGC CTCCATGTTG GAATTCCCAG CTTTCCTAAG TGAGTCTTTG GAGGTCCTTT 
     2581 GTCTGAATGA CAACCATCTT GATGCTGTTC CCCCATCGGT CTGCCTGCTG AAGAACCTCT 
     2641 CAGAGCTCTA CTTGGGCAAT AACCCTGGCC TCCGAGAGCT CCCTCCGGAG CTAGGCCAGC 
     2701 TGGGGAACCT CTGGCAACTG GACATTGAAG ATCTGAACAT TAGCAACGTG CCTGCGGAGG 
     2761 TCAGGAAAGA AGGCCCGAAA GCAACACTGT CTTTCCTGCG TGCTCAGCTG CGCAAAGCAG 
     2821 AGAAGTGTAA GCTGATGAAG ATGATCCTCG TGGGTCCCCC GCGCCAGGGC AAGTCCACAC 
     2881 TCCTGGAGAT CTTACAGACC GGGAAGGCCC CGCAGCTAGC ACACAGTGAA GCCACCATCA 
     2941 GGACCACCAA GTGGGAGCTC CAGAGACCAG CAGGCTCCAA AGCCAAGGTT GAGTCCGTAG 
     3001 AGTTCAACGT CTGGGACATC GGGGGTCCGG CCAGCATGGC CACGGTCAAT CAGTGCTTCT 
     3061 TCACAGACAA GGCGCTGTAT GTGGTGGTCT GGAACCTGGC ACTGGGAGAG GAAGCCGTGG 
     3121 CCAACCTCCA GTTCTGGCTG CTCAACATTG AGGCCAAGGC CCCAAATGCC GTTGTGCTGG 
     3181 TGGTCGGGAC ACACCTGGAC CTAATCGAAG CTAAGTTCCG AGTGGAGAGG ATAGCAACCC 
     3241 TGCGTGCCTA CGTGCTGGCC CTCTGCCGCT CGCCATCGGG CTCCAGGGCT ACGGGCTTCC 
     3301 CGGACATCAC TTTCAAACAC TTGCATGAGA TCTCCTGCAA GAATCTGGAA GGGCAGGAGG 
     3361 GGCTGCGGCA ACTGATCTTC CACGTCACGT GCAACATGAA GGATGTGGGT AGCACTATCG 
     3421 GCTGTCAAAA ACTCGCCGGC AGGCTGATCC CCAGGAGCTA CATAAGCCTA CAGGAGGCCG 
     3481 TGCTGGCTGA GCAACAGCGC CGCAGTCTGG GTGACCAAGT ACAGTATCTG ACGGACAGGC 
     3541 AGCTGGACCA GCTGGTAGAG CAGACGCCTG GCAATGATAT CAAAGACTAT GAAGACCTGC 
     3601 AGTCTGCCAT CAGCTTCCTC ATAGAAACTG GGACCCTGCT GCACTTTCCA GACACGAGCC 
     3661 ATGGCCTAAG GAACCTCTAC TTCCTGGATC CCATCTGGCT CTCTGAATGC CTGCAGAGGA 
     3721 TCTTTAACAT CAAGGGCTCG CGGTCAGTGG CAAAGAATGG GGTGATCCAA GCGGAAGACC 
     3781 TCAGGATGCT GCTGGTGGGG ACAGGCTTCA CACAGCAGAC TGAGGAGCAA TACTTCCAGT 
     3841 TCCTTGCCAA GTTCGAGATT GCCCTCCCTG TTGCCAATGA CAGCTACCTC CTACCACACC 
     3901 TCCTTCCATC CAAGCCTGGG CTGGACACCC ACAGCATGCG GCACCCAATG GCTAACACCA 
     3961 TCCAGCGGGT GTTTAAGATG AGCTTCGTGC CTGTTGGCTT CTGGCAACGG TTCATAGCAC 
     4021 GGATGCTAAT CAGCTTGGCT GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACGA 
     4081 AAAGCAGGAA CCGGAAAGTC ACTATTTACA GTTTTACAGG GAGCCAGAGA AACCGCTGCA 
     4141 GTACATTCCG AGTCCGAAGA AATCAGACCA TCTACTGGCA GGAAGGGCTG CTGGTCACTT 
     4201 TCGATGGGGG CTACCTCAGT GTGGAATCCT CAGATGTGAA CTGGAAAAAG AAAAAAAGCG 
     4261 GAGGAATTAA AATCATCTGC CAGTCAGAGA TGAGGGACTT CTCGGCAATG GCTTTTATCA 
     4321 CAGACCATGT CAACTCCCTG ATTGACCAGT GGTTCCCTGC CCTGACGGCC ACAGAGTGGT 
     4381 GCCTGGCTGT CTGGAGGGGC TGGGGCGCCA GGGAGTTTGA CATCTTCTAC CAGCTCCCTC 
     4441 ACCAGCTATT CCAGTGTGCC TTTCTCTGCC AACTAACGCG TACGCGGCCG CTCGAGCAGA 
     4501 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4561 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4621 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4681 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4741 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4801 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4861 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4921 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4981 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5041 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5101 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5161 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5221 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5281 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5341 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5401 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5461 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5521 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5581 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5641 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5701 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5761 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5821 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5881 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5941 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6001 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6061 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6121 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6181 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6241 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6301 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6361 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6421 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6481 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6541 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6601 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6661 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6721 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6781 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6841 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6901 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6961 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7021 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7081 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7141 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7201 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7261 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7321 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7381 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7441 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7501 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7561 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7741 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7801 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7861 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7921 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7981 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8041 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8101 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8161 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8221 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8281 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//